Standard name
Description Protein that interacts with Tap42p, which regulates PP2A; component of the TOR (target of rapamycin) signaling pathway; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 0.96 0.9 0.86 0.74 0.69 0.63 0.6 0.62 0.54 0.99 1.0 0.99 0.92 0.93 0.97 0.94 0.96 0.94 0.92 0.94 0.91
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.15 0.14 0.23 0.15 0.34 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0.09 0.12 0.18 0.18 0.18 0.28 0.12 0.32 0 0 0 0.1 0.12 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud 2 1 0 0 1 1 3 5 2 6 5 3 0 0 0 0 0 0 1 0 0 1 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 3 4 4 5 1 5 3 9 5 7 6 8 0 1 3 23 5 8 0 0 0 0 0 0
Cytoplasm 282 326 299 290 346 275 306 307 183 248 180 173 187 286 282 481 214 386 270 326 287 201 195 191
Endoplasmic Reticulum 1 0 3 0 3 1 1 3 2 1 3 2 2 0 2 9 3 13 0 0 3 4 1 4
Endosome 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 1 2 0 5 1 3 1 1 1
Golgi 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 0 4 0 2 0 0 0
Mitochondria 6 1 0 2 9 17 64 60 67 63 98 62 0 0 0 1 0 0 2 2 1 0 1 0
Nucleus 2 2 0 2 2 6 11 21 13 16 7 10 1 0 0 2 2 6 0 1 0 1 0 0
Nuclear Periphery 2 0 0 0 0 0 1 0 1 0 0 1 0 0 0 0 0 0 0 0 0 0 0 2
Nucleolus 0 0 0 0 0 1 1 1 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1
Vac/Vac Membrane 0 3 2 15 35 37 74 79 51 115 36 102 0 0 0 51 27 13 0 3 1 1 1 1
Unique Cell Count 290 331 304 303 384 318 414 442 290 414 289 320 189 286 284 524 230 400 288 339 306 218 208 211
Labelled Cell Count 299 339 308 314 397 343 464 487 326 457 335 362 190 287 287 569 254 426 288 339 306 218 208 211


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.1 9.1 8.4 7.6 7.7 7.0 6.9 7.3 6.7 7.2 6.5 7.2 9.1 9.5 10.0 13.1 13.6 12.6 9.0 8.6 9.4
Std Deviation (1e-4) 0.8 1.3 1.2 1.2 1.3 1.4 1.4 1.3 1.3 1.3 1.5 1.5 1.3 1.3 1.2 2.6 3.3 2.8 1.8 1.3 1.6
Intensity Change (Log2) – – – -0.14 -0.13 -0.27 -0.28 -0.2 -0.32 -0.22 -0.36 -0.22 0.12 0.18 0.25 0.65 0.7 0.59 0.1 0.03 0.16


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0.7 0 0 0 1.1 0 0 0 2.4 0 0.7
Cytoplasm -1.9 -4.4 -5.5 -8.9 -9.9 -11.0 -12.0 -11.1 -12.9 0.5 2.2 1.1 -3.9 -3.1 -1.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.1 7.2 6.7 8.9 7.1 11.1 8.1 0 0 0 0 0 0
Nucleus 0 0 0 0 3.9 3.7 3.5 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 3.2 4.9 5.6 7.4 7.4 7.2 9.7 5.9 10.5 0 0 0 5.1 5.6 2.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.004 4.8938 4.397 4.1318 3.6556 4.5909 3.3074 4.0415 4.0816 3.5924 3.5724 3.9172 3.3885 4.2246 3.8301 4.1436 3.9528 3.6634
Actin 0.0115 0.0062 0.0042 0.001 0.0182 0.0025 0.001 0.0006 0.0006 0.0003 0.0011 0.0016 0.0226 0.0042 0.0042 0.0007 0.0268 0.0021
Bud 0.0013 0.0008 0.001 0.0003 0.0064 0.0005 0.0013 0.0024 0.0009 0.0001 0.0052 0.0043 0.0006 0.0012 0.0011 0.0003 0.0021 0.0014
Bud Neck 0.0153 0.0005 0.0008 0.0005 0.0007 0.001 0.0004 0.0004 0.0005 0.0004 0.0005 0.0016 0.0097 0.0004 0.0006 0.0004 0.0012 0.0008
Bud Periphery 0.0021 0.0005 0.001 0.0001 0.0113 0.0005 0.0005 0.0007 0.0004 0 0.0038 0.0022 0.0009 0.0004 0.002 0.0001 0.0014 0.001
Bud Site 0.0093 0.0048 0.0069 0.0002 0.0027 0.0004 0.0049 0.0104 0.002 0.0001 0.0002 0.0008 0.0063 0.0036 0.0057 0.0003 0.0009 0.0001
Cell Periphery 0.0006 0.0002 0.0001 0 0.0005 0 0.0001 0.0001 0.0001 0 0 0.0001 0.0004 0.0001 0.0009 0 0.0001 0
Cytoplasm 0.7132 0.9127 0.8224 0.827 0.6941 0.8391 0.8865 0.9183 0.9204 0.9098 0.8433 0.9147 0.8249 0.9383 0.8984 0.9438 0.8216 0.9366
Cytoplasmic Foci 0.0364 0.014 0.0232 0.0247 0.0287 0.0186 0.0068 0.0043 0.0058 0.0067 0.0089 0.0107 0.0088 0.0065 0.0105 0.0047 0.0124 0.0105
Eisosomes 0.0002 0 0.0001 0 0.0006 0 0 0 0 0 0 0 0.0002 0.0001 0.0001 0 0.0002 0
Endoplasmic Reticulum 0.0067 0.0064 0.0026 0.0011 0.0035 0.0023 0.0041 0.0013 0.0025 0.0031 0.0014 0.0026 0.0072 0.003 0.0032 0.0016 0.0093 0.0018
Endosome 0.028 0.0061 0.0142 0.0062 0.0507 0.0148 0.0034 0.0017 0.0045 0.0083 0.0031 0.004 0.0126 0.0028 0.004 0.0047 0.0267 0.0047
Golgi 0.0062 0.0012 0.007 0.0037 0.0156 0.0041 0.0002 0.0001 0.0002 0.0002 0.0001 0.0002 0.0028 0.0007 0.0007 0.0002 0.0055 0.0008
Lipid Particles 0.0046 0.0002 0.0023 0.0054 0.0041 0.0017 0.0002 0 0 0 0 0.0001 0.0035 0.0004 0.0016 0.0001 0.0096 0.0004
Mitochondria 0.0091 0.0008 0.0083 0.0012 0.0393 0.008 0.0004 0.0002 0.0006 0.0003 0.0003 0.0003 0.0031 0.0013 0.0041 0.0004 0.0108 0.0051
None 0.1178 0.0321 0.0842 0.0596 0.1019 0.0874 0.0772 0.0482 0.0509 0.06 0.1125 0.0421 0.0532 0.0243 0.0488 0.0133 0.019 0.0224
Nuclear Periphery 0.0043 0.0014 0.0018 0.0011 0.0025 0.0044 0.0019 0.0009 0.0012 0.0016 0.0018 0.0017 0.0154 0.0033 0.0025 0.0051 0.0189 0.0012
Nucleolus 0.0016 0.0013 0.0008 0.0001 0.0002 0.0001 0.0002 0.0001 0.0001 0 0.0002 0.0001 0.002 0.0003 0.0004 0.0002 0.0011 0
Nucleus 0.0114 0.0065 0.0063 0.009 0.005 0.0068 0.007 0.0077 0.0061 0.0059 0.0101 0.0091 0.0156 0.0055 0.0053 0.0119 0.0163 0.0076
Peroxisomes 0.0097 0.0012 0.0068 0.055 0.0029 0.0035 0.0002 0.0001 0.0001 0.0001 0.0002 0.0001 0.002 0.0005 0.0021 0.0002 0.001 0.0005
Punctate Nuclear 0.0058 0.0018 0.004 0.0016 0.0057 0.0027 0.0022 0.0016 0.0017 0.0008 0.006 0.0023 0.0044 0.0024 0.0027 0.0109 0.0104 0.0011
Vacuole 0.0039 0.0012 0.0015 0.0019 0.0026 0.0011 0.0011 0.0008 0.0012 0.0015 0.0008 0.0012 0.0026 0.0007 0.001 0.0007 0.0022 0.0013
Vacuole Periphery 0.001 0.0002 0.0005 0.0003 0.0028 0.0005 0.0002 0.0001 0.0002 0.0007 0.0002 0.0002 0.0014 0.0002 0.0002 0.0002 0.0024 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.09 11.0518 21.1858 18.1879 23.376 21.6234 19.3226 22.6261 17.0598 21.526
Translational Efficiency 1.3214 2.3584 0.9662 1.2973 1.0015 1.3956 1.5615 1.597 1.9058 1.2631

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘