Standard name
Human Ortholog
Description Component of the Ctf19 complex and the COMA subcomplex; involved in kinetochore-microtubule mediated chromosome segregation; binds to centromere DNA; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-H and fission yeast fta3
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – 0 0 0 0.05 0 0.1 0.07 0.1 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – 0 0.11 0.07 0 0.06 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – 0 0 0.06 0 0 0.08 0.08 0.05 0.07 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.11 0.1 – 0.25 0.16 0.21 0.22 0.17 0.17 0.13 0.18 0.12 0.18 0.21 0.29 0.2 0.28 0.3 0.08 0.07 0 0.11 0.12 0.17
Endoplasmic Reticulum 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.36 0.49 – 0.25 0.15 0.05 0.16 0.17 0.1 0.06 0.06 0.07 0.35 0.44 0.4 0.51 0.28 0.33 0.08 0.12 0.16 0.12 0.13 0.11
Golgi 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 – 0.06 0 0.2 0 0.11 0.33 0.26 0.39 0.37 0 0 0 0 0.05 0.05 0 0 0 0 0 0
Nucleus 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.09 0 – 0 0 0.12 0 0 0 0 0.11 0.12 0 0.05 0 0.07 0.05 0.09 0 0 0 0 0 0
Peroxisomes 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0.06 0 0 0 0 0 0 0 0
SpindlePole 0.61 0.48 – 0.56 0.75 0.54 0.68 0.64 0.53 0.6 0.44 0.51 0.63 0.51 0.47 0.48 0.46 0.39 0.73 0.68 0.75 0.65 0.62 0.56
Vac/Vac Membrane 0.08 0.1 – 0.06 0 0 0 0 0 0 0 0 0 0 0 0.11 0.1 0.06 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – 0 3 2 0 2 0 4 0 2 2 1 1 1 1 1 2 1 0 5 7 13
Bud 4 4 – 1 3 2 9 9 13 10 6 4 3 4 4 5 3 2 4 5 1 11 17 19
Bud Neck 0 1 – 1 17 7 7 14 5 9 3 2 18 25 8 1 3 1 1 6 0 5 8 15
Bud Site 2 1 – 0 4 6 3 9 10 12 3 6 3 3 2 2 0 4 – – – – – –
Cell Periphery 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Cytoplasm 33 24 – 8 26 21 38 37 23 19 11 10 122 182 263 39 43 43 27 19 0 63 101 129
Endoplasmic Reticulum 3 0 – 0 1 1 0 0 0 0 0 0 2 0 0 9 5 5 2 0 0 1 2 3
Endosome 110 119 – 8 23 5 27 37 13 9 4 6 240 375 373 101 42 47 28 34 8 72 109 83
Golgi 0 1 – 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 2 0 0 2 3
Mitochondria 22 5 – 2 1 20 5 24 44 37 24 31 6 5 4 6 7 7 9 5 0 19 27 24
Nucleus 1 2 – 0 1 4 2 3 4 6 3 0 8 4 7 2 3 0 0 1 0 0 2 2
Nuclear Periphery 1 0 – 0 0 1 1 0 0 0 1 0 1 0 0 0 1 0 1 0 0 1 2 1
Nucleolus 26 9 – 1 5 12 3 7 5 6 7 10 26 40 37 13 7 13 3 7 0 1 5 2
Peroxisomes 1 2 – 0 2 1 7 4 2 4 3 3 6 37 31 11 1 5 0 0 0 5 1 1
SpindlePole 185 117 – 18 118 55 119 143 70 87 27 43 434 440 432 97 70 56 261 198 40 382 526 432
Vac/Vac Membrane 23 24 – 2 3 1 1 2 4 1 0 1 25 37 40 21 15 9 6 3 0 12 14 14
Unique Cell Count 304 243 32 158 101 174 223 133 145 62 84 691 862 922 200 152 142 357 290 54 592 851 770
Labelled Cell Count 411 309 41 207 138 222 291 193 204 92 118 897 1153 1202 308 201 193 357 290 54 592 851 770


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.6 5.1 – 5.2 6.3 4.1 5.2 5.0 4.0 4.3 4.0 3.9 5.3 5.2 5.3 6.3 6.0 6.2 4.7 4.8 4.8
Std Deviation (1e-4) 0.9 0.8 – 0.5 1.5 1.5 2.0 2.1 0.7 1.2 1.4 0.8 1.0 1.0 1.1 1.5 1.0 1.6 1.1 1.3 1.5
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration – – – – – – – – – – – – – – – – – –
Actin 0.0181 0.0007 0.0227 0.0099 0.0346 0.0082 0.0485 0.0007 0.0077 0.0064 0.0036 0.0121 0.1241 0.0027 0.0334 0.0031 0.0001 0.0061
Bud 0.0003 0.0007 0.0003 0.0002 0.0005 0.0003 0.0013 0.0004 0.0002 0.0001 0.0002 0.0002 0.0053 0.0051 0.0002 0.002 0 0.0002
Bud Neck 0.0059 0.0013 0.0037 0.0024 0.0044 0.0035 0.0086 0.0011 0.0079 0.0034 0.0011 0.0022 0.0027 0.0016 0.0014 0.0025 0 0.0018
Bud Periphery 0.0005 0.0004 0.0004 0.0002 0.0014 0.001 0.0019 0.0003 0.0001 0.0001 0.0004 0.0002 0.0038 0.0037 0.0002 0.0031 0 0.0002
Bud Site 0.0045 0.0057 0.0045 0.001 0.0013 0.0005 0.0042 0.0054 0.0019 0.0005 0.0014 0.0004 0.0167 0.0129 0.0015 0.0016 0 0.0002
Cell Periphery 0.0002 0.0001 0.0002 0 0.0001 0 0.0004 0.0001 0.0001 0 0.0001 0 0.001 0.0001 0 0.0001 0 0
Cytoplasm 0.0369 0.1182 0.0495 0.0395 0.0361 0.0394 0.0692 0.2125 0.1061 0.1074 0.0109 0.0567 0.029 0.1451 0.0727 0.0524 0.0013 0.0895
Cytoplasmic Foci 0.1286 0.1338 0.094 0.2187 0.3018 0.1027 0.1354 0.1197 0.1055 0.1312 0.3324 0.0832 0.2028 0.1757 0.1485 0.2121 0.6086 0.1204
Eisosomes 0.0007 0.0001 0.0002 0.0002 0.0002 0.0001 0.0006 0.0001 0.0001 0.0001 0.0004 0.0001 0.001 0 0.0001 0 0 0.0001
Endoplasmic Reticulum 0.0039 0.0024 0.0025 0.0017 0.0089 0.001 0.0039 0.0039 0.0042 0.0037 0.0006 0.0014 0.0035 0.0031 0.0011 0.0018 0 0.0034
Endosome 0.0346 0.0279 0.0239 0.0314 0.0503 0.0152 0.04 0.0291 0.02 0.0117 0.0602 0.0183 0.0269 0.0311 0.0079 0.0195 0.0069 0.0131
Golgi 0.0059 0.0017 0.004 0.0047 0.0114 0.0026 0.0107 0.0025 0.0032 0.0019 0.0067 0.004 0.0106 0.0018 0.003 0.0019 0.0012 0.0021
Lipid Particles 0.0216 0.0225 0.0173 0.03 0.0684 0.0184 0.0247 0.0087 0.0065 0.0056 0.0407 0.0113 0.05 0.0115 0.0073 0.004 0.0482 0.006
Mitochondria 0.0057 0.0005 0.0101 0.0008 0.002 0.0013 0.0079 0.0008 0.0011 0.0004 0.0152 0.0015 0.0053 0.0004 0.0008 0.0008 0 0.0007
None 0.1171 0.1738 0.1158 0.1294 0.0052 0.0867 0.0691 0.162 0.0799 0.0436 0.0516 0.0521 0.1056 0.1959 0.1435 0.0999 0.0014 0.0939
Nuclear Periphery 0.0232 0.0085 0.0137 0.0119 0.0348 0.01 0.0178 0.0087 0.0096 0.0103 0.0014 0.0153 0.0189 0.0055 0.0031 0.0068 0.0006 0.0114
Nucleolus 0.0062 0.006 0.0066 0.0024 0.0036 0.0042 0.0071 0.0091 0.0051 0.0079 0.002 0.0048 0.0036 0.0029 0.0032 0.0026 0.0001 0.0024
Nucleus 0.035 0.0586 0.0457 0.0081 0.0053 0.0268 0.0553 0.0665 0.0717 0.0736 0.0028 0.0492 0.0266 0.0245 0.0529 0.0187 0.0001 0.0291
Peroxisomes 0.0103 0.0087 0.0115 0.0194 0.034 0.0091 0.0183 0.007 0.0072 0.0068 0.0571 0.0073 0.0219 0.0047 0.0094 0.0072 0.0119 0.0089
Punctate Nuclear 0.5377 0.4256 0.5716 0.4866 0.3932 0.6678 0.468 0.3575 0.5601 0.584 0.4006 0.6779 0.3367 0.3686 0.5087 0.5585 0.3196 0.6094
Vacuole 0.002 0.0025 0.0012 0.0009 0.0014 0.0008 0.0051 0.0034 0.0016 0.0009 0.0101 0.0011 0.0025 0.0028 0.0009 0.0011 0 0.0008
Vacuole Periphery 0.0013 0.0004 0.0005 0.0005 0.0012 0.0003 0.0018 0.0006 0.0004 0.0003 0.0007 0.0006 0.0014 0.0002 0.0001 0.0003 0 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.1149 10.8393 9.3838 10.6212 9.1706 6.0593 14.1562 22.8074 8.3659 12.1408
Translational Efficiency 1.2144 1.0472 0.8951 0.7211 0.9446 1.9854 1.0904 0.5421 1.4603 1.2673

Hit Data

Dataset Hit
Protein Concentration –
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1296 1060 440 295 2012 2288 450 1546 3308 3348 890 1841

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 677.23 732.77 883.13 889.78 664.18 778.92 882.02 836.09 669.29 764.31 882.57 844.69
Standard Deviation 73.10 107.31 109.43 118.74 83.01 100.11 96.76 109.04 79.53 104.67 103.22 112.39
Intensity Change Log 2 — 0.113715 0.382980 0.393803 — 0.229901 0.409237 0.332084 — 0.172412 0.396041 0.363574

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000173 0.000522 0.000976 0.000992 0.000117 0.000324 0.001042 0.000735 0.000139 0.000387 0.001010 0.000776
Bud Neck 0.009196 0.011991 0.014871 0.004649 0.012157 0.026537 0.004160 0.007582 0.010997 0.021931 0.009455 0.007112
Bud Site 0.004884 0.011954 0.010893 0.039004 0.002978 0.015908 0.012180 0.042366 0.003724 0.014657 0.011544 0.041827
Cell Periphery 0.000152 0.000111 0.000142 0.000105 0.000130 0.000186 0.000402 0.000110 0.000138 0.000163 0.000274 0.000109
Cytoplasm 0.198661* 0.208214* 0.047283 0.167897* 0.240422* 0.296524* 0.072648 0.234259* 0.224061* 0.268564* 0.060108 0.223625*
Cytoplasmic Foci 0.405543* 0.223131* 0.005546 0.025669 0.356847* 0.206293* 0.019165 0.036385 0.375925* 0.211624* 0.012432 0.034668
Eisosomes 0.000097 0.000068 0.000084 0.000050 0.000086 0.000072 0.000070 0.000029 0.000090 0.000071 0.000077 0.000032
Endoplasmic Reticulum 0.000592 0.000823 0.005198 0.002666 0.000345 0.000635 0.003477 0.000662 0.000442 0.000695 0.004328 0.000983
Endosome 0.015599 0.030739 0.007640 0.007911 0.013622 0.029648 0.010632 0.015788 0.014397 0.029993 0.009153 0.014525
Golgi 0.006989 0.009742 0.000575 0.000846 0.009018 0.007010 0.003813 0.003601 0.008223 0.007875 0.002212 0.003160
Lipid Particles 0.068323 0.013837 0.003234 0.003033 0.032241 0.017349 0.003275 0.001717 0.046377 0.016237 0.003254 0.001928
Mitochondria 0.008093 0.008250 0.001798 0.000283 0.011844 0.011557 0.001441 0.005928 0.010375 0.010510 0.001617 0.005023
Mitotic Spindle 0.094310 0.317677* 0.617358* 0.558866* 0.111890* 0.144218* 0.643632* 0.513434* 0.105003* 0.199136* 0.630643* 0.520714*
None 0.020663 0.015387 0.005538 0.008653 0.031331 0.023398 0.005223 0.005661 0.027151 0.020862 0.005379 0.006141
Nuclear Periphery 0.000915 0.000747 0.001519 0.001120 0.000901 0.001141 0.000988 0.001254 0.000906 0.001016 0.001251 0.001232
Nuclear Periphery Foci 0.015282 0.015544 0.032844 0.033405 0.008590 0.006192 0.035246 0.003812 0.011212 0.009153 0.034059 0.008554
Nucleolus 0.042760 0.025880 0.030872 0.003530 0.054733 0.043061 0.008121 0.002656 0.050042 0.037622 0.019369 0.002796
Nucleus 0.074257 0.056011 0.184356* 0.083957 0.083384 0.130918* 0.148021* 0.060022 0.079808 0.107202 0.165984* 0.063857
Peroxisomes 0.027313 0.029144 0.002710 0.003564 0.025593 0.021529 0.002958 0.005918 0.026267 0.023940 0.002835 0.005540
Vacuole 0.005478 0.018387 0.025714 0.053083 0.003321 0.016198 0.022458 0.044075 0.004166 0.016891 0.024068 0.045519
Vacuole Periphery 0.000718 0.001840 0.000849 0.000716 0.000450 0.001302 0.001048 0.014007 0.000555 0.001472 0.000950 0.011877

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.78 -10.27 -8.17 -5.74 1.72 -4.51 -8.57 -11.01 -7.07 4.50 -7.87 -13.25 -13.40 -8.03 7.03
Bud Neck -4.59 -6.79 -4.54 -0.21 3.67 -9.87 -1.12 -4.69 6.91 -2.56 -11.09 -6.19 -6.94 5.97 2.03
Bud Site -8.09 -6.88 -6.16 -4.14 -2.04 -8.87 -7.90 -10.60 -6.29 -1.99 -11.45 -10.44 -12.04 -7.38 -2.48
Cell Periphery -1.18 -5.71 -5.39 -4.78 -0.05 -5.03 -2.41 -0.95 4.19 2.20 -4.94 -4.79 -2.51 2.52 3.82
Cytoplasm -6.03 9.28 -0.82 3.46 -5.97 -11.53 10.91 -6.86 3.27 -15.69 -13.42 14.79 -7.91 3.04 -18.38
Cytoplasmic Foci 17.13 46.53 42.48 24.09 -3.46 10.14 46.05 43.60 35.32 -5.47 18.00 65.73 58.30 40.89 -8.32
Eisosomes 1.79 -6.42 -4.72 -5.25 1.15 0.58 -3.59 1.85 1.47 4.26 1.90 -7.40 -0.15 -1.39 6.88
Endoplasmic Reticulum -7.50 -12.55 -9.14 -8.28 0.72 -5.37 -9.57 -15.70 -12.59 6.59 -8.78 -15.67 -14.95 -12.51 9.24
Endosome -7.61 1.97 0.22 6.47 -1.25 -7.70 2.16 1.19 7.71 -1.03 -10.61 2.96 1.15 9.86 -1.47
Golgi -0.77 14.90 17.15 10.22 4.63 2.59 9.14 11.45 9.55 1.34 2.00 14.67 17.07 13.38 2.46
Lipid Particles 10.50 14.50 13.95 6.86 -2.08 4.11 14.41 15.00 12.27 1.03 10.25 20.09 20.40 14.48 0.76
Mitochondria -0.73 5.44 7.34 4.29 4.89 -1.88 6.92 4.77 7.50 -3.86 -2.23 8.41 6.14 7.99 -2.63
Mitotic Spindle -14.45* -14.85 -11.37 -3.14 0.83 1.06 -22.23* -28.01* -29.17* 4.12 -7.73 -25.50 -31.67 -25.25 1.56
None 1.78 7.17 3.48 1.79 -1.07 1.11 11.60 11.93 11.47 -0.73 1.65 13.41 13.22 10.97 -0.29
Nuclear Periphery 1.42 -10.06 -7.83 -8.13 -1.35 -2.42 -6.11 -12.17 -10.63 -2.66 -1.68 -11.28 -14.47 -13.43 -2.05
Nuclear Periphery Foci 2.66 -3.87 -4.10 -5.25 -1.02 1.43 -7.19 2.95 1.51 8.14 3.40 -8.34 1.58 -1.19 8.80
Nucleolus 9.20 -0.31 15.37 7.27 10.21 6.52 14.97 23.95* 18.97 4.46 9.67 7.91 28.79* 20.01 10.91
Nucleus -2.58 -11.21 -6.54 -5.48 4.22 -10.17 -5.56 -7.83 1.22 2.04 -10.52 -11.97 -10.65 -2.48 6.32
Peroxisomes -1.86 16.16 12.28 11.69 0.25 1.82 16.97 14.88 13.54 -2.18 0.53 23.24 19.50 17.74 -2.04
Vacuole -6.40 -7.17 -9.95 -9.14 -7.65 -8.84 -6.53 -16.58 -14.31 -6.68 -10.75 -9.70 -18.99 -16.58 -9.89
Vacuole Periphery -3.72 -1.87 -0.44 3.50 1.41 -6.08 -0.90 -2.76 -2.13 -2.65 -6.74 -2.14 -2.75 -1.92 -2.56