Standard name
Description snRNP protein component of spliceosomal snRNPs; required for pre-mRNA splicing and snRNP biogenesis; in null mutant newly-synthesized snRNAs are destabilized and 3'-end processing is slowed

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0.05 0.06 0.08 0.07 0.07 0.11 0.07 0.07 0.1 0.11 0.13 0.23 0.2 0.1 0.16 0.09 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.08 0.05 0.13 0.61 0.56 0.52 0.57 0.63 0.72 0.57 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.95 0.93 0.9 0.89 0.81 0.67 0.66 0.59 0.53 0.53 0.49 0.43 0.85 0.73 0.74 0.8 0.74 0.8 0.92 0.88 0.85 0.66 0.53 0.48
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.07 0.09 0.07 0.16 0.12 0.1 0.17 0.06 0.07 0 0.12 0.15 0.12 0 0.07 0.09 0.24 0.29 0.36
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 2 1 10 5 6 9 4 2 0 0 0 0 0 0 2 7 2 0 0 2
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 2 0
Bud Site 0 0 0 2 1 5 13 9 6 8 11 4 0 0 0 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 10 23 20 19 27 39 42 68 37 40 48 54 16 26 28 12 19 9 1 7 3 1 2 2
Endoplasmic Reticulum 0 2 3 0 0 0 0 0 0 0 0 0 1 0 0 5 1 4 0 1 0 0 1 0
Endosome 0 1 4 2 5 0 1 5 1 2 1 7 0 1 4 0 1 0 0 2 2 0 3 2
Golgi 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 1 0
Mitochondria 7 25 35 16 46 339 358 327 287 368 357 274 3 0 0 4 3 1 2 4 4 4 5 5
Nucleus 210 567 388 271 280 369 424 368 266 312 242 208 106 83 102 100 87 78 200 552 368 91 100 98
Nuclear Periphery 0 0 4 4 2 1 3 6 0 3 2 2 0 0 1 0 0 0 0 0 1 0 2 3
Nucleolus 0 0 0 1 1 4 5 5 3 1 5 1 0 1 0 0 0 0 0 0 2 2 5 6
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 2 0 0 2 0 5 0 2 0 1 1 0 0 1 1 5 0 0 1 5
Vac/Vac Membrane 1 27 17 11 18 38 57 44 81 73 50 81 7 8 6 15 17 12 8 41 38 33 55 72
Unique Cell Count 220 610 429 305 345 552 641 625 501 585 493 481 124 114 137 125 117 97 218 630 433 139 191 204
Labelled Cell Count 228 647 471 326 384 796 913 839 687 822 720 635 133 120 143 136 129 105 218 630 433 139 191 204


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.4 5.1 5.1 4.7 3.8 3.8 4.3 3.9 3.6 3.5 3.7 5.7 5.9 6.0 7.1 7.0 7.1 5.2 5.9 5.7
Std Deviation (1e-4) 0.7 0.9 1.4 1.7 1.5 1.8 1.3 1.5 1.3 1.0 1.1 0.8 1.0 1.0 1.0 1.3 1.5 1.2 0.8 1.2 1.0
Intensity Change (Log2) 0.02 -0.11 -0.41 -0.41 -0.25 -0.37 -0.47 -0.53 -0.47 0.17 0.22 0.24 0.48 0.46 0.48 0.03 0.21 0.18


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.9 1.8 1.6 1.3 3.6 1.7 1.5 2.9 3.6 3.3 6.2 5.8 2.1 4.3 1.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria -1.5 0 0 0 0 0 0 0 0 -2.2 -3.2 -3.5 -1.9 -2.1 -2.5
Nucleus -0.7 -3.7 -8.7 -9.1 -11.2 -12.4 -12.6 -13.5 -15.0 -1.6 -4.9 -4.8 -3.2 -4.6 -2.8
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -0.2 0.8 2.0 3.1 2.1 6.0 4.7 3.6 6.3 0.8 1.4 0.2 3.4 4.2 3.3