Standard name
Human Ortholog
Description Spermidine synthase; involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm – 0.98 0.98 0.96 0.97 0.89 0.81 0.81 0.86 0.78 0.82 0.72 1.0 1.0 1.0 – – – 0.93 0.95 0.95 0.76 0.86 0.87
Endoplasmic Reticulum – 0 0 0 0 0 0 0.06 0 0 0.06 0.05 0 0 0 – – – 0 0 0 0 0 0
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Golgi – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Mitochondria – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0.05 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0.05 0.15 0.11 0.06 0.14 0.08 0.17 0 0 0 – – – 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0 0 0 2 0 0 2 1 2 5 0 0 0 – – – 0 0 0 0 0 0
Bud – 0 0 0 0 0 2 1 1 1 0 1 0 0 0 – – – 0 0 0 1 1 3
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery – 0 6 1 3 9 7 9 4 11 8 14 0 0 1 – – – 0 0 0 0 1 1
Cytoplasm – 156 170 115 250 221 211 248 205 207 186 225 96 125 156 – – – 48 150 152 51 178 200
Endoplasmic Reticulum – 0 2 4 2 7 8 18 10 7 14 17 0 0 0 – – – 0 0 0 2 0 2
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 2 1
Golgi – 0 0 0 0 0 2 5 0 4 1 8 0 0 0 – – – 0 0 0 0 0 1
Mitochondria – 1 0 0 0 0 0 1 4 2 3 0 0 0 0 – – – 0 0 1 0 1 1
Nucleus – 1 1 1 0 4 1 10 4 5 2 10 0 0 0 – – – 0 3 2 3 8 4
Nuclear Periphery – 0 0 0 0 0 0 0 1 0 0 0 0 0 0 – – – 0 0 0 1 1 1
Nucleolus – 0 0 0 0 0 0 0 0 0 0 1 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane – 2 3 2 7 13 40 33 14 37 19 52 0 0 0 – – – 1 1 0 2 5 2
Unique Cell Count 159 173 120 258 247 259 306 237 266 228 314 96 125 156 52 158 161 67 208 229
Labelled Cell Count 160 182 123 262 256 271 325 245 275 235 333 96 125 157 52 158 161 67 208 229


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 28.4 28.6 29.6 29.4 23.9 22.9 21.4 19.3 20.1 19.4 20.9 31.7 29.9 29.6 – – – 17.3 23.9 24.7
Std Deviation (1e-4) – 6.0 6.5 6.4 5.4 5.1 5.0 4.9 4.5 4.3 4.8 5.4 6.9 6.2 6.7 – – – 7.8 9.2 9.3
Intensity Change (Log2) – – – 0.05 0.04 -0.26 -0.32 -0.42 -0.57 -0.51 -0.56 -0.46 0.15 0.06 0.05 – – – -0.72 -0.26 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0.1 -0.5 -0.3 0 0.4 0 0.5 0 0 0 – – –
Cytoplasm -1.3 -0.9 -3.5 -5.3 -5.4 -4.2 -6.0 -5.2 -7.2 1.3 1.5 1.7 – – –
Endoplasmic Reticulum 0 0 0 0 2.5 1.8 0 2.5 2.3 0 0 0 – – –
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 0 1.9 4.7 3.6 2.1 4.3 2.9 4.9 0 0 0 – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 18.5683 23.9293 22.5795 21.4202 19.1663 21.8579 17.998 21.9246 18.5828 18.5872 19.9689 18.3693 19.3157 23.5487 21.3904 20.5242 17.1181 21.2822
Actin 0.0234 0.0002 0.0031 0.0003 0.0142 0.0006 0.0223 0.0001 0.0049 0.0001 0.0068 0.0027 0.0028 0.0004 0.0032 0.0003 0.0049 0.0004
Bud 0.0014 0.0001 0.0012 0.0001 0.0005 0.0001 0.0001 0 0.0002 0 0.0001 0.0001 0.0002 0 0.0001 0.0001 0.0004 0
Bud Neck 0.0008 0.0002 0.0011 0.0004 0.0009 0.0009 0.0002 0.0001 0.0003 0.0003 0.0003 0.0006 0.0005 0.0001 0.0002 0.0003 0.0008 0.0004
Bud Periphery 0.0014 0 0.0015 0 0.0006 0 0.0001 0 0.0003 0 0 0.0001 0.0001 0 0 0.0001 0.0002 0
Bud Site 0.0036 0.0007 0.0042 0.0002 0.0018 0 0.0003 0 0.0003 0 0.0004 0 0.0007 0.0001 0.0002 0.0001 0.0016 0
Cell Periphery 0.0007 0.0001 0.0003 0.0001 0.0002 0 0 0 0.0001 0 0 0 0.0001 0 0 0 0.0001 0
Cytoplasm 0.8343 0.9744 0.935 0.9441 0.7411 0.944 0.8976 0.9951 0.9073 0.9841 0.9369 0.9544 0.915 0.9927 0.9608 0.9128 0.8981 0.9414
Cytoplasmic Foci 0.014 0.0008 0.006 0.0087 0.0403 0.0034 0.0024 0.0003 0.0095 0.0003 0.0349 0.0013 0.0023 0.0008 0.003 0.0054 0.0331 0.0014
Eisosomes 0.0004 0 0 0 0.0001 0 0.0003 0 0.0001 0 0 0 0.0001 0 0 0 0.0001 0
Endoplasmic Reticulum 0.0068 0.0003 0.0015 0.0022 0.0088 0.003 0.0134 0.0001 0.0024 0.0002 0.0018 0.0041 0.0042 0.0001 0.0001 0.0011 0.0014 0.0005
Endosome 0.0128 0.0003 0.006 0.0134 0.0442 0.0022 0.0095 0 0.0093 0.0001 0.0027 0.0055 0.004 0.0001 0.0011 0.003 0.0091 0.0116
Golgi 0.0075 0.0001 0.0021 0.0011 0.0282 0.0001 0.0015 0 0.0066 0 0.0009 0.0056 0.0033 0 0.002 0.0003 0.0039 0.003
Lipid Particles 0.0106 0 0.0024 0.0076 0.0151 0.0001 0.0007 0 0.0021 0 0.0011 0.0003 0.0061 0 0.0074 0.0057 0.0079 0.0106
Mitochondria 0.0092 0.0001 0.0079 0.0008 0.0127 0.0002 0.0009 0 0.0101 0.0001 0.0002 0.0014 0.005 0 0.0014 0.0013 0.015 0.0003
None 0.0033 0.0022 0.0007 0.0027 0.0072 0.0006 0.0043 0.0002 0.0006 0.0002 0.0017 0.0013 0.0012 0.0002 0.0002 0.0014 0.0008 0.0003
Nuclear Periphery 0.0144 0.0013 0.0029 0.002 0.0071 0.0063 0.0218 0.0002 0.0114 0.0014 0.0012 0.0031 0.0054 0.0005 0.0008 0.0185 0.0015 0.0035
Nucleolus 0.001 0 0.0002 0.0001 0.0003 0 0.0001 0 0.0135 0 0 0 0.0001 0 0 0.0039 0.0002 0
Nucleus 0.0358 0.0187 0.0187 0.0122 0.0248 0.0361 0.0208 0.0035 0.0157 0.0128 0.0084 0.0178 0.0451 0.0048 0.0107 0.0314 0.0128 0.0247
Peroxisomes 0.0084 0 0.0029 0.0003 0.0417 0.0001 0.0002 0 0.0019 0 0.0007 0 0.0019 0.0001 0.0084 0.0004 0.0057 0.0002
Punctate Nuclear 0.0067 0.0002 0.0005 0.0004 0.0033 0.0011 0.0016 0.0001 0.0015 0.0001 0.0015 0.0005 0.0007 0.0001 0.0003 0.0099 0.0011 0.0006
Vacuole 0.0026 0.0003 0.0013 0.0025 0.005 0.0008 0.001 0 0.0009 0.0001 0.0002 0.0007 0.0008 0.0001 0.0001 0.0016 0.0009 0.0004
Vacuole Periphery 0.001 0 0.0004 0.0006 0.0019 0.0002 0.001 0 0.0009 0.0001 0.0001 0.0004 0.0003 0 0 0.0024 0.0004 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 258.9623 262.81 274.6826 283.1757 264.3875 233.8122 245.0907 267.4953 262.1306 259.1259
Translational Efficiency 1.3972 1.3822 1.326 1.447 1.3434 1.5075 1.579 1.3845 1.45 1.4264

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2050 551 2444 1321 1252 2045 2032 749 3302 2596 4476 2070

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 2221.86 2314.19 2396.44 2471.58 1881.54 1925.24 2879.04 2294.55 2092.82 2007.79 2615.53 2407.52
Standard Deviation 480.22 404.49 458.78 532.78 511.32 420.85 572.16 474.68 519.20 446.70 566.81 519.53
Intensity Change Log 2 — 0.058739 0.109125 0.153666 — 0.033124 0.613674 0.286297 — 0.047051 0.362483 0.215999

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000087 0.000302 0.000051 0.000867 0.000211 0.000112 0.000497 0.000548 0.000134 0.000153 0.000254 0.000752
Bud Neck 0.000370 0.000945 0.000529 0.004951 0.000385 0.000287 0.000784 0.004491 0.000376 0.000426 0.000645 0.004784
Bud Site 0.000827 0.003628 0.001290 0.018249 0.001292 0.001709 0.002474 0.019426 0.001004 0.002116 0.001827 0.018675
Cell Periphery 0.000140 0.000303 0.000080 0.000372 0.000173 0.000053 0.000168 0.000319 0.000153 0.000106 0.000120 0.000353
Cytoplasm 0.791779* 0.734472* 0.752176* 0.637877* 0.783932* 0.829467* 0.781881* 0.681280* 0.788804* 0.809304* 0.765661* 0.653582*
Cytoplasmic Foci 0.002627 0.008540 0.001928 0.011574 0.003933 0.003357 0.002913 0.009867 0.003122 0.004457 0.002375 0.010957
Eisosomes 0.000001 0.000002 0.000001 0.000007 0.000001 0.000001 0.000001 0.000006 0.000001 0.000001 0.000001 0.000006
Endoplasmic Reticulum 0.000090 0.000378 0.000027 0.001278 0.000212 0.000104 0.000030 0.000216 0.000136 0.000162 0.000028 0.000894
Endosome 0.000066 0.000320 0.000207 0.008160 0.000140 0.000302 0.000354 0.008881 0.000094 0.000306 0.000274 0.008421
Golgi 0.000320 0.001571 0.000967 0.013782 0.000816 0.000479 0.001011 0.013188 0.000508 0.000710 0.000987 0.013567
Lipid Particles 0.000221 0.000693 0.000166 0.001972 0.000291 0.000140 0.000506 0.001017 0.000247 0.000257 0.000321 0.001627
Mitochondria 0.000102 0.000299 0.000178 0.001124 0.000122 0.000080 0.000337 0.002112 0.000109 0.000127 0.000250 0.001481
Mitotic Spindle 0.000312 0.001476 0.001957 0.064163 0.000781 0.002048 0.004257 0.032543 0.000490 0.001926 0.003001 0.052721
None 0.012612 0.021874 0.008455 0.008361 0.014782 0.014221 0.008187 0.008363 0.013435 0.015845 0.008333 0.008362
Nuclear Periphery 0.000067 0.000115 0.000090 0.000613 0.000105 0.000047 0.000121 0.000561 0.000082 0.000062 0.000104 0.000594
Nuclear Periphery Foci 0.000959 0.001964 0.000287 0.003251 0.002915 0.001004 0.000865 0.002741 0.001701 0.001208 0.000550 0.003066
Nucleolus 0.000076 0.000375 0.000085 0.000429 0.000080 0.000102 0.000122 0.000250 0.000077 0.000160 0.000102 0.000365
Nucleus 0.185355* 0.217687* 0.225684* 0.188576* 0.183385* 0.142544* 0.185097* 0.174467* 0.184608* 0.158493* 0.207258* 0.183471*
Peroxisomes 0.000018 0.000058 0.000027 0.000232 0.000010 0.000030 0.000022 0.000316 0.000015 0.000036 0.000025 0.000262
Vacuole 0.003639 0.004592 0.005631 0.024594 0.004942 0.003744 0.010164 0.030996 0.004133 0.003924 0.007689 0.026911
Vacuole Periphery 0.000331 0.000408 0.000185 0.009569 0.001492 0.000169 0.000207 0.008412 0.000771 0.000220 0.000195 0.009150

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.40 -0.08 -6.00 -0.34 -6.15 3.24 -0.50 -2.87 -5.64 -0.61 -0.91 -0.66 -6.54 -5.56 -2.89
Bud Neck -4.35 -5.58 -10.25 -6.24 -8.53 2.16 -9.56 -9.76 -10.21 -6.39 -1.88 -10.61 -13.75 -13.10 -10.37
Bud Site -3.00 -2.96 -7.17 -3.24 -6.34 -0.58 -2.64 -5.34 -5.23 -4.41 -2.93 -4.12 -8.91 -7.36 -7.55
Cell Periphery -3.95 0.31 -4.74 -0.08 -6.82 6.75 -2.18 -7.07 -12.47 -5.45 1.46 -0.88 -7.08 -10.27 -8.14
Cytoplasm 7.60 13.06 21.89 8.62 11.48 -7.41 7.03 13.66 19.85 9.27 -4.11 14.49 25.63 28.10 15.13
Cytoplasmic Foci -7.07 -2.04 -9.65 0.18 -8.71 1.71 -2.46 -10.39 -11.76 -9.32 -4.15 -3.73 -13.90 -10.61 -12.13
Eisosomes -4.12 -4.63 -11.11 -4.32 -9.74 2.35 -2.58 -10.46 -11.91 -8.30 -2.21 -4.90 -14.87 -13.04 -12.24
Endoplasmic Reticulum -1.19 3.35 -1.11 0.33 -1.52 3.06 5.95 3.49 0.20 -4.85 -0.25 6.67 -0.62 -0.45 -1.79
Endosome -2.40 -1.52 -4.26 -3.37 -3.92 -1.04 -0.62 -3.01 -2.74 -2.91 -1.93 -1.64 -5.15 -4.32 -4.83
Golgi -1.50 -0.98 -2.54 -0.85 -1.94 1.31 0.75 -1.53 -1.83 -1.73 -0.73 -0.45 -2.89 -2.62 -2.64
Lipid Particles -3.87 -3.22 -12.10 -6.40 -9.60 3.37 -4.10 -8.65 -10.05 -5.61 -0.37 -5.43 -14.85 -14.60 -10.73
Mitochondria -1.60 -4.51 -2.54 -0.22 -1.43 1.93 -0.95 -2.34 -2.60 -1.73 -0.51 -2.23 -3.43 -3.15 -2.18
Mitotic Spindle -2.49 -4.34 -6.48 -5.67 -5.30 -1.88 -1.93 -4.19 -3.66 -3.36 -3.23 -4.39 -7.69 -6.70 -6.27
None -2.93 6.45 8.28 5.47 3.96 0.70 6.16 6.96 9.57 2.46 -1.68 9.03 10.72 10.68 4.34
Nuclear Periphery -2.69 -10.64 -13.79 -8.41 -10.02 1.39 -2.64 -6.90 -10.79 -6.51 0.50 -6.95 -14.65 -16.63 -11.85
Nuclear Periphery Foci -4.54 3.75 -5.86 0.39 -8.74 5.06 4.64 1.12 -7.43 -6.26 2.50 5.41 -2.70 -6.39 -10.17
Nucleolus -1.51 -2.47 -5.00 0.42 -4.39 -2.14 -4.02 -4.31 -2.85 -1.52 -2.00 -4.67 -6.07 -1.70 -4.50
Nucleus -4.39 -11.52 -1.30 3.30 8.81 7.09 -2.66 1.91 -4.13 4.50 6.31 -10.12 0.03 -5.54 8.94
Peroxisomes -1.49 -0.59 -3.71 -0.87 -3.21 -2.44 -3.44 -3.20 -2.41 -2.78 -2.22 -1.27 -4.92 -3.20 -4.33
Vacuole -3.31 -8.90 -22.93 -21.12 -20.64 -0.35 -17.47 -17.11 -17.08 -11.46 -2.64 -19.26 -28.60 -28.02 -22.50
Vacuole Periphery -0.24 0.91 -2.05 -1.96 -2.39 1.52 1.56 -0.33 -2.36 -2.41 1.57 1.78 -1.69 -3.14 -3.29
Standard name
Human Ortholog
Description Spermidine synthase; involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells
Localization
Cell Percentages cytoplasm (96%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-7

Spe3

Spe3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spe3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available