Standard name
Description Spermidine synthase; involved in biosynthesis of spermidine and also in biosynthesis of pantothenic acid; spermidine is required for growth of wild-type cells

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.98 0.96 0.97 0.89 0.81 0.81 0.86 0.78 0.82 0.72 1.0 1.0 1.0 0.93 0.95 0.95 0.76 0.86 0.87
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0 0 0.06 0.05 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0.05 0.15 0.11 0.06 0.14 0.08 0.17 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 2 0 0 2 1 2 5 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 2 1 1 1 0 1 0 0 0 0 0 0 1 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 6 1 3 9 7 9 4 11 8 14 0 0 1 0 0 0 0 1 1
Cytoplasm 156 170 115 250 221 211 248 205 207 186 225 96 125 156 48 150 152 51 178 200
Endoplasmic Reticulum 0 2 4 2 7 8 18 10 7 14 17 0 0 0 0 0 0 2 0 2
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1
Golgi 0 0 0 0 0 2 5 0 4 1 8 0 0 0 0 0 0 0 0 1
Mitochondria 1 0 0 0 0 0 1 4 2 3 0 0 0 0 0 0 1 0 1 1
Nucleus 1 1 1 0 4 1 10 4 5 2 10 0 0 0 0 3 2 3 8 4
Nuclear Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 1 1 1
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 3 2 7 13 40 33 14 37 19 52 0 0 0 1 1 0 2 5 2
Unique Cell Count 159 173 120 258 247 259 306 237 266 228 314 96 125 156 52 158 161 67 208 229
Labelled Cell Count 160 182 123 262 256 271 325 245 275 235 333 96 125 157 52 158 161 67 208 229


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 28.4 28.6 29.6 29.4 23.9 22.9 21.4 19.3 20.1 19.4 20.9 31.7 29.9 29.6 17.3 23.9 24.7
Std Deviation (1e-4) 6.0 6.5 6.4 5.4 5.1 5.0 4.9 4.5 4.3 4.8 5.4 6.9 6.2 6.7 7.8 9.2 9.3
Intensity Change (Log2) 0.05 0.04 -0.26 -0.32 -0.42 -0.57 -0.51 -0.56 -0.46 0.15 0.06 0.05 -0.72 -0.26 -0.22


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.1 -0.5 -0.3 0 0.4 0 0.5 0 0 0
Cytoplasm -1.3 -0.9 -3.5 -5.3 -5.4 -4.2 -6.0 -5.2 -7.2 1.3 1.5 1.7
Endoplasmic Reticulum 0 0 0 0 2.5 1.8 0 2.5 2.3 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 1.9 4.7 3.6 2.1 4.3 2.9 4.9 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Spe3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available