Standard name
Human Ortholog
Description Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.07 0 0.08 0.09 0.12 0.1 0.08 0.05 0 0.07 0.09 0.12 0.05 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.07 0 0 0 0 0 0 0.07 0.14 0.23 0.18 0 0 0 0 0.05 0 0 0 0 0 0 0
Nucleus 0.84 0.92 0.9 0.93 0.89 0.83 0.76 0.71 0.71 0.66 0.74 0.87 0.82 0.78 0.84 0.84 0.86 0.81 0.85 0.68 0.75 0.71 0.71
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.35 0.15 0.14 0.1 0.05 0.12 0.15 0.26 0.31 0.39 0.32 0.09 0.13 0.11 0.14 0.12 0.13 0.14 0.08 0.16 0.11 0.16 0.14
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 1 0 0 0 1 0 1 0 0 0 3 0 0 0 0 0 1 1 2 1 1 1 0
Bud Neck 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 2 1 0 0 1 1 4 4 1 10 14 0 0 0 0 0 0
Cell Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 1 5 4 16 22 29 16 12 11 9 11 12 21 7 4 6 0 0 0 0 1 2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 3 0 0 0 0 1 1
Endosome 0 0 1 0 0 6 0 0 0 0 0 2 1 1 2 1 0 1 0 2 0 1 0
Golgi 0 0 0 0 0 1 0 0 0 0 0 0 1 1 0 3 1 0 1 0 0 0 0
Mitochondria 12 0 1 2 0 8 8 11 22 53 39 1 2 0 5 9 5 0 0 1 0 0 0
Nucleus 147 159 62 135 188 206 187 117 110 150 163 139 112 131 122 150 149 147 160 64 45 96 93
Nuclear Periphery 5 1 0 1 0 1 3 1 0 1 1 1 0 2 0 3 0 0 0 0 0 0 0
Nucleolus 62 25 10 15 11 29 38 43 49 89 70 15 18 19 21 22 23 25 15 15 6 21 18
Peroxisomes 0 0 0 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 1 0 1 1 2 1 0 0 3 0 2 0 0 1 2 1 1 0 2 2 3
Vac/Vac Membrane 0 2 0 0 2 5 2 0 0 1 0 2 0 2 2 5 6 4 4 1 1 6 8
Unique Cell Count 176 172 69 145 212 248 247 164 156 228 220 159 136 169 146 178 174 183 188 94 61 135 131
Labelled Cell Count 230 190 80 158 221 281 274 194 194 315 302 171 149 177 161 199 196 183 188 94 61 135 131


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 9.4 8.3 8.0 8.4 8.0 7.2 6.4 6.2 5.8 5.5 8.9 9.1 9.0 10.5 10.4 9.4 6.3 8.9 8.9
Std Deviation (1e-4) 1.4 1.8 2.0 1.8 1.9 4.0 2.0 2.2 2.2 1.8 1.4 1.7 2.5 2.0 2.5 2.7 2.5 1.1 2.1 1.7
Intensity Change (Log2) -0.07 0.01 -0.06 -0.22 -0.38 -0.43 -0.53 -0.6 0.09 0.12 0.11 0.33 0.32 0.17 -0.4 0.1 0.08


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.1 0.4 1.1 0.6 0.1 0 0 0 0.4 1.2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.9 4.1 3.4 0 0 0 0 0 0
Nucleus 0.8 -0.3 -1.4 -2.5 -3.1 -3.2 -3.9 -2.8 -0.5 -1.4 -2.2 -1.2 -1.1 -0.9
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.9 -2.6 -0.6 0.2 1.9 2.7 3.8 2.8 -1.1 -0.2 -0.7 0 -0.4 -0.3
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.5721 3.356 3.2691 3.3265 2.8791 3.3094 2.1935 3.2474 2.4022 2.1589 2.334 2.3466 2.8806 3.5568 2.7513 2.9002 2.3121 2.6526
Actin 0.0078 0 0.0007 0.0051 0 0 0.0015 0 0.0033 0.0349 0.0678 0.0004 0.0114 0 0.0198 0 0.0033 0
Bud 0.0004 0 0.0002 0.0004 0.0001 0 0.0002 0 0.0005 0.0007 0.003 0 0.0003 0 0.0001 0.0001 0.0005 0
Bud Neck 0.0008 0.0001 0.0006 0.0007 0.0012 0.0006 0.0004 0.0001 0.0002 0.0008 0.0016 0.001 0.0006 0.0001 0.0002 0.0002 0.0036 0.0008
Bud Periphery 0.0009 0 0.0007 0.0005 0.0003 0 0.0006 0 0.0006 0.0023 0.0041 0.0001 0.0005 0 0.0001 0.0001 0.0014 0.0001
Bud Site 0.0015 0 0.0007 0.0009 0.0002 0 0.0005 0 0.0042 0.0014 0.0117 0.0001 0.0013 0 0.0003 0.0001 0.0013 0.0001
Cell Periphery 0.0003 0 0.0005 0.0001 0.0001 0 0.0001 0 0.0001 0.0006 0.0003 0 0.0002 0 0 0 0.0003 0
Cytoplasm 0.0013 0.0001 0.0001 0.0007 0.0002 0 0.0083 0.0008 0.001 0.0008 0.002 0.0002 0.0005 0 0.0004 0 0.0012 0
Cytoplasmic Foci 0.0046 0 0.0015 0.002 0 0 0.0108 0 0.0122 0.0024 0.0089 0.0018 0.007 0 0.0015 0 0.0024 0
Eisosomes 0.0002 0 0 0 0 0 0.0001 0 0.0001 0.0002 0.0002 0 0.0001 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0008 0 0.0002 0.0015 0.0009 0 0.0014 0.0001 0.0014 0.0014 0.0044 0.0002 0.0005 0 0.0003 0 0.0011 0
Endosome 0.0095 0 0.0055 0.0209 0.0021 0 0.0136 0.0003 0.0096 0.0303 0.039 0.0032 0.0052 0 0.0024 0 0.0059 0
Golgi 0.0025 0 0.0023 0.005 0 0 0.0019 0 0.0032 0.0045 0.0092 0.0009 0.0037 0 0.0016 0 0.0024 0
Lipid Particles 0.0106 0 0.006 0.0019 0.0002 0 0.0139 0 0.0051 0.0036 0.0733 0.0006 0.0073 0 0.0074 0 0.0083 0
Mitochondria 0.0094 0 0.0076 0.0026 0.0007 0 0.0177 0 0.0041 0.0095 0.0514 0.0004 0.0053 0.0001 0.0006 0.0001 0.0115 0.0002
None 0.0042 0 0.0002 0.0006 0.0001 0 0.0032 0.0165 0.0013 0.0009 0.0009 0.0001 0.0006 0 0.0003 0 0.0006 0
Nuclear Periphery 0.008 0.0048 0.0031 0.0065 0.008 0.0002 0.0125 0.0023 0.0038 0.0148 0.01 0.0003 0.0054 0.0003 0.0015 0.0012 0.0091 0.0011
Nucleolus 0.0633 0.0504 0.0397 0.1033 0.2319 0.1386 0.0387 0.0407 0.0288 0.0474 0.1191 0.0724 0.0486 0.0413 0.0242 0.026 0.1866 0.1033
Nucleus 0.8472 0.9394 0.9193 0.8206 0.7288 0.8584 0.8491 0.9365 0.9036 0.8023 0.5534 0.9168 0.8858 0.9573 0.9361 0.9713 0.7295 0.8932
Peroxisomes 0.0072 0 0.0017 0.0004 0 0 0.003 0 0.0132 0.0072 0.0103 0.0001 0.0072 0 0.0006 0 0.0018 0
Punctate Nuclear 0.0151 0.005 0.0065 0.0234 0.0118 0.002 0.0181 0.0024 0.0029 0.0246 0.0189 0.001 0.0063 0.0008 0.0021 0.0008 0.0257 0.0009
Vacuole 0.0031 0 0.0018 0.0015 0.0091 0 0.0028 0.0003 0.0005 0.0058 0.0075 0.0002 0.0015 0 0.0002 0 0.0022 0.0001
Vacuole Periphery 0.0015 0 0.0012 0.0013 0.0042 0 0.0018 0.0001 0.0004 0.0035 0.0031 0.0001 0.0008 0 0.0002 0 0.0014 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.0276 8.6982 9.0362 16.4636 11.3541 5.8349 12.899 15.4332 12.807 17.8298
Translational Efficiency 0.9145 1.1023 0.9041 0.4804 0.6778 1.5917 0.7112 0.5618 0.7951 0.6411

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2039 1130 128 951 1289 1747 161 654 3328 2877 289 1605

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 777.69 903.44 1231.36 1170.34 841.23 896.14 1273.82 1102.50 802.30 899.01 1255.01 1142.70
Standard Deviation 100.60 158.65 175.60 174.26 124.46 150.84 149.71 188.24 114.71 154.00 163.06 183.15
Intensity Change Log 2 0.216234 0.662986 0.589661 0.091224 0.598589 0.390206 0.152629 0.629883 0.489464

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000222 0.000690 0.002004 0.002126 0.000465 0.000631 0.002176 0.001381 0.000316 0.000654 0.002100 0.001822
Bud Neck 0.003584 0.007458 0.014135 0.035247 0.004929 0.012615 0.014317 0.025834 0.004105 0.010589 0.014237 0.031411
Bud Site 0.000412 0.000690 0.002289 0.006018 0.000372 0.001450 0.002046 0.005016 0.000397 0.001152 0.002154 0.005610
Cell Periphery 0.000046 0.000050 0.000092 0.000286 0.000027 0.000068 0.000057 0.000167 0.000039 0.000061 0.000072 0.000238
Cytoplasm 0.000816 0.000800 0.000268 0.003825 0.000592 0.001178 0.000327 0.019914 0.000729 0.001029 0.000301 0.010381
Cytoplasmic Foci 0.000221 0.000678 0.000198 0.000723 0.000162 0.001033 0.000076 0.003020 0.000198 0.000893 0.000130 0.001659
Eisosomes 0.000019 0.000033 0.000085 0.000042 0.000030 0.000034 0.000083 0.000039 0.000023 0.000033 0.000084 0.000041
Endoplasmic Reticulum 0.001681 0.002137 0.002332 0.003376 0.001975 0.002658 0.004723 0.004716 0.001795 0.002453 0.003664 0.003922
Endosome 0.000131 0.000524 0.000116 0.002438 0.000078 0.000765 0.000144 0.003967 0.000110 0.000670 0.000131 0.003061
Golgi 0.000081 0.000613 0.000284 0.002446 0.000098 0.000759 0.000233 0.002290 0.000088 0.000702 0.000255 0.002382
Lipid Particles 0.000074 0.000285 0.000105 0.000147 0.000046 0.000507 0.000153 0.004405 0.000063 0.000420 0.000132 0.001882
Mitochondria 0.000583 0.001751 0.002360 0.010545 0.000805 0.003125 0.002247 0.007953 0.000669 0.002585 0.002297 0.009489
Mitotic Spindle 0.001784 0.003526 0.001275 0.029194 0.001117 0.005190 0.013656 0.037984 0.001525 0.004536 0.008172 0.032776
None 0.003529 0.003565 0.002073 0.004171 0.001979 0.002137 0.001643 0.014585 0.002928 0.002698 0.001833 0.008414
Nuclear Periphery 0.000197 0.000324 0.000182 0.002705 0.000109 0.000495 0.000215 0.001639 0.000163 0.000428 0.000200 0.002270
Nuclear Periphery Foci 0.000562 0.000624 0.000141 0.001986 0.000194 0.001104 0.000216 0.000947 0.000419 0.000915 0.000183 0.001563
Nucleolus 0.089351 0.109774 0.089169 0.046174 0.112725 0.100773 0.079728 0.034982 0.098404 0.104308 0.083910 0.041613
Nucleus 0.896200* 0.864733* 0.876672* 0.837642* 0.873373* 0.862898* 0.873467* 0.813865* 0.887359* 0.863619* 0.874887* 0.827954*
Peroxisomes 0.000263 0.001234 0.004927 0.000634 0.000662 0.000702 0.002712 0.000636 0.000417 0.000911 0.003693 0.000635
Vacuole 0.000135 0.000290 0.001027 0.005726 0.000132 0.000908 0.001528 0.009870 0.000134 0.000665 0.001306 0.007414
Vacuole Periphery 0.000109 0.000224 0.000270 0.004551 0.000130 0.000971 0.000253 0.006791 0.000117 0.000678 0.000260 0.005464

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -10.79 -12.36 -8.47 -6.42 0.12 -2.97 -4.70 -15.71 -13.65 1.32 -9.17 -7.61 -13.07 -10.54 1.24
Bud Neck -6.80 -8.15 -19.92 -17.90 -10.48 -11.83 -6.30 -15.83 -12.14 -8.25 -14.00 -10.07 -25.42 -20.82 -13.31
Bud Site -1.91 -6.16 -9.70 -9.19 -3.77 -2.80 -8.25 -8.48 -6.74 -5.02 -3.11 -9.96 -12.85 -10.46 -6.37
Cell Periphery -0.37 -2.29 -2.89 -2.82 -1.71 -2.28 -5.60 -12.65 -4.74 -7.17 -1.88 -4.09 -4.70 -3.76 -3.02
Cytoplasm 0.17 1.98 -3.86 -3.98 -4.70 -1.20 0.47 -4.50 -4.09 -4.65 -1.03 1.57 -5.82 -5.26 -6.30
Cytoplasmic Foci -1.30 -0.52 -1.02 0.65 -0.70 -1.64 0.75 -1.75 -0.04 -2.66 -2.20 0.54 -2.04 0.62 -2.80
Eisosomes -8.81 -10.70 -15.95 -8.79 5.21 -2.54 -10.76 -10.69 -8.55 5.38 -9.02 -15.63 -19.22 -12.05 7.64
Endoplasmic Reticulum -2.24 -3.34 -12.17 -8.50 -7.24 -3.89 -7.67 -10.36 -8.22 -2.31 -4.89 -8.17 -15.64 -11.43 -4.29
Endosome -1.81 -0.30 -3.07 -1.55 -3.05 -3.49 -3.07 -3.65 -1.94 -3.34 -3.83 -2.73 -4.73 -2.29 -4.46
Golgi -2.23 -6.02 -2.22 -0.94 -1.46 -2.67 -6.29 -3.14 -0.69 -2.32 -3.47 -8.67 -3.31 -1.06 -2.32
Lipid Particles -1.03 -2.16 -5.17 0.19 -2.90 -1.56 -3.05 -1.29 -0.89 -1.13 -1.82 -3.48 -1.49 -0.73 -1.23
Mitochondria -2.80 -13.81 -4.32 -3.30 -2.80 -3.54 -2.95 -4.34 -1.87 -1.88 -4.56 -5.22 -5.87 -3.42 -3.18
Mitotic Spindle -1.12 -1.50 -7.48 -7.01 -6.21 -2.91 -1.84 -7.52 -6.77 -2.81 -2.88 -2.09 -10.57 -9.63 -4.78
None -0.01 1.87 -1.47 -1.19 -3.55 -0.38 -0.11 -4.39 -4.52 -4.46 0.33 1.55 -4.48 -4.99 -5.73
Nuclear Periphery -0.94 -1.33 -7.92 -6.81 -7.74 -3.43 -4.59 -11.38 -8.04 -9.96 -3.02 -3.91 -11.78 -9.51 -11.03
Nuclear Periphery Foci -0.13 1.42 -0.91 -0.65 -2.25 -2.00 -0.70 -3.34 0.52 -1.84 -1.48 0.53 -1.87 0.04 -2.52
Nucleolus -4.54 -2.31 9.03 11.82 5.05 2.46 2.13 12.78 11.15 4.44 -2.00 -0.76 14.98 16.07 6.65
Nucleus 5.96 4.11 10.02 5.56 0.96 2.19 1.80 7.71 6.44 3.34 6.53 4.49 12.68 8.32 2.95
Peroxisomes -3.46 -7.74 -9.23 1.44 6.97 -0.36 -7.45 -2.56 -1.19 6.77 -3.77 -10.33 -8.80 0.55 9.20
Vacuole -1.37 -7.03 -7.22 -6.98 -5.60 -2.86 -4.58 -6.29 -5.76 -4.84 -3.17 -6.36 -9.23 -8.43 -6.82
Vacuole Periphery -3.57 -8.91 -2.19 -2.02 -1.87 -2.72 -4.62 -1.83 -1.05 -1.67 -2.99 -8.18 -2.83 -1.84 -2.50