ORF
Human Ortholog
Description Putative membrane protein; YPR071W is not an essential gene; YPR071W has a paralog, YIL029C, that arose from a single-locus duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.08 0.17 0 0 0 0 0 0 0 0 0.07 0.07 0 0.1 0.31 0.35 0.23 0.2 0.22 0.27 0.34 0.41
Bud 0 0 0 0.05 0 0.05 0 0.05 0.05 0 0.1 0 0.05 0 0 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0.06 0.05 0 0 0 0 0 0
Cytoplasm 0.17 0.2 0.44 0.35 0.4 0.52 0.54 0.54 0.54 0.5 0.5 0.23 0.44 0.5 0.37 0.29 0.3 0 0 0 0 0 0
Endoplasmic Reticulum 0.3 0.53 0.17 0 0 0 0 0 0 0 0 0.44 0.22 0.31 0 0 0.1 0.22 0.17 0.16 0.17 0.1 0.11
Endosome 0.19 0 0 0.16 0.17 0 0.11 0 0 0 0 0.19 0.16 0.11 0.13 0.14 0 0.08 0.07 0.07 0.05 0.05 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.05 0.11 0.06 0.05 0.05 0.08 0.08
Mitochondria 0.24 0.37 0 0.29 0.15 0.47 0.23 0.46 0.36 0.46 0.44 0.08 0.06 0 0.13 0.14 0.1 0.21 0.34 0.28 0.26 0.16 0.11
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.07 0 0 0.12 0.09 0 0 0 0 0 0 0 0.08 0 0 0 0 0.09 0.05 0.12 0.12 0.21 0.15
SpindlePole 0 0 0.14 0.08 0.12 0 0.1 0 0.06 0 0 0 0.05 0 0.1 0.14 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0.14 0 0.07 0.09 0.14 0 0.09 0.06 0.05 0 0 0.07 0 0 0.13 0.06 0.05 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 9 32 10 7 4 1 2 0 1 2 11 16 10 3 11 7 92 35 84 40 111 114
Bud 7 3 6 17 19 17 14 17 23 7 18 2 10 2 1 0 1 1 0 0 1 1 0
Bud Neck 0 0 4 1 2 0 1 3 3 1 1 0 0 0 0 0 0 5 1 6 1 1 3
Bud Site 0 0 0 0 0 2 0 1 2 1 2 0 0 0 0 0 0
Cell Periphery 4 0 0 6 4 0 2 3 2 0 1 3 3 11 2 2 1 0 2 7 2 8 11
Cytoplasm 48 24 84 120 181 168 260 192 237 82 91 35 95 128 11 10 6 1 2 8 2 2 4
Endoplasmic Reticulum 83 63 33 7 10 9 6 2 3 0 0 67 48 80 0 1 2 90 30 60 25 31 30
Endosome 53 2 8 56 76 8 51 4 12 5 4 29 34 27 4 5 0 33 12 27 6 18 13
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 3 1 46 10 19 7 25 21
Mitochondria 67 44 7 98 67 150 113 164 158 75 81 12 12 4 4 5 2 85 60 106 38 52 31
Nucleus 1 1 0 0 0 2 1 1 3 0 2 1 0 1 0 0 0 0 0 0 0 0 0
Nuclear Periphery 2 0 0 0 0 0 0 1 1 0 1 0 0 0 0 0 0 2 2 2 0 1 0
Nucleolus 0 0 0 0 0 2 1 1 0 2 1 0 1 0 1 0 0 0 0 0 0 0 0
Peroxisomes 20 0 2 40 42 2 12 2 8 2 5 6 17 7 0 0 0 37 8 45 18 70 41
SpindlePole 5 0 26 26 52 10 47 13 26 7 7 4 11 7 3 5 2 1 1 0 1 0 1
Vac/Vac Membrane 38 2 13 29 63 12 44 21 20 6 5 10 5 7 4 2 1 9 6 6 2 2 2
Unique Cell Count 279 118 189 341 451 322 484 353 440 163 183 152 214 255 30 35 20 411 179 382 151 329 280
Labelled Cell Count 338 148 215 411 523 386 553 427 498 189 221 180 252 284 33 44 23 411 179 382 151 329 280


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.2 4.8 5.9 5.6 6.1 4.3 4.9 4.1 4.3 4.2 4.2 6.2 5.6 6.1 6.7 6.9 7.3 5.2 5.3 5.9
Std Deviation (1e-4) 0.7 0.7 1.4 1.4 1.8 1.2 1.1 0.9 1.1 1.5 1.0 1.4 1.6 1.4 1.9 0.9 1.7 1.2 0.8 1.9
Intensity Change (Log2) -0.06 0.04 -0.47 -0.28 -0.52 -0.44 -0.48 -0.48 0.06 -0.06 0.04 0.18 0.23 0.31 -0.19 -0.15 0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.5081 0.1902 -0.2798 -0.57 -0.4002 -0.3535 1.9041 1.7796 1.6794 1.0343 1.716 1.6341 3.1614 4.4367 4.044 3.7311 4.0058 3.8482
Actin 0.0875 0.0519 0.0829 0.0687 0.0914 0.0532 0.0842 0.0622 0.0665 0.0803 0.0525 0.0506 0.0868 0.1077 0.0874 0.0934 0.1367 0.067
Bud 0.0011 0.0006 0.0011 0.0003 0.0008 0.0008 0.0005 0.0008 0.0012 0.0006 0.0018 0.0004 0.0002 0.0012 0.0003 0.0004 0.0002 0.0002
Bud Neck 0.0027 0.0017 0.0051 0.0311 0.0018 0.0078 0.0025 0.0023 0.0122 0.0152 0.003 0.0078 0.0015 0.0017 0.0032 0.0258 0.0026 0.0073
Bud Periphery 0.0011 0.0007 0.0013 0.0005 0.001 0.0011 0.0008 0.0017 0.0049 0.0011 0.0027 0.0007 0.0004 0.0014 0.0005 0.0008 0.0003 0.0005
Bud Site 0.0094 0.007 0.0071 0.0012 0.0088 0.001 0.0057 0.0197 0.0132 0.0015 0.0031 0.0012 0.0021 0.0152 0.0058 0.0069 0.0009 0.0005
Cell Periphery 0.0029 0.0025 0.0017 0.0007 0.0005 0.0013 0.0017 0.003 0.004 0.001 0.0015 0.0015 0.0033 0.0018 0.0028 0.0027 0.001 0.0024
Cytoplasm 0.0989 0.1132 0.0755 0.0621 0.0748 0.1053 0.1163 0.1598 0.142 0.154 0.0579 0.1381 0.1079 0.1921 0.1114 0.1198 0.1083 0.1412
Cytoplasmic Foci 0.1044 0.1331 0.1147 0.1252 0.1087 0.1218 0.1077 0.1312 0.1037 0.1136 0.1073 0.1104 0.1197 0.1129 0.1016 0.1552 0.1524 0.0945
Eisosomes 0.0023 0.0007 0.0015 0.0009 0.0006 0.0007 0.001 0.0013 0.0013 0.0006 0.0009 0.0006 0.0025 0.0007 0.0022 0.0021 0.0011 0.0017
Endoplasmic Reticulum 0.1623 0.1156 0.1403 0.1011 0.0528 0.1258 0.1635 0.1905 0.2101 0.1272 0.1574 0.1927 0.2092 0.1586 0.2138 0.1708 0.0735 0.2313
Endosome 0.141 0.1699 0.1622 0.1691 0.1418 0.1557 0.1287 0.1215 0.1314 0.1769 0.146 0.1436 0.0922 0.0924 0.1007 0.0741 0.0855 0.0808
Golgi 0.0702 0.1358 0.0827 0.0723 0.1509 0.0664 0.0567 0.0594 0.0643 0.0688 0.0722 0.0636 0.0517 0.0751 0.0601 0.0516 0.0548 0.0512
Lipid Particles 0.1479 0.1343 0.1447 0.197 0.2399 0.1621 0.1373 0.1331 0.1054 0.0558 0.1307 0.1449 0.2167 0.1257 0.1546 0.0985 0.0965 0.1618
Mitochondria 0.0195 0.0094 0.0218 0.0187 0.043 0.0269 0.0106 0.0141 0.0208 0.0244 0.1363 0.0167 0.0043 0.0047 0.0079 0.0077 0.004 0.0103
None 0.0743 0.0487 0.0729 0.07 0.0046 0.0771 0.0903 0.0534 0.0574 0.1134 0.0489 0.0474 0.0662 0.0808 0.1023 0.1212 0.2186 0.0949
Nuclear Periphery 0.0294 0.0361 0.0178 0.0239 0.017 0.0337 0.0381 0.0172 0.0153 0.0224 0.0171 0.0278 0.0132 0.0065 0.0109 0.0084 0.0079 0.0202
Nucleolus 0.0023 0.0004 0.0019 0.0003 0.0006 0.0005 0.0008 0.0018 0.0006 0.0002 0.0006 0.0004 0.0002 0.0002 0.0002 0.0003 0.0003 0.0002
Nucleus 0.007 0.0026 0.0089 0.0022 0.0009 0.0036 0.0099 0.0018 0.0025 0.0018 0.0063 0.0058 0.001 0.0014 0.0011 0.0012 0.0011 0.0015
Peroxisomes 0.0169 0.0233 0.0334 0.0333 0.0498 0.033 0.0233 0.0118 0.026 0.022 0.039 0.0298 0.0123 0.0124 0.0243 0.0507 0.0483 0.0219
Punctate Nuclear 0.0085 0.0043 0.0124 0.0116 0.0042 0.0104 0.012 0.0034 0.0068 0.0071 0.0033 0.0066 0.0042 0.003 0.0032 0.0044 0.0038 0.005
Vacuole 0.0073 0.0058 0.007 0.0056 0.0035 0.0075 0.0055 0.0076 0.0081 0.0091 0.0074 0.0067 0.0035 0.0036 0.0044 0.003 0.0019 0.0038
Vacuole Periphery 0.0031 0.0024 0.003 0.0041 0.0025 0.0044 0.0029 0.0022 0.0024 0.003 0.0041 0.0028 0.0012 0.0009 0.0014 0.0011 0.0007 0.0017

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.7468 3.1018 5.7542 13.5604 11.247 5.2019 7.4486 9.5254 7.7345 12.9131
Translational Efficiency 0.6788 2.0163 1.2015 0.5689 0.566 1.641 0.9655 0.5296 0.9402 0.6763

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
723 1325 1373 2081 2230 2286 154 1309 2953 3611 1527 3390

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 649.13 792.70 874.29 911.44 714.81 824.62 1073.96 925.17 698.73 812.91 894.43 916.74
Standard Deviation 85.23 109.77 101.47 125.66 84.89 100.65 99.53 154.96 89.55 105.22 117.78 137.87
Intensity Change Log 2 0.288268 0.429604 0.489640 0.206170 0.587309 0.372159 0.245825 0.514399 0.429265

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.034269 0.027895 0.018117 0.022101 0.006204 0.019613 0.028444 0.023349 0.013076 0.022652 0.019158 0.022583
Bud Neck 0.003354 0.001739 0.003728 0.001505 0.003214 0.002747 0.001658 0.001817 0.003249 0.002377 0.003520 0.001625
Bud Site 0.002422 0.004863 0.013261 0.006687 0.002591 0.004593 0.008837 0.008833 0.002550 0.004692 0.012815 0.007516
Cell Periphery 0.004607 0.001444 0.000558 0.000567 0.000561 0.000705 0.001693 0.000854 0.001551 0.000976 0.000673 0.000678
Cytoplasm 0.081256 0.056057 0.065827 0.030731 0.143758* 0.083593 0.021810 0.031127 0.128456* 0.073489 0.061387 0.030884
Cytoplasmic Foci 0.143580 0.124670 0.051667 0.039049 0.167037 0.105236 0.026790 0.044898 0.161294 0.112367 0.049158 0.041308
Eisosomes 0.020713 0.004753 0.000312 0.000743 0.003094 0.004332 0.000955 0.001069 0.007408 0.004487 0.000377 0.000869
Endoplasmic Reticulum 0.011258 0.078416 0.156349* 0.184537* 0.053562 0.071952 0.382115* 0.315560* 0.043204 0.074324 0.179118* 0.235129*
Endosome 0.009552 0.030198 0.027078 0.041893 0.013472 0.018987 0.035557 0.054431 0.012512 0.023100 0.027933 0.046734
Golgi 0.129841* 0.239141* 0.015654 0.050902 0.099991 0.164463* 0.020879 0.054335 0.107299 0.191865* 0.016181 0.052228
Lipid Particles 0.240797* 0.331229* 0.320363* 0.506562* 0.382636* 0.408666* 0.266346* 0.356315* 0.347909* 0.380251* 0.314915* 0.448546*
Mitochondria 0.039984 0.022496 0.002622 0.002462 0.022128 0.024392 0.000267 0.002206 0.026500 0.023696 0.002384 0.002363
Mitotic Spindle 0.001375 0.001464 0.029580 0.014725 0.002070 0.004541 0.004692 0.011627 0.001900 0.003412 0.027070 0.013529
None 0.003568 0.001760 0.001142 0.003311 0.002887 0.002393 0.000126 0.000755 0.003054 0.002161 0.001040 0.002324
Nuclear Periphery 0.000310 0.000413 0.023280 0.003373 0.001872 0.000894 0.004530 0.001818 0.001490 0.000718 0.021389 0.002773
Nuclear Periphery Foci 0.001609 0.004639 0.214864* 0.067029 0.005420 0.005349 0.167262 0.066251 0.004487 0.005089 0.210064* 0.066729
Nucleolus 0.000598 0.000358 0.001064 0.000154 0.000546 0.000424 0.000176 0.000103 0.000559 0.000400 0.000975 0.000134
Nucleus 0.015533 0.004929 0.028134 0.002758 0.029642 0.010607 0.004823 0.002717 0.026188 0.008524 0.025783 0.002742
Peroxisomes 0.251114* 0.056998 0.012836 0.010777 0.051677 0.059873 0.007670 0.006765 0.100506* 0.058818 0.012315 0.009228
Vacuole 0.003424 0.004144 0.013127 0.009273 0.006343 0.004857 0.015157 0.013190 0.005628 0.004595 0.013331 0.010785
Vacuole Periphery 0.000836 0.002396 0.000436 0.000861 0.001293 0.001783 0.000212 0.001980 0.001181 0.002008 0.000414 0.001293

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 1.65 4.36 3.21 2.05 -1.58 -8.50 -3.05 -7.57 -1.54 0.62 -6.33 -2.71 -5.53 -0.04 -1.61
Bud Neck 2.76 -0.72 2.93 0.36 3.59 0.90 2.76 3.38 1.86 -0.25 2.23 -0.75 4.74 2.11 3.47
Bud Site -2.83 -5.83 -4.48 -1.94 3.19 -2.50 -2.31 -5.56 -3.69 0.02 -3.48 -6.12 -6.70 -3.82 2.90
Cell Periphery 4.22 5.69 5.69 3.41 -0.02 -0.99 -2.12 -2.06 -1.48 1.57 2.54 4.03 4.19 2.55 -0.03
Cytoplasm 4.30 1.92 8.94 7.03 8.97 12.74 17.16 25.34 14.22 -1.31 14.53 13.82 27.70 16.31 8.83
Cytoplasmic Foci 2.66 13.49 16.09 19.09 4.33 12.78 25.43 27.18 15.59 -3.70 12.18 27.58 33.49 24.79 3.31
Eisosomes 6.71 8.80 8.61 7.54 -6.21 -2.82 4.98 6.24 8.22 -0.28 4.26 11.36 10.49 11.83 -5.33
Endoplasmic Reticulum -14.10 -22.31 -28.11 -14.59 -3.75 -4.50 -12.23 -27.90 -25.50 2.28 -9.50 -20.06 -34.82 -28.14 -7.33
Endosome -13.28 -11.61 -17.70 -5.46 -7.02 -5.10 -6.07 -16.94 -14.14 -4.30 -11.68 -11.66 -23.54 -15.12 -10.22
Golgi -14.08 19.96 13.40 33.47* -16.95 -14.24 19.43 12.38 25.70 -8.68 -21.35 33.63 18.97 41.60 -20.62
Lipid Particles -7.62 -6.07 -22.10 -16.23 -17.47 -2.95 5.52 2.67 5.15 -4.05 -4.50 4.24 -12.38 -8.60 -14.57
Mitochondria 4.32 10.51 10.57 10.16 0.49 -1.19 17.82 15.20 14.19 -4.16 1.59 18.50 18.54 17.58 0.20
Mitotic Spindle -0.09 -10.50 -7.67 -8.16 4.92 -2.48 -1.73 -5.37 -3.79 -2.47 -2.18 -10.54 -9.23 -7.80 5.18
None 1.15 1.60 0.17 -1.43 -2.33 0.80 6.79 4.81 3.56 -2.94 1.49 3.58 1.08 -0.23 -2.03
Nuclear Periphery -0.86 -12.59 -8.73 -8.46 10.59 4.31 -2.16 0.06 -2.83 2.14 4.55 -12.00 -4.69 -8.21 11.06
Nuclear Periphery Foci -3.43 -29.88* -19.98 -19.20 18.70 -0.01 -8.68 -15.69 -15.68 5.26 -1.06 -30.77 -24.89 -24.69 19.91
Nucleolus 0.77 -1.70 1.79 0.92 3.50 0.88 3.58 6.53 2.43 1.40 1.23 -2.02 5.43 2.30 3.59
Nucleus 3.99 -4.29 4.82 2.22 10.97 11.82 10.07 18.98 8.28 1.43 13.05 -0.92 18.83 8.44 11.06
Peroxisomes 20.75* 26.26 26.63* 16.71 1.32 -2.74 14.69 20.50 23.03 0.42 11.75 26.40 28.69 27.67 2.36
Vacuole -0.63 -8.79 -5.81 -6.07 2.04 2.17 -2.98 -5.07 -5.86 0.63 2.02 -8.88 -7.08 -8.13 1.76
Vacuole Periphery -3.44 2.83 -0.10 3.31 -2.42 -1.84 7.28 -1.10 -0.28 -2.94 -3.43 6.40 -0.38 2.20 -3.42