Standard name
Human Ortholog
Description Membrane protein; has similarity to mammalian mannose-6-phosphate receptors; possibly functions as a sorting receptor in the delivery of vacuolar hydrolases; protein abundance increases in response to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0.08 0.09 0.15 0.17 0.14 0.21 0.26 0.25 0.33 0 0 0 0.06 0.1 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0
Endosome 0.19 0.26 0.34 0.4 0.37 0.07 0.12 0 0 0.11 0 0 0.46 0.58 0.57 0.43 0.39 0.42 0.43 0.53 0.56 0.65 0.55 0.55
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.17 0.05 0.48 0.46 0.4 0.62 0.59 0.6 0.58 0.43 0.62 0.58 0 0.05 0 0.07 0.1 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0
Nucleolus 0.2 0.17 0.2 0.16 0.07 0 0 0 0 0 0 0 0.23 0.23 0.38 0.16 0.16 0.18 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.06 0.08 0 0 0 0 0 0 0 0 0.05 0.11 0.1 0.15 0.1 0.12 0 0 0 0 0 0
Vac/Vac Membrane 0.67 0.73 0.39 0.34 0.44 0.29 0.34 0.35 0.34 0.4 0.32 0.26 0.67 0.49 0.43 0.62 0.61 0.65 0.43 0.38 0.29 0.24 0.32 0.34
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 3 3 2 5 1 1 2 1 0 0 1 0 0 1 1 1 0 0 2
Bud 0 0 4 5 15 19 31 21 23 20 12 24 0 2 1 0 0 1 1 2 0 0 3 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
Bud Site 0 0 0 0 0 0 1 0 1 1 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 3 3 8 5 6 15 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 13 14 17 32 57 108 158 135 161 169 123 295 6 11 10 13 26 16 1 1 1 2 2 2
Endoplasmic Reticulum 6 1 1 0 4 35 10 19 28 16 21 27 3 8 5 6 8 17 1 1 4 0 1 2
Endosome 55 93 150 165 238 48 110 39 28 70 7 20 134 226 214 96 106 124 137 197 261 174 225 231
Golgi 2 0 0 4 5 10 1 0 1 0 0 1 2 8 11 3 8 5 2 6 3 2 8 5
Mitochondria 50 19 209 192 255 459 538 569 436 274 303 518 7 20 8 16 27 10 8 3 17 6 11 6
Nucleus 9 6 2 1 10 10 17 12 13 8 3 6 2 2 2 3 3 7 5 1 1 1 4 3
Nuclear Periphery 7 4 22 10 6 14 16 30 14 9 5 7 12 17 16 4 3 4 10 8 23 7 8 11
Nucleolus 57 61 88 65 43 18 20 34 17 6 1 3 67 91 143 35 43 53 5 3 2 2 1 5
Peroxisomes 0 0 0 1 2 3 1 0 0 0 0 0 0 5 0 1 1 0 1 0 0 0 0 0
SpindlePole 6 5 27 35 26 8 10 1 5 10 1 0 15 42 36 33 28 36 3 5 6 3 3 2
Vac/Vac Membrane 193 256 172 140 282 213 308 330 256 253 156 237 197 189 162 139 167 193 138 141 137 65 131 146
Unique Cell Count 287 352 437 415 640 738 914 943 753 638 492 900 293 388 374 224 272 297 322 376 469 270 408 425
Labelled Cell Count 399 459 692 650 943 948 1227 1195 996 842 639 1155 446 621 608 350 420 466 322 376 469 270 408 425


Endosome

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 9.5 6.0 5.9 5.8 5.5 5.2 5.4 5.6 5.4 5.4 5.4 9.4 9.6 11.4 9.5 9.5 9.2 9.9 10.1 11.1
Std Deviation (1e-4) 2.3 2.2 1.4 1.4 1.5 1.3 1.1 1.2 1.3 1.2 1.3 1.2 1.9 2.7 2.2 2.5 2.6 2.3 2.3 2.6 2.8
Intensity Change (Log2) -0.0 -0.04 -0.11 -0.19 -0.14 -0.08 -0.15 -0.14 -0.16 0.66 0.69 0.93 0.67 0.67 0.62 0.74 0.76 0.9


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 1.7 2.0 2.7 1.7 2.4 2.4 1.8 2.1 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.4 3.2 5.8 6.9 5.8 8.2 9.6 9.0 11.7 -1.4 -0.8 -1.0 1.1 3.1 1.0
Endoplasmic Reticulum 0 0 4.3 0 2.6 3.8 2.9 4.0 3.3 0 0 0 0 0 4.7
Endosome 1.6 1.0 -12.3 -9.7 -15.2 -14.3 -9.3 -13.4 -16.5 3.1 6.9 6.5 2.1 1.3 2.0
Golgi 0 0 0 0 0 0 0 0 0 0 0 3.6 0 0 0
Mitochondria -0.5 -2.6 4.8 3.8 4.4 3.4 -1.6 4.2 3.4 -13.2 -13.7 -14.7 -10.4 -10.4 -12.9
Nucleus 0 0 0 2.0 0 1.9 0 0 0 0 0 0 0 0 0
Nuclear Periphery -2.0 -4.1 -3.0 -3.4 -1.7 -3.1 -3.5 -3.6 -5.0 -0.6 -0.4 -0.5 -2.0 -2.8 -2.7
Nucleolus -1.7 -6.6 -10.2 -11.4 -10.1 -10.5 -10.9 -10.3 -13.5 0.9 1.2 5.7 -1.4 -1.4 -0.8
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1.3 -1.6 -5.0 -5.4 -7.4 -5.7 -4.1 -5.3 -7.5 -0.6 2.4 1.8 3.6 2.0 2.8
Vacuole -1.7 1.5 -3.7 -2.0 -1.6 -1.9 0.1 -2.4 -4.8 7.4 2.7 1.1 5.5 5.7 6.8

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.8995 3.9463 3.3866 2.8073 3.0153 3.6192 4.759 5.9481 5.5786 5.3638 5.5741 5.6921 2.7949 4.2528 3.1939 3.6216 3.0339 3.3898
Actin 0.0032 0.0001 0.0016 0.004 0.0003 0.0002 0.0152 0 0.0001 0.0005 0.0023 0.001 0.0033 0 0 0 0 0.0001
Bud 0.0002 0.0004 0.0002 0.0103 0.0005 0.0013 0.0001 0 0.0001 0.0001 0.0003 0.0001 0.0001 0 0 0 0 0.0001
Bud Neck 0.0006 0.0004 0.0006 0.0022 0.0006 0.0008 0.0003 0.0003 0.0003 0.0002 0.0004 0.0002 0.0003 0.0003 0.0002 0.0006 0.0002 0.0002
Bud Periphery 0.0002 0.0003 0.0002 0.0057 0.0005 0.0007 0.0002 0.0001 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0001 0 0.0001
Bud Site 0.0028 0.0007 0.0009 0.0041 0.0018 0.0003 0.0003 0.0002 0.0002 0.0003 0.0015 0.0002 0.0004 0.0002 0.0001 0.0002 0.0002 0.0001
Cell Periphery 0.0001 0.0001 0.0002 0.0004 0.0002 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001 0.0002 0.0002 0.0002 0.0002 0.0002 0.0001 0.0001
Cytoplasm 0.0027 0.0022 0.003 0.0037 0.001 0.0019 0.0016 0.0007 0.0006 0.001 0.0014 0.0004 0.0013 0.0006 0.0005 0.0005 0.0006 0.0007
Cytoplasmic Foci 0.1226 0.1562 0.1223 0.1078 0.1561 0.0944 0.0818 0.1108 0.0788 0.0536 0.1008 0.0619 0.0743 0.0869 0.0744 0.071 0.0858 0.0651
Eisosomes 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0006 0.0003 0.0016 0.0003 0.0006 0.0004 0.0012 0.0003 0.0003 0.0005 0.0007 0.0004 0.0007 0.0004 0.0004 0.0002 0.0002 0.0005
Endosome 0.5122 0.511 0.5011 0.6771 0.5454 0.5047 0.508 0.436 0.5514 0.6383 0.5051 0.4659 0.5074 0.4587 0.5494 0.6523 0.6803 0.4756
Golgi 0.0524 0.0509 0.0901 0.0439 0.1038 0.0763 0.0613 0.0827 0.0887 0.0822 0.0683 0.0763 0.0599 0.0499 0.0875 0.0556 0.0817 0.0864
Lipid Particles 0.1656 0.1645 0.1355 0.0685 0.1247 0.1983 0.2195 0.2096 0.1881 0.0607 0.188 0.2928 0.2285 0.2585 0.1737 0.0946 0.1008 0.2633
Mitochondria 0.0063 0.0018 0.0353 0.0035 0.0072 0.004 0.011 0.0015 0.0072 0.029 0.0342 0.0087 0.0053 0.0012 0.0073 0.0016 0.002 0.005
None 0.0039 0.0001 0.0007 0.0003 0.0001 0.0004 0.0008 0.0001 0.0001 0.0005 0.0005 0.0001 0.0009 0.0001 0.0001 0 0.0001 0.0001
Nuclear Periphery 0.0026 0.0037 0.002 0.0004 0.001 0.0026 0.0019 0.0059 0.0009 0.0008 0.0096 0.0012 0.0036 0.004 0.0013 0.001 0.0005 0.0049
Nucleolus 0.0079 0.0018 0.0042 0.0004 0.0012 0.0049 0.0011 0.001 0.0007 0.0003 0.0024 0.0013 0.0031 0.0009 0.0008 0.0014 0.0006 0.0019
Nucleus 0.0029 0.0005 0.0046 0.0003 0.0004 0.0024 0.0003 0.0003 0.0002 0.0002 0.0055 0.0003 0.0044 0.0003 0.0003 0.0003 0.0002 0.0003
Peroxisomes 0.0327 0.021 0.0276 0.015 0.0203 0.023 0.032 0.016 0.0175 0.0154 0.0207 0.0172 0.0181 0.0123 0.011 0.0036 0.0063 0.0132
Punctate Nuclear 0.0123 0.0017 0.0049 0.0009 0.0005 0.0022 0.0022 0.0014 0.0005 0.0012 0.028 0.0009 0.0014 0.0005 0.0004 0.0002 0.0005 0.0017
Vacuole 0.0467 0.0555 0.0437 0.036 0.0221 0.0515 0.0362 0.0809 0.0396 0.0704 0.0191 0.0384 0.052 0.0629 0.0517 0.0467 0.0242 0.0397
Vacuole Periphery 0.0215 0.0268 0.0195 0.0152 0.0114 0.0295 0.0249 0.0521 0.0244 0.0447 0.0107 0.0325 0.0347 0.0622 0.0409 0.0698 0.0158 0.0411

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 10.1097 9.4678 9.5803 14.8783 8.7385 9.623 16.0999 17.0337 15.0235 15.7662
Translational Efficiency 1.4438 1.5326 1.5877 0.9495 1.2181 1.6027 0.9451 1.0208 1.1937 1.1051

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
505 1362 97 1400 2042 1876 185 1993 2547 3238 282 3393

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 869.08 981.06 1271.88 1263.64 895.13 909.71 1128.44 1271.50 889.97 939.72 1177.78 1268.26
Standard Deviation 144.65 173.29 154.75 209.08 158.51 136.13 166.99 199.28 156.20 156.87 176.56 203.41
Intensity Change Log 2 0.174852 0.549402 0.540025 0.023309 0.334161 0.506362 0.099952 0.444204 0.523043

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000087 0.000106 0.000411 0.000638 0.000064 0.000045 0.000281 0.000228 0.000069 0.000071 0.000325 0.000398
Bud Neck 0.002565 0.001554 0.016746 0.004130 0.001147 0.001712 0.012064 0.001699 0.001428 0.001646 0.013674 0.002702
Bud Site 0.011425 0.006341 0.006266 0.009312 0.007559 0.003423 0.009633 0.006065 0.008326 0.004651 0.008475 0.007405
Cell Periphery 0.000452 0.000193 0.000193 0.000228 0.000251 0.000191 0.000117 0.000231 0.000291 0.000192 0.000143 0.000229
Cytoplasm 0.009348 0.001836 0.000717 0.002023 0.006102 0.001074 0.001333 0.001775 0.006745 0.001394 0.001121 0.001877
Cytoplasmic Foci 0.024511 0.037196 0.078010 0.078320 0.035651 0.027956 0.135914 0.061178 0.033442 0.031843 0.115997 0.068251
Eisosomes 0.000017 0.000013 0.000051 0.000034 0.000010 0.000011 0.000039 0.000021 0.000012 0.000012 0.000043 0.000026
Endoplasmic Reticulum 0.000707 0.000326 0.000979 0.000292 0.000366 0.000355 0.000484 0.000189 0.000434 0.000343 0.000654 0.000232
Endosome 0.315069* 0.464486* 0.169419 0.314188* 0.487264* 0.443446* 0.422992* 0.361932* 0.453123* 0.452296* 0.335770* 0.342232*
Golgi 0.050759 0.076931 0.059050 0.141336* 0.107907 0.065601 0.061134 0.158770* 0.096576 0.070367 0.060417 0.151577*
Lipid Particles 0.001594 0.005821 0.004746 0.013957 0.001547 0.003820 0.010167 0.007526 0.001557 0.004662 0.008302 0.010179
Mitochondria 0.014905 0.013409 0.030778 0.035263 0.011616 0.021169 0.009776 0.025913 0.012268 0.017905 0.017000 0.029771
Mitotic Spindle 0.001413 0.003339 0.023334 0.008303 0.000663 0.001738 0.017247 0.004221 0.000811 0.002412 0.019340 0.005906
None 0.000276 0.000132 0.000270 0.000184 0.000467 0.000160 0.000071 0.000115 0.000429 0.000149 0.000140 0.000144
Nuclear Periphery 0.000254 0.000593 0.016755 0.001422 0.000110 0.000898 0.001930 0.000818 0.000138 0.000770 0.007030 0.001067
Nuclear Periphery Foci 0.000751 0.003602 0.026620 0.005252 0.000771 0.004197 0.017394 0.002781 0.000767 0.003946 0.020568 0.003800
Nucleolus 0.000273 0.000286 0.018098 0.000936 0.000435 0.000531 0.005733 0.000340 0.000403 0.000428 0.009986 0.000586
Nucleus 0.000525 0.000237 0.005204 0.000250 0.000655 0.000375 0.002052 0.000121 0.000630 0.000317 0.003136 0.000174
Peroxisomes 0.003126 0.003361 0.017662 0.009463 0.001494 0.002931 0.042689 0.006077 0.001818 0.003112 0.034081 0.007474
Vacuole 0.278610* 0.237299* 0.321021* 0.242399* 0.193922 0.234139* 0.186256 0.225943* 0.210714* 0.235468* 0.232611* 0.232733*
Vacuole Periphery 0.283334* 0.142937* 0.203670* 0.132069* 0.141998* 0.186227* 0.062694 0.134056* 0.170021* 0.168018* 0.111186* 0.133236*

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -0.46 -3.12 -2.69 -2.59 -1.00 1.00 -2.52 -4.18 -5.06 0.90 -0.10 -3.72 -3.75 -3.74 -0.36
Bud Neck 1.86 -4.49 -2.67 -6.21 4.01 -1.25 -4.03 -2.54 0.00 3.84 -0.70 -5.88 -5.34 -3.59 5.27
Bud Site 2.73 2.56 0.91 -2.58 -2.29 4.61 -1.49 1.53 -3.16 2.27 4.49 -0.50 0.86 -4.00 1.05
Cell Periphery 2.25 2.22 1.94 -1.86 -1.41 1.11 3.69 0.52 -0.85 -5.03 2.26 3.94 1.61 -1.36 -5.22
Cytoplasm 2.84 3.29 2.66 -0.74 -3.25 5.30 4.96 4.41 -1.93 -1.05 5.78 6.09 5.03 -1.83 -2.74
Cytoplasmic Foci -4.16 -5.21 -13.48 -10.85 -0.05 3.94 -9.03 -9.26 -12.16 6.62 0.96 -10.00 -15.21 -16.20 5.67
Eisosomes 1.70 -4.04 -4.81 -8.39 2.04 -1.40 -4.96 -7.41 -6.39 3.14 -0.55 -6.66 -10.24 -10.50 3.54
Endoplasmic Reticulum 2.41 -0.70 2.41 0.45 1.85 0.29 -1.38 5.15 5.75 2.83 2.09 -1.68 4.03 3.18 3.02
Endosome -9.63 6.58 0.14 14.12 -7.51 4.55 3.41 14.02 8.54 2.80 0.11 7.20 14.80 15.44 -0.57
Golgi -5.17 -0.59 -14.45 -11.24 -6.07 10.84 6.59 -9.54 -18.38 -12.43 8.14 5.55 -13.21 -21.34 -13.21
Lipid Particles -3.55 -3.45 -7.48 -4.41 -5.48 -3.38 -4.03 -6.80 -3.43 1.21 -5.17 -4.68 -10.35 -5.55 -1.04
Mitochondria 0.67 -1.51 -6.07 -7.29 -0.43 -5.48 0.66 -7.41 -2.05 -4.85 -4.41 -1.11 -10.22 -6.45 -3.00
Mitotic Spindle -2.05 -2.57 -5.22 -3.38 1.83 -2.14 -3.03 -6.28 -3.53 2.46 -3.40 -4.00 -8.13 -4.74 3.02
None 1.23 0.08 0.77 -2.22 1.73 1.68 2.24 2.00 0.89 -3.21 1.93 2.01 1.99 0.11 -0.26
Nuclear Periphery -3.17 -4.21 -7.97 -4.94 3.91 -5.23 -3.26 -8.65 0.45 1.99 -6.52 -4.75 -12.24 -2.50 4.10
Nuclear Periphery Foci -5.19 -4.55 -6.63 -1.97 3.74 -4.32 -3.10 -6.05 1.66 2.75 -6.20 -4.85 -8.90 0.21 4.13
Nucleolus -0.21 -3.29 -3.89 -3.87 3.16 -0.63 -2.45 0.96 1.41 2.49 -0.24 -4.06 -1.90 -1.60 3.98
Nucleus 1.75 -2.89 1.64 -0.44 3.07 1.13 -1.75 2.23 3.51 2.49 1.58 -3.22 2.32 2.96 3.91
Peroxisomes -0.31 -3.74 -5.76 -5.62 2.07 -2.85 -6.43 -8.55 -4.41 5.69 -3.34 -7.23 -10.85 -7.11 5.93
Vacuole 3.96 -2.01 3.36 -0.76 3.97 -6.57 0.53 -5.10 1.20 -2.71 -4.80 -1.74 -4.29 0.43 -0.11
Vacuole Periphery 11.50 3.72 12.55 1.69 3.66 -7.15 9.51 1.47 8.48 -8.58 0.38 6.08 7.48 7.70 -2.42