Standard name
Description Nuclear envelope protein; required for SPB insertion, SPB duplication, Kar5p localization near the SPB and nuclear fusion; interacts with Mps2p to tether half-bridge to core SPB; N-terminal acetylation by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; member of the SUN protein family (Sad1-UNC-84 homology)

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0.05 0 0 0.05 0.08 0.05 0.07 0.07 0.05 0 0 0 0.05 0 0 0.06 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0.15 0.05 0.06 0.05 0.05 0.09 0.08 0.1 0.14 0 0 0 0.07 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0.11 0.07 0.09 0.07 0.07 0.05 0.09 0 0.14 0.11 0.17 0.08 0.08 0.07 0.05 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.06 0 0 0.09 0.08 0.07 0 0.05 0 0 0.08 0.12 0.13 0.14 0.15 0.09 0.12 0.15 0.11 0.16 0.17 0.18
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.18 0.26 0.28 0.13 0.22 0.14 0.25 0.17 0.26 0.27 0.09 0.06 0 0.06 0.07 0.07 0 0.09 0 0 0 0
Nucleus 0 0.05 0 0 0.05 0.06 0.06 0.07 0.07 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.1 0 0.11 0 0
Nucleolus 0.76 0.42 0.24 0.32 0.33 0.25 0.33 0.37 0.34 0.26 0.35 0.3 0.32 0.18 0.19 0.14 0.08 0.15 0.05 0 0 0
Peroxisomes 0 0 0.06 0.1 0.08 0 0.06 0 0 0 0.05 0.06 0.1 0 0 0.05 0 0 0 0 0 0
SpindlePole 0.41 0.53 0.52 0.62 0.51 0.56 0.44 0.55 0.36 0.52 0.53 0.54 0.42 0.8 0.78 0.79 0.47 0.23 0.59 0.5 0.6 0.47
Vac/Vac Membrane 0.06 0 0 0 0 0 0 0 0 0 0 0.07 0.05 0 0 0 0 0.13 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 4
Bud 0 3 4 4 11 28 11 21 12 12 2 1 2 9 7 9 2 0 1 1 3 9
Bud Neck 0 1 0 1 1 1 1 4 2 1 2 3 2 5 3 4 0 0 0 1 2 6
Bud Site 0 10 7 11 13 18 20 26 17 32 5 6 4 13 9 13
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Cytoplasm 0 6 15 12 22 22 15 16 16 8 21 30 36 16 21 28 2 0 0 0 1 2
Endoplasmic Reticulum 0 0 1 0 2 0 0 0 0 0 0 1 1 1 6 3 0 0 0 0 0 0
Endosome 1 0 4 15 18 23 10 16 3 6 12 33 29 27 40 33 5 2 8 15 32 48
Golgi 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 3 0 0 0 0 2 3
Mitochondria 3 17 37 22 53 48 56 53 44 61 14 17 8 11 20 25 1 1 2 4 6 9
Nucleus 0 3 4 4 13 19 14 22 12 18 5 7 9 0 4 2 0 0 1 0 1 3
Nuclear Periphery 0 0 2 2 2 7 4 4 2 5 2 6 6 0 0 0 1 1 3 10 6 8
Nucleolus 13 28 32 56 80 85 73 113 58 58 54 79 69 35 52 55 3 2 3 2 1 5
Peroxisomes 0 0 8 18 20 14 13 13 4 9 8 15 21 3 5 19 0 0 0 1 2 10
SpindlePole 7 35 68 108 121 190 98 168 61 118 81 143 92 154 212 301 21 3 47 47 116 130
Vac/Vac Membrane 1 1 5 2 4 8 4 6 3 8 6 18 10 3 10 12 0 2 2 0 4 9
Unique Cell Count 17 66 132 175 239 338 223 307 169 227 153 265 217 193 273 382 46 17 81 96 196 275
Labelled Cell Count 25 104 187 255 360 464 319 462 234 336 212 360 289 278 389 508 46 17 81 96 196 275


SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.0 4.5 5.3 6.0 5.2 5.6 5.0 5.3 5.2 5.0 6.7 6.9 6.5 5.9 6.0 6.1 5.8 6.5 6.4
Std Deviation (1e-4) 0.9 1.1 2.0 1.9 2.0 2.0 1.8 1.9 1.7 1.6 1.8 1.9 1.7 1.2 1.2 1.5 1.3 1.6 1.4
Intensity Change (Log2) 0.23 0.39 0.2 0.3 0.13 0.23 0.19 0.13 0.57 0.6 0.52 0.38 0.4 0.43 0.35 0.52 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 1.0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -2.3 -2.2 0 0 -1.4 -1.7 -1.1 -0.2 0 0 0 -2.1 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.5 0 0 0 0 0 0.1 0 1.0 0.5 1.5 -0.2 -0.4 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 3.0 3.1 3.2 3.3 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.3 -2.5 -0.6 -2.3 -0.1 -1.6 0 0.2 -3.2 -4.6 -5.5 -4.5 -4.3 -4.9
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -2.6 -1.5 -1.3 -2.9 -1.5 -0.9 -1.2 -2.6 -1.0 -2.0 -1.6 -4.0 -4.0 -5.4
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole -0.2 1.2 -0.3 0.5 -1.3 0.3 -2.4 -0.2 0 0.1 -1.5 4.2 4.0 4.5
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mps3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available