Standard name | |
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Human Ortholog | |
Description | Nuclear envelope protein; required for SPB insertion, SPB duplication, Kar5p localization near the SPB and nuclear fusion; interacts with Mps2p to tether half-bridge to core SPB; N-terminal acetylation by Eco1p regulates its role in nuclear organization; localizes to the SPB half bridge and telomeres during meiosis; required with Ndj1p and Csm4p for meiotic bouquet formation and telomere-led rapid prophase movement; member of the SUN protein family (Sad1-UNC-84 homology) |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | – | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 4 |
Bud | 0 | – | 3 | 4 | 4 | 11 | – | 28 | 11 | 21 | 12 | 12 | 2 | 1 | 2 | 9 | 7 | 9 | 2 | 0 | 1 | 1 | 3 | 9 |
Bud Neck | 0 | – | 1 | 0 | 1 | 1 | – | 1 | 1 | 4 | 2 | 1 | 2 | 3 | 2 | 5 | 3 | 4 | 0 | 0 | 0 | 1 | 2 | 6 |
Bud Site | 0 | – | 10 | 7 | 11 | 13 | – | 18 | 20 | 26 | 17 | 32 | 5 | 6 | 4 | 13 | 9 | 13 | – | – | – | – | – | – |
Cell Periphery | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0 | – | 6 | 15 | 12 | 22 | – | 22 | 15 | 16 | 16 | 8 | 21 | 30 | 36 | 16 | 21 | 28 | 2 | 0 | 0 | 0 | 1 | 2 |
Endoplasmic Reticulum | 0 | – | 0 | 1 | 0 | 2 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 6 | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1 | – | 0 | 4 | 15 | 18 | – | 23 | 10 | 16 | 3 | 6 | 12 | 33 | 29 | 27 | 40 | 33 | 5 | 2 | 8 | 15 | 32 | 48 |
Golgi | 0 | – | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 2 | 3 |
Mitochondria | 3 | – | 17 | 37 | 22 | 53 | – | 48 | 56 | 53 | 44 | 61 | 14 | 17 | 8 | 11 | 20 | 25 | 1 | 1 | 2 | 4 | 6 | 9 |
Nucleus | 0 | – | 3 | 4 | 4 | 13 | – | 19 | 14 | 22 | 12 | 18 | 5 | 7 | 9 | 0 | 4 | 2 | 0 | 0 | 1 | 0 | 1 | 3 |
Nuclear Periphery | 0 | – | 0 | 2 | 2 | 2 | – | 7 | 4 | 4 | 2 | 5 | 2 | 6 | 6 | 0 | 0 | 0 | 1 | 1 | 3 | 10 | 6 | 8 |
Nucleolus | 13 | – | 28 | 32 | 56 | 80 | – | 85 | 73 | 113 | 58 | 58 | 54 | 79 | 69 | 35 | 52 | 55 | 3 | 2 | 3 | 2 | 1 | 5 |
Peroxisomes | 0 | – | 0 | 8 | 18 | 20 | – | 14 | 13 | 13 | 4 | 9 | 8 | 15 | 21 | 3 | 5 | 19 | 0 | 0 | 0 | 1 | 2 | 10 |
SpindlePole | 7 | – | 35 | 68 | 108 | 121 | – | 190 | 98 | 168 | 61 | 118 | 81 | 143 | 92 | 154 | 212 | 301 | 21 | 3 | 47 | 47 | 116 | 130 |
Vac/Vac Membrane | 1 | – | 1 | 5 | 2 | 4 | – | 8 | 4 | 6 | 3 | 8 | 6 | 18 | 10 | 3 | 10 | 12 | 0 | 2 | 2 | 0 | 4 | 9 |
Unique Cell Count | 17 | 66 | 132 | 175 | 239 | 338 | 223 | 307 | 169 | 227 | 153 | 265 | 217 | 193 | 273 | 382 | 46 | 17 | 81 | 96 | 196 | 275 | ||
Labelled Cell Count | 25 | 104 | 187 | 255 | 360 | 464 | 319 | 462 | 234 | 336 | 212 | 360 | 289 | 278 | 389 | 508 | 46 | 17 | 81 | 96 | 196 | 275 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.0 | – | 4.5 | 5.3 | 6.0 | 5.2 | – | 5.6 | 5.0 | 5.3 | 5.2 | 5.0 | 6.7 | 6.9 | 6.5 | 5.9 | 6.0 | 6.1 | 5.8 | 6.5 | 6.4 |
Std Deviation (1e-4) | 0.9 | – | 1.1 | 2.0 | 1.9 | 2.0 | – | 2.0 | 1.8 | 1.9 | 1.7 | 1.6 | 1.8 | 1.9 | 1.7 | 1.2 | 1.2 | 1.5 | 1.3 | 1.6 | 1.4 |
Intensity Change (Log2) | – | – | – | 0.23 | 0.39 | 0.2 | – | 0.3 | 0.13 | 0.23 | 0.19 | 0.13 | 0.57 | 0.6 | 0.52 | 0.38 | 0.4 | 0.43 | 0.35 | 0.52 | 0.5 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | – | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | -2.3 | -2.2 | 0 | – | 0 | -1.4 | -1.7 | -1.1 | -0.2 | 0 | 0 | 0 | -2.1 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.5 | 0 | 0 | – | 0 | 0 | 0 | 0.1 | 0 | 1.0 | 0.5 | 1.5 | -0.2 | -0.4 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 3.0 | 3.1 | 3.2 | 3.3 | 0 |
Golgi | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0.3 | -2.5 | -0.6 | – | -2.3 | -0.1 | -1.6 | 0 | 0.2 | -3.2 | -4.6 | -5.5 | -4.5 | -4.3 | -4.9 |
Nucleus | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | -2.6 | -1.5 | -1.3 | – | -2.9 | -1.5 | -0.9 | -1.2 | -2.6 | -1.0 | -2.0 | -1.6 | -4.0 | -4.0 | -5.4 |
Peroxisomes | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | -0.2 | 1.2 | -0.3 | – | 0.5 | -1.3 | 0.3 | -2.4 | -0.2 | 0 | 0.1 | -1.5 | 4.2 | 4.0 | 4.5 |
Vacuole | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Actin | 0.5582 | 0.0033 | 0.0366 | 0.0007 | 0.0208 | 0.0174 | 0.136 | 0.004 | 0.0284 | 0.0388 | 0.0001 | 0.0026 | – | – | – | – | – | – |
Bud | 0.0022 | 0.0014 | 0.0006 | 0.0001 | 0.0011 | 0 | 0.0047 | 0.0005 | 0.0002 | 0.0006 | 0 | 0.0005 | – | – | – | – | – | – |
Bud Neck | 0.0022 | 0.0024 | 0.0011 | 0.0029 | 0.0052 | 0.0002 | 0.034 | 0.0035 | 0.0007 | 0.0165 | 0.0001 | 0.0086 | – | – | – | – | – | – |
Bud Periphery | 0.0029 | 0.001 | 0.0008 | 0.0001 | 0.0025 | 0.0002 | 0.0053 | 0.0006 | 0.0002 | 0.0015 | 0 | 0.0009 | – | – | – | – | – | – |
Bud Site | 0.0055 | 0.0132 | 0.0054 | 0.0003 | 0.0148 | 0 | 0.0864 | 0.0161 | 0.0011 | 0.0141 | 0 | 0.0145 | – | – | – | – | – | – |
Cell Periphery | 0.0005 | 0.0001 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0024 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0001 | – | – | – | – | – | – |
Cytoplasm | 0.01 | 0.0065 | 0.0011 | 0.0007 | 0.0021 | 0.0001 | 0.1116 | 0.0116 | 0.0014 | 0.0326 | 0.0001 | 0.0055 | – | – | – | – | – | – |
Cytoplasmic Foci | 0.0083 | 0.0097 | 0.0115 | 0.0594 | 0.038 | 0.0243 | 0.0558 | 0.0266 | 0.045 | 0.019 | 0.0025 | 0.026 | – | – | – | – | – | – |
Eisosomes | 0.0021 | 0.0001 | 0.0002 | 0 | 0.0005 | 0.0005 | 0.0023 | 0.0001 | 0.0001 | 0.0004 | 0 | 0.0001 | – | – | – | – | – | – |
Endoplasmic Reticulum | 0.0375 | 0.0018 | 0.0015 | 0.001 | 0.0033 | 0.0014 | 0.0453 | 0.0058 | 0.0018 | 0.009 | 0.0001 | 0.0016 | – | – | – | – | – | – |
Endosome | 0.1233 | 0.0118 | 0.0029 | 0.0113 | 0.0281 | 0.0053 | 0.1349 | 0.016 | 0.0115 | 0.0166 | 0.0003 | 0.0414 | – | – | – | – | – | – |
Golgi | 0.0278 | 0.0017 | 0.0032 | 0.0053 | 0.0167 | 0.016 | 0.0293 | 0.0049 | 0.0138 | 0.0061 | 0.0002 | 0.005 | – | – | – | – | – | – |
Lipid Particles | 0.0098 | 0.0099 | 0.0297 | 0.0991 | 0.0823 | 0.026 | 0.0514 | 0.0203 | 0.052 | 0.0145 | 0.0016 | 0.0896 | – | – | – | – | – | – |
Mitochondria | 0.0135 | 0.0015 | 0.0012 | 0.0004 | 0.0496 | 0.0061 | 0.0172 | 0.0026 | 0.0074 | 0.0318 | 0 | 0.0025 | – | – | – | – | – | – |
None | 0.0154 | 0.0015 | 0.0011 | 0.0008 | 0.0026 | 0.0002 | 0.1234 | 0.0047 | 0.0009 | 0.0136 | 0.0001 | 0.002 | – | – | – | – | – | – |
Nuclear Periphery | 0.1408 | 0.0577 | 0.0346 | 0.1427 | 0.0136 | 0.0064 | 0.0512 | 0.0894 | 0.0815 | 0.2068 | 0.1747 | 0.255 | – | – | – | – | – | – |
Nucleolus | 0.001 | 0.0922 | 0.0312 | 0.0298 | 0.1053 | 0.0044 | 0.0068 | 0.0467 | 0.0274 | 0.0176 | 0.0027 | 0.0141 | – | – | – | – | – | – |
Nucleus | 0.0131 | 0.0472 | 0.0178 | 0.0045 | 0.0157 | 0.0012 | 0.0376 | 0.0513 | 0.0175 | 0.0244 | 0.0028 | 0.0432 | – | – | – | – | – | – |
Peroxisomes | 0.0027 | 0.0015 | 0.0047 | 0.0138 | 0.0101 | 0.0015 | 0.0149 | 0.0023 | 0.009 | 0.0027 | 0.0001 | 0.021 | – | – | – | – | – | – |
Punctate Nuclear | 0.0116 | 0.7285 | 0.8139 | 0.6237 | 0.5707 | 0.8875 | 0.0348 | 0.6882 | 0.6983 | 0.524 | 0.8143 | 0.4562 | – | – | – | – | – | – |
Vacuole | 0.0037 | 0.0054 | 0.0002 | 0.0007 | 0.0072 | 0.0001 | 0.0098 | 0.0022 | 0.0006 | 0.0034 | 0 | 0.0047 | – | – | – | – | – | – |
Vacuole Periphery | 0.0079 | 0.0016 | 0.0004 | 0.0026 | 0.009 | 0.001 | 0.0049 | 0.0023 | 0.0013 | 0.0056 | 0.0003 | 0.0051 | – | – | – | – | – | – |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 21.5404 | 22.1441 | 8.5732 | 15.3849 | 20.5969 | 13.9935 | 11.6817 | 11.0052 | 13.4613 | 23.4192 |
Translational Efficiency | 1.4001 | 0.5684 | 0.9473 | 0.7456 | 1.0293 | 2.0806 | 0.8857 | 0.664 | 0.7161 | 0.8395 |
Dataset | Hit |
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Protein Concentration | – |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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