Standard name
Human Ortholog
Description Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 – 0 0.05 0.07 0.05 0.06 0 0 0 0 0 0 0.07 0.1 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 0 0 0 0.06 0.1 0.06 0.16 0.14 0.31 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 – 0.93 0.89 0.91 0.9 0.83 0.87 0.81 0.87 0.73 0.83 0.93 0.89 0.85 0.88 0.88 0.91 0.87 0.91 0.91 0.82 0.7 0.67
Nuclear Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 – 0.12 0.19 0.05 0.13 0.17 0.18 0.29 0.2 0.31 0.31 0.11 0.09 0.1 0.06 0.09 0.1 0.08 0 0 0.11 0.18 0.19
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 1 1 3 0 0 1 0 0 0 1 0 1 1 0 0 0 1 2
Bud Neck 0 – 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 0 0 0 1 5 5 3 8 7 9 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 – 0 8 19 13 25 7 8 5 7 4 4 16 24 6 3 3 0 0 0 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 1 0 0 0 0 0 0 0 2 1 0 0 1 0 0 0 1 2
Golgi 0 – 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0
Mitochondria 3 – 0 2 1 15 44 19 60 45 79 45 1 1 1 6 0 3 2 0 0 0 3 4
Nucleus 259 – 38 134 250 226 357 294 307 276 189 225 166 208 195 207 107 177 252 41 58 134 198 238
Nuclear Periphery 0 – 0 0 3 2 4 1 0 3 0 0 0 0 0 0 3 0 0 0 0 0 0 1
Nucleolus 48 – 5 29 13 32 73 62 110 64 81 84 19 21 23 15 11 19 23 1 1 17 50 67
Peroxisomes 0 – 0 0 0 0 0 0 0 0 1 1 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 1 2 2 0 1 1 1 2 4 4 2 0 0 0 0 0 1 1 3
Vac/Vac Membrane 1 – 0 1 1 0 1 1 2 1 1 2 1 2 0 7 5 3 5 2 1 4 20 32
Unique Cell Count 278 41 150 276 252 432 339 379 317 258 270 178 235 229 235 121 194 291 46 64 164 285 357
Labelled Cell Count 317 43 174 288 290 513 394 493 403 366 372 193 252 252 250 132 206 291 46 64 164 285 357


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 – 8.1 7.7 8.5 6.9 6.3 6.7 6.0 6.2 5.6 5.9 10.0 9.9 9.3 12.5 13.7 13.2 8.6 9.0 9.7
Std Deviation (1e-4) 2.8 – 1.6 1.6 2.3 1.9 1.7 1.5 1.7 1.7 1.8 1.5 2.4 3.1 2.5 4.0 3.9 3.1 2.1 1.9 1.9
Intensity Change (Log2) – – – -0.07 0.07 -0.23 -0.36 -0.27 -0.43 -0.39 -0.55 -0.47 0.3 0.28 0.19 0.62 0.75 0.7 0.08 0.14 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.8 2.6 4.1 2.8 0 0 0 0 0 0
Nucleus -0.6 -0.4 -0.6 -1.7 -1.1 -1.9 -1.0 -2.7 -1.5 0.1 -0.8 -1.3 -0.9 -0.8 -0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.1 0 0.1 0.8 1.0 2.3 1.2 2.5 2.5 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.1886 5.9966 5.7259 5.475 4.8931 5.465 4.6731 5.9663 5.1413 4.4302 4.183 4.8718 4.2034 5.0782 4.5405 4.405 3.0204 4.0859
Actin 0.0067 0.0011 0.0009 0.0356 0.0007 0.0012 0.0014 0 0.0001 0 0 0.0012 0.0187 0 0.0069 0 0.0015 0.0005
Bud 0.0006 0.001 0.0005 0.0028 0.0002 0.0001 0.0001 0 0 0 0 0 0.0004 0.0003 0.0006 0 0.0001 0.0002
Bud Neck 0.0011 0.0002 0.0001 0.0004 0.0003 0.0006 0.0074 0 0.0002 0 0.0006 0.0005 0.0002 0 0.0002 0 0.0002 0.0004
Bud Periphery 0.001 0.0007 0.0004 0.0033 0.0003 0.0001 0.0003 0 0 0 0.0001 0.0001 0.0009 0.0002 0.0002 0 0.0002 0.0009
Bud Site 0.002 0.003 0.0011 0.0143 0.0003 0.0001 0.0018 0 0.0002 0 0.0001 0.0001 0.0012 0.0008 0.0005 0 0.0002 0.0001
Cell Periphery 0.0003 0.0002 0.0001 0.0001 0.0001 0 0.0001 0 0 0 0 0 0.0002 0.0001 0.0001 0 0 0.0001
Cytoplasm 0.004 0.0048 0.0021 0.0021 0.0014 0.0002 0.0007 0 0.0001 0 0.0001 0.0002 0.0012 0.0151 0.0134 0 0.0001 0.0006
Cytoplasmic Foci 0.0121 0.0021 0.0102 0.0342 0.0055 0.0004 0.0034 0 0 0 0 0 0.0175 0.0001 0.002 0 0.0042 0.0002
Eisosomes 0.0001 0 0 0.0001 0 0 0.0001 0 0 0 0 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0015 0.002 0.0008 0.0003 0.0016 0.0002 0.0003 0 0.0001 0 0 0.0004 0.0019 0 0.0001 0 0.0001 0.0006
Endosome 0.0142 0.0075 0.0104 0.0107 0.0309 0.0012 0.0039 0 0 0 0 0.0008 0.0117 0.0001 0.0002 0 0.0215 0.0066
Golgi 0.0043 0.0012 0.0026 0.0056 0.0037 0.0003 0.0007 0 0 0 0 0.0001 0.0062 0 0.0007 0 0.0421 0.0003
Lipid Particles 0.0105 0.0004 0.0017 0.0048 0.0352 0.0014 0.0069 0 0 0 0 0.0001 0.0044 0 0.001 0 0.0031 0.0002
Mitochondria 0.008 0.0051 0.0041 0.0012 0.0091 0.0011 0.002 0 0.0003 0 0.0004 0.0002 0.0056 0.0004 0.0006 0.0001 0.0898 0.0032
None 0.0068 0.0008 0.0009 0.0003 0.0006 0.0002 0.0029 0 0 0 0 0.0003 0.004 0 0 0 0.0001 0.0003
Nuclear Periphery 0.0154 0.0032 0.0027 0.0005 0.0044 0.0127 0.0024 0.0002 0.0003 0.0001 0.0017 0.0019 0.0067 0.0007 0.0004 0.0001 0.0046 0.0207
Nucleolus 0.0216 0.01 0.0102 0.0067 0.1373 0.0707 0.0178 0.0099 0.0102 0.0416 0.2127 0.0648 0.0168 0.0199 0.0065 0.0039 0.0742 0.043
Nucleus 0.8571 0.9515 0.9368 0.8681 0.749 0.9037 0.9363 0.9897 0.9881 0.958 0.7832 0.9278 0.8916 0.9618 0.9585 0.996 0.747 0.9144
Peroxisomes 0.01 0.0004 0.0052 0.0074 0.0161 0.0006 0.0035 0 0 0 0 0 0.0023 0 0.0079 0 0.0022 0.0001
Punctate Nuclear 0.0155 0.0028 0.0069 0.0014 0.0015 0.0047 0.0067 0.0001 0.0002 0.0003 0.0009 0.0012 0.0027 0.0001 0.0001 0 0.0069 0.0007
Vacuole 0.0037 0.0014 0.0017 0.0002 0.001 0.0002 0.0007 0 0.0001 0 0.0001 0.0002 0.003 0.0002 0.0001 0 0.0006 0.0024
Vacuole Periphery 0.0034 0.0006 0.0008 0.0001 0.0009 0.0004 0.0005 0 0 0 0.0001 0.0001 0.0026 0.0001 0 0 0.0012 0.0045

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 44.6464 40.8614 56.4111 54.1019 56.5078 52.0587 56.2076 66.4696 61.9461 55.8933
Translational Efficiency 2.4089 2.1917 1.3593 1.246 1.3341 2.0702 1.2466 1.1387 1.0507 1.2335

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1220 763 2291 1424 1352 1300 533 1217 2572 2063 2824 2641

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 908.64 916.89 1212.67 1309.73 898.87 1037.39 1236.65 1326.78 903.50 992.82 1217.20 1317.59
Standard Deviation 157.17 250.57 198.47 231.90 175.64 173.97 203.89 228.16 167.20 213.72 199.73 230.34
Intensity Change Log 2 — 0.013040 0.416406 0.527489 — 0.206774 0.460253 0.561745 — 0.112634 0.438378 0.544626

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000551 0.001060 0.000719 0.000946 0.000605 0.000775 0.001311 0.000931 0.000579 0.000880 0.000830 0.000939
Bud Neck 0.003437 0.005314 0.016209 0.019361 0.006088 0.013378 0.024668 0.024979 0.004830 0.010396 0.017806 0.021950
Bud Site 0.000297 0.001403 0.001480 0.003022 0.000557 0.002181 0.003514 0.002912 0.000434 0.001894 0.001864 0.002971
Cell Periphery 0.000049 0.000297 0.000061 0.000093 0.000078 0.000342 0.000095 0.000106 0.000064 0.000326 0.000067 0.000099
Cytoplasm 0.000106 0.003604 0.000191 0.000432 0.000317 0.000440 0.000927 0.001046 0.000217 0.001610 0.000330 0.000715
Cytoplasmic Foci 0.000047 0.001015 0.000060 0.001108 0.000094 0.002426 0.000192 0.001369 0.000072 0.001904 0.000085 0.001229
Eisosomes 0.000042 0.000053 0.000076 0.000042 0.000049 0.000062 0.000079 0.000047 0.000046 0.000059 0.000077 0.000044
Endoplasmic Reticulum 0.002148 0.002590 0.001078 0.001422 0.002186 0.002858 0.001601 0.001253 0.002168 0.002759 0.001177 0.001345
Endosome 0.000083 0.000509 0.000343 0.001796 0.000216 0.001569 0.000263 0.001877 0.000153 0.001177 0.000328 0.001833
Golgi 0.000092 0.000618 0.000104 0.001035 0.000109 0.001170 0.000249 0.003346 0.000101 0.000966 0.000132 0.002100
Lipid Particles 0.000188 0.000440 0.000017 0.000317 0.000102 0.000795 0.000047 0.000237 0.000142 0.000663 0.000023 0.000280
Mitochondria 0.001192 0.001298 0.001352 0.003311 0.000908 0.002225 0.003535 0.003930 0.001043 0.001883 0.001764 0.003596
Mitotic Spindle 0.001447 0.002427 0.001115 0.009096 0.001494 0.005934 0.003911 0.009051 0.001472 0.004637 0.001643 0.009075
None 0.002039 0.011504 0.000660 0.000868 0.002603 0.001224 0.001988 0.002280 0.002335 0.005026 0.000911 0.001518
Nuclear Periphery 0.000099 0.000097 0.000269 0.001401 0.000145 0.000321 0.000495 0.000790 0.000123 0.000238 0.000312 0.001120
Nuclear Periphery Foci 0.000758 0.000804 0.000241 0.001518 0.000637 0.000782 0.000321 0.000389 0.000694 0.000790 0.000256 0.000998
Nucleolus 0.062036 0.054041 0.022893 0.018843 0.058064 0.052943 0.043406 0.019864 0.059948 0.053349 0.026765 0.019314
Nucleus 0.924078* 0.910189* 0.951392* 0.932114* 0.924184* 0.905029* 0.908891* 0.920282* 0.924134* 0.906937* 0.943371* 0.926662*
Peroxisomes 0.000864 0.001536 0.000476 0.000448 0.000863 0.001275 0.001548 0.000652 0.000863 0.001372 0.000678 0.000542
Vacuole 0.000312 0.000905 0.000695 0.001605 0.000399 0.002530 0.002253 0.002778 0.000358 0.001929 0.000989 0.002146
Vacuole Periphery 0.000137 0.000294 0.000568 0.001221 0.000304 0.001739 0.000705 0.001879 0.000225 0.001205 0.000594 0.001524

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -3.26 -1.85 -3.70 0.53 -1.54 -1.85 -7.07 -3.94 -2.47 4.43 -3.84 -3.52 -5.16 -1.01 -0.69
Bud Neck -3.63 -16.65 -11.78 -10.05 -2.35 -6.43 -9.75 -11.48 -6.52 0.85 -7.57 -17.78 -16.23 -9.93 -3.10
Bud Site -4.21 -4.38 -4.65 -2.72 -2.51 -3.42 -4.68 -4.98 -1.09 1.15 -4.64 -5.73 -6.55 -2.35 -2.37
Cell Periphery -3.66 -0.85 -2.71 2.81 -2.06 -1.11 -1.25 -0.98 1.00 0.02 -1.72 -0.42 -2.39 1.47 -2.06
Cytoplasm -2.28 -1.14 -3.57 1.99 -1.40 -1.41 -2.35 -1.69 -0.80 -0.22 -2.64 -1.48 -2.54 1.49 -1.50
Cytoplasmic Foci -1.78 -0.47 -2.37 0.28 -2.34 -2.69 -0.91 -2.50 1.34 -2.28 -3.18 -0.14 -3.42 1.41 -3.42
Eisosomes -3.24 -7.69 -0.61 2.52 6.86 -3.91 -4.28 0.75 4.50 4.56 -5.47 -8.14 0.28 5.39 8.09
Endoplasmic Reticulum -1.81 7.77 3.24 4.01 -2.39 -2.82 0.34 5.95 6.97 3.28 -3.41 8.17 5.89 7.48 -1.21
Endosome -2.37 -1.45 -3.31 -2.02 -2.57 -3.89 -0.45 -2.57 0.20 -2.47 -4.54 -1.10 -4.13 -0.72 -3.40
Golgi -1.83 -0.31 -2.33 -0.54 -2.28 -2.17 -2.34 -2.34 -1.23 -2.16 -2.68 -0.92 -3.00 -1.15 -2.93
Lipid Particles -0.93 1.16 -0.64 0.52 -2.21 -2.44 0.14 -0.93 2.05 -1.16 -2.51 1.45 -1.08 1.97 -2.74
Mitochondria -0.27 -0.63 -4.04 -5.39 -5.29 -3.18 -7.52 -5.27 -2.60 0.77 -2.54 -4.04 -6.81 -4.40 -4.69
Mitotic Spindle -0.62 0.16 -4.03 -3.11 -4.25 -2.44 -3.11 -3.15 -0.98 -0.26 -2.48 -1.23 -5.10 -2.59 -4.49
None -3.96 2.92 2.21 4.47 -2.70 2.33 0.95 0.50 -1.10 -0.11 -2.83 3.88 1.62 3.66 -1.60
Nuclear Periphery 0.01 -2.76 -3.61 -3.62 -3.03 -2.34 -5.07 -4.21 -2.91 -0.03 -2.45 -4.21 -4.86 -4.22 -3.47
Nuclear Periphery Foci -0.11 0.81 -0.70 -0.86 -1.97 -0.62 0.42 0.84 1.64 0.76 -0.51 1.15 -0.37 0.12 -1.87
Nucleolus 1.81 13.34 13.55 8.75 1.46 1.42 2.00 12.03 10.04 6.01 2.38 14.36 18.11 13.55 3.97
Nucleus 2.38 -6.25 -0.27 -2.29 4.28 3.69 4.56 1.31 -1.86 -3.11 4.42 -4.05 0.68 -3.18 3.72
Peroxisomes -3.11 3.72 5.04 4.81 0.75 -1.42 -4.39 1.13 1.88 5.44 -2.43 0.79 3.45 3.60 2.73
Vacuole -2.17 -1.48 -3.59 -1.58 -2.31 -2.78 -2.65 -2.76 0.05 0.38 -3.22 -2.81 -4.12 -0.05 -1.90
Vacuole Periphery -3.56 -1.50 -2.47 -2.03 -1.11 -2.32 -1.74 -2.41 0.18 -1.47 -2.52 -1.55 -3.39 -0.22 -1.84
Standard name
Human Ortholog
Description Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II
Localization
Cell Percentages nucleus (100%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Sua7

Sua7


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sua7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available