Standard name
Description Transcription factor TFIIB; a general transcription factor required for transcription initiation and start site selection by RNA polymerase II

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0.05 0.07 0.05 0.06 0 0 0 0 0 0 0.07 0.1 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.06 0.1 0.06 0.16 0.14 0.31 0.17 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.93 0.93 0.89 0.91 0.9 0.83 0.87 0.81 0.87 0.73 0.83 0.93 0.89 0.85 0.88 0.88 0.91 0.87 0.91 0.91 0.82 0.7 0.67
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.17 0.12 0.19 0.05 0.13 0.17 0.18 0.29 0.2 0.31 0.31 0.11 0.09 0.1 0.06 0.09 0.1 0.08 0 0 0.11 0.18 0.19
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0.07 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 1 3 0 0 1 0 0 0 1 0 1 1 0 0 0 1 2
Bud Neck 0 0 0 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 5 5 3 8 7 9 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 6 0 8 19 13 25 7 8 5 7 4 4 16 24 6 3 3 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 5 3 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 1 0 0 0 0 0 0 0 2 1 0 0 1 0 0 0 1 2
Golgi 0 0 0 1 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0
Mitochondria 3 0 2 1 15 44 19 60 45 79 45 1 1 1 6 0 3 2 0 0 0 3 4
Nucleus 259 38 134 250 226 357 294 307 276 189 225 166 208 195 207 107 177 252 41 58 134 198 238
Nuclear Periphery 0 0 0 3 2 4 1 0 3 0 0 0 0 0 0 3 0 0 0 0 0 0 1
Nucleolus 48 5 29 13 32 73 62 110 64 81 84 19 21 23 15 11 19 23 1 1 17 50 67
Peroxisomes 0 0 0 0 0 0 0 0 0 1 1 0 0 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 2 2 0 1 1 1 2 4 4 2 0 0 0 0 0 1 1 3
Vac/Vac Membrane 1 0 1 1 0 1 1 2 1 1 2 1 2 0 7 5 3 5 2 1 4 20 32
Unique Cell Count 278 41 150 276 252 432 339 379 317 258 270 178 235 229 235 121 194 291 46 64 164 285 357
Labelled Cell Count 317 43 174 288 290 513 394 493 403 366 372 193 252 252 250 132 206 291 46 64 164 285 357


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.5 8.1 7.7 8.5 6.9 6.3 6.7 6.0 6.2 5.6 5.9 10.0 9.9 9.3 12.5 13.7 13.2 8.6 9.0 9.7
Std Deviation (1e-4) 2.8 1.6 1.6 2.3 1.9 1.7 1.5 1.7 1.7 1.8 1.5 2.4 3.1 2.5 4.0 3.9 3.1 2.1 1.9 1.9
Intensity Change (Log2) -0.07 0.07 -0.23 -0.36 -0.27 -0.43 -0.39 -0.55 -0.47 0.3 0.28 0.19 0.62 0.75 0.7 0.08 0.14 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.8 2.6 4.1 2.8 0 0 0 0 0 0
Nucleus -0.6 -0.4 -0.6 -1.7 -1.1 -1.9 -1.0 -2.7 -1.5 0.1 -0.8 -1.3 -0.9 -0.8 -0.3
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 1.1 0 0.1 0.8 1.0 2.3 1.2 2.5 2.5 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sua7-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available