| ORF | |
|---|---|
| Description | Protein that contains a PX domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; PX stands for Phox homology |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 3 | 5 |
| Bud | 0 | 1 | 1 | 2 | 0 | 0 | 1 | 5 | 2 | – | 11 | 5 | 0 | 0 | 5 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 7 | 5 |
| Bud Neck | 2 | 0 | 3 | 1 | 1 | 3 | 9 | 12 | 2 | – | 7 | 10 | 0 | 0 | 0 | 3 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 2 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 2 | – | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 2 | 2 | 0 | 2 | 0 | 0 | 1 | 0 | 2 | – | 0 | 1 | 0 | 0 | 1 | 0 | 2 | 2 | 0 | 1 | 2 | 1 | 3 | 2 |
| Cytoplasm | 258 | 248 | 179 | 205 | 305 | 306 | 393 | 447 | 274 | – | 204 | 262 | 314 | 413 | 440 | 211 | 146 | 173 | 219 | 212 | 98 | 182 | 258 | 284 |
| Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 1 | 0 | 0 | 0 | 14 | 4 | 17 | 1 | 0 | 1 | 0 | 1 | 4 |
| Endosome | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 4 | 1 | 2 | 0 | 3 | 3 |
| Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 | 1 | 0 | 0 | 0 | 0 |
| Mitochondria | 2 | 15 | 2 | 12 | 7 | 34 | 69 | 80 | 85 | – | 136 | 176 | 1 | 0 | 11 | 14 | 3 | 2 | 38 | 22 | 38 | 10 | 9 | 15 |
| Nucleus | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 2 | 1 | – | 2 | 0 | 1 | 1 | 2 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
| SpindlePole | 0 | 0 | 0 | 0 | 2 | 1 | 4 | 8 | 6 | – | 7 | 11 | 0 | 0 | 0 | 2 | 1 | 2 | 4 | 2 | 2 | 0 | 1 | 2 |
| Vac/Vac Membrane | 2 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | 2 | 5 | 2 | 2 | 4 | 5 | 1 | 5 | 5 |
| Unique Cell Count | 264 | 257 | 179 | 207 | 309 | 318 | 430 | 487 | 311 | 292 | 361 | 314 | 413 | 442 | 223 | 154 | 184 | 284 | 255 | 161 | 203 | 308 | 346 | |
| Labelled Cell Count | 269 | 270 | 185 | 222 | 317 | 344 | 483 | 555 | 375 | 374 | 468 | 316 | 414 | 460 | 251 | 165 | 206 | 284 | 255 | 161 | 203 | 308 | 346 | |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.1 | 3.1 | 2.9 | 2.9 | 3.2 | 2.9 | 2.5 | 3.0 | 2.4 | – | 2.3 | 2.0 | 3.9 | 4.0 | 3.5 | 5.2 | 5.6 | 5.5 | 3.8 | 3.9 | 4.1 |
| Std Deviation (1e-4) | 0.8 | 0.4 | 0.7 | 1.5 | 1.7 | 1.4 | 0.8 | 1.5 | 1.2 | – | 1.1 | 0.8 | 1.2 | 1.0 | 1.1 | 1.8 | 2.3 | 2.4 | 0.8 | 1.6 | 0.9 |
| Intensity Change (Log2) | – | – | – | 0.03 | 0.19 | 0.02 | -0.17 | 0.05 | -0.28 | – | -0.29 | -0.51 | 0.46 | 0.5 | 0.3 | 0.86 | 0.97 | 0.95 | 0.43 | 0.46 | 0.52 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
