ORF
Human Ortholog
Description Protein that contains a PX domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; PX stands for Phox homology
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 1.0 0.99 0.99 0.96 0.91 0.92 0.88 – 0.7 0.73 1.0 1.0 1.0 0.95 0.95 0.94 0.77 0.83 0.61 0.9 0.84 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.06 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.06 0 0.11 0.16 0.16 0.27 – 0.47 0.49 0 0 0 0.06 0 0 0.14 0.09 0.24 0.05 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 2 1 2 0 3 5
Bud 0 1 1 2 0 0 1 5 2 – 11 5 0 0 5 1 1 0 0 1 0 0 7 5
Bud Neck 2 0 3 1 1 3 9 12 2 – 7 10 0 0 0 3 1 1 1 0 0 1 0 2
Bud Site 0 0 0 0 0 0 2 0 2 – 2 1 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 0 2 0 0 1 0 2 – 0 1 0 0 1 0 2 2 0 1 2 1 3 2
Cytoplasm 258 248 179 205 305 306 393 447 274 – 204 262 314 413 440 211 146 173 219 212 98 182 258 284
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 1 – 1 1 0 0 0 14 4 17 1 0 1 0 1 4
Endosome 2 0 0 0 0 0 0 0 0 – 1 0 0 0 0 2 1 4 4 1 2 0 3 3
Golgi 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 1 2 1 0 0 0 0
Mitochondria 2 15 2 12 7 34 69 80 85 – 136 176 1 0 11 14 3 2 38 22 38 10 9 15
Nucleus 0 0 0 0 2 0 2 2 1 – 2 0 1 1 2 1 0 2 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 – 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 2 1 4 8 6 – 7 11 0 0 0 2 1 2 4 2 2 0 1 2
Vac/Vac Membrane 2 0 0 0 0 0 2 0 0 – 1 1 0 0 0 2 5 2 2 4 5 1 5 5
Unique Cell Count 264 257 179 207 309 318 430 487 311 292 361 314 413 442 223 154 184 284 255 161 203 308 346
Labelled Cell Count 269 270 185 222 317 344 483 555 375 374 468 316 414 460 251 165 206 284 255 161 203 308 346


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.1 2.9 2.9 3.2 2.9 2.5 3.0 2.4 – 2.3 2.0 3.9 4.0 3.5 5.2 5.6 5.5 3.8 3.9 4.1
Std Deviation (1e-4) 0.8 0.4 0.7 1.5 1.7 1.4 0.8 1.5 1.2 – 1.1 0.8 1.2 1.0 1.1 1.8 2.3 2.4 0.8 1.6 0.9
Intensity Change (Log2) – – – 0.03 0.19 0.02 -0.17 0.05 -0.28 – -0.29 -0.51 0.46 0.5 0.3 0.86 0.97 0.95 0.43 0.46 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.0843 0.0898 0.0802 -0.4811 -0.109 -0.0198 0.6505 0.7022 0.619 0.4881 0.9066 0.4841 0.0703 0.0919 0.4436 0.607 0.2717 0.8606
Actin 0.0289 0.0001 0.0412 0 0.0242 0.0007 0.0324 0.0003 0.0021 0.0082 0.0095 0.0022 0.002 0 0.0003 0.0001 0 0.0003
Bud 0.0015 0.0088 0.0003 0 0.0003 0.0003 0.002 0.0038 0.0009 0.0031 0.0012 0.0007 0.0001 0 0.0005 0 0 0
Bud Neck 0.0071 0.0001 0.005 0 0.0003 0.0006 0.0028 0.0001 0.0001 0.0025 0.0001 0.0011 0.0001 0 0 0 0 0.0003
Bud Periphery 0.0038 0.0012 0.0004 0 0.0006 0.0005 0.0032 0.0024 0.0008 0.004 0.0014 0.0009 0.0001 0 0.001 0 0 0.0001
Bud Site 0.0169 0.0007 0.0031 0 0.0004 0.0003 0.0161 0.0127 0.0049 0.0282 0.0004 0.0005 0.0003 0 0.0006 0 0 0
Cell Periphery 0.0005 0.0002 0.0003 0 0.0001 0.0001 0.0011 0.0004 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0.0003 0 0 0
Cytoplasm 0.0455 0.0421 0.035 0.0109 0.0735 0.0855 0.0442 0.0653 0.078 0.0512 0.0781 0.1257 0.0266 0.0207 0.016 0.0081 0.0102 0.0131
Cytoplasmic Foci 0.0239 0.0033 0.0074 0.0008 0.0046 0.0041 0.0191 0.0106 0.013 0.0105 0.0075 0.0261 0.0038 0.0006 0.0019 0.0007 0.0005 0.0021
Eisosomes 0.0005 0.0002 0.0005 0 0.0002 0.0001 0.0008 0.0001 0.0001 0.0003 0.0002 0.0001 0.0002 0.0001 0.0003 0.0004 0.0001 0.0003
Endoplasmic Reticulum 0.0046 0.0019 0.0012 0.0001 0.0054 0.0013 0.0085 0.0004 0.0009 0.0013 0.0013 0.0013 0.0006 0.0002 0.0012 0.0002 0.0001 0.0001
Endosome 0.0152 0.0054 0.0048 0.0001 0.016 0.0011 0.0188 0.0003 0.003 0.008 0.0383 0.0143 0.0009 0 0.0006 0 0 0.0006
Golgi 0.006 0.0003 0.0032 0 0.004 0.0001 0.006 0.0001 0.0022 0.0043 0.0215 0.0064 0.0001 0 0.0001 0 0 0.0011
Lipid Particles 0.0125 0.0008 0.0068 0 0.0029 0.0002 0.0106 0.0004 0.0014 0.0064 0.0132 0.0041 0.0002 0 0.0007 0.0001 0 0.0002
Mitochondria 0.004 0.0014 0.0015 0 0.0019 0.0003 0.0192 0.0004 0.0034 0.0215 0.0088 0.0046 0.0002 0 0.0005 0.0001 0 0.0141
None 0.763 0.9296 0.8681 0.9877 0.8249 0.8988 0.7387 0.8951 0.8815 0.8308 0.7702 0.7961 0.9542 0.9777 0.9744 0.9899 0.9888 0.9665
Nuclear Periphery 0.0173 0.0004 0.0011 0.0001 0.0308 0.0014 0.0335 0.0003 0.0006 0.0017 0.0134 0.0014 0.0013 0 0.0002 0.0001 0 0.0001
Nucleolus 0.0013 0.0003 0.0009 0 0.0003 0.0003 0.003 0.0004 0.0002 0.0015 0.0008 0.0018 0.0003 0 0.0003 0 0 0.0001
Nucleus 0.0237 0.0013 0.0014 0 0.003 0.0024 0.0137 0.0008 0.0007 0.0019 0.0174 0.0041 0.0022 0.0001 0.0002 0.0001 0 0.0001
Peroxisomes 0.0081 0.0001 0.0128 0 0.0006 0.0001 0.0033 0.0002 0.0025 0.0072 0.0069 0.0034 0.0003 0 0.0001 0 0 0.0004
Punctate Nuclear 0.0125 0.0005 0.0041 0.0001 0.0043 0.0006 0.0155 0.0052 0.0021 0.0039 0.0018 0.0032 0.0061 0 0.0002 0.0001 0 0.0003
Vacuole 0.0023 0.0012 0.0008 0.0001 0.0008 0.0012 0.0047 0.0007 0.0013 0.0025 0.0043 0.0012 0.0003 0.0001 0.0004 0 0 0.0001
Vacuole Periphery 0.0009 0.0002 0.0002 0 0.001 0.0002 0.0028 0.0001 0.0002 0.0006 0.0035 0.0006 0.0001 0 0.0001 0 0 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 30.9926 19.2867 32.2578 28.0749 24.4208 32.8579 22.2092 27.4724 23.8825 33.5552
Translational Efficiency 0.9482 0.9151 0.6117 0.6743 0.8256 0.7748 0.8776 0.5366 0.79 0.6821

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1794 1615 1036 1124 2302 999 1637 48 4096 2614 2673 1172

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 603.35 654.86 777.15 776.03 643.89 697.71 813.74 750.31 626.13 671.24 799.56 774.98
Standard Deviation 71.19 102.14 84.28 99.29 71.10 121.85 87.32 108.53 73.93 112.04 87.98 99.81
Intensity Change Log 2 — 0.118191 0.365198 0.363117 — 0.115813 0.337754 0.220673 — 0.116964 0.351095 0.291337

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000048 0.000233 0.000153 0.000439 0.000068 0.000864 0.000258 0.000172 0.000059 0.000474 0.000217 0.000428
Bud Neck 0.028662 0.035049 0.003610 0.009616 0.014898 0.050156 0.002547 0.002294 0.020926 0.040823 0.002959 0.009316
Bud Site 0.003289 0.011322 0.007264 0.034424 0.002795 0.019691 0.005718 0.008188 0.003011 0.014520 0.006317 0.033350
Cell Periphery 0.000256 0.000141 0.000171 0.000172 0.000064 0.000320 0.000142 0.000132 0.000148 0.000210 0.000154 0.000171
Cytoplasm 0.464624* 0.422410* 0.334854* 0.481329* 0.527949* 0.335952* 0.408435* 0.430013* 0.500214* 0.389368* 0.379916* 0.479227*
Cytoplasmic Foci 0.201519* 0.230256* 0.016641 0.023304 0.180497* 0.289419* 0.018330 0.082192 0.189704* 0.252866* 0.017675 0.025716
Eisosomes 0.000312 0.000133 0.000048 0.000050 0.000100 0.000228 0.000046 0.000072 0.000193 0.000169 0.000047 0.000050
Endoplasmic Reticulum 0.001007 0.000771 0.001576 0.001960 0.000524 0.000650 0.001150 0.001148 0.000736 0.000725 0.001315 0.001927
Endosome 0.011633 0.024380 0.002453 0.007747 0.006782 0.034571 0.001952 0.010212 0.008907 0.028275 0.002146 0.007848
Golgi 0.001187 0.003805 0.000083 0.002805 0.001217 0.012728 0.000056 0.017676 0.001204 0.007215 0.000067 0.003414
Lipid Particles 0.015028 0.010322 0.001767 0.001306 0.006981 0.010715 0.001379 0.001130 0.010505 0.010472 0.001529 0.001298
Mitochondria 0.004801 0.011803 0.000842 0.006467 0.003618 0.024728 0.000518 0.001126 0.004136 0.016743 0.000643 0.006249
Mitotic Spindle 0.000602 0.002930 0.003466 0.049385 0.001803 0.005238 0.002843 0.034289 0.001277 0.003812 0.003084 0.048767
None 0.004822 0.007805 0.006147 0.005706 0.002750 0.002168 0.005606 0.001992 0.003658 0.005651 0.005816 0.005554
Nuclear Periphery 0.000640 0.000636 0.001369 0.001663 0.000538 0.000388 0.001289 0.000724 0.000582 0.000542 0.001320 0.001624
Nuclear Periphery Foci 0.000276 0.000443 0.000489 0.001037 0.000223 0.000390 0.000515 0.000574 0.000246 0.000422 0.000505 0.001018
Nucleolus 0.001761 0.004096 0.001302 0.000470 0.001234 0.001830 0.000513 0.000306 0.001465 0.003230 0.000819 0.000463
Nucleus 0.194790* 0.141143* 0.583166* 0.258291* 0.203913* 0.104158 0.500104* 0.287961* 0.199917* 0.127009 0.532297* 0.259506*
Peroxisomes 0.004264 0.012322 0.000821 0.000937 0.003791 0.025125 0.000357 0.008401 0.003998 0.017215 0.000537 0.001242
Vacuole 0.059515 0.077082 0.033498 0.108417 0.039700 0.078179 0.048061 0.108997 0.048379 0.077501 0.042417 0.108440
Vacuole Periphery 0.000964 0.002916 0.000279 0.004474 0.000557 0.002500 0.000182 0.002403 0.000735 0.002757 0.000219 0.004389

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -7.92 -5.72 -7.25 -2.92 -3.65 -3.78 -1.87 -3.89 3.07 0.38 -5.07 -2.81 -7.14 1.01 -2.09
Bud Neck -2.19 12.83 11.40 12.46 -3.00 -9.76 11.12 10.19 13.47 0.31 -9.30 16.93 12.33 17.16 -4.48
Bud Site -7.87 -2.46 -5.87 -2.46 -3.37 -9.00 -2.15 -1.53 3.78 -0.70 -12.09 -3.24 -6.09 -1.08 -4.50
Cell Periphery 3.99 2.72 4.60 1.22 3.35 -4.39 -11.06 -2.91 3.18 0.47 -2.42 -0.23 1.59 3.49 2.62
Cytoplasm 4.95 15.44 10.54 5.65 -3.97 19.00 18.95 3.32 -1.32 -0.68 16.45 23.97 15.11 2.93 -1.70
Cytoplasmic Foci -4.17 41.86 42.50 39.27 0.74 -12.80 38.61 6.96 12.43 -2.70 -11.75 56.24 53.69 51.98 -0.22
Eisosomes 7.76 11.56 11.20 9.81 -2.53 -6.14 9.12 1.97 6.64 -2.83 1.76 13.45 12.42 11.49 -3.64
Endoplasmic Reticulum 1.19 -5.68 -8.70 -11.15 -3.59 -0.82 -11.27 -3.16 -2.65 0.54 0.09 -11.07 -12.27 -12.06 -5.21
Endosome -7.93 11.99 8.87 13.32 -1.86 -12.13 9.64 0.25 7.36 -1.24 -14.80 15.47 7.54 18.05 -2.79
Golgi -6.06 11.98 0.50 4.77 -2.19 -7.00 9.11 -0.89 0.43 -1.02 -8.73 13.84 -0.17 6.81 -2.35
Lipid Particles 3.35 12.40 13.04 9.50 3.04 -2.63 9.22 9.51 7.45 1.70 0.08 15.41 16.05 12.02 3.07
Mitochondria -5.31 6.46 1.96 6.18 -2.10 -7.49 8.66 5.98 8.55 -0.68 -9.43 10.44 1.66 9.06 -2.33
Mitotic Spindle -3.23 -2.58 -7.30 -6.56 -6.32 -2.55 -0.80 -1.07 -0.85 -1.02 -3.51 -2.20 -7.17 -6.40 -6.65
None -2.24 -0.71 -0.07 2.59 0.85 1.00 -3.41 0.83 0.05 3.14 -2.62 -2.41 -2.19 1.17 0.46
Nuclear Periphery -0.02 -10.21 -16.45 -15.30 -8.28 1.61 -12.63 -3.01 -4.69 6.35 0.49 -16.49 -17.30 -17.51 -8.86
Nuclear Periphery Foci -2.40 -4.02 -5.38 -3.95 -2.20 -1.48 -6.05 -3.07 -2.11 -0.75 -3.02 -7.17 -6.11 -4.39 -2.49
Nucleolus -4.05 1.74 4.83 7.66 2.66 -1.31 4.84 6.18 3.86 3.04 -4.71 4.08 6.96 8.40 2.78
Nucleus 8.96 -38.81 -16.77 -22.13 19.28 15.81 -39.09 -2.69 -5.09 5.06 16.69 -54.69 -17.21 -25.05 20.14
Peroxisomes -8.20 6.81 9.78 13.23 0.81 -10.88 10.11 -0.11 4.78 -1.01 -13.67 12.11 11.10 17.64 -0.34
Vacuole -5.25 2.16 -18.93 -15.81 -18.95 -9.87 -9.67 -4.27 -3.03 -3.17 -11.48 -6.21 -21.24 -16.47 -18.19
Vacuole Periphery -6.25 7.43 -1.11 2.09 -2.39 -7.87 7.29 -0.78 1.09 -1.16 -9.54 10.52 -1.58 2.07 -2.60
ORF
Human Ortholog
Description Protein that contains a PX domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; PX stands for Phox homology
Localization
Cell Percentages cytoplasm (42%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

YPR097W

YPR097W


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypr097w-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available