ORF
Description Protein that contains a PX domain and binds phosphoinositides; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; PX stands for Phox homology

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.98 0.96 1.0 0.99 0.99 0.96 0.91 0.92 0.88 0.7 0.73 1.0 1.0 1.0 0.95 0.95 0.94 0.77 0.83 0.61 0.9 0.84 0.82
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.09 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.06 0 0.06 0 0.11 0.16 0.16 0.27 0.47 0.49 0 0 0 0.06 0 0 0.14 0.09 0.24 0.05 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 2 0 3 5
Bud 0 1 1 2 0 0 1 5 2 11 5 0 0 5 1 1 0 0 1 0 0 7 5
Bud Neck 2 0 3 1 1 3 9 12 2 7 10 0 0 0 3 1 1 1 0 0 1 0 2
Bud Site 0 0 0 0 0 0 2 0 2 2 1 0 0 0 0 0 0
Cell Periphery 2 2 0 2 0 0 1 0 2 0 1 0 0 1 0 2 2 0 1 2 1 3 2
Cytoplasm 258 248 179 205 305 306 393 447 274 204 262 314 413 440 211 146 173 219 212 98 182 258 284
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 1 1 1 0 0 0 14 4 17 1 0 1 0 1 4
Endosome 2 0 0 0 0 0 0 0 0 1 0 0 0 0 2 1 4 4 1 2 0 3 3
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2 1 0 0 0 0
Mitochondria 2 15 2 12 7 34 69 80 85 136 176 1 0 11 14 3 2 38 22 38 10 9 15
Nucleus 0 0 0 0 2 0 2 2 1 2 0 1 1 2 1 0 2 0 0 0 0 0 0
Nuclear Periphery 0 2 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 2 1 4 8 6 7 11 0 0 0 2 1 2 4 2 2 0 1 2
Vac/Vac Membrane 2 0 0 0 0 0 2 0 0 1 1 0 0 0 2 5 2 2 4 5 1 5 5
Unique Cell Count 264 257 179 207 309 318 430 487 311 292 361 314 413 442 223 154 184 284 255 161 203 308 346
Labelled Cell Count 269 270 185 222 317 344 483 555 375 374 468 316 414 460 251 165 206 284 255 161 203 308 346


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.1 2.9 2.9 3.2 2.9 2.5 3.0 2.4 2.3 2.0 3.9 4.0 3.5 5.2 5.6 5.5 3.8 3.9 4.1
Std Deviation (1e-4) 0.8 0.4 0.7 1.5 1.7 1.4 0.8 1.5 1.2 1.1 0.8 1.2 1.0 1.1 1.8 2.3 2.4 0.8 1.6 0.9
Intensity Change (Log2) 0.03 0.19 0.02 -0.17 0.05 -0.28 -0.29 -0.51 0.46 0.5 0.3 0.86 0.97 0.95 0.43 0.46 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole