Standard name
Human Ortholog
Description Member of the NineTeen Complex (NTC); this complex contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; interacts physically and genetically with Prp19p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0.07 0.05 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0.06 0.07 – 0.09 0.06 0.06 0 0.05 0.13 0.16 0.07 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.09 0 0 0 0.25 0.1 – 0.16 0.19 0.31 0.31 0 0 0 0.1 0.06 0 0 0 0 0 0 0
Nucleus 0.96 0.94 0.95 0.93 0.92 0.83 0.85 – 0.81 0.83 0.78 0.81 0.93 0.85 0.81 0.85 0.86 0.83 0.89 0.94 0.87 0.92 0.79 0.72
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0.06 0 0 0 0 0 – 0.05 0 0.06 0 0 0 0 0.05 0.07 0.09 0 0 0 0 0.1 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud 0 0 0 0 0 4 1 – 2 1 5 7 0 0 1 0 0 0 4 0 1 3 6 8
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 1 0 3 1 – 14 8 23 20 0 1 0 0 0 0 – – – – – –
Cell Periphery 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2 1 0 8 11 12 28 – 28 21 17 15 13 67 79 24 10 22 0 0 1 0 1 1
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 1 0 0 7 5 12 0 0 0 1 0 1
Endosome 2 0 1 1 6 0 5 – 0 1 2 0 0 3 7 1 6 5 2 0 0 0 3 3
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 1 1 1 0 0 0 1 0 0 1 2 1
Mitochondria 7 24 4 6 7 48 38 – 53 72 95 113 6 6 8 35 16 10 0 0 0 0 1 7
Nucleus 270 247 94 187 250 158 333 – 265 309 241 294 226 444 412 289 229 271 250 249 99 349 416 339
Nuclear Periphery 2 0 1 7 11 1 8 – 11 9 3 6 2 6 4 3 4 5 1 0 0 0 1 4
Nucleolus 0 1 1 1 1 9 4 – 11 9 11 15 1 0 6 2 2 8 7 2 4 5 24 26
Peroxisomes 1 0 0 0 1 0 0 – 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 2 1 0 1 3 – 3 3 6 1 0 2 5 4 3 3 1 0 0 2 8 3
Vac/Vac Membrane 1 15 1 2 5 4 6 – 16 3 17 7 0 4 5 16 19 29 10 9 4 13 51 68
Unique Cell Count 280 262 99 201 272 190 390 329 373 309 365 244 520 509 341 267 327 282 266 114 380 525 470
Labelled Cell Count 286 288 104 214 292 240 427 403 436 420 479 250 534 529 381 294 365 282 266 114 380 525 470


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.9 5.6 5.9 6.3 7.2 4.7 5.5 – 5.5 5.1 5.2 4.9 6.4 6.4 6.3 6.3 6.8 7.7 6.0 6.1 6.4
Std Deviation (1e-4) 0.9 0.9 1.0 1.7 4.0 1.4 1.7 – 1.8 1.4 1.9 1.3 1.6 1.5 1.6 1.6 1.8 9.8 1.5 1.6 1.6
Intensity Change (Log2) – – – 0.11 0.29 -0.33 -0.08 – -0.09 -0.19 -0.18 -0.26 0.12 0.13 0.11 0.11 0.21 0.4 0.02 0.05 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 2.8 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 2.7 – 3.0 0 0 0 0 3.8 4.2 2.7 0 2.7
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 1.8 – 3.1 3.7 5.4 5.5 0 0 0 1.9 0.7 0
Nucleus -0.6 -1.0 -2.8 -2.6 – -3.4 -3.0 -3.8 -3.4 -0.8 -2.6 -3.4 -2.7 -2.4 -3.0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 – 0 0 0 0 0 0 0 0 2.3 2.7

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.2857 0.5989 0.2319 -0.2561 -0.367 0.2293 1.9929 2.0532 1.8763 1.4764 2.0783 1.9299 3.4946 3.6308 3.2967 3.4334 4.1618 3.6642
Actin 0 0 0 0 0.0014 0 0.0165 0 0.0012 0 0.0015 0.0007 0.0104 0 0.0003 0 0.0486 0.0159
Bud 0 0 0 0 0.0001 0 0.0004 0 0.0001 0 0.0001 0 0.0002 0 0 0 0.0038 0.0002
Bud Neck 0 0.0001 0 0.0001 0.0005 0.0003 0.0023 0 0.0001 0 0.0006 0.0007 0.0011 0.0001 0.0001 0 0.0009 0.0048
Bud Periphery 0 0 0 0 0.0001 0 0.0009 0 0.0002 0 0.0004 0.0002 0.0004 0 0.0002 0 0.0086 0.0006
Bud Site 0 0 0 0 0.0002 0 0.0009 0 0.0009 0 0.0001 0.0001 0.0011 0 0.0001 0 0.004 0.0006
Cell Periphery 0 0 0 0 0 0 0.0003 0 0.0001 0 0 0.0001 0.0001 0 0.0001 0 0.0002 0.0002
Cytoplasm 0.0009 0.0001 0.0003 0.0002 0.0015 0.0001 0.0043 0.0001 0.0024 0 0.0009 0.0004 0.001 0.0001 0.0002 0.0001 0.0196 0.0008
Cytoplasmic Foci 0 0 0 0 0.0026 0 0.0049 0 0.0015 0 0.0001 0.0015 0.0116 0 0.0006 0 0.0227 0.0045
Eisosomes 0 0 0 0 0 0 0.0002 0 0 0 0 0 0.0001 0 0.0001 0 0.0003 0.0001
Endoplasmic Reticulum 0.0001 0 0 0 0.0016 0 0.0038 0 0.0014 0 0.0011 0.0003 0.0015 0 0.0001 0 0.0011 0.0005
Endosome 0.0002 0 0 0 0.0085 0 0.0137 0 0.0042 0 0.0025 0.0031 0.007 0 0.0023 0 0.0204 0.0065
Golgi 0 0 0 0 0.001 0 0.0025 0 0.0006 0 0.0004 0.0004 0.0046 0 0.001 0 0.0235 0.0052
Lipid Particles 0 0 0 0 0.0068 0 0.007 0 0.0032 0 0.0002 0.0026 0.0062 0 0.0066 0 0.0558 0.0023
Mitochondria 0.0001 0.0001 0.0001 0.0001 0.0009 0 0.0032 0.0001 0.002 0.0001 0.0015 0.0014 0.0067 0.0001 0.0222 0.0001 0.0238 0.0037
None 0.0003 0 0.0015 0.0001 0.0014 0 0.0038 0 0.0015 0 0.0003 0.0005 0.0005 0 0.0001 0 0.0024 0.0049
Nuclear Periphery 0.0044 0.0029 0.0065 0.0027 0.0165 0.0021 0.0189 0.0019 0.0068 0.0005 0.0309 0.0083 0.0134 0.0006 0.0033 0.0007 0.0036 0.0019
Nucleolus 0.0054 0.0109 0.0056 0.0056 0.0208 0.0193 0.0086 0.0049 0.0074 0.0021 0.0299 0.0165 0.0055 0.0075 0.0049 0.0031 0.0275 0.0214
Nucleus 0.9878 0.985 0.9835 0.9898 0.9194 0.9743 0.8932 0.9926 0.9553 0.9971 0.9236 0.9553 0.9166 0.9912 0.955 0.9958 0.7014 0.9183
Peroxisomes 0 0 0 0 0.0008 0 0.0019 0 0.0017 0 0 0.0005 0.0081 0 0.0006 0 0.0206 0.0031
Punctate Nuclear 0.0005 0.0009 0.0024 0.0015 0.0146 0.0037 0.009 0.0003 0.0075 0.0001 0.003 0.0064 0.0027 0.0003 0.0011 0.0001 0.0079 0.0033
Vacuole 0.0001 0 0 0 0.0007 0 0.0025 0 0.0011 0 0.0007 0.0004 0.0006 0 0.0005 0 0.0014 0.0006
Vacuole Periphery 0.0001 0 0 0 0.0006 0 0.0014 0 0.0007 0 0.0022 0.0005 0.0009 0 0.0006 0 0.0018 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.4938 3.7363 6.9312 12.673 12.1927 2.0886 13.6943 9.5615 11.9784 14.8878
Translational Efficiency 1.8645 6.6663 3.3064 2.0739 2.0572 12.3883 1.9612 2.5402 1.9597 1.8312

Hit Data

Dataset Hit
Protein Abundance ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘