Standard name | |
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Description | Essential non-ATPase regulatory subunit of the 26S proteasome; similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Bud | 0 | 1 | 1 | 0 | – | 1 | 3 | 2 | 5 | 3 | 3 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 3 | 2 | 3 |
Bud Neck | 0 | 0 | 0 | 0 | – | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 1 | 0 | – | 10 | 11 | 9 | 12 | 11 | 13 | 15 | 0 | 1 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 5 | 6 | 8 | 7 | – | 11 | 6 | 22 | 5 | 10 | 6 | 12 | 27 | 59 | 75 | 10 | 4 | 0 | 1 | 1 | 1 | 4 | 3 | 2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 0 | 1 | 1 | 0 | 1 | 2 |
Endosome | 2 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 6 | 0 | 0 | 1 | 4 | 1 | 4 | 3 | 6 | 6 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 4 | 0 | 4 | 1 | 2 | 2 |
Mitochondria | 5 | 4 | 7 | 4 | – | 88 | 129 | 78 | 95 | 156 | 160 | 130 | 4 | 0 | 2 | 4 | 2 | 1 | 1 | 3 | 2 | 0 | 2 | 7 |
Nucleus | 279 | 289 | 385 | 239 | – | 353 | 490 | 462 | 329 | 465 | 382 | 394 | 290 | 441 | 414 | 72 | 73 | 38 | 263 | 278 | 380 | 274 | 278 | 310 |
Nuclear Periphery | 16 | 1 | 2 | 6 | – | 10 | 16 | 12 | 7 | 5 | 5 | 3 | 2 | 5 | 4 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 1 | 3 |
Nucleolus | 2 | 1 | 30 | 23 | – | 92 | 117 | 114 | 104 | 191 | 155 | 163 | 5 | 11 | 25 | 3 | 4 | 5 | 2 | 0 | 21 | 2 | 17 | 23 |
Peroxisomes | 1 | 1 | 1 | 0 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 7 | 8 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 5 |
Vac/Vac Membrane | 15 | 20 | 9 | 3 | – | 13 | 13 | 14 | 11 | 14 | 12 | 12 | 1 | 18 | 11 | 3 | 4 | 1 | 23 | 17 | 29 | 25 | 47 | 91 |
Unique Cell Count | 298 | 305 | 419 | 250 | 460 | 621 | 588 | 419 | 635 | 518 | 546 | 320 | 517 | 518 | 85 | 79 | 42 | 306 | 311 | 452 | 320 | 369 | 464 | |
Labelled Cell Count | 325 | 324 | 445 | 282 | 580 | 786 | 714 | 572 | 855 | 737 | 731 | 329 | 544 | 550 | 93 | 91 | 48 | 306 | 311 | 452 | 320 | 369 | 464 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 7.2 | 8.3 | 8.5 | 7.5 | – | 6.5 | 6.2 | 6.9 | 6.1 | 6.1 | 5.5 | 6.0 | 9.5 | 9.2 | 8.6 | 10.7 | 10.9 | 9.8 | 10.0 | 9.9 | 10.3 |
Std Deviation (1e-4) | 1.3 | 1.6 | 2.5 | 2.6 | – | 2.6 | 2.0 | 2.4 | 2.1 | 2.3 | 2.3 | 2.1 | 2.8 | 2.6 | 2.4 | 3.9 | 3.2 | 3.4 | 2.3 | 2.2 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.17 | – | -0.37 | -0.45 | -0.3 | -0.47 | -0.48 | -0.63 | -0.49 | 0.17 | 0.12 | 0.02 | 0.34 | 0.37 | 0.21 | 0.24 | 0.22 | 0.29 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | – | 2.6 | 0 | 0 | 3.1 | 0 | 2.8 | 3.0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 0.8 | – | 0.5 | -1.3 | 1.7 | -0.8 | -0.4 | -0.9 | 0.3 | 4.1 | 5.6 | 6.7 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | – | 8.3 | 9.0 | 6.5 | 9.3 | 10.1 | 11.6 | 9.8 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 1.9 | – | -6.1 | -5.6 | -5.7 | -5.4 | -7.5 | -7.2 | -7.7 | -0.6 | -3.1 | -5.1 | -2.1 | 0.2 | -0.3 |
Nuclear Periphery | 0 | – | 2.2 | 2.5 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0.9 | – | 5.5 | 5.3 | 5.5 | 7.0 | 8.9 | 8.7 | 8.7 | -3.5 | -3.7 | -1.5 | -1.2 | -0.7 | 0 |
Peroxisomes | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | – | 0.6 | -0.1 | 0.2 | 0.5 | 0.1 | 0.2 | 0.1 | 0 | 1.2 | 0 | 0 | 0 | 0 |