Standard name
Human Ortholog
Description Essential non-ATPase regulatory subunit of the 26S proteasome; similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.11 0.14 0.12 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0.19 0.21 0.13 0.23 0.25 0.31 0.24 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.94 0.95 0.92 0.96 – 0.77 0.79 0.79 0.79 0.73 0.74 0.72 0.91 0.85 0.8 0.85 0.92 0.9 0.86 0.9 0.84 0.86 0.76 0.67
Nuclear Periphery 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.07 0.09 – 0.2 0.19 0.19 0.25 0.3 0.3 0.3 0 0 0.05 0 0.05 0.12 0 0 0.05 0 0.05 0.05
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.07 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0.06 0.07 0.08 0.13 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 1 1 0 – 1 3 2 5 3 3 2 0 0 0 0 0 0 1 3 2 3 2 3
Bud Neck 0 0 0 0 – 1 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 – 10 11 9 12 11 13 15 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 6 8 7 – 11 6 22 5 10 6 12 27 59 75 10 4 0 1 1 1 4 3 2
Endoplasmic Reticulum 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1 0 1 2
Endosome 2 0 1 0 – 0 0 0 0 0 0 0 0 1 6 0 0 1 4 1 4 3 6 6
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 4 0 4 1 2 2
Mitochondria 5 4 7 4 – 88 129 78 95 156 160 130 4 0 2 4 2 1 1 3 2 0 2 7
Nucleus 279 289 385 239 – 353 490 462 329 465 382 394 290 441 414 72 73 38 263 278 380 274 278 310
Nuclear Periphery 16 1 2 6 – 10 16 12 7 5 5 3 2 5 4 0 2 1 0 0 0 0 1 3
Nucleolus 2 1 30 23 – 92 117 114 104 191 155 163 5 11 25 3 4 5 2 0 21 2 17 23
Peroxisomes 1 1 1 0 – 0 0 0 1 0 0 0 0 1 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 – 0 0 0 1 0 1 0 0 7 8 0 0 0 1 0 1 0 1 5
Vac/Vac Membrane 15 20 9 3 – 13 13 14 11 14 12 12 1 18 11 3 4 1 23 17 29 25 47 91
Unique Cell Count 298 305 419 250 460 621 588 419 635 518 546 320 517 518 85 79 42 306 311 452 320 369 464
Labelled Cell Count 325 324 445 282 580 786 714 572 855 737 731 329 544 550 93 91 48 306 311 452 320 369 464


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 8.3 8.5 7.5 – 6.5 6.2 6.9 6.1 6.1 5.5 6.0 9.5 9.2 8.6 10.7 10.9 9.8 10.0 9.9 10.3
Std Deviation (1e-4) 1.3 1.6 2.5 2.6 – 2.6 2.0 2.4 2.1 2.3 2.3 2.1 2.8 2.6 2.4 3.9 3.2 3.4 2.3 2.2 2.3
Intensity Change (Log2) – – – -0.17 – -0.37 -0.45 -0.3 -0.47 -0.48 -0.63 -0.49 0.17 0.12 0.02 0.34 0.37 0.21 0.24 0.22 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 2.6 0 0 3.1 0 2.8 3.0 0 0 0 0 0 0
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 – 0.5 -1.3 1.7 -0.8 -0.4 -0.9 0.3 4.1 5.6 6.7 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 8.3 9.0 6.5 9.3 10.1 11.6 9.8 0 0 0 0 0 0
Nucleus 1.9 – -6.1 -5.6 -5.7 -5.4 -7.5 -7.2 -7.7 -0.6 -3.1 -5.1 -2.1 0.2 -0.3
Nuclear Periphery 0 – 2.2 2.5 2.1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.9 – 5.5 5.3 5.5 7.0 8.9 8.7 8.7 -3.5 -3.7 -1.5 -1.2 -0.7 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 – 0.6 -0.1 0.2 0.5 0.1 0.2 0.1 0 1.2 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.9066 6.1124 5.6127 5.4527 5.0005 5.7047 3.5387 4.2174 3.5699 3.7081 3.0751 3.4656 4.6926 4.983 4.5921 4.2562 3.9676 4.4471
Actin 0.0021 0 0.0178 0.0016 0.0285 0 0.0181 0.0047 0.0171 0 0.0003 0.0169 0.0109 0 0.0077 0.0206 0.001 0.001
Bud 0.0001 0 0.0006 0.0002 0.0005 0 0.0003 0.0002 0.0006 0 0.0003 0.0002 0.0008 0 0 0.0011 0.0001 0.0001
Bud Neck 0.0045 0.0001 0.0008 0.0008 0.0005 0.0006 0.0013 0.0004 0.0008 0 0.0002 0.001 0.0007 0 0.0001 0.0003 0.0004 0.0013
Bud Periphery 0.0004 0 0.0011 0.0006 0.0011 0.0001 0.0005 0.0004 0.0008 0 0.0012 0.0005 0.0017 0 0.0001 0.0054 0.0003 0.0002
Bud Site 0.0003 0 0.0018 0.0015 0.0023 0 0.0019 0.0014 0.0011 0 0.0003 0.0003 0.0029 0 0.0003 0.0009 0.0003 0.0001
Cell Periphery 0.0002 0 0.0004 0.0008 0.0002 0 0.0002 0.0001 0.0002 0 0.0004 0.0001 0.0008 0 0.0001 0.0002 0.0001 0.0001
Cytoplasm 0.001 0.0008 0.0018 0.0044 0.0019 0.0001 0.0069 0.0005 0.0029 0.0001 0.0003 0.0003 0.0018 0.0001 0.0014 0.0004 0.0007 0.0008
Cytoplasmic Foci 0.0031 0 0.0028 0.0002 0.0044 0 0.0371 0.0004 0.0079 0 0.0006 0.0062 0.0064 0 0.0002 0.0007 0.0067 0.0003
Eisosomes 0 0 0.0005 0.0001 0.0002 0 0.0002 0 0.0001 0 0 0.0002 0.0002 0 0 0.0004 0 0
Endoplasmic Reticulum 0.0037 0.0001 0.002 0.0065 0.0005 0 0.0036 0.0005 0.002 0 0.0005 0.0007 0.0053 0 0.0007 0.0017 0.0015 0.001
Endosome 0.0145 0.0001 0.0036 0.0011 0.0031 0.0001 0.025 0.0016 0.0177 0 0.0074 0.0243 0.0087 0 0.0026 0.0047 0.0268 0.0034
Golgi 0.0023 0 0.0008 0.0001 0.0055 0 0.0164 0.0008 0.0144 0 0.0013 0.0235 0.0021 0 0.0002 0.0021 0.0131 0.0027
Lipid Particles 0.0081 0 0.0029 0.0005 0.0135 0 0.0394 0.0007 0.0063 0 0.0034 0.017 0.0075 0 0.0002 0.003 0.0131 0.0009
Mitochondria 0.0019 0.0002 0.0024 0.002 0.031 0.0002 0.0062 0.0044 0.0028 0.0003 0.0595 0.0124 0.02 0.0002 0.0008 0.0469 0.0048 0.0023
None 0.0012 0.0002 0.0022 0.0006 0.0069 0 0.0022 0 0.0005 0 0.0001 0.0002 0.0011 0 0.0001 0.0005 0.0003 0.0001
Nuclear Periphery 0.0164 0.0033 0.0079 0.0203 0.0096 0.0014 0.014 0.0049 0.0083 0.0036 0.003 0.0172 0.0289 0.0041 0.0157 0.0064 0.0327 0.0072
Nucleolus 0.0045 0.0038 0.0033 0.0081 0.0185 0.0131 0.0071 0.003 0.0027 0.0024 0.0081 0.0073 0.0173 0.0033 0.0018 0.003 0.0147 0.0095
Nucleus 0.9287 0.9873 0.9422 0.9438 0.8383 0.9832 0.7767 0.9743 0.8978 0.9933 0.8891 0.8642 0.8617 0.9921 0.9617 0.8952 0.8558 0.9663
Peroxisomes 0.0017 0 0.0006 0.0001 0.0106 0 0.0274 0.0003 0.0032 0 0.0005 0.004 0.0063 0 0.0002 0.0008 0.0153 0.0001
Punctate Nuclear 0.0019 0.0038 0.003 0.0031 0.0167 0.0006 0.0113 0.0001 0.0068 0 0.0001 0.0005 0.0054 0.0001 0.0013 0.0003 0.0039 0.0005
Vacuole 0.0017 0.0001 0.0011 0.0027 0.0027 0.0003 0.0027 0.0008 0.005 0.0001 0.0152 0.001 0.0056 0.0001 0.004 0.0028 0.0054 0.0014
Vacuole Periphery 0.0016 0.0001 0.0003 0.0009 0.0036 0.0001 0.0014 0.0005 0.0011 0.0002 0.0084 0.0021 0.0038 0.0001 0.0007 0.0026 0.0028 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 85.9629 77.2279 76.5977 107.1231 61.5496 79.5037 94.5138 106.9697 107.589 92.0865
Translational Efficiency 2.453 2.236 2.4893 1.8321 2.2305 2.7867 2.2223 1.7396 1.8578 1.6927

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘