Standard name
Description Essential non-ATPase regulatory subunit of the 26S proteasome; similar to another S. cerevisiae regulatory subunit, Rpn5p, as well as to mammalian proteasome subunits
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 – 0 0 0 0 0 0 0 0.08 0.11 0.14 0.12 0.05 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0.19 0.21 0.13 0.23 0.25 0.31 0.24 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.94 0.95 0.92 0.96 – 0.77 0.79 0.79 0.79 0.73 0.74 0.72 0.91 0.85 0.8 0.85 0.92 0.9 0.86 0.9 0.84 0.86 0.76 0.67
Nuclear Periphery 0.05 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.07 0.09 – 0.2 0.19 0.19 0.25 0.3 0.3 0.3 0 0 0.05 0 0.05 0.12 0 0 0.05 0 0.05 0.05
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.05 0.07 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.08 0.06 0.07 0.08 0.13 0.2
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1
Bud 0 1 1 0 – 1 3 2 5 3 3 2 0 0 0 0 0 0 1 3 2 3 2 3
Bud Neck 0 0 0 0 – 1 1 1 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 1 0 – 10 11 9 12 11 13 15 0 1 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 5 6 8 7 – 11 6 22 5 10 6 12 27 59 75 10 4 0 1 1 1 4 3 2
Endoplasmic Reticulum 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 2 1 0 1 1 0 1 2
Endosome 2 0 1 0 – 0 0 0 0 0 0 0 0 1 6 0 0 1 4 1 4 3 6 6
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 1 0 0 4 0 4 1 2 2
Mitochondria 5 4 7 4 – 88 129 78 95 156 160 130 4 0 2 4 2 1 1 3 2 0 2 7
Nucleus 279 289 385 239 – 353 490 462 329 465 382 394 290 441 414 72 73 38 263 278 380 274 278 310
Nuclear Periphery 16 1 2 6 – 10 16 12 7 5 5 3 2 5 4 0 2 1 0 0 0 0 1 3
Nucleolus 2 1 30 23 – 92 117 114 104 191 155 163 5 11 25 3 4 5 2 0 21 2 17 23
Peroxisomes 1 1 1 0 – 0 0 0 1 0 0 0 0 1 4 0 0 0 0 0 0 0 0 0
SpindlePole 0 1 0 0 – 0 0 0 1 0 1 0 0 7 8 0 0 0 1 0 1 0 1 5
Vac/Vac Membrane 15 20 9 3 – 13 13 14 11 14 12 12 1 18 11 3 4 1 23 17 29 25 47 91
Unique Cell Count 298 305 419 250 460 621 588 419 635 518 546 320 517 518 85 79 42 306 311 452 320 369 464
Labelled Cell Count 325 324 445 282 580 786 714 572 855 737 731 329 544 550 93 91 48 306 311 452 320 369 464


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 8.3 8.5 7.5 – 6.5 6.2 6.9 6.1 6.1 5.5 6.0 9.5 9.2 8.6 10.7 10.9 9.8 10.0 9.9 10.3
Std Deviation (1e-4) 1.3 1.6 2.5 2.6 – 2.6 2.0 2.4 2.1 2.3 2.3 2.1 2.8 2.6 2.4 3.9 3.2 3.4 2.3 2.2 2.3
Intensity Change (Log2) – – – -0.17 – -0.37 -0.45 -0.3 -0.47 -0.48 -0.63 -0.49 0.17 0.12 0.02 0.34 0.37 0.21 0.24 0.22 0.29


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 – 2.6 0 0 3.1 0 2.8 3.0 0 0 0 0 0 0
Cell Periphery 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.8 – 0.5 -1.3 1.7 -0.8 -0.4 -0.9 0.3 4.1 5.6 6.7 0 0 0
Endoplasmic Reticulum 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 – 8.3 9.0 6.5 9.3 10.1 11.6 9.8 0 0 0 0 0 0
Nucleus 1.9 – -6.1 -5.6 -5.7 -5.4 -7.5 -7.2 -7.7 -0.6 -3.1 -5.1 -2.1 0.2 -0.3
Nuclear Periphery 0 – 2.2 2.5 2.1 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.9 – 5.5 5.3 5.5 7.0 8.9 8.7 8.7 -3.5 -3.7 -1.5 -1.2 -0.7 0
Peroxisomes 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 – 0.6 -0.1 0.2 0.5 0.1 0.2 0.1 0 1.2 0 0 0 0