Standard name | |
---|---|
Human Ortholog | |
Description | Negative regulator of actin nucleation-promoting factor activity; interacts with Las17p, a homolog of human Wiskott-Aldrich Syndrome protein (WASP), via an N-terminal SH3 domain, and along with LSB1 cooperatively inhibits the nucleation of actin filaments; short-lived protein whose levels increase in response to thermal stress; induces the formation of the [PIN+] and [RNQ+] prions when overproduced; PIN3 has a paralog, LSB1, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 2 | 2 |
Bud | 0 | 0 | 0 | 0 | 0 | 3 | 0 | 1 | 1 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 2 | 8 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 5 | 2 | 3 | 1 | 3 | 8 | 2 | 7 | 8 | 7 | 1 | 1 | 2 | 4 | 4 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 161 | 330 | 188 | 185 | 269 | 138 | 209 | 244 | 174 | 203 | 149 | 140 | 315 | 407 | 422 | 178 | 179 | 175 | 161 | 310 | 182 | 308 | 341 | 378 |
Endoplasmic Reticulum | 1 | 6 | 2 | 1 | 3 | 1 | 2 | 1 | 1 | 4 | 4 | 7 | 6 | 0 | 1 | 5 | 2 | 5 | 1 | 2 | 0 | 1 | 6 | 8 |
Endosome | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 3 | 1 | 0 | 3 | 14 |
Golgi | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 | 2 | 1 | 0 | 1 | 2 | 2 |
Mitochondria | 3 | 1 | 0 | 3 | 1 | 18 | 6 | 17 | 10 | 27 | 21 | 26 | 1 | 0 | 0 | 7 | 3 | 2 | 1 | 3 | 0 | 0 | 1 | 0 |
Nucleus | 0 | 2 | 1 | 1 | 1 | 4 | 4 | 6 | 3 | 13 | 6 | 9 | 4 | 1 | 0 | 4 | 5 | 3 | 0 | 3 | 2 | 2 | 2 | 4 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 4 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 4 |
Vac/Vac Membrane | 4 | 1 | 5 | 17 | 81 | 12 | 73 | 112 | 50 | 61 | 35 | 40 | 3 | 18 | 20 | 38 | 19 | 17 | 2 | 4 | 0 | 4 | 3 | 4 |
Unique Cell Count | 169 | 338 | 194 | 205 | 323 | 166 | 267 | 366 | 233 | 290 | 208 | 206 | 322 | 418 | 437 | 211 | 200 | 190 | 179 | 340 | 194 | 327 | 373 | 441 |
Labelled Cell Count | 170 | 342 | 202 | 209 | 358 | 178 | 297 | 391 | 242 | 319 | 227 | 233 | 330 | 427 | 445 | 241 | 215 | 207 | 179 | 340 | 194 | 327 | 373 | 441 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.7 | 9.8 | 9.9 | 9.5 | 11.1 | 7.1 | 9.9 | 9.7 | 9.0 | 9.0 | 9.0 | 8.6 | 9.3 | 10.6 | 10.9 | 16.8 | 16.2 | 15.2 | 8.8 | 10.1 | 11.1 |
Std Deviation (1e-4) | 1.2 | 1.8 | 1.7 | 1.5 | 1.7 | 1.7 | 2.2 | 1.9 | 2.3 | 2.1 | 2.4 | 2.0 | 1.9 | 2.0 | 1.9 | 6.1 | 5.2 | 5.8 | 1.8 | 2.0 | 2.3 |
Intensity Change (Log2) | – | – | – | -0.07 | 0.16 | -0.49 | -0.01 | -0.04 | -0.15 | -0.15 | -0.15 | -0.2 | -0.09 | 0.1 | 0.14 | 0.75 | 0.71 | 0.61 | -0.17 | 0.02 | 0.16 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0.7 | 0.5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -2.7 | -4.7 | -4.5 | -5.7 | -8.1 | -6.4 | -7.4 | -6.9 | -7.5 | 0.6 | 0.3 | -0.2 | -4.3 | -2.9 | -2.1 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 4.7 | 0 | 3.0 | 0 | 4.4 | 4.5 | 5.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 2.5 | 6.7 | 2.1 | 7.0 | 7.8 | 5.8 | 5.8 | 4.8 | 5.3 | 0 | 1.0 | 1.2 | 5.0 | 2.9 | 2.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.8839 | 5.3711 | 4.1951 | 3.4719 | 3.591 | 4.1487 | 5.5378 | 7.1806 | 6.1087 | 5.1042 | 5.767 | 6.1453 | 7.9033 | 9.8486 | 8.5967 | 8.8534 | 8.211 | 8.5948 |
Actin | 0.0336 | 0.0024 | 0.0218 | 0.0323 | 0.0302 | 0.0059 | 0.0324 | 0.0035 | 0.0285 | 0.0849 | 0.046 | 0.0057 | 0.0594 | 0.0012 | 0.0276 | 0.0027 | 0.0256 | 0.0034 |
Bud | 0.0009 | 0.0131 | 0.0036 | 0.0012 | 0.002 | 0.001 | 0.0011 | 0.0001 | 0.0021 | 0.0015 | 0.0003 | 0.0003 | 0.0008 | 0.0001 | 0.0007 | 0.0005 | 0.0015 | 0.0001 |
Bud Neck | 0.0051 | 0.0006 | 0.0023 | 0.0025 | 0.0048 | 0.0035 | 0.0025 | 0.0003 | 0.0022 | 0.002 | 0.0011 | 0.0019 | 0.008 | 0.0003 | 0.0049 | 0.0009 | 0.0011 | 0.0018 |
Bud Periphery | 0.0008 | 0.0028 | 0.0015 | 0.0016 | 0.0022 | 0.0005 | 0.0021 | 0 | 0.0022 | 0.002 | 0.0004 | 0.0002 | 0.0014 | 0 | 0.0012 | 0.0002 | 0.0013 | 0.0001 |
Bud Site | 0.0035 | 0.0119 | 0.0084 | 0.0024 | 0.0048 | 0.0002 | 0.0027 | 0.0017 | 0.0164 | 0.0013 | 0.0009 | 0.0002 | 0.0079 | 0.0005 | 0.0059 | 0.0008 | 0.0004 | 0.0001 |
Cell Periphery | 0.0002 | 0.0003 | 0.0005 | 0.0003 | 0.0003 | 0.0001 | 0.0002 | 0.0001 | 0.0008 | 0.0002 | 0.0001 | 0 | 0.0012 | 0.0001 | 0.0012 | 0.0001 | 0.0001 | 0.0001 |
Cytoplasm | 0.7266 | 0.911 | 0.8656 | 0.808 | 0.7237 | 0.9002 | 0.8279 | 0.9776 | 0.8553 | 0.8541 | 0.8743 | 0.9126 | 0.8438 | 0.9823 | 0.9048 | 0.9556 | 0.895 | 0.9443 |
Cytoplasmic Foci | 0.0425 | 0.0138 | 0.0203 | 0.016 | 0.0285 | 0.0231 | 0.0271 | 0.0036 | 0.01 | 0.0027 | 0.0094 | 0.0125 | 0.005 | 0.0015 | 0.0042 | 0.0078 | 0.0034 | 0.0026 |
Eisosomes | 0.0003 | 0 | 0.0005 | 0.0003 | 0.0013 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0003 | 0.0002 | 0 | 0.0007 | 0 | 0.0009 | 0 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.008 | 0.0015 | 0.005 | 0.0048 | 0.0061 | 0.0033 | 0.0025 | 0.0011 | 0.003 | 0.0017 | 0.0062 | 0.0027 | 0.007 | 0.0012 | 0.0038 | 0.0025 | 0.0065 | 0.0035 |
Endosome | 0.0472 | 0.0015 | 0.0103 | 0.019 | 0.0471 | 0.0104 | 0.0223 | 0.0006 | 0.0163 | 0.007 | 0.0063 | 0.0105 | 0.008 | 0.0002 | 0.003 | 0.0026 | 0.0117 | 0.003 |
Golgi | 0.015 | 0.0003 | 0.0036 | 0.0178 | 0.0178 | 0.0018 | 0.0122 | 0.0003 | 0.0139 | 0.0157 | 0.004 | 0.0058 | 0.0031 | 0.0001 | 0.0018 | 0.0004 | 0.0021 | 0.0008 |
Lipid Particles | 0.0164 | 0.0001 | 0.0025 | 0.0058 | 0.0129 | 0.0016 | 0.009 | 0.0007 | 0.0057 | 0.0019 | 0.0096 | 0.0041 | 0.0013 | 0 | 0.0021 | 0.0004 | 0.0016 | 0.0005 |
Mitochondria | 0.0056 | 0.0006 | 0.0052 | 0.0212 | 0.0113 | 0.0035 | 0.0104 | 0.0002 | 0.0135 | 0.0075 | 0.0011 | 0.0048 | 0.0015 | 0.0001 | 0.0012 | 0.0002 | 0.0031 | 0.0009 |
None | 0.0248 | 0.015 | 0.0169 | 0.032 | 0.0347 | 0.0115 | 0.0167 | 0.0052 | 0.0074 | 0.0035 | 0.0057 | 0.0127 | 0.0245 | 0.0059 | 0.0263 | 0.0042 | 0.0078 | 0.0152 |
Nuclear Periphery | 0.0119 | 0.0016 | 0.0032 | 0.0076 | 0.0128 | 0.0067 | 0.0029 | 0.0006 | 0.0016 | 0.0011 | 0.0025 | 0.0016 | 0.0083 | 0.0007 | 0.0018 | 0.0028 | 0.021 | 0.0054 |
Nucleolus | 0.0005 | 0.0003 | 0.0005 | 0.0004 | 0.003 | 0.0001 | 0.0003 | 0 | 0.0024 | 0.0001 | 0.0002 | 0 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0005 | 0.0007 |
Nucleus | 0.0214 | 0.0172 | 0.0163 | 0.0169 | 0.0244 | 0.0149 | 0.015 | 0.0032 | 0.0103 | 0.0099 | 0.0053 | 0.009 | 0.0083 | 0.0052 | 0.0057 | 0.0104 | 0.0086 | 0.0146 |
Peroxisomes | 0.0223 | 0.0032 | 0.0057 | 0.002 | 0.0073 | 0.0041 | 0.007 | 0.0004 | 0.0031 | 0.0006 | 0.0192 | 0.0137 | 0.0008 | 0 | 0.0005 | 0.0002 | 0.0005 | 0.0001 |
Punctate Nuclear | 0.0096 | 0.002 | 0.0042 | 0.0057 | 0.0198 | 0.0058 | 0.0025 | 0.0003 | 0.0019 | 0.0005 | 0.006 | 0.001 | 0.0073 | 0.0003 | 0.001 | 0.0071 | 0.0063 | 0.002 |
Vacuole | 0.0023 | 0.0007 | 0.0017 | 0.0015 | 0.0034 | 0.0014 | 0.0018 | 0.0003 | 0.0021 | 0.0008 | 0.0009 | 0.0003 | 0.0008 | 0.0002 | 0.0009 | 0.0005 | 0.0009 | 0.0004 |
Vacuole Periphery | 0.0014 | 0.0001 | 0.0004 | 0.0009 | 0.0015 | 0.0004 | 0.001 | 0 | 0.0009 | 0.0006 | 0.0002 | 0.0003 | 0.0005 | 0 | 0.0002 | 0.0001 | 0.0008 | 0.0004 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 40.8668 | 24.355 | 33.8858 | 46.7354 | 26.4928 | 44.2479 | 50.7896 | 59.2102 | 44.463 | 47.7991 |
Translational Efficiency | 2.184 | 3.1075 | 1.8488 | 1.0241 | 2.5955 | 2.958 | 1.9678 | 1.8235 | 2.3387 | 1.8345 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
225 | 1342 | 832 | 1873 | 1552 | 2095 | 181 | 2219 | 1777 | 3437 | 1013 | 4092 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 1472.09 | 1079.34 | 1372.93 | 1437.41 | 944.86 | 1092.65 | 1521.46 | 1640.33 | 1011.62 | 1087.45 | 1399.47 | 1547.45 |
Standard Deviation | 334.13 | 183.66 | 213.49 | 320.08 | 132.44 | 170.45 | 218.11 | 382.01 | 245.35 | 175.85 | 221.75 | 369.12 |
Intensity Change Log 2 | — | -0.447716 | -0.100608 | -0.034394 | — | 0.209659 | 0.687284 | 0.795814 | — | -0.154170 | 0.260072 | 0.348684 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.001809 | 0.000249 | 0.000783 | 0.002416 | 0.000218 | 0.000468 | 0.001613 | 0.003401 | 0.000419 | 0.000382 | 0.000931 | 0.002950 |
Bud Neck | 0.004299 | 0.002399 | 0.004341 | 0.005266 | 0.003270 | 0.002441 | 0.006303 | 0.007474 | 0.003400 | 0.002425 | 0.004691 | 0.006463 |
Bud Site | 0.034292 | 0.006364 | 0.014107 | 0.033238 | 0.008941 | 0.009149 | 0.017969 | 0.041481 | 0.012151 | 0.008061 | 0.014797 | 0.037708 |
Cell Periphery | 0.000596 | 0.000087 | 0.000264 | 0.000308 | 0.000121 | 0.000079 | 0.000515 | 0.000184 | 0.000181 | 0.000082 | 0.000309 | 0.000241 |
Cytoplasm | 0.684721* | 0.795575* | 0.553074* | 0.636301* | 0.711533* | 0.767248* | 0.427772* | 0.647241* | 0.708138* | 0.778309* | 0.530686* | 0.642233* |
Cytoplasmic Foci | 0.106074 | 0.047766 | 0.015155 | 0.016301 | 0.097972 | 0.045857 | 0.025699 | 0.023667 | 0.098998 | 0.046602 | 0.017039 | 0.020296 |
Eisosomes | 0.000025 | 0.000014 | 0.000028 | 0.000018 | 0.000040 | 0.000023 | 0.000050 | 0.000014 | 0.000038 | 0.000019 | 0.000032 | 0.000016 |
Endoplasmic Reticulum | 0.011332 | 0.000991 | 0.003555 | 0.000896 | 0.001483 | 0.000758 | 0.003908 | 0.000393 | 0.002730 | 0.000849 | 0.003619 | 0.000623 |
Endosome | 0.006379 | 0.002053 | 0.004408 | 0.002103 | 0.004866 | 0.001921 | 0.003791 | 0.004891 | 0.005057 | 0.001972 | 0.004298 | 0.003615 |
Golgi | 0.004258 | 0.000484 | 0.000307 | 0.004928 | 0.000854 | 0.001244 | 0.000325 | 0.002941 | 0.001285 | 0.000947 | 0.000310 | 0.003850 |
Lipid Particles | 0.010584 | 0.000575 | 0.002311 | 0.001944 | 0.001511 | 0.000699 | 0.003708 | 0.001411 | 0.002660 | 0.000651 | 0.002561 | 0.001655 |
Mitochondria | 0.001961 | 0.000444 | 0.000431 | 0.001126 | 0.001356 | 0.000282 | 0.000394 | 0.001524 | 0.001432 | 0.000345 | 0.000424 | 0.001341 |
Mitotic Spindle | 0.012054 | 0.004066 | 0.024230 | 0.033548 | 0.004112 | 0.004940 | 0.006806 | 0.069335 | 0.005118 | 0.004599 | 0.021117 | 0.052954 |
None | 0.001359 | 0.006641 | 0.006710 | 0.007664 | 0.003127 | 0.003572 | 0.002487 | 0.009098 | 0.002903 | 0.004770 | 0.005956 | 0.008442 |
Nuclear Periphery | 0.000518 | 0.000148 | 0.001536 | 0.000374 | 0.000206 | 0.000179 | 0.001464 | 0.000544 | 0.000245 | 0.000167 | 0.001523 | 0.000466 |
Nuclear Periphery Foci | 0.012611 | 0.003332 | 0.008587 | 0.003691 | 0.002446 | 0.001307 | 0.010982 | 0.001541 | 0.003733 | 0.002098 | 0.009015 | 0.002525 |
Nucleolus | 0.000276 | 0.000477 | 0.000278 | 0.000161 | 0.000224 | 0.000541 | 0.000182 | 0.000351 | 0.000230 | 0.000516 | 0.000261 | 0.000264 |
Nucleus | 0.063282 | 0.091627 | 0.213503* | 0.195353* | 0.088968 | 0.109517 | 0.246198* | 0.127637 | 0.085716 | 0.102532 | 0.219344* | 0.158632* |
Peroxisomes | 0.000831 | 0.000695 | 0.000767 | 0.001057 | 0.000987 | 0.000993 | 0.000300 | 0.002745 | 0.000967 | 0.000877 | 0.000684 | 0.001972 |
Vacuole | 0.039324 | 0.035197 | 0.145336 | 0.052035 | 0.067259 | 0.048132 | 0.239329* | 0.052106 | 0.063722 | 0.043082 | 0.162130 | 0.052074 |
Vacuole Periphery | 0.003415 | 0.000818 | 0.000289 | 0.001271 | 0.000507 | 0.000652 | 0.000204 | 0.002022 | 0.000875 | 0.000716 | 0.000273 | 0.001678 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | 6.22 | 4.32 | -0.27 | -4.87 | -3.50 | -1.88 | -8.94 | -3.78 | -2.97 | -0.44 | 0.39 | -6.58 | -5.22 | -5.17 | -3.50 |
Bud Neck | 3.06 | -0.99 | 0.24 | -5.07 | 2.14 | 0.97 | -5.74 | -5.78 | -8.68 | 1.76 | 1.92 | -5.73 | -5.17 | -9.89 | 1.59 |
Bud Site | 6.64 | 5.38 | 2.82 | -9.25 | -5.72 | -0.18 | -3.79 | -7.55 | -8.00 | -2.08 | 2.99 | -0.42 | -6.73 | -11.81 | -6.88 |
Cell Periphery | 7.32 | 4.35 | 4.02 | -12.85 | -1.02 | 6.35 | -9.84 | -8.61 | -11.72 | 6.76 | 8.79 | -7.90 | -6.15 | -17.45 | 3.19 |
Cytoplasm | -7.18 | 11.58 | 5.64 | 30.29 | -11.52 | -6.06 | 26.98 | 17.68 | 25.41 | -18.18 | -9.84 | 30.95 | 21.01 | 38.57 | -17.70 |
Cytoplasmic Foci | 6.59 | 11.00 | 10.41 | 11.86 | -3.27 | 13.70 | 21.67 | 22.67 | 12.09 | -1.93 | 15.42 | 28.37 | 26.03 | 17.12 | -5.17 |
Eisosomes | 4.17 | -1.21 | 0.66 | -5.85 | 3.03 | 5.19 | -4.88 | 12.17 | 2.09 | 10.61 | 8.35 | -0.06 | 9.37 | -1.08 | 9.57 |
Endoplasmic Reticulum | 2.32 | 1.77 | 2.36 | 0.68 | 8.58 | 3.63 | -6.06 | 6.25 | 3.71 | 9.98 | 3.29 | -1.17 | 3.89 | 2.36 | 11.98 |
Endosome | 3.37 | 2.58 | 4.32 | 3.00 | 8.04 | 7.64 | 3.63 | 7.41 | 0.57 | 3.49 | 8.35 | 5.16 | 10.12 | 2.40 | 6.95 |
Golgi | 2.94 | 3.14 | 2.06 | -1.43 | -1.88 | -0.66 | 2.93 | 0.35 | 0.85 | -1.72 | 1.01 | 4.44 | 0.73 | -0.23 | -2.56 |
Lipid Particles | 4.53 | 3.59 | 3.41 | -10.42 | -1.37 | 4.75 | -9.59 | -5.79 | -8.05 | 2.35 | 6.16 | -1.18 | -1.78 | -12.08 | -1.09 |
Mitochondria | 1.20 | 1.01 | 1.09 | -0.53 | 0.71 | 1.90 | 1.40 | 1.11 | -2.31 | -0.74 | 2.04 | 1.50 | 1.46 | -2.09 | -0.06 |
Mitotic Spindle | 2.56 | -1.17 | -2.03 | -6.61 | -0.89 | -0.38 | -1.89 | -9.83 | -9.89 | -4.00 | 0.42 | -4.67 | -10.61 | -11.86 | -3.62 |
None | -7.93 | -10.08 | -13.24 | 0.94 | -0.67 | -0.40 | -0.96 | -4.53 | -4.71 | -2.21 | -2.48 | -3.61 | -5.38 | -3.47 | -2.28 |
Nuclear Periphery | 2.28 | -10.07 | -0.97 | -20.02 | 15.97 | 0.38 | -13.76 | -10.84 | -10.81 | 9.63 | 2.57 | -22.68 | -12.86 | -17.37 | 17.35 |
Nuclear Periphery Foci | 5.40 | 3.75 | 5.57 | 0.87 | 4.55 | 4.38 | -6.48 | 1.41 | -3.73 | 6.88 | 4.93 | -5.10 | 3.55 | -1.45 | 7.92 |
Nucleolus | -1.20 | -0.90 | 0.62 | 1.71 | 4.12 | -1.33 | 0.19 | -2.33 | 0.73 | -2.61 | -1.72 | -2.08 | -1.59 | 1.33 | 0.17 |
Nucleus | -4.51 | -20.69 | -20.67 | -23.92 | 5.25 | -6.01 | -10.92 | -18.28 | -12.60 | 5.54 | -6.23 | -24.77 | -28.71 | -24.87 | 9.65 |
Peroxisomes | 1.22 | 2.12 | 1.56 | 0.61 | -1.25 | -0.11 | 2.64 | -0.89 | -0.85 | -3.89 | 0.46 | 2.83 | 0.22 | -0.30 | -3.52 |
Vacuole | 2.79 | -14.64 | -8.95 | -20.44 | 9.22 | 3.69 | -15.32 | -16.40 | -20.04 | 10.94 | 6.02 | -22.37 | -19.72 | -28.12 | 12.66 |
Vacuole Periphery | 1.34 | 1.78 | 1.69 | 0.80 | -1.00 | -0.39 | 2.76 | 0.10 | 0.41 | -1.66 | 0.39 | 2.75 | 1.56 | 0.90 | -1.77 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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