Standard name | |
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Description | Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; specific for spermine; localizes to the plasma membrane; TPO3 has a paralog, TPO2, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 51 | 119 | 65 | 52 | 62 | 67 | 96 | 114 | 79 | 117 | 80 | 107 | 119 | 74 | 68 | 29 | 17 | 22 | 5 | 11 | 9 | 9 | 13 | 8 |
Bud | 15 | 13 | 10 | 8 | 7 | 14 | 13 | 38 | 34 | 15 | 24 | 21 | 14 | 8 | 8 | 4 | 6 | 2 | 4 | 3 | 0 | 1 | 3 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 4 | 2 | 1 | 4 | 6 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 229 | 316 | 179 | 120 | 141 | 125 | 168 | 198 | 165 | 183 | 177 | 187 | 537 | 601 | 651 | 245 | 194 | 180 | 220 | 349 | 204 | 251 | 351 | 344 |
Cytoplasm | 46 | 44 | 28 | 53 | 55 | 104 | 95 | 133 | 102 | 93 | 81 | 98 | 119 | 120 | 142 | 65 | 62 | 74 | 14 | 13 | 4 | 6 | 11 | 10 |
Endoplasmic Reticulum | 61 | 64 | 58 | 38 | 80 | 80 | 69 | 72 | 20 | 38 | 23 | 35 | 99 | 156 | 111 | 5 | 8 | 4 | 92 | 81 | 60 | 65 | 42 | 26 |
Endosome | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 |
Golgi | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 3 | 0 | 0 | 2 | 0 | 0 | 0 | 1 | 1 |
Mitochondria | 8 | 12 | 13 | 29 | 20 | 64 | 75 | 129 | 106 | 126 | 144 | 173 | 2 | 0 | 0 | 33 | 23 | 21 | 2 | 5 | 4 | 3 | 1 | 6 |
Nucleus | 0 | 0 | 1 | 0 | 2 | 0 | 1 | 1 | 0 | 1 | 1 | 3 | 1 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 4 | 3 | 6 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 1 | 0 | 1 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 3 | 1 | 4 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 1 | 3 | 6 | 0 | 3 | 4 | 0 | 4 | 2 | 1 | 1 | 2 | 2 |
Unique Cell Count | 313 | 401 | 252 | 196 | 251 | 313 | 352 | 468 | 321 | 363 | 342 | 380 | 690 | 784 | 813 | 321 | 270 | 258 | 354 | 477 | 290 | 345 | 439 | 415 |
Labelled Cell Count | 410 | 570 | 357 | 301 | 368 | 456 | 518 | 693 | 506 | 579 | 535 | 633 | 901 | 966 | 986 | 390 | 316 | 306 | 354 | 477 | 290 | 345 | 439 | 415 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 14.1 | 16.5 | 11.8 | 10.8 | 11.1 | 10.0 | 10.4 | 10.6 | 10.1 | 9.5 | 10.0 | 9.8 | 13.6 | 13.7 | 13.9 | 11.5 | 13.1 | 15.0 | 13.7 | 12.9 | 12.4 |
Std Deviation (1e-4) | 3.3 | 4.4 | 3.0 | 2.8 | 2.5 | 2.5 | 2.3 | 2.4 | 2.1 | 1.9 | 2.2 | 2.1 | 3.1 | 2.7 | 2.7 | 2.3 | 3.2 | 3.3 | 3.2 | 2.5 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.13 | -0.08 | -0.24 | -0.18 | -0.15 | -0.22 | -0.32 | -0.24 | -0.26 | 0.21 | 0.21 | 0.24 | -0.03 | 0.15 | 0.34 | 0.21 | 0.13 | 0.07 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0.2 | -0.3 | -1.2 | 0.4 | -0.4 | -0.3 | 1.7 | -0.7 | 0.7 | -2.9 | -6.6 | -7.3 | -5.4 | -6.1 | -5.2 |
Bud | 0.1 | -0.7 | 0.3 | -0.2 | 2.1 | 3.0 | 0.1 | 1.6 | 0.9 | -1.7 | 0 | 0 | -2.1 | -1.2 | -2.4 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -2.2 | -3.5 | -7.4 | -5.7 | -7.4 | -4.8 | -5.1 | -4.7 | -5.4 | 2.2 | 1.8 | 3.0 | 1.4 | 0.2 | -0.3 |
Cytoplasm | 4.3 | 3.3 | 6.2 | 4.8 | 5.3 | 5.9 | 4.5 | 3.9 | 4.5 | 2.3 | 1.7 | 2.4 | 2.9 | 3.6 | 5.0 |
Endoplasmic Reticulum | -0.9 | 2.2 | 0.7 | -1.0 | -2.5 | -5.8 | -4.2 | -5.7 | -4.8 | -3.2 | -1.1 | -3.6 | -8.2 | -6.9 | -7.4 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.5 | 1.3 | 5.3 | 5.5 | 7.2 | 8.2 | 8.6 | 10.1 | 10.9 | 0 | 0 | 0 | 2.2 | 1.5 | 1.3 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |