Standard name
Human Ortholog
Description Cyclin (Bur2p)-dependent protein kinase; part of the BUR kinase complex which functions in transcriptional regulation; phosphorylates the carboxy-terminal domain (CTD) of Rpo21p and the C-terminal repeat domain of Spt5p; recruits Spt6p to the CTD at the onset of transcription; regulated by Cak1p; similar to metazoan CDK9 proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.07 0 0 0 0.05 0 0 0 0.08 0.07 0.13 0.06 0.08 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0.12 0.11 0.18 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.96 0.94 0.93 0.97 0.93 0.92 0.91 0.91 0.86 0.86 0.84 0.87 0.88 0.91 0.84 0.88 0.87 0.88 0.95 0.93 0.9 0.96 0.82 0.84
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0.05 0.08 0.07 0.09 0.14 0.13 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0.09 0.1
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 2 1 1 2 3 0 0 0 0 0 0 2 1 1 0 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 1 1 1 2 6 2 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 1 0 0 0 0 0 0
Cytoplasm 4 7 0 3 19 13 15 23 16 13 12 9 8 16 34 16 43 24 0 0 0 0 4 2
Endoplasmic Reticulum 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 1 7 2 0 0 0 0 0 0
Endosome 1 1 1 0 0 1 0 1 0 0 0 1 0 0 1 1 3 6 0 1 0 0 3 1
Golgi 0 1 0 0 0 0 0 1 0 0 0 0 0 0 0 1 3 0 2 0 0 0 0 0
Mitochondria 12 2 1 0 1 10 24 14 38 47 68 53 1 0 1 9 6 8 2 2 0 0 1 2
Nucleus 312 203 63 165 246 284 459 511 270 364 318 346 84 211 215 230 490 362 308 202 72 51 210 293
Nuclear Periphery 6 0 0 0 1 0 3 0 0 3 0 4 0 0 1 0 1 1 0 0 0 0 0 1
Nucleolus 12 1 3 1 0 12 23 43 22 38 52 51 3 2 6 6 6 9 1 0 0 0 6 6
Peroxisomes 0 0 0 0 0 0 1 0 0 1 0 0 0 2 2 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1 0 2 4 1 2 1 1 0 3 3 0 2 0 2 0 0 0 4 2
Vac/Vac Membrane 3 5 1 2 2 2 6 3 4 5 2 1 0 4 2 8 16 19 5 4 2 0 22 33
Unique Cell Count 326 215 68 170 265 309 503 562 313 423 377 397 96 232 255 261 563 413 325 217 81 54 257 351
Labelled Cell Count 350 220 69 171 270 324 535 603 354 481 457 475 96 239 265 272 580 432 325 217 81 54 257 351


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.9 8.5 7.7 8.1 8.4 6.9 6.9 7.0 6.2 6.2 5.8 5.8 8.4 8.9 8.6 10.8 11.2 9.6 6.9 8.3 8.6
Std Deviation (1e-4) 4.8 1.3 1.3 1.3 1.4 1.2 1.5 1.6 1.7 1.5 1.5 1.4 1.9 1.8 1.9 2.4 2.6 2.3 0.7 1.4 1.3
Intensity Change (Log2) – – – 0.08 0.13 -0.14 -0.16 -0.14 -0.31 -0.3 -0.4 -0.41 0.13 0.21 0.17 0.5 0.54 0.32 -0.16 0.11 0.17


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 3.2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 2.6 2.5 3.5 2.8 0 0 0 0 0 0
Nucleus 1.5 0.1 -0.2 -0.4 -0.5 -1.4 -1.5 -1.8 -1.3 -1.1 -0.4 -1.8 -1.1 -1.3 -1.2
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 1.3 2.2 2.0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 4.2139 5.0967 4.1487 4.0772 3.5131 4.0653 5.2153 5.7332 5.1859 4.6317 4.1267 4.908 2.867 3.3561 2.9317 2.5931 2.2866 2.6565
Actin 0.0183 0 0.0082 0.0357 0.0254 0.0026 0.0234 0 0.0005 0.0016 0 0.0054 0.0246 0 0.0004 0 0.0229 0
Bud 0.0013 0 0.0004 0.0009 0.0021 0.0001 0.0005 0 0 0.0001 0 0.0001 0.0002 0 0.0002 0 0.0002 0.0001
Bud Neck 0.0031 0 0.0027 0.0018 0.0028 0.0008 0.0004 0 0.0001 0.0003 0.0002 0.0005 0.0008 0 0.0004 0 0.0008 0.0005
Bud Periphery 0.0023 0 0.0009 0.0018 0.007 0.0001 0.0009 0 0 0.0001 0.0001 0.0001 0.0002 0 0.0004 0 0.0005 0.0002
Bud Site 0.0083 0 0.0074 0.0028 0.0041 0.0001 0.0027 0 0.0002 0.0004 0.0001 0.0001 0.0006 0 0.0006 0 0.0011 0
Cell Periphery 0.0005 0 0.0005 0.0003 0.0013 0 0.0001 0 0 0.0001 0 0 0.0001 0 0.0001 0 0.0002 0
Cytoplasm 0.0061 0.0001 0.0007 0.0003 0.0014 0.0001 0.0042 0.0001 0.0003 0.0017 0.0001 0.0001 0.0042 0 0.0015 0 0.0008 0
Cytoplasmic Foci 0.0164 0.0002 0.0108 0.0017 0.0047 0.0004 0.0212 0 0.002 0.0122 0.0165 0.0001 0.0055 0 0.0001 0 0.0018 0
Eisosomes 0.0002 0 0.0002 0.0013 0.0005 0 0.0002 0 0 0 0 0.0001 0.0003 0 0 0 0.0006 0
Endoplasmic Reticulum 0.0106 0.0001 0.0006 0.0006 0.0025 0.0003 0.002 0 0.0002 0.0011 0 0.0007 0.0068 0 0.0009 0 0.0005 0
Endosome 0.0359 0.0008 0.0104 0.0035 0.0151 0.0034 0.0111 0 0.0146 0.0172 0.0032 0.0013 0.0186 0 0.0004 0 0.0033 0
Golgi 0.0072 0.0001 0.0054 0.0053 0.0091 0.002 0.0028 0 0.0021 0.0058 0.0012 0.0012 0.004 0 0 0 0.0029 0
Lipid Particles 0.0176 0.0008 0.017 0.0068 0.027 0.002 0.0044 0 0.0007 0.0192 0.0192 0.0002 0.0055 0 0 0 0.0102 0
Mitochondria 0.0319 0.0001 0.0103 0.0225 0.0507 0.0071 0.0059 0.0001 0.0005 0.0022 0.0004 0.0011 0.0015 0.0001 0.0008 0.0001 0.0081 0.0008
None 0.0046 0.0001 0.0004 0.0006 0.0011 0.0001 0.0025 0 0.0002 0.0006 0 0 0.005 0 0.0001 0 0.0017 0
Nuclear Periphery 0.0258 0.0027 0.0026 0.0036 0.0353 0.0025 0.0254 0.0005 0.0003 0.0052 0.0012 0.0006 0.0146 0.0003 0.0031 0.0003 0.004 0.0002
Nucleolus 0.0203 0.0068 0.0137 0.0098 0.0661 0.0314 0.0075 0.0043 0.0029 0.0048 0.0554 0.019 0.0087 0.0045 0.0071 0.0042 0.0627 0.0201
Nucleus 0.734 0.9862 0.8954 0.8927 0.6987 0.9445 0.8627 0.9917 0.9739 0.89 0.8947 0.9691 0.8893 0.9949 0.9825 0.9953 0.8685 0.9777
Peroxisomes 0.0172 0 0.0031 0.0036 0.026 0.0002 0.0035 0 0.0004 0.0029 0.0047 0 0.0007 0 0 0 0.0048 0
Punctate Nuclear 0.0209 0.0016 0.0019 0.0015 0.0055 0.0017 0.0074 0.0034 0.0001 0.0115 0.0025 0.0001 0.0069 0 0.0004 0 0.0026 0.0001
Vacuole 0.0126 0.0002 0.0043 0.0014 0.01 0.0002 0.0034 0 0.0006 0.0111 0.0002 0.0001 0.0012 0.0001 0.0008 0 0.0008 0.0001
Vacuole Periphery 0.0049 0.0001 0.0034 0.0014 0.0038 0.0002 0.0076 0 0.0003 0.0119 0.0001 0.0001 0.0009 0 0.0002 0 0.0008 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 27.669 26.4833 21.1349 36.4586 21.3795 15.0838 27.1561 27.7912 27.0556 31.6195
Translational Efficiency 0.7707 0.9972 1.1885 0.7213 1.0446 1.2477 1.0106 0.8168 0.917 0.76

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
2073 1450 2263 1235 1386 1097 62 258 3459 2547 2325 1493

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 725.09 861.70 1004.12 1106.65 772.24 811.55 1201.20 1231.54 743.98 840.10 1009.38 1128.23
Standard Deviation 104.56 130.23 138.55 177.24 111.70 132.93 142.95 209.89 109.93 133.72 142.26 189.29
Intensity Change Log 2 — 0.249026 0.469700 0.609967 — 0.071631 0.637355 0.673342 — 0.160261 0.558596 0.643000

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000192 0.000440 0.000631 0.001482 0.000350 0.000495 0.001410 0.001004 0.000255 0.000464 0.000652 0.001399
Bud Neck 0.003424 0.006554 0.019406 0.036077 0.003208 0.004812 0.004473 0.028418 0.003337 0.005804 0.019008 0.034753
Bud Site 0.000878 0.000584 0.001951 0.017477 0.000443 0.001292 0.001090 0.003716 0.000703 0.000889 0.001928 0.015099
Cell Periphery 0.000285 0.000090 0.000110 0.000317 0.000124 0.000188 0.000049 0.000186 0.000221 0.000132 0.000108 0.000294
Cytoplasm 0.004478 0.001524 0.000746 0.006465 0.002941 0.002182 0.000696 0.001977 0.003862 0.001807 0.000745 0.005690
Cytoplasmic Foci 0.000286 0.000388 0.000026 0.005633 0.000565 0.002855 0.000607 0.002109 0.000398 0.001450 0.000042 0.005024
Eisosomes 0.000023 0.000021 0.000043 0.000041 0.000020 0.000032 0.000067 0.000042 0.000022 0.000026 0.000044 0.000041
Endoplasmic Reticulum 0.004139 0.004031 0.003675 0.003269 0.004272 0.005291 0.005931 0.004732 0.004192 0.004574 0.003736 0.003521
Endosome 0.000278 0.000415 0.000162 0.007423 0.000214 0.003493 0.000542 0.003597 0.000252 0.001740 0.000172 0.006762
Golgi 0.000064 0.000203 0.000044 0.006878 0.000629 0.002047 0.000231 0.004630 0.000290 0.000997 0.000049 0.006490
Lipid Particles 0.000478 0.000590 0.000051 0.000692 0.000744 0.001509 0.000273 0.000202 0.000584 0.000986 0.000057 0.000607
Mitochondria 0.001242 0.000831 0.001599 0.007039 0.001172 0.002238 0.001035 0.002891 0.001213 0.001437 0.001584 0.006322
Mitotic Spindle 0.000672 0.000280 0.002094 0.035668 0.001253 0.002623 0.000046 0.016827 0.000905 0.001289 0.002039 0.032412
None 0.010312 0.003789 0.001373 0.005758 0.006126 0.004959 0.000405 0.003310 0.008635 0.004293 0.001347 0.005335
Nuclear Periphery 0.000262 0.000241 0.000353 0.005430 0.000194 0.000614 0.000137 0.000777 0.000235 0.000401 0.000347 0.004626
Nuclear Periphery Foci 0.000269 0.000766 0.000090 0.002699 0.000216 0.001512 0.001624 0.000188 0.000248 0.001088 0.000131 0.002265
Nucleolus 0.009216 0.009991 0.003888 0.007437 0.008815 0.010861 0.007802 0.008813 0.009055 0.010366 0.003993 0.007674
Nucleus 0.962240* 0.968120* 0.962775* 0.836071* 0.967934* 0.946707* 0.961723* 0.911552* 0.964522* 0.958897* 0.962747* 0.849114*
Peroxisomes 0.000276 0.000263 0.000163 0.000674 0.000370 0.001397 0.000965 0.000352 0.000314 0.000752 0.000184 0.000619
Vacuole 0.000760 0.000507 0.000722 0.009020 0.000321 0.003014 0.010601 0.002740 0.000584 0.001587 0.000986 0.007935
Vacuole Periphery 0.000227 0.000373 0.000096 0.004451 0.000092 0.001881 0.000292 0.001936 0.000173 0.001022 0.000101 0.004016

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -6.13 -15.63 -6.92 -5.52 -4.27 -1.09 -6.51 -4.70 -4.49 0.39 -3.84 -8.92 -7.40 -6.28 -4.78
Bud Neck -4.60 -19.73 -15.22 -13.25 -6.07 -2.50 -1.72 -6.60 -6.25 -6.23 -5.19 -20.08 -16.62 -15.07 -6.73
Bud Site 0.94 -2.86 -6.29 -6.53 -5.51 -2.69 -1.80 -4.51 -3.65 -3.66 -0.86 -3.73 -6.95 -6.83 -5.84
Cell Periphery 4.68 4.62 1.40 -3.59 -3.49 -1.29 3.07 -1.09 0.24 -2.88 2.72 4.44 0.31 -2.28 -3.77
Cytoplasm 3.23 4.48 -0.77 -3.65 -4.65 0.68 2.56 1.00 0.23 -1.85 3.02 5.51 -1.03 -3.32 -4.91
Cytoplasmic Foci -0.48 2.78 -3.24 -2.91 -3.61 -2.80 -0.05 -0.65 1.69 -0.48 -2.75 3.32 -3.17 -1.46 -3.81
Eisosomes 1.00 -8.85 -7.52 -10.66 0.86 -4.49 -8.37 -6.99 -3.94 2.60 -2.34 -12.04 -10.79 -8.93 0.40
Endoplasmic Reticulum 0.36 1.00 1.51 1.19 0.43 -2.43 -2.90 -0.01 0.76 1.51 -1.62 1.10 1.23 2.35 0.27
Endosome -0.68 1.53 -4.71 -4.42 -4.87 -3.63 -0.79 -1.44 1.05 -1.00 -3.66 1.46 -4.95 -2.39 -5.07
Golgi -1.71 0.81 -3.67 -3.50 -3.68 -1.78 0.73 -0.81 -0.16 -1.01 -2.10 1.23 -3.46 -2.60 -3.80
Lipid Particles -0.33 1.36 0.09 0.50 -2.42 -0.83 0.74 0.91 1.75 0.38 -0.91 1.84 0.55 1.56 -2.61
Mitochondria 0.78 -0.86 -4.08 -4.97 -4.01 -1.63 0.20 -3.34 -1.73 -4.40 -0.55 -1.17 -4.69 -4.63 -4.35
Mitotic Spindle 0.84 -1.71 -7.74 -7.90 -7.32 -1.16 2.11 -2.69 -2.48 -2.85 -0.68 -1.48 -8.15 -8.00 -7.72
None 4.42 7.26 3.80 -0.62 -3.66 0.71 5.47 2.28 1.23 -3.77 3.91 8.56 3.84 0.01 -4.07
Nuclear Periphery 0.33 -2.33 -3.84 -3.87 -3.68 -1.43 0.52 -4.54 -1.60 -4.60 -1.26 -3.43 -4.09 -3.82 -3.87
Nuclear Periphery Foci -1.17 1.93 -3.09 -1.36 -3.60 -2.29 -0.90 -0.10 2.27 0.89 -2.43 1.54 -3.12 -0.53 -3.51
Nucleolus -0.85 7.27 -0.48 0.12 -4.25 -1.60 0.61 -0.49 0.32 -0.79 -1.73 8.36 -0.70 0.44 -4.68
Nucleus -1.70 1.11 12.64 13.43 12.52 3.77 0.70 5.29 3.49 2.98 1.89 2.19 13.99 12.68 13.22
Peroxisomes -0.03 0.99 -1.37 -1.60 -2.87 -2.37 -2.21 -0.16 2.42 2.42 -2.22 1.42 -1.21 1.42 -3.02
Vacuole 0.81 -0.12 -6.73 -7.17 -6.93 -3.11 -1.45 -3.87 -1.27 0.94 -2.40 -1.76 -7.74 -5.93 -6.83
Vacuole Periphery -0.74 2.12 -3.92 -3.50 -4.16 -2.66 -0.99 -1.79 0.87 -1.26 -2.73 1.82 -4.27 -2.14 -4.42
Standard name
Human Ortholog
Description Cyclin (Bur2p)-dependent protein kinase; part of the BUR kinase complex which functions in transcriptional regulation; phosphorylates the carboxy-terminal domain (CTD) of Rpo21p and the C-terminal repeat domain of Spt5p; recruits Spt6p to the CTD at the onset of transcription; regulated by Cak1p; similar to metazoan CDK9 proteins
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-4

Sgv1

Sgv1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sgv1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available