Standard name
Human Ortholog
Description Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; contains a HORMA domain required for autophagy and for recruitment of the phosphatidylinositol 3-kinase complex subunit Atg14p to the pre-autophagosomal structure
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.88 0.8 0.82 0.84 0.83 0.71 – 0.69 0.78 0.97 0.95 0.94 0.93 0.96 0.93 0.94 0.93 0.88 0.77 0.67 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Endosome 0 0 0 0 0.06 0.08 0.07 0.05 0 – 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.07 0 0.28 – 0.33 0.15 0 0 0 0 0 0 0 0 0 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0.11 0 0.05 0.09 0 – 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0.05 0 0.06 0 0 0.05 0.1 0.15 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 9 12 3 3 10 4 – 2 6 0 1 1 2 0 0 0 0 0 0 1 2
Bud 0 0 4 2 7 9 13 13 9 – 10 8 3 1 6 0 0 0 0 0 0 2 2 4
Bud Neck 0 0 1 3 2 1 4 5 2 – 2 2 0 0 0 0 0 0 1 0 0 0 1 4
Bud Site 0 0 0 0 0 0 1 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 2 0 1 0 3 5 1 – 4 3 3 1 3 12 9 13 0 0 0 0 0 0
Cytoplasm 167 70 258 196 263 253 382 456 229 – 194 252 387 396 476 461 247 243 161 81 238 132 244 281
Endoplasmic Reticulum 2 0 2 4 4 5 2 1 11 – 8 5 6 4 6 18 2 10 0 0 2 7 18 11
Endosome 0 0 0 3 20 26 30 27 13 – 14 17 7 18 20 13 1 4 2 0 1 2 11 11
Golgi 1 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 2 2 0 0 0 0 0 0
Mitochondria 4 3 1 1 1 14 33 24 91 – 94 47 1 2 1 5 0 0 2 2 7 5 18 13
Nucleus 0 0 0 2 1 1 1 5 4 – 0 2 0 0 2 0 3 1 0 0 0 0 0 1
Nuclear Periphery 1 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 – 0 1 0 0 0 1 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 1 0 0 2 – 0 2 0 2 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 14 35 13 21 48 11 – 6 27 1 2 3 2 0 0 0 0 0 0 2 1
Vac/Vac Membrane 0 0 1 0 11 7 2 9 6 – 8 2 0 4 4 27 10 15 0 2 13 17 54 69
Unique Cell Count 170 71 261 223 329 308 456 552 322 283 322 400 419 506 497 258 262 171 88 270 173 362 410
Labelled Cell Count 176 73 269 234 357 333 495 603 383 343 374 408 431 524 543 274 288 171 88 270 173 362 410


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.2 5.6 5.3 5.9 4.7 4.8 5.1 4.2 – 4.0 4.6 5.5 5.8 5.9 10.6 10.6 10.3 5.3 5.2 5.6
Std Deviation (1e-4) 0.6 0.7 0.7 0.9 1.2 1.1 1.0 0.9 0.8 – 0.6 0.7 0.7 0.9 1.0 1.4 1.3 1.4 1.1 1.0 1.0
Intensity Change (Log2) – – – -0.07 0.07 -0.25 -0.21 -0.14 -0.4 – -0.5 -0.29 -0.02 0.04 0.07 0.91 0.92 0.88 -0.09 -0.11 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 3.1 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.8 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 2.2 2.9
Cytoplasm -5.0 -7.1 -6.6 -6.3 -6.6 -9.0 – -9.4 -7.5 -1.7 -2.9 -3.1 -3.6 -2.2 -3.5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 2.3 0 2.3
Endosome 0 4.1 0 0 3.6 0 – 0 0 0 3.4 3.3 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.1 0 – 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 4.1 5.4 0 0 4.9 0 – 0 0 0 0 0 0 0 0
Vacuole 0 2.5 0 0 0 0 – 0 0 0 0 0 3.5 2.8 3.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.703 0.0406 -0.2896 -0.5036 -0.8933 -0.124 -0.099 0.6595 0.3798 0.343 -0.2211 0.1709 0.0077 0.8378 0.4584 0.6046 0.5644 0.3797
Actin 0.0235 0.0083 0.0073 0.0405 0.0074 0.0036 0.0301 0.0003 0.006 0.0517 0.0027 0.0018 0.0458 0.0002 0.0263 0.0182 0.0079 0.002
Bud 0.001 0.0012 0.0011 0.0007 0.0005 0.0007 0.0046 0.0006 0.0006 0.0012 0.0004 0.0004 0.0024 0.0026 0.0007 0.0006 0.0004 0.0001
Bud Neck 0.0096 0.0002 0.0032 0.0091 0.0029 0.0012 0.017 0.0002 0.0003 0.0026 0.0004 0.0005 0.0015 0.0005 0.0007 0.0012 0.0008 0.0005
Bud Periphery 0.0018 0.002 0.0012 0.001 0.0007 0.0008 0.0066 0.0002 0.0004 0.0017 0.0002 0.0009 0.0021 0.0009 0.0009 0.0005 0.0003 0.0003
Bud Site 0.0113 0.0012 0.0075 0.0021 0.0018 0.0002 0.0185 0.0031 0.0032 0.0045 0.0003 0.0002 0.0025 0.0192 0.0027 0.0071 0.0032 0.0002
Cell Periphery 0.001 0.0003 0.0005 0.0003 0.0003 0.0002 0.0007 0.0001 0.0002 0.0003 0.0001 0.0001 0.0003 0.0003 0.0002 0.0002 0.0001 0.0002
Cytoplasm 0.3379 0.5628 0.4755 0.3862 0.4885 0.6735 0.5609 0.8445 0.7673 0.6901 0.591 0.8078 0.5811 0.7999 0.6931 0.6707 0.6589 0.8031
Cytoplasmic Foci 0.0401 0.023 0.0358 0.0556 0.0814 0.0177 0.0581 0.014 0.0245 0.0419 0.0343 0.0409 0.0408 0.0145 0.0264 0.0443 0.0464 0.0192
Eisosomes 0.0008 0.0002 0.0002 0.0009 0.0001 0.0002 0.0002 0 0.0001 0.0008 0 0.0001 0.0003 0 0.0004 0.0001 0.0002 0
Endoplasmic Reticulum 0.0054 0.0033 0.0065 0.0065 0.0028 0.0023 0.005 0.0028 0.0041 0.0019 0.0015 0.0031 0.0111 0.0026 0.0034 0.0025 0.0043 0.0037
Endosome 0.0527 0.0269 0.0362 0.0737 0.116 0.017 0.0441 0.0105 0.0367 0.0828 0.134 0.0201 0.0529 0.0234 0.0305 0.0968 0.0388 0.0365
Golgi 0.0051 0.0016 0.0057 0.02 0.0139 0.0015 0.01 0.0005 0.0057 0.0136 0.0726 0.0012 0.0104 0.0005 0.0046 0.0052 0.0043 0.0021
Lipid Particles 0.0044 0.0012 0.0079 0.0385 0.0127 0.0022 0.0115 0.0004 0.0076 0.0018 0.0025 0.0005 0.0069 0.0002 0.0052 0.0051 0.0094 0.0033
Mitochondria 0.013 0.001 0.0116 0.0718 0.0234 0.0209 0.0123 0.0001 0.003 0.0267 0.0114 0.0183 0.0115 0.0002 0.0067 0.002 0.0014 0.0097
None 0.4605 0.3539 0.3679 0.2593 0.2187 0.2422 0.1604 0.1151 0.124 0.0556 0.13 0.0936 0.1891 0.1283 0.1737 0.1246 0.1523 0.1119
Nuclear Periphery 0.0069 0.0009 0.0023 0.0023 0.001 0.0054 0.0098 0.0006 0.0011 0.0009 0.0007 0.0019 0.0069 0.0006 0.0019 0.0011 0.0046 0.0006
Nucleolus 0.0011 0.0002 0.0032 0.0003 0.0012 0.0003 0.0019 0.0001 0.0005 0.0001 0.0001 0.0001 0.0004 0.0001 0.0005 0.0001 0.0013 0.0001
Nucleus 0.0065 0.0011 0.0026 0.0013 0.001 0.0011 0.0029 0.0007 0.0011 0.0013 0.0007 0.0008 0.0017 0.0008 0.0012 0.001 0.0061 0.0007
Peroxisomes 0.0037 0.0003 0.0083 0.0152 0.0091 0.0006 0.0209 0.0003 0.0028 0.0043 0.0014 0.0026 0.0148 0.0001 0.009 0.0042 0.0447 0.0009
Punctate Nuclear 0.0051 0.0004 0.002 0.002 0.0018 0.0026 0.0078 0.0003 0.0007 0.0003 0.0002 0.0009 0.0017 0.0002 0.0029 0.0007 0.0083 0.0004
Vacuole 0.0075 0.0088 0.0119 0.0098 0.0114 0.0046 0.0137 0.0051 0.0087 0.013 0.012 0.0032 0.0134 0.0044 0.0077 0.0116 0.0051 0.0037
Vacuole Periphery 0.0012 0.001 0.0016 0.0029 0.0033 0.0013 0.003 0.0006 0.0013 0.003 0.0034 0.0011 0.0024 0.0006 0.0014 0.0022 0.001 0.0008

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 9.7052 4.8941 7.5934 11.1562 7.7435 11.4408 10.0092 12.6002 13.2337 14.5532
Translational Efficiency 1.1168 1.0864 0.6003 0.519 0.9365 1.0676 0.7071 0.4582 0.6259 0.5892

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✔
Standard name
Human Ortholog
Description Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; contains a HORMA domain required for autophagy and for recruitment of the phosphatidylinositol 3-kinase complex subunit Atg14p to the pre-autophagosomal structure
Localization
Cell Percentages cytoplasm (92%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Atg13

Atg13


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available