Standard name
Description Regulatory subunit of the Atg1p signaling complex; stimulates Atg1p kinase activity; required for vesicle formation during autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; contains a HORMA domain required for autophagy and for recruitment of the phosphatidylinositol 3-kinase complex subunit Atg14p to the pre-autophagosomal structure

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.98 0.99 0.99 0.88 0.8 0.82 0.84 0.83 0.71 0.69 0.78 0.97 0.95 0.94 0.93 0.96 0.93 0.94 0.93 0.88 0.77 0.67 0.69
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
Endosome 0 0 0 0 0.06 0.08 0.07 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0.07 0 0.28 0.33 0.15 0 0 0 0 0 0 0 0 0 0 0.05 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.06 0.11 0 0.05 0.09 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.06 0 0 0.05 0.1 0.15 0.17
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 9 12 3 3 10 4 2 6 0 1 1 2 0 0 0 0 0 0 1 2
Bud 0 0 4 2 7 9 13 13 9 10 8 3 1 6 0 0 0 0 0 0 2 2 4
Bud Neck 0 0 1 3 2 1 4 5 2 2 2 0 0 0 0 0 0 1 0 0 0 1 4
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2 0 1 0 3 5 1 4 3 3 1 3 12 9 13 0 0 0 0 0 0
Cytoplasm 167 70 258 196 263 253 382 456 229 194 252 387 396 476 461 247 243 161 81 238 132 244 281
Endoplasmic Reticulum 2 0 2 4 4 5 2 1 11 8 5 6 4 6 18 2 10 0 0 2 7 18 11
Endosome 0 0 0 3 20 26 30 27 13 14 17 7 18 20 13 1 4 2 0 1 2 11 11
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 2 2 2 0 0 0 0 0 0
Mitochondria 4 3 1 1 1 14 33 24 91 94 47 1 2 1 5 0 0 2 2 7 5 18 13
Nucleus 0 0 0 2 1 1 1 5 4 0 2 0 0 2 0 3 1 0 0 0 0 0 1
Nuclear Periphery 1 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 1 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 0 0 0 0 0 0 0 1
Peroxisomes 0 0 0 0 0 1 0 0 2 0 2 0 2 2 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 14 35 13 21 48 11 6 27 1 2 3 2 0 0 0 0 0 0 2 1
Vac/Vac Membrane 0 0 1 0 11 7 2 9 6 8 2 0 4 4 27 10 15 0 2 13 17 54 69
Unique Cell Count 170 71 261 223 329 308 456 552 322 283 322 400 419 506 497 258 262 171 88 270 173 362 410
Labelled Cell Count 176 73 269 234 357 333 495 603 383 343 374 408 431 524 543 274 288 171 88 270 173 362 410


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.2 5.6 5.3 5.9 4.7 4.8 5.1 4.2 4.0 4.6 5.5 5.8 5.9 10.6 10.6 10.3 5.3 5.2 5.6
Std Deviation (1e-4) 0.6 0.7 0.7 0.9 1.2 1.1 1.0 0.9 0.8 0.6 0.7 0.7 0.9 1.0 1.4 1.3 1.4 1.1 1.0 1.0
Intensity Change (Log2) -0.07 0.07 -0.25 -0.21 -0.14 -0.4 -0.5 -0.29 -0.02 0.04 0.07 0.91 0.92 0.88 -0.09 -0.11 -0.0


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 3.1 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0.8 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 2.2 2.9
Cytoplasm -5.0 -7.1 -6.6 -6.3 -6.6 -9.0 -9.4 -7.5 -1.7 -2.9 -3.1 -3.6 -2.2 -3.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 2.3 0 2.3
Endosome 0 4.1 0 0 3.6 0 0 0 0 3.4 3.3 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 3.1 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 4.1 5.4 0 0 4.9 0 0 0 0 0 0 0 0 0
Vacuole 0 2.5 0 0 0 0 0 0 0 0 0 3.5 2.8 3.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Atg13-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available