Standard name
Human Ortholog
Description Subunit 2 of ubiquinol cytochrome-c reductase (Complex III); Complex III is a component of the mitochondrial inner membrane electron transport chain; phosphorylated; transcription is regulated by Hap1p, Hap2p/Hap3p, and heme

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0.07 0 0.06 0.11 0.1
Bud 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0.05 0.11 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.11 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0.06 0.09 0.08 0.06 0 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0.2 0.09 0.31 0.17 0.16 0.17 0.08 0.13 0.06 0.1 0.12 0.13
Mitochondria 0.9 0.92 0.96 0.94 0.85 0.89 0.88 0.83 0.89 0.95 0.92 0.87 0.85 0.74 0.08 0.07 0.11 0.69 0.67 0.69 0.57 0.54 0.53
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.07 0.05 0.05 0 0.05 0.07 0.09 0 0.06 0.06 0 0 0 0 0 0.07 0 0 0 0 0 0 0
Nucleolus 0.05 0.05 0 0 0.06 0.1 0.17 0.19 0.11 0.12 0.11 0 0 0 0 0.08 0.06 0 0 0 0 0 0
Peroxisomes 0.05 0 0.11 0.1 0.06 0 0 0 0 0 0 0.06 0.14 0 0.36 0.33 0.38 0.11 0.07 0.18 0.19 0.14 0.09
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.08 0 0 0.07 0 0 0 0 0 0 0.12 0.09 0.15 0.12 0.12 0.13 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 0 3 2 4 0 0 1 7 2 1 8 3 3 6 9 4 18 35 32
Bud 1 0 9 13 20 26 18 20 13 18 9 4 7 2 5 4 10 0 0 0 1 1 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 4 0 0 8 3 7 10 5 3 0 0 0 1 0 0
Cell Periphery 2 6 4 5 6 2 5 3 7 3 1 6 9 6 10 2 3 0 0 0 0 0 0
Cytoplasm 1 1 0 8 15 14 12 14 10 6 6 1 2 5 7 8 7 0 0 0 1 0 4
Endoplasmic Reticulum 4 3 3 1 1 4 1 3 1 1 1 1 0 0 3 4 2 0 0 0 2 2 3
Endosome 5 0 10 2 27 5 5 5 2 2 2 4 19 14 11 6 6 11 4 12 9 10 15
Golgi 7 1 3 0 2 0 0 1 0 1 4 40 32 71 20 12 16 21 19 21 28 40 42
Mitochondria 219 135 346 217 424 598 869 782 741 673 459 176 300 167 10 5 10 177 101 240 166 178 175
Nucleus 2 0 0 0 0 4 4 4 4 0 2 0 1 2 2 1 0 0 0 0 0 0 0
Nuclear Periphery 18 8 17 4 26 50 87 42 52 39 19 4 5 3 4 5 1 3 0 1 1 5 4
Nucleolus 11 8 14 4 31 64 164 180 91 86 55 2 7 3 4 6 6 0 0 0 0 1 3
Peroxisomes 12 1 40 22 31 16 13 29 25 6 4 13 48 9 44 25 36 29 10 63 55 46 30
SpindlePole 1 1 7 0 9 5 5 7 12 6 3 1 6 0 7 3 2 1 0 1 2 0 1
Vac/Vac Membrane 18 12 8 0 35 17 23 21 28 16 21 24 32 34 14 9 12 6 2 0 3 3 8
Unique Cell Count 244 146 359 231 500 670 993 940 834 709 499 203 351 227 121 76 94 259 153 350 295 329 333
Labelled Cell Count 301 176 467 276 627 816 1211 1122 996 862 590 283 470 317 150 93 114 259 153 350 295 329 333


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.4 8.9 6.6 7.1 8.0 9.3 9.8 11.5 13.0 14.6 15.3 10.6 10.6 10.4 10.2 9.7 10.5 13.6 15.8 17.3
Std Deviation (1e-4) 3.0 2.1 1.7 1.8 1.9 2.1 2.7 3.4 5.2 5.9 6.8 2.2 4.0 2.7 3.6 2.9 4.1 9.7 13.6 13.9
Intensity Change (Log2) 0.1 0.28 0.49 0.56 0.8 0.97 1.14 1.21 0.68 0.68 0.65 0.62 0.55 0.67 1.03 1.25 1.39


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 2.0 1.2 1.2 -0.8 -0.4 -1.1 0 -0.7 0 -0.5 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 1.4 0 0 0 0
Cytoplasm 0 3.3 0 0 0 0 0 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 1.9 -2.6 0 0 0 0 -2.9 -0.6 1.8 2.0 2.9 0 0
Golgi 0 0 0 0 0 0 0 0 8.1 5.1 10.8 7.0 0 0
Mitochondria -1.4 -5.5 -4.0 -4.8 -6.3 -4.2 -1.1 -2.6 -4.3 -5.1 -8.1 -19.2 -18.0 -18.0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery -1.9 0.3 1.7 2.5 -0.2 1.0 0.5 -0.7 -1.7 -2.5 -2.2 -0.7 0 0
Nucleolus -1.5 1.5 3.3 6.1 6.9 3.9 4.4 3.8 -2.0 -1.5 -1.8 0 0 0
Peroxisomes -0.6 -2.6 -5.9 -8.2 -5.8 -5.7 -7.8 -6.8 -1.8 1.0 -3.1 6.3 4.8 6.3
SpindlePole 0 -0.2 0 0 0 -0.6 0 0 0 -0.2 0 0 0 0
Vacuole 0 3.2 0.3 0.1 0 1.0 0 1.6 4.7 4.0 5.8 4.2 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.0633 21.3716 19.4856 18.6228 18.1012 20.0735 22.6143 26.2378 24.1486 24.7 20.3343 24.51 24.5017 25.5831 24.6167 25.2521 24.9459 26.024
Actin 0.0241 0.0221 0.0129 0.004 0.0047 0.0078 0.0147 0.0023 0.0269 0.0354 0.0037 0.0045 0.0123 0.0086 0.0041 0.0136 0.0081 0.0009
Bud 0.0001 0.0001 0 0 0 0 0.0004 0 0.0001 0.0006 0 0.0001 0 0.0001 0 0.0001 0 0
Bud Neck 0.0001 0.0001 0 0 0 0 0.0005 0 0 0 0 0.0005 0.0001 0 0 0.0009 0 0
Bud Periphery 0.0003 0.0002 0 0 0.0001 0 0.0008 0 0.0001 0.0007 0.0001 0.0001 0 0.0001 0 0.0005 0 0
Bud Site 0.0004 0.0003 0 0.0001 0.0001 0 0.0019 0 0.0004 0.0012 0.0001 0.0004 0.0001 0.0001 0.0001 0.0001 0 0
Cell Periphery 0.0002 0.0002 0.0001 0.0001 0.0001 0.0001 0.0003 0.0001 0 0.0001 0 0.0001 0.0001 0.0002 0.0001 0.0001 0 0
Cytoplasm 0 0 0 0 0 0 0.0006 0 0.0001 0 0 0 0.0001 0 0 0.0003 0 0
Cytoplasmic Foci 0.0041 0.0023 0.0007 0.0016 0.0028 0.0007 0.0044 0.0006 0.0024 0.0003 0.0005 0.0013 0.0008 0.0007 0.0024 0.0002 0.0001 0.0002
Eisosomes 0.0016 0.0017 0.0009 0.0005 0.0005 0.001 0.0011 0.0003 0.0012 0.0004 0.0005 0.0005 0.0008 0.0004 0.0007 0.0012 0.0005 0.0003
Endoplasmic Reticulum 0.0001 0 0 0 0.0001 0 0.0003 0 0.0002 0 0 0 0.0001 0 0 0.0023 0 0
Endosome 0.0038 0.0036 0.0017 0.0021 0.0085 0.0019 0.0037 0.0009 0.0074 0.001 0.0007 0.0013 0.0008 0.0015 0.0008 0.0033 0.0005 0.0003
Golgi 0.0505 0.0336 0.0156 0.0084 0.0314 0.0233 0.0044 0.0073 0.0074 0.0359 0.0036 0.0158 0.0048 0.0228 0.0087 0.0043 0.0064 0.0015
Lipid Particles 0.0352 0.0261 0.0117 0.0119 0.0417 0.0096 0.034 0.0078 0.0164 0.0012 0.0238 0.0168 0.0213 0.0119 0.0122 0.0022 0.004 0.0055
Mitochondria 0.8323 0.8849 0.9367 0.9559 0.8971 0.9416 0.8757 0.9696 0.9138 0.9185 0.9551 0.944 0.9397 0.9349 0.9564 0.945 0.9642 0.9853
None 0.0001 0 0 0 0 0 0.016 0 0.0002 0 0 0 0.0002 0 0.0001 0.0011 0 0
Nuclear Periphery 0.0005 0.0001 0 0.0001 0.0001 0 0.0009 0 0.0011 0 0 0.0001 0.0001 0.0001 0 0.0071 0 0.0001
Nucleolus 0.0005 0.0002 0.0001 0.0002 0.0001 0 0.0009 0.0001 0.0001 0 0.0001 0.0001 0.0002 0.0003 0.0004 0.0003 0 0
Nucleus 0.0002 0 0 0 0 0 0.0003 0 0.0001 0 0 0 0.0001 0.0002 0.0001 0.0016 0 0
Peroxisomes 0.0383 0.0162 0.0135 0.0106 0.0099 0.0105 0.0254 0.0086 0.0195 0.0038 0.0112 0.0102 0.0097 0.0071 0.0105 0.0012 0.0032 0.0029
Punctate Nuclear 0 0 0 0 0 0 0.0004 0 0.0007 0 0 0 0.0001 0 0.0002 0.0005 0 0
Vacuole 0.0031 0.0009 0.0004 0.0003 0.0005 0.0003 0.002 0.0003 0.0005 0.0001 0.0001 0.0003 0.0006 0.0022 0.0003 0.0017 0.0002 0.0002
Vacuole Periphery 0.0045 0.0072 0.0055 0.004 0.0022 0.0029 0.0114 0.0021 0.0011 0.0007 0.0005 0.0038 0.008 0.0087 0.0028 0.0125 0.0126 0.0027

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 53.3262 51.6801 60.168 80.9974 62.6781 53.7948 48.8752 54.7257 64.2744 55.059
Translational Efficiency 1.291 1.2675 1.5706 1.5024 1.6627 1.5227 1.536 1.6913 1.648 1.5076

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1237 2032 1121 1034 1551 1482 1096 1752 2788 3514 2217 2786

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 823.51 1137.33 1611.50 2010.07 922.53 1092.97 1513.83 2090.07 878.60 1118.62 1563.22 2060.38
Standard Deviation 149.89 228.71 333.93 554.90 163.55 242.15 295.49 577.97 165.13 235.49 319.27 570.83
Intensity Change Log 2 0.465793 0.968546 1.287388 0.244586 0.714535 1.179884 0.353151 0.839922 1.231587

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002111 0.010540 0.005433 0.009406 0.016201 0.011801 0.002770 0.004831 0.009949 0.011072 0.004116 0.006529
Bud Neck 0.002228 0.000933 0.002136 0.001811 0.001798 0.001578 0.001520 0.000958 0.001989 0.001205 0.001831 0.001274
Bud Site 0.002796 0.002471 0.001328 0.003036 0.001855 0.001751 0.001532 0.001337 0.002273 0.002167 0.001429 0.001968
Cell Periphery 0.000326 0.000155 0.000088 0.000356 0.000283 0.000145 0.000052 0.000283 0.000302 0.000151 0.000070 0.000310
Cytoplasm 0.000506 0.001098 0.000260 0.000375 0.000581 0.000585 0.000302 0.000341 0.000548 0.000881 0.000281 0.000353
Cytoplasmic Foci 0.011755 0.026221 0.029563 0.010849 0.014110 0.021588 0.013191 0.006274 0.013065 0.024267 0.021469 0.007972
Eisosomes 0.000062 0.000537 0.000640 0.000862 0.000593 0.000830 0.000234 0.000707 0.000357 0.000660 0.000439 0.000765
Endoplasmic Reticulum 0.000214 0.000179 0.000255 0.000611 0.000231 0.000169 0.000074 0.000119 0.000223 0.000175 0.000165 0.000302
Endosome 0.022818 0.013112 0.004306 0.003490 0.015714 0.008586 0.003778 0.001706 0.018866 0.011203 0.004045 0.002368
Golgi 0.021415 0.071007 0.045327 0.036165 0.038357 0.049853 0.021208 0.019706 0.030840 0.062086 0.033403 0.025815
Lipid Particles 0.005689 0.016991 0.008847 0.005501 0.017919 0.014359 0.002717 0.001663 0.012493 0.015881 0.005817 0.003087
Mitochondria 0.897029* 0.822396* 0.858566* 0.889309* 0.836532* 0.841628* 0.926352* 0.941446* 0.863374* 0.830507* 0.892077* 0.922096*
Mitotic Spindle 0.001044 0.001849 0.002342 0.004939 0.000626 0.001774 0.003960 0.000819 0.000811 0.001817 0.003142 0.002348
None 0.000273 0.000688 0.004232 0.003097 0.000543 0.000829 0.001036 0.001960 0.000423 0.000748 0.002652 0.002382
Nuclear Periphery 0.000072 0.000149 0.001222 0.000609 0.000155 0.000148 0.000587 0.000168 0.000119 0.000149 0.000908 0.000331
Nuclear Periphery Foci 0.000143 0.000162 0.000585 0.000224 0.000215 0.000202 0.000309 0.000092 0.000183 0.000179 0.000448 0.000141
Nucleolus 0.000169 0.000162 0.000651 0.000459 0.000176 0.000560 0.000258 0.000317 0.000173 0.000330 0.000456 0.000370
Nucleus 0.000134 0.000075 0.000083 0.000085 0.000083 0.000190 0.000069 0.000065 0.000106 0.000124 0.000076 0.000073
Peroxisomes 0.025481 0.027318 0.009926 0.005866 0.051596 0.037425 0.009995 0.005779 0.040009 0.031580 0.009960 0.005812
Vacuole 0.002122 0.001747 0.006376 0.006762 0.001241 0.003606 0.004129 0.004274 0.001632 0.002531 0.005265 0.005197
Vacuole Periphery 0.003615 0.002211 0.017835 0.016188 0.001190 0.002394 0.005927 0.007154 0.002266 0.002288 0.011948 0.010507

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -8.85 -3.47 -4.60 0.61 -2.20 2.09 6.99 5.74 5.06 -1.94 -0.91 5.00 2.65 4.31 -2.51
Bud Neck 1.55 0.09 0.46 -3.42 0.83 0.52 0.62 2.02 1.89 1.69 1.82 0.32 1.57 -0.38 2.16
Bud Site 0.37 1.94 -0.25 -0.59 -1.88 0.17 0.52 0.92 0.74 0.37 0.19 1.80 0.53 0.37 -1.14
Cell Periphery 3.13 5.18 -0.26 -1.64 -2.24 3.03 5.67 -0.02 -1.74 -3.00 4.19 7.60 -0.14 -2.34 -3.65
Cytoplasm -1.85 2.02 0.92 2.35 -1.62 -0.03 1.93 1.48 2.06 -0.38 -1.69 2.78 1.74 2.83 -1.12
Cytoplasmic Foci -8.19 -6.76 0.58 8.78 7.14 -4.55 0.52 5.92 9.80 4.19 -9.19 -5.29 5.05 13.84 8.67
Eisosomes -13.35 -10.47 -7.49 -2.89 -1.85 -2.63 6.06 -0.95 0.96 -4.39 -6.31 -1.88 -4.86 -1.22 -3.92
Endoplasmic Reticulum 0.98 -0.90 -1.66 -1.80 -1.48 1.62 4.46 3.00 2.19 -2.68 1.76 1.99 -0.86 -1.40 -1.50
Endosome 4.03 8.30 8.64 7.74 0.95 4.29 7.64 9.34 8.34 3.48 5.31 11.23 12.76 11.57 3.42
Golgi -13.05 -6.42 -3.84 7.43 1.98 -2.93 4.97 5.82 8.60 0.50 -11.05 -0.98 2.09 12.68 2.88
Lipid Particles -6.20 -2.27 0.13 6.37 2.45 1.36 7.66 8.23 7.15 2.06 -2.07 5.19 7.47 10.54 4.11
Mitochondria 8.50 4.08 0.85 -7.11 -3.05 -0.53 -10.23 -13.34 -12.90 -2.30 4.99 -4.36 -9.87 -15.75 -5.19
Mitotic Spindle -1.81 -2.74 -2.61 -2.01 -1.69 -1.99 -3.51 -0.85 1.63 3.29 -2.84 -4.40 -2.68 -0.84 1.05
None -7.85 -11.98 -15.00 -12.43 2.99 -3.95 -7.60 -14.55 -10.44 -8.94 -7.22 -12.83 -21.07 -16.69 1.39
Nuclear Periphery -3.64 -6.10 -6.48 -5.46 2.99 0.09 -2.67 -0.15 -0.65 2.94 -0.66 -6.26 -3.82 -5.00 4.69
Nuclear Periphery Foci -0.60 -4.23 -2.30 -1.77 3.40 0.20 -1.06 2.17 2.84 2.90 0.11 -3.79 1.18 1.49 4.73
Nucleolus 0.19 -2.30 -2.75 -3.21 0.88 -1.86 -1.14 -1.56 1.17 -0.75 -1.57 -2.55 -2.86 -0.41 0.76
Nucleus 1.41 1.38 1.28 -0.38 -0.15 -0.95 0.34 0.46 1.13 0.11 -0.34 1.09 1.18 1.01 0.14
Peroxisomes -0.52 4.99 6.52 9.28 2.12 3.06 10.60 12.40 10.13 2.22 2.93 11.61 13.89 13.89 3.10
Vacuole 0.94 -6.04 -3.85 -4.20 -0.35 -3.21 -4.84 -3.76 -0.64 -0.15 -2.39 -7.90 -5.26 -3.65 0.05
Vacuole Periphery 2.55 -8.67 -6.80 -7.69 0.68 -3.02 -7.27 -8.66 -6.09 -1.31 -0.08 -10.85 -10.02 -9.90 1.22