Standard name
Human Ortholog
Description Integral membrane protein of the Golgi; required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0.11 0.05 0 0.07 0.09 0.12 0 0 0 0 0 0
Bud 0 0 0 0 0 0.06 0.07 0.05 – 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.06 0.08 0 0.08 0.11 0.11 0.07 0.08 – 0.06 0.12 0.07 0 0 0.06 0.09 0.14 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0.05 0 0 – 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0 0 0
Endosome 0.23 0.2 0.06 0.14 0.19 0 0 0 – 0 0 0 0.23 0.21 0.26 0.18 0.11 0.1 0.23 0.19 0.26 0.19 0.2 0.16
Golgi 0.32 0.35 0.07 0.06 0 0 0 0 – 0 0 0 0.32 0.51 0.44 0.6 0.65 0.69 0.57 0.59 0.46 0.46 0.49 0.52
Mitochondria 0.65 0.59 0.88 0.78 0.73 0.87 0.89 0.85 – 0.9 0.88 0.91 0.4 0.25 0.2 0 0.05 0 0.08 0.12 0.13 0.18 0.17 0.15
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.07 0 0.09 0.07 0.07 0
SpindlePole 0 0 0 0 0.05 0 0 0.05 – 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.11 0.08 0 0 0.06 0 0 0.05 – 0.05 0 0 0.09 0.1 0.12 0.15 0.12 0.16 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 3 7 2 0 1 3 1 0 – 0 1 1 17 12 4 6 7 8 4 3 2 6 7 11
Bud 3 5 4 7 8 17 31 20 – 17 18 5 3 1 3 0 0 1 0 1 0 1 1 0
Bud Neck 0 0 0 0 0 0 0 0 – 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 2 4 8 – 7 5 6 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 1 1 0 0 1 0 2 – 1 0 1 1 0 1 0 1 0 0 0 0 0 0 0
Cytoplasm 15 17 5 16 31 30 31 33 – 19 33 20 6 9 16 7 11 4 0 1 1 1 0 1
Endoplasmic Reticulum 3 5 5 1 4 15 4 5 – 5 0 3 6 13 7 4 2 2 0 0 0 1 3 5
Endosome 61 42 9 28 51 3 5 13 – 3 1 4 34 49 67 15 9 7 67 47 42 47 52 42
Golgi 84 76 11 12 4 0 1 0 – 0 1 1 47 119 112 49 52 46 171 142 75 115 129 134
Mitochondria 169 127 131 154 197 241 377 335 – 265 240 246 60 58 52 2 4 3 24 28 20 45 45 37
Nucleus 0 0 0 1 5 2 2 4 – 3 0 1 1 1 2 0 0 0 0 0 0 2 0 0
Nuclear Periphery 0 1 2 0 0 0 1 0 – 1 0 1 1 2 1 0 0 0 0 0 0 0 0 0
Nucleolus 2 3 4 3 0 3 6 10 – 5 8 8 1 2 2 1 0 1 0 0 0 0 0 0
Peroxisomes 1 4 0 6 11 6 9 11 – 5 6 0 5 6 5 0 0 0 19 9 14 16 18 10
SpindlePole 8 5 2 4 14 6 14 21 – 10 5 5 8 8 11 1 0 2 1 1 0 1 0 1
Vac/Vac Membrane 28 18 4 7 17 6 11 19 – 16 11 8 14 24 30 12 10 11 6 5 1 7 5 9
Unique Cell Count 261 215 149 197 270 278 423 396 295 272 271 149 233 254 82 80 67 298 244 162 251 266 259
Labelled Cell Count 379 311 180 239 344 335 497 481 358 329 310 204 304 313 97 96 85 298 244 162 251 266 259


Early Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.6 7.6 5.4 5.7 5.4 5.3 4.8 5.1 – 4.5 5.0 4.7 7.6 7.8 7.6 10.3 10.0 10.6 8.1 8.1 8.6
Std Deviation (1e-4) 1.2 1.2 1.1 1.1 1.5 1.4 1.6 1.5 – 1.0 1.5 1.0 1.1 1.2 1.2 1.6 1.8 1.6 1.3 1.2 1.6
Intensity Change (Log2) – – – 0.07 0.01 -0.04 -0.17 -0.08 – -0.26 -0.11 -0.19 0.5 0.53 0.49 0.93 0.89 0.97 0.58 0.58 0.66


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 3.6 1.9 0 0 0 0
Bud 0 0 1.6 0 1.2 – 0 1.7 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Cytoplasm 1.8 2.8 2.7 1.7 2.0 – 1.4 3.0 1.7 0.3 0.3 1.3 0 2.9 0
Endoplasmic Reticulum 0 0 1.0 0 0 – 0 0 0 0.3 1.0 0 0 0 0
Endosome 2.4 3.6 0 0 -1.5 – 0 0 0 4.1 4.0 5.0 2.9 1.4 0
Golgi -0.5 -3.1 0 0 0 – 0 0 0 5.3 8.8 7.7 8.7 9.3 9.5
Mitochondria -2.4 -3.6 -0.4 0 -1.0 – 0 0.1 0 -8.6 -12.0 -13.1 -12.6 -12.2 -11.7
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
SpindlePole 0 2.0 0 0 2.0 – 0 0 0 1.9 0 0 0 0 0
Vacuole 0 1.6 0 0 1.1 – 1.3 0.7 0 2.4 2.8 3.2 3.4 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.2605 3.5425 3.3121 2.7314 3.1357 3.3529 4.2893 5.3098 4.7932 4.726 4.3072 4.9411 1.9734 3.2583 2.4086 2.7528 2.3231 2.6863
Actin 0.0098 0.0051 0.0076 0.0007 0.0026 0.0219 0.0256 0.0121 0.0056 0.0004 0.0232 0.0148 0.0081 0.0018 0.0066 0.0064 0.0011 0.0106
Bud 0.0001 0.0001 0.0002 0 0 0.0001 0.0002 0.0001 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0002 0 0
Bud Neck 0.0003 0.0002 0.0003 0.0001 0.0002 0.0003 0.0008 0.0002 0.0002 0.0001 0.0004 0.0002 0.0003 0.0002 0.0001 0.0002 0.0001 0.0002
Bud Periphery 0.0002 0.0001 0.0001 0 0 0.0001 0.0003 0.0001 0.0001 0 0.0002 0.0001 0.0001 0 0 0.0001 0 0.0001
Bud Site 0.0005 0.0002 0.0005 0.0001 0.0001 0.0001 0.0031 0.0003 0.0002 0 0.0009 0.0002 0.0002 0.0002 0.0001 0.0005 0.0001 0.0001
Cell Periphery 0.0001 0 0 0 0 0 0.0003 0 0 0 0.0001 0 0.0001 0 0 0 0 0
Cytoplasm 0.0011 0.0014 0.0019 0.0007 0.0009 0.0009 0.002 0.0014 0.0009 0.0005 0.0009 0.0009 0.0016 0.0019 0.0011 0.0013 0.0006 0.0006
Cytoplasmic Foci 0.1228 0.1384 0.1255 0.0964 0.1321 0.1017 0.1484 0.1464 0.1096 0.1299 0.1067 0.0834 0.1203 0.1488 0.1139 0.0959 0.1139 0.0909
Eisosomes 0.0001 0 0.0001 0 0 0 0.0008 0 0.0001 0 0.0001 0 0.0004 0 0 0 0 0
Endoplasmic Reticulum 0.0007 0.0002 0.0003 0.0002 0.0002 0.0002 0.0017 0.0002 0.0003 0.0001 0.0007 0.0004 0.0006 0.0003 0.0003 0.0004 0.0001 0.0003
Endosome 0.2143 0.2102 0.2109 0.2828 0.247 0.1418 0.1724 0.1544 0.1767 0.2486 0.2284 0.138 0.2149 0.1966 0.2071 0.2389 0.1875 0.1339
Golgi 0.4721 0.5596 0.5642 0.572 0.5474 0.5863 0.4134 0.5676 0.5255 0.5484 0.3304 0.5651 0.4759 0.5809 0.5441 0.5579 0.5784 0.6235
Lipid Particles 0.0461 0.0046 0.0122 0.0183 0.0151 0.0493 0.0701 0.0325 0.0282 0.0081 0.2183 0.0362 0.0434 0.0282 0.0216 0.015 0.0122 0.0271
Mitochondria 0.0652 0.0357 0.0326 0.0087 0.0033 0.0274 0.028 0.0259 0.0444 0.0048 0.0056 0.0358 0.0413 0.005 0.0302 0.0256 0.0337 0.0325
None 0.0003 0 0.0008 0 0.0001 0.0001 0.0139 0.0002 0.0008 0 0.0014 0.0001 0.0158 0.0002 0.0002 0.0001 0 0
Nuclear Periphery 0.001 0 0.0006 0 0.0001 0.0001 0.0021 0 0.0006 0 0.0018 0.0015 0.0017 0.0001 0.0001 0.0001 0 0
Nucleolus 0.0001 0 0.0008 0 0 0 0.0008 0 0.0001 0 0.0004 0.0003 0.001 0 0 0 0 0
Nucleus 0.0002 0 0.0004 0 0 0 0.001 0 0.0001 0 0.0003 0.0052 0.0012 0 0 0 0 0
Peroxisomes 0.0609 0.0414 0.0345 0.017 0.0481 0.068 0.107 0.0571 0.1028 0.0581 0.0686 0.1144 0.0685 0.0342 0.0726 0.0543 0.0707 0.0784
Punctate Nuclear 0.0006 0.0001 0.0047 0.0001 0.0002 0.0002 0.0058 0.0002 0.0016 0.0001 0.0085 0.0003 0.0024 0.0001 0.0001 0.0001 0.0001 0.0001
Vacuole 0.0016 0.0014 0.0007 0.0011 0.0014 0.0005 0.0009 0.0004 0.0009 0.0003 0.0012 0.0011 0.0011 0.0005 0.0007 0.0015 0.0005 0.0005
Vacuole Periphery 0.002 0.0011 0.0011 0.0017 0.0013 0.0009 0.0012 0.0007 0.0013 0.0006 0.0016 0.0018 0.0013 0.0007 0.001 0.0017 0.0009 0.001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 33.3545 29.0111 27.5073 28.7513 14.0256 36.039 36.6663 30.2467 30.7159 37.383
Translational Efficiency 1.0012 1.0356 0.8302 1.0819 1.9729 0.9551 0.8873 0.8306 1.0682 0.788

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
907 1330 2914 2042 1769 1910 3248 2501 2676 3240 6162 4543

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 654.95 878.72 965.57 922.00 736.14 857.19 979.87 995.71 708.62 866.03 973.11 962.58
Standard Deviation 82.11 121.95 128.29 110.21 125.68 110.03 123.61 126.19 119.18 115.55 126.04 124.78
Intensity Change Log 2 — 0.424019 0.559996 0.493382 — 0.219635 0.412610 0.435745 — 0.319476 0.483881 0.463169

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.002039 0.003504 0.001824 0.002140 0.000854 0.001036 0.001149 0.008034 0.001255 0.002049 0.001468 0.005385
Bud Neck 0.001567 0.000288 0.000253 0.000181 0.001502 0.000171 0.000052 0.000354 0.001524 0.000219 0.000147 0.000276
Bud Site 0.003655 0.001648 0.001214 0.001711 0.001749 0.000885 0.000681 0.003599 0.002395 0.001199 0.000933 0.002750
Cell Periphery 0.000160 0.000062 0.000024 0.000030 0.000104 0.000030 0.000018 0.000044 0.000123 0.000043 0.000021 0.000038
Cytoplasm 0.006006 0.002332 0.000643 0.001000 0.004412 0.001099 0.000428 0.003778 0.004952 0.001605 0.000530 0.002529
Cytoplasmic Foci 0.165941 0.241373* 0.271667* 0.269143* 0.183002* 0.205971* 0.251099* 0.225240* 0.177219 0.220503* 0.260826* 0.244974*
Eisosomes 0.000156 0.000068 0.000022 0.000025 0.000087 0.000041 0.000021 0.000053 0.000110 0.000052 0.000022 0.000041
Endoplasmic Reticulum 0.000210 0.000235 0.000264 0.000347 0.000421 0.000101 0.000115 0.000185 0.000349 0.000156 0.000186 0.000258
Endosome 0.125895 0.166758 0.266961* 0.227749* 0.184023 0.188667 0.228509* 0.299067* 0.164321 0.179673 0.246693* 0.267011*
Golgi 0.532025* 0.521333* 0.392740* 0.442941* 0.526948* 0.550367* 0.484677* 0.405852* 0.528668* 0.538449* 0.441200* 0.422523*
Lipid Particles 0.018580 0.007877 0.008210 0.005219 0.029934 0.005851 0.002569 0.003574 0.026086 0.006682 0.005236 0.004313
Mitochondria 0.094301 0.027225 0.010815 0.010730 0.038450 0.025169 0.014188 0.012036 0.057380 0.026013 0.012593 0.011449
Mitotic Spindle 0.003769 0.004345 0.020305 0.014812 0.002273 0.002578 0.004437 0.006711 0.002780 0.003303 0.011941 0.010352
None 0.000494 0.000030 0.000014 0.000013 0.001548 0.000042 0.000008 0.000015 0.001191 0.000037 0.000011 0.000014
Nuclear Periphery 0.000036 0.000052 0.000165 0.000077 0.000070 0.000020 0.000045 0.000056 0.000058 0.000033 0.000101 0.000066
Nuclear Periphery Foci 0.000217 0.000704 0.000981 0.000571 0.001811 0.000106 0.000202 0.000202 0.001271 0.000351 0.000570 0.000368
Nucleolus 0.000761 0.000025 0.000058 0.000018 0.001452 0.000388 0.000003 0.000028 0.001218 0.000239 0.000029 0.000023
Nucleus 0.000815 0.000040 0.000044 0.000091 0.001371 0.000122 0.000004 0.000202 0.001183 0.000088 0.000023 0.000152
Peroxisomes 0.035955 0.015318 0.003921 0.003879 0.014747 0.010099 0.002587 0.012325 0.021935 0.012241 0.003218 0.008529
Vacuole 0.003388 0.003195 0.013359 0.012856 0.002621 0.003297 0.005432 0.013589 0.002881 0.003255 0.009181 0.013260
Vacuole Periphery 0.004029 0.003590 0.006517 0.006468 0.002624 0.003961 0.003775 0.005055 0.003100 0.003809 0.005072 0.005690

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -1.72 0.41 -0.19 1.48 -0.52 -0.79 -1.17 -6.64 -6.46 -6.33 -2.13 -0.82 -6.42 -4.69 -5.93
Bud Neck 4.40 4.54 4.81 2.00 1.79 5.00 5.52 4.27 -2.64 -5.57 6.47 6.89 6.18 -1.34 -3.73
Bud Site 1.80 2.21 1.73 -0.21 -1.73 2.09 2.99 -3.97 -6.79 -8.47 2.58 3.30 -0.81 -5.86 -7.93
Cell Periphery 4.54 6.56 6.29 4.44 -2.21 5.82 6.85 4.21 -2.07 -3.98 7.10 9.37 7.43 1.07 -4.40
Cytoplasm 3.22 5.24 4.59 1.91 -2.10 3.92 4.83 0.53 -4.73 -6.20 4.87 6.83 3.13 -2.68 -6.52
Cytoplasmic Foci -12.72 -20.00 -18.47 -4.81 0.53 -4.88 -15.45 -9.33 -4.28 5.97 -11.76 -24.67 -19.16 -6.80 4.81
Eisosomes 6.92 12.81 12.42 5.55 -1.54 4.30 6.35 3.11 -2.78 -8.87 6.92 11.45 8.73 2.94 -8.67
Endoplasmic Reticulum -0.55 -1.30 -2.78 -2.03 -1.32 2.01 1.93 1.46 -2.76 -2.15 1.81 1.50 0.82 -3.43 -2.16
Endosome -6.65 -23.11 -16.51 -10.90 7.11 -0.92 -8.98 -19.95 -19.94 -13.88 -3.76 -20.79 -23.84 -21.83 -5.32
Golgi 0.95 14.28 8.86 9.88 -7.43 -3.24 5.85 16.16 21.37 13.26 -1.76 15.23 17.78 22.49 4.22
Lipid Particles 4.07 4.28 5.52 1.82 2.95 8.20 9.55 9.10 2.44 -1.65 9.04 10.04 10.43 2.90 1.53
Mitochondria 10.95 14.83 14.65 5.91 0.07 4.28 8.78 9.77 6.49 1.44 10.57 16.81 17.13 8.79 1.11
Mitotic Spindle -0.47 -8.53 -5.51 -5.49 2.10 -0.41 -2.56 -4.14 -4.10 -2.27 -0.78 -9.15 -7.11 -6.91 1.09
None 2.27 2.35 2.35 4.01 0.75 2.71 2.77 2.76 2.16 -4.06 3.08 3.15 3.14 2.95 -2.65
Nuclear Periphery -0.56 -7.89 -3.13 -0.97 4.71 1.87 0.98 0.41 -1.42 -0.48 1.21 -2.25 -0.37 -1.82 2.12
Nuclear Periphery Foci -1.35 -5.63 -2.13 0.34 2.13 3.25 3.21 3.20 -0.41 -0.08 2.52 2.16 2.72 0.07 2.02
Nucleolus 1.28 1.15 1.29 0.61 1.13 1.89 3.48 3.42 1.28 -1.77 2.42 3.39 3.48 1.30 0.73
Nucleus 2.18 2.14 1.91 -0.90 -0.74 2.95 3.36 2.62 -0.33 -1.18 3.64 3.93 3.26 -0.59 -1.37
Peroxisomes 6.02 10.51 10.51 6.76 0.19 3.09 10.38 1.57 -1.58 -9.14 6.22 14.31 9.42 3.42 -8.02
Vacuole 0.25 -10.64 -8.70 -10.21 0.27 -1.56 -6.38 -11.32 -10.11 -7.98 -0.95 -13.76 -14.81 -14.22 -5.56
Vacuole Periphery 0.73 -4.66 -4.12 -4.55 0.09 -3.11 -3.98 -5.81 -2.00 -2.88 -2.06 -7.17 -7.65 -4.55 -1.73
Standard name
Human Ortholog
Description Integral membrane protein of the Golgi; required for targeting of the Arf-like GTPase Arl3p to the Golgi; multicopy suppressor of ypt6 null mutation
Localization
Cell Percentages Golgi (6%)
Cell Cycle Regulation No
Subcompartmental Group N/A

Sys1

Sys1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sys1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available