Standard name
Human Ortholog
Description bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 – – 0.27 0.85 0.88 0.82 0.59 0.67 0.13 0.55 0.43 0.44 0.34 0.82 0.69 0.54 0.96 0.9 0.81 0.77 0.87 0.86
Endoplasmic Reticulum 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 – – 0.14 0 0 0 0 0 0 0 0.06 0.06 0.12 0 0.06 0.07 0 0 0 0 0 0
Golgi 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – – 0.2 0.21 0.42 0.34 0.52 0.5 0.72 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – – 0.22 0 0 0 0 0 0.33 0 0.09 0.12 0.16 0 0.11 0.1 0 0 0 0.07 0 0
Nuclear Periphery 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – 0.33 0.08 0 0 0.15 0 0.06 0.05 0.52 0.49 0.48 0.14 0.23 0.38 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 2
Bud 1 0 – – 0 2 1 1 1 3 0 1 1 0 0 0 0 0 1 0 0 3 5 7
Bud Neck 0 0 – – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 – – 0 0 0 0 1 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 – – 0 0 1 1 3 0 1 6 0 0 1 0 3 0 0 0 0 0 0 0
Cytoplasm 318 131 – – 21 41 114 93 52 84 11 52 153 157 138 133 84 55 310 123 46 188 291 341
Endoplasmic Reticulum 3 1 – – 1 0 1 0 1 0 1 2 6 1 0 8 1 3 0 1 0 1 5 7
Endosome 0 0 – – 11 0 0 0 0 0 0 0 21 21 47 7 7 7 2 2 0 6 6 7
Golgi 0 0 – – 0 0 0 1 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0
Mitochondria 8 4 – – 16 10 54 38 46 62 63 50 1 2 5 2 3 0 1 0 1 0 0 2
Nucleus 1 1 – – 17 1 1 5 2 0 29 2 32 44 64 6 14 10 0 1 0 16 7 2
Nuclear Periphery 0 0 – – 0 0 1 0 0 0 3 2 0 0 0 1 0 0 0 0 0 2 0 1
Nucleolus 0 1 – – 0 0 1 0 0 0 8 2 2 1 2 0 0 0 0 0 0 2 2 1
Peroxisomes 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 7 3 2
Vac/Vac Membrane 3 0 – – 26 4 3 1 13 3 5 5 188 175 194 23 28 38 1 2 1 9 7 14
Unique Cell Count 329 137 79 48 130 113 88 125 87 94 359 355 405 163 122 101 325 137 58 243 336 397
Labelled Cell Count 336 140 92 58 177 140 120 152 124 122 404 402 451 180 141 114 325 137 58 243 336 397


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.9 – – 5.8 5.7 3.9 4.3 4.5 4.0 4.3 4.0 7.4 7.2 7.1 7.2 8.3 9.0 6.8 7.2 7.7
Std Deviation (1e-4) 0.7 1.0 – – 1.6 2.6 1.0 1.4 1.5 1.3 1.4 1.1 1.3 1.1 1.3 1.5 1.4 1.5 1.1 1.5 1.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.5148 6.2761 5.8127 5.0623 5.5552 5.8574 4.0774 5.111 4.7161 3.9402 3.6479 4.4908 5.8949 6.8179 6.0302 6.1281 6.0807 6.3092
Actin 0.0283 0.0005 0.0142 0.0288 0.0014 0.0044 0.0185 0.0006 0.0163 0.0066 0.0019 0.0028 0.0544 0.0025 0.0039 0.0046 0.0073 0.0175
Bud 0.0011 0.0002 0.0004 0.0006 0.0011 0.0004 0.0008 0.0005 0.001 0.0011 0.0004 0.0007 0.0016 0.0039 0.0007 0.0006 0.0002 0.0005
Bud Neck 0.0074 0.0006 0.001 0.0022 0.002 0.0095 0.0012 0.0013 0.0009 0.0025 0.0019 0.0045 0.0066 0.0007 0.001 0.0036 0.0042 0.0038
Bud Periphery 0.0012 0.0001 0.0003 0.0007 0.0006 0.0003 0.0007 0.0001 0.0013 0.0012 0.0004 0.0005 0.0017 0.0017 0.0005 0.0008 0.0002 0.0004
Bud Site 0.0046 0.0008 0.0012 0.0012 0.0022 0.0005 0.0034 0.0065 0.0054 0.0049 0.0007 0.0008 0.0195 0.0032 0.0013 0.0025 0.0013 0.0007
Cell Periphery 0.0004 0 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0006 0.0001 0.0001 0.0006 0.0001 0.0011 0.0002 0.0001 0.0001
Cytoplasm 0.5677 0.76 0.6339 0.5132 0.7101 0.6621 0.5327 0.698 0.4753 0.4012 0.6185 0.5988 0.5856 0.7419 0.5622 0.4631 0.5918 0.6221
Cytoplasmic Foci 0.0254 0.0031 0.0066 0.0205 0.0375 0.0112 0.0408 0.0044 0.0159 0.0077 0.0235 0.0114 0.0283 0.0047 0.0053 0.0098 0.0245 0.0088
Eisosomes 0.0002 0 0.0002 0.0001 0 0 0.0002 0 0.0001 0.0001 0 0.0001 0.0008 0 0.0048 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0104 0.0022 0.0045 0.0072 0.003 0.0065 0.0176 0.0027 0.0041 0.0053 0.0181 0.0041 0.0079 0.0035 0.0044 0.0015 0.0038 0.0037
Endosome 0.0467 0.0038 0.0102 0.0464 0.0268 0.0208 0.0264 0.0029 0.0146 0.0533 0.0826 0.011 0.0294 0.0024 0.0099 0.0046 0.0109 0.0052
Golgi 0.0122 0.0003 0.0036 0.0231 0.0105 0.0026 0.0099 0.0002 0.005 0.0151 0.0097 0.0082 0.0122 0.0002 0.0012 0.0034 0.0071 0.0045
Lipid Particles 0.0241 0 0.0024 0.0068 0.0005 0.0032 0.0072 0.0001 0.0029 0.0019 0.0111 0.0056 0.007 0.0001 0.0015 0.0012 0.0386 0.0066
Mitochondria 0.0201 0.0004 0.0157 0.0107 0.0303 0.0021 0.0172 0.0004 0.0027 0.0045 0.0053 0.0079 0.0129 0.0004 0.0047 0.0104 0.0073 0.0019
None 0.01 0.0036 0.0093 0.01 0.0093 0.0049 0.0437 0.0194 0.0187 0.0053 0.0096 0.0294 0.0135 0.0113 0.0137 0.0028 0.0074 0.0134
Nuclear Periphery 0.0226 0.0061 0.0163 0.0122 0.0112 0.0119 0.0171 0.0059 0.0106 0.0104 0.0181 0.0065 0.0127 0.0087 0.0117 0.0068 0.0086 0.009
Nucleolus 0.0032 0.0004 0.0006 0.0008 0.0003 0.0007 0.0007 0.001 0.0012 0.0008 0.0005 0.0017 0.0011 0.0005 0.0008 0.0006 0.0019 0.0008
Nucleus 0.1793 0.2132 0.2655 0.2701 0.1384 0.2453 0.2339 0.2357 0.381 0.4599 0.1667 0.2925 0.1654 0.1943 0.3519 0.4246 0.237 0.2791
Peroxisomes 0.0076 0.0001 0.0019 0.0061 0.0055 0.0024 0.007 0.0002 0.0025 0.0005 0.0019 0.0038 0.0142 0.0004 0.0003 0.0369 0.0277 0.0055
Punctate Nuclear 0.0163 0.003 0.0101 0.0345 0.0059 0.0069 0.0169 0.0184 0.0379 0.0071 0.0216 0.0072 0.0197 0.0183 0.0169 0.021 0.0173 0.015
Vacuole 0.0072 0.0013 0.0017 0.0029 0.0023 0.0033 0.0028 0.0015 0.0023 0.0071 0.0059 0.0022 0.0038 0.0009 0.0018 0.0009 0.0021 0.0012
Vacuole Periphery 0.0038 0.0002 0.0005 0.0018 0.001 0.0008 0.0009 0.0002 0.0003 0.0028 0.0015 0.0004 0.0012 0.0002 0.0004 0.0002 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5943 12.8276 6.0118 13.9096 12.7296 9.0579 15.7005 17.5077 16.5943 17.5465
Translational Efficiency 1.1203 1.1778 2.6113 1.0483 1.4622 2.3873 1.1572 1.1512 1.1005 1.0264

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
647 1384 1545 1369 356 1584 262 510 1003 2968 1807 1879

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 779.07 821.43 852.37 992.80 903.84 903.50 1145.40 1014.64 823.36 865.23 894.86 998.73
Standard Deviation 90.74 147.51 150.53 138.38 103.45 127.57 111.89 132.15 112.58 143.21 178.42 137.06
Intensity Change Log 2 — 0.076385 0.129727 0.349750 — -0.000543 0.341712 0.166829 — 0.035580 0.247432 0.254399

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000094 0.000218 0.000609 0.000719 0.000209 0.000623 0.001647 0.001475 0.000135 0.000434 0.000759 0.000924
Bud Neck 0.009574 0.003352 0.006884 0.021570 0.002626 0.004649 0.003811 0.018092 0.007108 0.004044 0.006439 0.020626
Bud Site 0.009622 0.013212 0.004296 0.026241 0.004958 0.017263 0.003901 0.045309 0.007967 0.015374 0.004238 0.031416
Cell Periphery 0.000131 0.000089 0.000152 0.000210 0.000262 0.000086 0.000227 0.000198 0.000178 0.000088 0.000163 0.000206
Cytoplasm 0.669630* 0.701253* 0.301743* 0.243018* 0.628882* 0.654228* 0.160237* 0.333393* 0.655167* 0.676156* 0.281226* 0.267548*
Cytoplasmic Foci 0.151654 0.131884 0.002450 0.005079 0.186401* 0.190372* 0.002159 0.009245 0.163987 0.163099 0.002407 0.006210
Eisosomes 0.000085 0.000026 0.000014 0.000026 0.000091 0.000040 0.000079 0.000043 0.000087 0.000034 0.000023 0.000031
Endoplasmic Reticulum 0.000512 0.001500 0.003081 0.005246 0.001895 0.000578 0.013762 0.005187 0.001003 0.001008 0.004630 0.005230
Endosome 0.008183 0.015772 0.000757 0.005642 0.016901 0.017528 0.003363 0.003728 0.011277 0.016709 0.001135 0.005122
Golgi 0.004024 0.003423 0.000203 0.011729 0.001198 0.007303 0.000182 0.001210 0.003021 0.005494 0.000200 0.008874
Lipid Particles 0.002157 0.001863 0.000360 0.000697 0.005919 0.001439 0.002459 0.001096 0.003492 0.001637 0.000664 0.000805
Mitochondria 0.001094 0.002913 0.001206 0.003682 0.000273 0.003283 0.001013 0.002939 0.000803 0.003111 0.001178 0.003481
Mitotic Spindle 0.002596 0.001529 0.010387 0.103934* 0.000249 0.006805 0.004880 0.068013 0.001763 0.004344 0.009589 0.094184
None 0.005911 0.002892 0.017099 0.020517 0.001987 0.000868 0.014896 0.029770 0.004518 0.001812 0.016780 0.023028
Nuclear Periphery 0.000082 0.000229 0.000945 0.003551 0.000425 0.000131 0.002523 0.001987 0.000203 0.000177 0.001174 0.003127
Nuclear Periphery Foci 0.000432 0.001737 0.001707 0.003665 0.004969 0.000730 0.006165 0.001775 0.002043 0.001199 0.002353 0.003152
Nucleolus 0.000479 0.002150 0.001753 0.001392 0.000670 0.000444 0.000909 0.001265 0.000547 0.001239 0.001631 0.001358
Nucleus 0.052462 0.028618 0.633195* 0.500890* 0.076056 0.014020 0.672469* 0.399353* 0.060836 0.020827 0.638889* 0.473331*
Peroxisomes 0.001946 0.001859 0.000234 0.000548 0.000755 0.002909 0.000307 0.001224 0.001523 0.002420 0.000244 0.000731
Vacuole 0.078372 0.082149 0.012701 0.037806 0.064468 0.074569 0.104792 0.072555 0.073437 0.078104 0.026054 0.047238
Vacuole Periphery 0.000961 0.003331 0.000224 0.003837 0.000804 0.002133 0.000219 0.002142 0.000905 0.002692 0.000223 0.003377

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -4.62 -15.69 -18.51 -14.78 -4.82 -1.94 -8.07 -9.47 -3.64 3.43 -2.75 -15.47 -19.88 -6.88 -2.35
Bud Neck 3.98 -0.06 -4.97 -11.64 -5.77 -2.23 -4.41 -6.66 -6.19 -4.27 2.93 -1.56 -7.31 -12.79 -6.12
Bud Site -2.35 3.48 -2.11 -0.10 -5.98 -9.08 -2.18 -4.41 -0.28 -3.66 -6.56 2.85 -4.53 0.49 -7.09
Cell Periphery 3.98 -2.41 -4.54 -10.11 -1.95 9.62 0.36 4.13 -7.87 3.96 9.44 0.50 -0.12 -12.92 -0.75
Cytoplasm -2.65 37.89 53.50 65.02* 14.15 -2.29 35.70 28.90 39.63 -6.67 -2.58 45.76 60.74 79.14* 10.85
Cytoplasmic Foci 2.69 24.23 24.31 31.76 1.22 -0.49 23.56 23.23 40.15 -2.02 0.15 33.15 33.11 50.95 -0.33
Eisosomes 9.21 10.51 5.31 -10.38 -15.41 8.21 -2.37 3.10 -7.73 5.45 11.47 11.34 6.56 -10.74 -10.07
Endoplasmic Reticulum -2.84 -16.36 -22.78 -14.89 -11.24 5.48 -14.59 -11.17 -14.24 8.12 0.13 -18.03 -24.45 -22.74 -5.76
Endosome -5.91 9.63 7.61 13.05 -4.38 -0.02 7.08 8.32 14.41 4.66 -4.70 12.28 11.08 19.27 -3.44
Golgi 0.91 7.65 1.75 1.21 -1.86 -7.18 3.33 2.84 8.59 -0.96 -4.14 8.26 1.75 4.39 -1.90
Lipid Particles 1.05 7.91 4.46 3.33 -11.54 5.51 2.58 5.16 -1.70 2.55 5.51 7.61 6.58 2.34 -3.82
Mitochondria -1.84 -0.81 -7.01 -0.07 -6.75 -3.68 -11.87 -7.74 1.23 -3.11 -3.60 -2.63 -10.07 0.52 -6.96
Mitotic Spindle 0.59 -4.57 -11.88 -12.51 -9.61 -4.16 -3.78 -8.02 -6.92 -5.35 -2.04 -6.13 -14.37 -13.80 -11.04
None 2.70 -5.96 -4.25 -8.61 1.73 4.53 -2.45 -6.66 -7.35 -3.38 3.85 -7.82 -7.32 -12.06 0.43
Nuclear Periphery -2.34 -22.94 -23.83 -22.42 -18.10 2.58 -14.23 -15.27 -17.72 -0.21 0.62 -20.40 -27.48 -27.75 -18.43
Nuclear Periphery Foci -8.09 -7.84 -4.42 -0.75 0.11 6.67 -1.46 4.00 -5.01 4.07 3.61 -1.86 0.16 -3.15 1.95
Nucleolus -2.28 -4.25 -7.12 0.10 -0.23 1.25 -0.28 -1.45 -2.26 -1.44 -1.55 -3.55 -4.83 -1.96 -0.70
Nucleus 6.78 -77.64* -68.85* -73.58* 2.51 11.10 -29.60 -30.54 -37.82 5.19 13.81 -81.53 -72.67* -82.91* 5.61
Peroxisomes 0.16 3.72 3.65 3.54 -0.53 -6.08 2.35 1.46 6.61 -0.38 -2.09 4.31 4.02 7.72 -0.96
Vacuole -1.07 14.20 -0.93 0.02 -14.45 -1.57 -7.23 -7.84 -7.69 1.23 -1.41 7.97 -5.46 -4.99 -12.48
Vacuole Periphery -5.14 8.81 0.63 4.75 -1.97 -4.08 4.50 1.38 4.45 -0.83 -6.49 9.71 0.96 5.80 -2.16
Standard name
Human Ortholog
Description bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p
Localization
Cell Percentages cytoplasm (97%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Rtg3

Rtg3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Rtg3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available