Standard name | |
---|---|
Human Ortholog | |
Description | bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 2 |
Bud | 1 | 0 | – | – | 0 | 2 | 1 | 1 | 1 | 3 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 3 | 5 | 7 |
Bud Neck | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Bud Site | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 2 | – | – | 0 | 0 | 1 | 1 | 3 | 0 | 1 | 6 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 318 | 131 | – | – | 21 | 41 | 114 | 93 | 52 | 84 | 11 | 52 | 153 | 157 | 138 | 133 | 84 | 55 | 310 | 123 | 46 | 188 | 291 | 341 |
Endoplasmic Reticulum | 3 | 1 | – | – | 1 | 0 | 1 | 0 | 1 | 0 | 1 | 2 | 6 | 1 | 0 | 8 | 1 | 3 | 0 | 1 | 0 | 1 | 5 | 7 |
Endosome | 0 | 0 | – | – | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 21 | 21 | 47 | 7 | 7 | 7 | 2 | 2 | 0 | 6 | 6 | 7 |
Golgi | 0 | 0 | – | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 8 | 4 | – | – | 16 | 10 | 54 | 38 | 46 | 62 | 63 | 50 | 1 | 2 | 5 | 2 | 3 | 0 | 1 | 0 | 1 | 0 | 0 | 2 |
Nucleus | 1 | 1 | – | – | 17 | 1 | 1 | 5 | 2 | 0 | 29 | 2 | 32 | 44 | 64 | 6 | 14 | 10 | 0 | 1 | 0 | 16 | 7 | 2 |
Nuclear Periphery | 0 | 0 | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 2 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 |
Nucleolus | 0 | 1 | – | – | 0 | 0 | 1 | 0 | 0 | 0 | 8 | 2 | 2 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 |
Peroxisomes | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | – | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 7 | 3 | 2 |
Vac/Vac Membrane | 3 | 0 | – | – | 26 | 4 | 3 | 1 | 13 | 3 | 5 | 5 | 188 | 175 | 194 | 23 | 28 | 38 | 1 | 2 | 1 | 9 | 7 | 14 |
Unique Cell Count | 329 | 137 | 79 | 48 | 130 | 113 | 88 | 125 | 87 | 94 | 359 | 355 | 405 | 163 | 122 | 101 | 325 | 137 | 58 | 243 | 336 | 397 | ||
Labelled Cell Count | 336 | 140 | 92 | 58 | 177 | 140 | 120 | 152 | 124 | 122 | 404 | 402 | 451 | 180 | 141 | 114 | 325 | 137 | 58 | 243 | 336 | 397 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.8 | 6.9 | – | – | 5.8 | 5.7 | 3.9 | 4.3 | 4.5 | 4.0 | 4.3 | 4.0 | 7.4 | 7.2 | 7.1 | 7.2 | 8.3 | 9.0 | 6.8 | 7.2 | 7.7 |
Std Deviation (1e-4) | 0.7 | 1.0 | – | – | 1.6 | 2.6 | 1.0 | 1.4 | 1.5 | 1.3 | 1.4 | 1.1 | 1.3 | 1.1 | 1.3 | 1.5 | 1.4 | 1.5 | 1.1 | 1.5 | 1.7 |
Intensity Change (Log2) | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.5148 | 6.2761 | 5.8127 | 5.0623 | 5.5552 | 5.8574 | 4.0774 | 5.111 | 4.7161 | 3.9402 | 3.6479 | 4.4908 | 5.8949 | 6.8179 | 6.0302 | 6.1281 | 6.0807 | 6.3092 |
Actin | 0.0283 | 0.0005 | 0.0142 | 0.0288 | 0.0014 | 0.0044 | 0.0185 | 0.0006 | 0.0163 | 0.0066 | 0.0019 | 0.0028 | 0.0544 | 0.0025 | 0.0039 | 0.0046 | 0.0073 | 0.0175 |
Bud | 0.0011 | 0.0002 | 0.0004 | 0.0006 | 0.0011 | 0.0004 | 0.0008 | 0.0005 | 0.001 | 0.0011 | 0.0004 | 0.0007 | 0.0016 | 0.0039 | 0.0007 | 0.0006 | 0.0002 | 0.0005 |
Bud Neck | 0.0074 | 0.0006 | 0.001 | 0.0022 | 0.002 | 0.0095 | 0.0012 | 0.0013 | 0.0009 | 0.0025 | 0.0019 | 0.0045 | 0.0066 | 0.0007 | 0.001 | 0.0036 | 0.0042 | 0.0038 |
Bud Periphery | 0.0012 | 0.0001 | 0.0003 | 0.0007 | 0.0006 | 0.0003 | 0.0007 | 0.0001 | 0.0013 | 0.0012 | 0.0004 | 0.0005 | 0.0017 | 0.0017 | 0.0005 | 0.0008 | 0.0002 | 0.0004 |
Bud Site | 0.0046 | 0.0008 | 0.0012 | 0.0012 | 0.0022 | 0.0005 | 0.0034 | 0.0065 | 0.0054 | 0.0049 | 0.0007 | 0.0008 | 0.0195 | 0.0032 | 0.0013 | 0.0025 | 0.0013 | 0.0007 |
Cell Periphery | 0.0004 | 0 | 0.0001 | 0.0002 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0006 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0.0011 | 0.0002 | 0.0001 | 0.0001 |
Cytoplasm | 0.5677 | 0.76 | 0.6339 | 0.5132 | 0.7101 | 0.6621 | 0.5327 | 0.698 | 0.4753 | 0.4012 | 0.6185 | 0.5988 | 0.5856 | 0.7419 | 0.5622 | 0.4631 | 0.5918 | 0.6221 |
Cytoplasmic Foci | 0.0254 | 0.0031 | 0.0066 | 0.0205 | 0.0375 | 0.0112 | 0.0408 | 0.0044 | 0.0159 | 0.0077 | 0.0235 | 0.0114 | 0.0283 | 0.0047 | 0.0053 | 0.0098 | 0.0245 | 0.0088 |
Eisosomes | 0.0002 | 0 | 0.0002 | 0.0001 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.0008 | 0 | 0.0048 | 0.0001 | 0.0002 | 0.0001 |
Endoplasmic Reticulum | 0.0104 | 0.0022 | 0.0045 | 0.0072 | 0.003 | 0.0065 | 0.0176 | 0.0027 | 0.0041 | 0.0053 | 0.0181 | 0.0041 | 0.0079 | 0.0035 | 0.0044 | 0.0015 | 0.0038 | 0.0037 |
Endosome | 0.0467 | 0.0038 | 0.0102 | 0.0464 | 0.0268 | 0.0208 | 0.0264 | 0.0029 | 0.0146 | 0.0533 | 0.0826 | 0.011 | 0.0294 | 0.0024 | 0.0099 | 0.0046 | 0.0109 | 0.0052 |
Golgi | 0.0122 | 0.0003 | 0.0036 | 0.0231 | 0.0105 | 0.0026 | 0.0099 | 0.0002 | 0.005 | 0.0151 | 0.0097 | 0.0082 | 0.0122 | 0.0002 | 0.0012 | 0.0034 | 0.0071 | 0.0045 |
Lipid Particles | 0.0241 | 0 | 0.0024 | 0.0068 | 0.0005 | 0.0032 | 0.0072 | 0.0001 | 0.0029 | 0.0019 | 0.0111 | 0.0056 | 0.007 | 0.0001 | 0.0015 | 0.0012 | 0.0386 | 0.0066 |
Mitochondria | 0.0201 | 0.0004 | 0.0157 | 0.0107 | 0.0303 | 0.0021 | 0.0172 | 0.0004 | 0.0027 | 0.0045 | 0.0053 | 0.0079 | 0.0129 | 0.0004 | 0.0047 | 0.0104 | 0.0073 | 0.0019 |
None | 0.01 | 0.0036 | 0.0093 | 0.01 | 0.0093 | 0.0049 | 0.0437 | 0.0194 | 0.0187 | 0.0053 | 0.0096 | 0.0294 | 0.0135 | 0.0113 | 0.0137 | 0.0028 | 0.0074 | 0.0134 |
Nuclear Periphery | 0.0226 | 0.0061 | 0.0163 | 0.0122 | 0.0112 | 0.0119 | 0.0171 | 0.0059 | 0.0106 | 0.0104 | 0.0181 | 0.0065 | 0.0127 | 0.0087 | 0.0117 | 0.0068 | 0.0086 | 0.009 |
Nucleolus | 0.0032 | 0.0004 | 0.0006 | 0.0008 | 0.0003 | 0.0007 | 0.0007 | 0.001 | 0.0012 | 0.0008 | 0.0005 | 0.0017 | 0.0011 | 0.0005 | 0.0008 | 0.0006 | 0.0019 | 0.0008 |
Nucleus | 0.1793 | 0.2132 | 0.2655 | 0.2701 | 0.1384 | 0.2453 | 0.2339 | 0.2357 | 0.381 | 0.4599 | 0.1667 | 0.2925 | 0.1654 | 0.1943 | 0.3519 | 0.4246 | 0.237 | 0.2791 |
Peroxisomes | 0.0076 | 0.0001 | 0.0019 | 0.0061 | 0.0055 | 0.0024 | 0.007 | 0.0002 | 0.0025 | 0.0005 | 0.0019 | 0.0038 | 0.0142 | 0.0004 | 0.0003 | 0.0369 | 0.0277 | 0.0055 |
Punctate Nuclear | 0.0163 | 0.003 | 0.0101 | 0.0345 | 0.0059 | 0.0069 | 0.0169 | 0.0184 | 0.0379 | 0.0071 | 0.0216 | 0.0072 | 0.0197 | 0.0183 | 0.0169 | 0.021 | 0.0173 | 0.015 |
Vacuole | 0.0072 | 0.0013 | 0.0017 | 0.0029 | 0.0023 | 0.0033 | 0.0028 | 0.0015 | 0.0023 | 0.0071 | 0.0059 | 0.0022 | 0.0038 | 0.0009 | 0.0018 | 0.0009 | 0.0021 | 0.0012 |
Vacuole Periphery | 0.0038 | 0.0002 | 0.0005 | 0.0018 | 0.001 | 0.0008 | 0.0009 | 0.0002 | 0.0003 | 0.0028 | 0.0015 | 0.0004 | 0.0012 | 0.0002 | 0.0004 | 0.0002 | 0.0004 | 0.0003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.5943 | 12.8276 | 6.0118 | 13.9096 | 12.7296 | 9.0579 | 15.7005 | 17.5077 | 16.5943 | 17.5465 |
Translational Efficiency | 1.1203 | 1.1778 | 2.6113 | 1.0483 | 1.4622 | 2.3873 | 1.1572 | 1.1512 | 1.1005 | 1.0264 |
Dataset | Hit |
---|---|
Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |