Standard name
Human Ortholog
Description bHLH/Zip transcription factor for retrograde (RTG) and TOR pathways; forms a complex with another bHLH/Zip protein, Rtg1p, to activate the pathways; target of Hog1p
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 – – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.96 – – 0.27 0.85 0.88 0.82 0.59 0.67 0.13 0.55 0.43 0.44 0.34 0.82 0.69 0.54 0.96 0.9 0.81 0.77 0.87 0.86
Endoplasmic Reticulum 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Endosome 0 0 – – 0.14 0 0 0 0 0 0 0 0.06 0.06 0.12 0 0.06 0.07 0 0 0 0 0 0
Golgi 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 – – 0.2 0.21 0.42 0.34 0.52 0.5 0.72 0.53 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 – – 0.22 0 0 0 0 0 0.33 0 0.09 0.12 0.16 0 0.11 0.1 0 0 0 0.07 0 0
Nuclear Periphery 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 – – 0 0 0 0 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 – – 0.33 0.08 0 0 0.15 0 0.06 0.05 0.52 0.49 0.48 0.14 0.23 0.38 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0 2
Bud 1 0 – – 0 2 1 1 1 3 0 1 1 0 0 0 0 0 1 0 0 3 5 7
Bud Neck 0 0 – – 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0
Bud Site 0 0 – – 0 0 0 0 1 0 2 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 2 – – 0 0 1 1 3 0 1 6 0 0 1 0 3 0 0 0 0 0 0 0
Cytoplasm 318 131 – – 21 41 114 93 52 84 11 52 153 157 138 133 84 55 310 123 46 188 291 341
Endoplasmic Reticulum 3 1 – – 1 0 1 0 1 0 1 2 6 1 0 8 1 3 0 1 0 1 5 7
Endosome 0 0 – – 11 0 0 0 0 0 0 0 21 21 47 7 7 7 2 2 0 6 6 7
Golgi 0 0 – – 0 0 0 1 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0
Mitochondria 8 4 – – 16 10 54 38 46 62 63 50 1 2 5 2 3 0 1 0 1 0 0 2
Nucleus 1 1 – – 17 1 1 5 2 0 29 2 32 44 64 6 14 10 0 1 0 16 7 2
Nuclear Periphery 0 0 – – 0 0 1 0 0 0 3 2 0 0 0 1 0 0 0 0 0 2 0 1
Nucleolus 0 1 – – 0 0 1 0 0 0 8 2 2 1 2 0 0 0 0 0 0 2 2 1
Peroxisomes 0 0 – – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 – – 0 0 0 0 0 0 1 0 0 1 0 0 0 1 0 0 0 7 3 2
Vac/Vac Membrane 3 0 – – 26 4 3 1 13 3 5 5 188 175 194 23 28 38 1 2 1 9 7 14
Unique Cell Count 329 137 79 48 130 113 88 125 87 94 359 355 405 163 122 101 325 137 58 243 336 397
Labelled Cell Count 336 140 92 58 177 140 120 152 124 122 404 402 451 180 141 114 325 137 58 243 336 397


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.8 6.9 – – 5.8 5.7 3.9 4.3 4.5 4.0 4.3 4.0 7.4 7.2 7.1 7.2 8.3 9.0 6.8 7.2 7.7
Std Deviation (1e-4) 0.7 1.0 – – 1.6 2.6 1.0 1.4 1.5 1.3 1.4 1.1 1.3 1.1 1.3 1.5 1.4 1.5 1.1 1.5 1.7
Intensity Change (Log2) – – – – – – – – – – – – – – – – – – – – –


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – – –
Bud Neck – – – – – – – – – – – – – – –
Bud Site – – – – – – – – – – – – – – –
Cell Periphery – – – – – – – – – – – – – – –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – – –
Endosome – – – – – – – – – – – – – – –
Golgi – – – – – – – – – – – – – – –
Mitochondria – – – – – – – – – – – – – – –
Nuclear Periphery – – – – – – – – – – – – – – –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – – –
Peroxisomes – – – – – – – – – – – – – – –
SpindlePole – – – – – – – – – – – – – – –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – – –
Cytoplasm – – – – – – – – – – – – – – –
Nucleus – – – – – – – – – – – – – – –
Vacuole – – – – – – – – – – – – – – –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.5148 6.2761 5.8127 5.0623 5.5552 5.8574 4.0774 5.111 4.7161 3.9402 3.6479 4.4908 5.8949 6.8179 6.0302 6.1281 6.0807 6.3092
Actin 0.0283 0.0005 0.0142 0.0288 0.0014 0.0044 0.0185 0.0006 0.0163 0.0066 0.0019 0.0028 0.0544 0.0025 0.0039 0.0046 0.0073 0.0175
Bud 0.0011 0.0002 0.0004 0.0006 0.0011 0.0004 0.0008 0.0005 0.001 0.0011 0.0004 0.0007 0.0016 0.0039 0.0007 0.0006 0.0002 0.0005
Bud Neck 0.0074 0.0006 0.001 0.0022 0.002 0.0095 0.0012 0.0013 0.0009 0.0025 0.0019 0.0045 0.0066 0.0007 0.001 0.0036 0.0042 0.0038
Bud Periphery 0.0012 0.0001 0.0003 0.0007 0.0006 0.0003 0.0007 0.0001 0.0013 0.0012 0.0004 0.0005 0.0017 0.0017 0.0005 0.0008 0.0002 0.0004
Bud Site 0.0046 0.0008 0.0012 0.0012 0.0022 0.0005 0.0034 0.0065 0.0054 0.0049 0.0007 0.0008 0.0195 0.0032 0.0013 0.0025 0.0013 0.0007
Cell Periphery 0.0004 0 0.0001 0.0002 0.0001 0.0001 0.0002 0.0001 0.0002 0.0006 0.0001 0.0001 0.0006 0.0001 0.0011 0.0002 0.0001 0.0001
Cytoplasm 0.5677 0.76 0.6339 0.5132 0.7101 0.6621 0.5327 0.698 0.4753 0.4012 0.6185 0.5988 0.5856 0.7419 0.5622 0.4631 0.5918 0.6221
Cytoplasmic Foci 0.0254 0.0031 0.0066 0.0205 0.0375 0.0112 0.0408 0.0044 0.0159 0.0077 0.0235 0.0114 0.0283 0.0047 0.0053 0.0098 0.0245 0.0088
Eisosomes 0.0002 0 0.0002 0.0001 0 0 0.0002 0 0.0001 0.0001 0 0.0001 0.0008 0 0.0048 0.0001 0.0002 0.0001
Endoplasmic Reticulum 0.0104 0.0022 0.0045 0.0072 0.003 0.0065 0.0176 0.0027 0.0041 0.0053 0.0181 0.0041 0.0079 0.0035 0.0044 0.0015 0.0038 0.0037
Endosome 0.0467 0.0038 0.0102 0.0464 0.0268 0.0208 0.0264 0.0029 0.0146 0.0533 0.0826 0.011 0.0294 0.0024 0.0099 0.0046 0.0109 0.0052
Golgi 0.0122 0.0003 0.0036 0.0231 0.0105 0.0026 0.0099 0.0002 0.005 0.0151 0.0097 0.0082 0.0122 0.0002 0.0012 0.0034 0.0071 0.0045
Lipid Particles 0.0241 0 0.0024 0.0068 0.0005 0.0032 0.0072 0.0001 0.0029 0.0019 0.0111 0.0056 0.007 0.0001 0.0015 0.0012 0.0386 0.0066
Mitochondria 0.0201 0.0004 0.0157 0.0107 0.0303 0.0021 0.0172 0.0004 0.0027 0.0045 0.0053 0.0079 0.0129 0.0004 0.0047 0.0104 0.0073 0.0019
None 0.01 0.0036 0.0093 0.01 0.0093 0.0049 0.0437 0.0194 0.0187 0.0053 0.0096 0.0294 0.0135 0.0113 0.0137 0.0028 0.0074 0.0134
Nuclear Periphery 0.0226 0.0061 0.0163 0.0122 0.0112 0.0119 0.0171 0.0059 0.0106 0.0104 0.0181 0.0065 0.0127 0.0087 0.0117 0.0068 0.0086 0.009
Nucleolus 0.0032 0.0004 0.0006 0.0008 0.0003 0.0007 0.0007 0.001 0.0012 0.0008 0.0005 0.0017 0.0011 0.0005 0.0008 0.0006 0.0019 0.0008
Nucleus 0.1793 0.2132 0.2655 0.2701 0.1384 0.2453 0.2339 0.2357 0.381 0.4599 0.1667 0.2925 0.1654 0.1943 0.3519 0.4246 0.237 0.2791
Peroxisomes 0.0076 0.0001 0.0019 0.0061 0.0055 0.0024 0.007 0.0002 0.0025 0.0005 0.0019 0.0038 0.0142 0.0004 0.0003 0.0369 0.0277 0.0055
Punctate Nuclear 0.0163 0.003 0.0101 0.0345 0.0059 0.0069 0.0169 0.0184 0.0379 0.0071 0.0216 0.0072 0.0197 0.0183 0.0169 0.021 0.0173 0.015
Vacuole 0.0072 0.0013 0.0017 0.0029 0.0023 0.0033 0.0028 0.0015 0.0023 0.0071 0.0059 0.0022 0.0038 0.0009 0.0018 0.0009 0.0021 0.0012
Vacuole Periphery 0.0038 0.0002 0.0005 0.0018 0.001 0.0008 0.0009 0.0002 0.0003 0.0028 0.0015 0.0004 0.0012 0.0002 0.0004 0.0002 0.0004 0.0003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.5943 12.8276 6.0118 13.9096 12.7296 9.0579 15.7005 17.5077 16.5943 17.5465
Translational Efficiency 1.1203 1.1778 2.6113 1.0483 1.4622 2.3873 1.1572 1.1512 1.1005 1.0264

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘