Standard name
Human Ortholog
Description Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.76 0.65 0.75 0.68 0.63 0.82 0.76 0.84 0.71 0.76 0.6 0.61 0.96 0.97 0.96 0.61 0.66 0.63 0.6 0.52 0.76 0.76 0.69 0.52
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.09 0.21 0.32 0.18 0.4 0.37 0.52 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.07 0.08 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.27 0.27 0.09 0.13 0.14 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Vac/Vac Membrane 0.28 0.33 0.29 0.35 0.33 0.1 0.08 0.06 0.1 0.07 0.12 0.07 0.08 0 0.06 0.43 0.44 0.41 0.05 0.11 0.05 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2
Bud 1 0 0 2 3 1 3 1 12 2 2 6 0 0 0 0 0 1 5 4 0 2 4 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 4
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 208 39 89 84 96 155 228 264 173 211 94 129 223 366 413 90 116 114 176 47 96 137 267 218
Endoplasmic Reticulum 2 0 1 0 0 0 0 0 0 0 0 0 0 4 0 1 5 5 0 0 1 0 4 1
Endosome 1 0 1 4 6 3 0 5 1 0 3 0 4 1 4 6 4 10 3 1 0 0 8 47
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 2 0 2 0 0 0 2 7
Mitochondria 10 2 3 9 14 40 96 57 96 102 82 102 0 0 0 5 2 4 0 0 1 0 3 9
Nucleus 20 5 2 2 13 3 7 7 2 4 5 6 2 2 1 3 5 2 79 24 11 23 55 45
Nuclear Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 3
Nucleolus 1 0 0 0 0 0 2 2 1 1 2 0 1 0 1 9 2 5 0 0 1 0 0 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 7
SpindlePole 1 0 0 0 0 0 2 4 0 0 0 1 0 0 0 5 3 3 4 0 0 2 11 36
Vac/Vac Membrane 77 20 34 43 51 19 23 20 24 20 19 14 19 7 26 63 77 73 14 9 5 9 16 15
Unique Cell Count 272 60 118 123 153 190 300 314 243 276 157 210 233 376 428 148 177 180 293 90 128 180 386 420
Labelled Cell Count 322 66 130 144 184 221 361 362 309 342 208 261 250 381 445 186 216 218 293 90 128 180 386 420


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.3 6.9 5.9 5.2 4.7 4.4 4.6 4.2 4.3 4.2 4.1 8.5 8.3 8.2 10.5 9.7 9.3 7.3 7.8 7.9
Std Deviation (1e-4) 0.7 1.1 1.5 1.0 1.3 1.5 1.3 1.8 0.9 1.9 1.9 1.1 1.2 1.1 1.2 1.9 1.8 2.0 1.2 1.3 1.1
Intensity Change (Log2) -0.24 -0.42 -0.57 -0.65 -0.58 -0.72 -0.68 -0.72 -0.78 0.29 0.26 0.23 0.59 0.48 0.42 0.07 0.17 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.2 -2.2 1.3 0.1 2.1 -0.8 0.2 -2.7 -2.6 5.7 7.7 7.4 -2.5 -1.8 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.7 2.2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 2.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 0.8 -4.3 -5.7 -6.3 -4.6 -5.7 -3.5 -5.4 -5.1 -9.3 -7.0 2.3 2.6 2.1

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4656 4.6207 4.2536 3.9514 3.6367 4.2813 2.4479 4.4444 4.074 3.4025 3.2346 3.8589 2.5005 4.4452 3.4623 3.3096 3.6639 3.6769
Actin 0.0158 0.0017 0.006 0.0008 0.0086 0.0004 0.0149 0.0026 0.0206 0.0023 0.0058 0.0029 0.03 0.0004 0.0122 0.001 0.0053 0.0027
Bud 0.0047 0.0167 0.0003 0.0001 0.0005 0.0001 0.0014 0.0002 0.0003 0.0081 0.0009 0.0004 0.001 0.0038 0.0021 0.0012 0.0025 0.0016
Bud Neck 0.0036 0.0004 0.0007 0.0008 0.0017 0.002 0.0008 0.0003 0.0008 0.0009 0.0335 0.0019 0.0018 0.0004 0.0036 0.0014 0.005 0.0016
Bud Periphery 0.0015 0.0056 0.0002 0 0.0007 0 0.0014 0.0001 0.0002 0.0034 0.0012 0.0003 0.0008 0.0005 0.0008 0.0005 0.0012 0.0009
Bud Site 0.0056 0.0021 0.0026 0.0002 0.0013 0.0001 0.0083 0.0007 0.003 0.0005 0.0085 0.0005 0.0045 0.0022 0.0028 0.0003 0.0007 0.0002
Cell Periphery 0.0003 0.0001 0.0001 0 0.0001 0 0.0003 0 0 0.0001 0.0003 0 0.0003 0.0001 0.0002 0 0.0001 0.0001
Cytoplasm 0.7276 0.7306 0.7318 0.7429 0.6861 0.7375 0.7246 0.8585 0.7107 0.77 0.7009 0.7206 0.7168 0.804 0.6711 0.7955 0.7192 0.698
Cytoplasmic Foci 0.0111 0.0054 0.0068 0.002 0.0109 0.0093 0.0298 0.0027 0.0081 0.0121 0.0261 0.0085 0.0261 0.0137 0.0177 0.0056 0.0188 0.0133
Eisosomes 0.0002 0 0.0001 0 0.0001 0 0.0002 0 0.0001 0 0.0001 0 0.0005 0 0.0001 0 0 0
Endoplasmic Reticulum 0.0067 0.0044 0.0073 0.0029 0.0048 0.0039 0.0103 0.0015 0.0025 0.0026 0.0034 0.0026 0.0062 0.0014 0.0027 0.0027 0.0031 0.0023
Endosome 0.0113 0.0052 0.0056 0.0011 0.0149 0.0037 0.0336 0.0023 0.0116 0.0104 0.052 0.0119 0.0269 0.0005 0.0196 0.0027 0.0022 0.0094
Golgi 0.0036 0.0003 0.0007 0.0001 0.0042 0.0001 0.0086 0.0004 0.0059 0.0218 0.0426 0.0032 0.0083 0.0001 0.0066 0.0002 0.0003 0.0041
Lipid Particles 0.0058 0.0002 0.0009 0 0.004 0.0001 0.0115 0.0003 0.0064 0.0006 0.0043 0.001 0.016 0.0001 0.0039 0.0001 0.0002 0.0032
Mitochondria 0.0033 0.0005 0.0005 0.0003 0.0021 0.0003 0.0218 0.0012 0.0124 0.0033 0.0188 0.0094 0.0075 0.0003 0.0038 0.0003 0.0005 0.005
None 0.078 0.0699 0.0634 0.0382 0.0057 0.026 0.023 0.0045 0.0057 0.0131 0.0035 0.0035 0.0263 0.006 0.0096 0.0182 0.0154 0.004
Nuclear Periphery 0.0135 0.0061 0.0094 0.0109 0.0143 0.0077 0.0113 0.0049 0.0128 0.0059 0.0085 0.0101 0.0153 0.0047 0.009 0.0081 0.0069 0.0103
Nucleolus 0.0007 0.0003 0.0003 0.0002 0.0004 0.0002 0.0009 0.0001 0.0003 0.0001 0.0004 0.0002 0.0006 0.0002 0.0052 0.0005 0.0003 0.0003
Nucleus 0.0903 0.1441 0.1521 0.1942 0.2282 0.2002 0.0737 0.1166 0.1837 0.1357 0.0762 0.209 0.0909 0.1543 0.2151 0.1394 0.2025 0.2253
Peroxisomes 0.0052 0.0002 0.0006 0 0.0011 0.0001 0.0101 0.0003 0.0101 0.0049 0.0042 0.0049 0.0075 0.0001 0.0026 0.0002 0.0003 0.0009
Punctate Nuclear 0.0075 0.0034 0.0088 0.0045 0.0068 0.0067 0.0071 0.002 0.003 0.0024 0.0027 0.0072 0.008 0.0063 0.0086 0.0207 0.0138 0.0147
Vacuole 0.0029 0.0025 0.0016 0.0006 0.0028 0.0014 0.0048 0.0007 0.0011 0.0014 0.0029 0.0013 0.0034 0.0008 0.0021 0.0011 0.0014 0.0014
Vacuole Periphery 0.0007 0.0002 0.0003 0.0001 0.0009 0.0002 0.0017 0.0002 0.0006 0.0003 0.0031 0.0005 0.0013 0.0002 0.0007 0.0002 0.0004 0.0007

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 69.2896 84.925 74.5809 85.5456 96.6172 67.0735 80.5501 79.2226 96.8617 82.2016
Translational Efficiency 2.23 1.7873 1.7513 1.8701 1.5013 2.26 1.6332 1.6411 1.4478 1.5616

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p
Localization
Cell Percentages cytoplasm (66%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-9

Map2

Map2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Map2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available