Standard name
Description Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.76 0.65 0.75 0.68 0.63 0.82 0.76 0.84 0.71 0.76 0.6 0.61 0.96 0.97 0.96 0.61 0.66 0.63 0.6 0.52 0.76 0.76 0.69 0.52
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0.11
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.07 0.09 0.21 0.32 0.18 0.4 0.37 0.52 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.07 0.08 0 0 0.08 0 0 0 0 0 0 0 0 0 0 0 0 0 0.27 0.27 0.09 0.13 0.14 0.11
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Vac/Vac Membrane 0.28 0.33 0.29 0.35 0.33 0.1 0.08 0.06 0.1 0.07 0.12 0.07 0.08 0 0.06 0.43 0.44 0.41 0.05 0.11 0.05 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 2
Bud 1 0 0 2 3 1 3 1 12 2 2 6 0 0 0 0 0 1 5 4 0 2 4 14
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 3 4
Bud Site 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 1 0 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 208 39 89 84 96 155 228 264 173 211 94 129 223 366 413 90 116 114 176 47 96 137 267 218
Endoplasmic Reticulum 2 0 1 0 0 0 0 0 0 0 0 0 0 4 0 1 5 5 0 0 1 0 4 1
Endosome 1 0 1 4 6 3 0 5 1 0 3 0 4 1 4 6 4 10 3 1 0 0 8 47
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 1 1 0 2 2 0 2 0 0 0 2 7
Mitochondria 10 2 3 9 14 40 96 57 96 102 82 102 0 0 0 5 2 4 0 0 1 0 3 9
Nucleus 20 5 2 2 13 3 7 7 2 4 5 6 2 2 1 3 5 2 79 24 11 23 55 45
Nuclear Periphery 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 1 0 0 0 0 3
Nucleolus 1 0 0 0 0 0 2 2 1 1 2 0 1 0 1 9 2 5 0 0 1 0 0 1
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 7
SpindlePole 1 0 0 0 0 0 2 4 0 0 0 1 0 0 0 5 3 3 4 0 0 2 11 36
Vac/Vac Membrane 77 20 34 43 51 19 23 20 24 20 19 14 19 7 26 63 77 73 14 9 5 9 16 15
Unique Cell Count 272 60 118 123 153 190 300 314 243 276 157 210 233 376 428 148 177 180 293 90 128 180 386 420
Labelled Cell Count 322 66 130 144 184 221 361 362 309 342 208 261 250 381 445 186 216 218 293 90 128 180 386 420


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.7 6.3 6.9 5.9 5.2 4.7 4.4 4.6 4.2 4.3 4.2 4.1 8.5 8.3 8.2 10.5 9.7 9.3 7.3 7.8 7.9
Std Deviation (1e-4) 0.7 1.1 1.5 1.0 1.3 1.5 1.3 1.8 0.9 1.9 1.9 1.1 1.2 1.1 1.2 1.9 1.8 2.0 1.2 1.3 1.1
Intensity Change (Log2) -0.24 -0.42 -0.57 -0.65 -0.58 -0.72 -0.68 -0.72 -0.78 0.29 0.26 0.23 0.59 0.48 0.42 0.07 0.17 0.19


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.2 -2.2 1.3 0.1 2.1 -0.8 0.2 -2.7 -2.6 5.7 7.7 7.4 -2.5 -1.8 -2.2
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 1.7 2.2 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 2.4 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 1.0 0.8 -4.3 -5.7 -6.3 -4.6 -5.7 -3.5 -5.4 -5.1 -9.3 -7.0 2.3 2.6 2.1


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Map2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available