Standard name | |
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Human Ortholog | |
Description | Methionine aminopeptidase; catalyzes the cotranslational removal of N-terminal methionine from nascent polypeptides; function is partially redundant with that of Map1p |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 2 |
Bud | 1 | 0 | 0 | 2 | 3 | 1 | 3 | 1 | 12 | 2 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 1 | 5 | 4 | 0 | 2 | 4 | 14 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 3 | 4 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 2 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 208 | 39 | 89 | 84 | 96 | 155 | 228 | 264 | 173 | 211 | 94 | 129 | 223 | 366 | 413 | 90 | 116 | 114 | 176 | 47 | 96 | 137 | 267 | 218 |
Endoplasmic Reticulum | 2 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 0 | 1 | 5 | 5 | 0 | 0 | 1 | 0 | 4 | 1 |
Endosome | 1 | 0 | 1 | 4 | 6 | 3 | 0 | 5 | 1 | 0 | 3 | 0 | 4 | 1 | 4 | 6 | 4 | 10 | 3 | 1 | 0 | 0 | 8 | 47 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 2 | 0 | 2 | 0 | 0 | 0 | 2 | 7 |
Mitochondria | 10 | 2 | 3 | 9 | 14 | 40 | 96 | 57 | 96 | 102 | 82 | 102 | 0 | 0 | 0 | 5 | 2 | 4 | 0 | 0 | 1 | 0 | 3 | 9 |
Nucleus | 20 | 5 | 2 | 2 | 13 | 3 | 7 | 7 | 2 | 4 | 5 | 6 | 2 | 2 | 1 | 3 | 5 | 2 | 79 | 24 | 11 | 23 | 55 | 45 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 3 |
Nucleolus | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 9 | 2 | 5 | 0 | 0 | 1 | 0 | 0 | 1 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 7 |
SpindlePole | 1 | 0 | 0 | 0 | 0 | 0 | 2 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 3 | 3 | 4 | 0 | 0 | 2 | 11 | 36 |
Vac/Vac Membrane | 77 | 20 | 34 | 43 | 51 | 19 | 23 | 20 | 24 | 20 | 19 | 14 | 19 | 7 | 26 | 63 | 77 | 73 | 14 | 9 | 5 | 9 | 16 | 15 |
Unique Cell Count | 272 | 60 | 118 | 123 | 153 | 190 | 300 | 314 | 243 | 276 | 157 | 210 | 233 | 376 | 428 | 148 | 177 | 180 | 293 | 90 | 128 | 180 | 386 | 420 |
Labelled Cell Count | 322 | 66 | 130 | 144 | 184 | 221 | 361 | 362 | 309 | 342 | 208 | 261 | 250 | 381 | 445 | 186 | 216 | 218 | 293 | 90 | 128 | 180 | 386 | 420 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.7 | 6.3 | 6.9 | 5.9 | 5.2 | 4.7 | 4.4 | 4.6 | 4.2 | 4.3 | 4.2 | 4.1 | 8.5 | 8.3 | 8.2 | 10.5 | 9.7 | 9.3 | 7.3 | 7.8 | 7.9 |
Std Deviation (1e-4) | 0.7 | 1.1 | 1.5 | 1.0 | 1.3 | 1.5 | 1.3 | 1.8 | 0.9 | 1.9 | 1.9 | 1.1 | 1.2 | 1.1 | 1.2 | 1.9 | 1.8 | 2.0 | 1.2 | 1.3 | 1.1 |
Intensity Change (Log2) | – | – | – | -0.24 | -0.42 | -0.57 | -0.65 | -0.58 | -0.72 | -0.68 | -0.72 | -0.78 | 0.29 | 0.26 | 0.23 | 0.59 | 0.48 | 0.42 | 0.07 | 0.17 | 0.19 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.2 | -2.2 | 1.3 | 0.1 | 2.1 | -0.8 | 0.2 | -2.7 | -2.6 | 5.7 | 7.7 | 7.4 | -2.5 | -1.8 | -2.2 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 1.7 | 2.2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 2.4 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 1.0 | 0.8 | -4.3 | -5.7 | -6.3 | -4.6 | -5.7 | -3.5 | -5.4 | -5.1 | -9.3 | -7.0 | 2.3 | 2.6 | 2.1 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 3.4656 | 4.6207 | 4.2536 | 3.9514 | 3.6367 | 4.2813 | 2.4479 | 4.4444 | 4.074 | 3.4025 | 3.2346 | 3.8589 | 2.5005 | 4.4452 | 3.4623 | 3.3096 | 3.6639 | 3.6769 |
Actin | 0.0158 | 0.0017 | 0.006 | 0.0008 | 0.0086 | 0.0004 | 0.0149 | 0.0026 | 0.0206 | 0.0023 | 0.0058 | 0.0029 | 0.03 | 0.0004 | 0.0122 | 0.001 | 0.0053 | 0.0027 |
Bud | 0.0047 | 0.0167 | 0.0003 | 0.0001 | 0.0005 | 0.0001 | 0.0014 | 0.0002 | 0.0003 | 0.0081 | 0.0009 | 0.0004 | 0.001 | 0.0038 | 0.0021 | 0.0012 | 0.0025 | 0.0016 |
Bud Neck | 0.0036 | 0.0004 | 0.0007 | 0.0008 | 0.0017 | 0.002 | 0.0008 | 0.0003 | 0.0008 | 0.0009 | 0.0335 | 0.0019 | 0.0018 | 0.0004 | 0.0036 | 0.0014 | 0.005 | 0.0016 |
Bud Periphery | 0.0015 | 0.0056 | 0.0002 | 0 | 0.0007 | 0 | 0.0014 | 0.0001 | 0.0002 | 0.0034 | 0.0012 | 0.0003 | 0.0008 | 0.0005 | 0.0008 | 0.0005 | 0.0012 | 0.0009 |
Bud Site | 0.0056 | 0.0021 | 0.0026 | 0.0002 | 0.0013 | 0.0001 | 0.0083 | 0.0007 | 0.003 | 0.0005 | 0.0085 | 0.0005 | 0.0045 | 0.0022 | 0.0028 | 0.0003 | 0.0007 | 0.0002 |
Cell Periphery | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0003 | 0 | 0 | 0.0001 | 0.0003 | 0 | 0.0003 | 0.0001 | 0.0002 | 0 | 0.0001 | 0.0001 |
Cytoplasm | 0.7276 | 0.7306 | 0.7318 | 0.7429 | 0.6861 | 0.7375 | 0.7246 | 0.8585 | 0.7107 | 0.77 | 0.7009 | 0.7206 | 0.7168 | 0.804 | 0.6711 | 0.7955 | 0.7192 | 0.698 |
Cytoplasmic Foci | 0.0111 | 0.0054 | 0.0068 | 0.002 | 0.0109 | 0.0093 | 0.0298 | 0.0027 | 0.0081 | 0.0121 | 0.0261 | 0.0085 | 0.0261 | 0.0137 | 0.0177 | 0.0056 | 0.0188 | 0.0133 |
Eisosomes | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0005 | 0 | 0.0001 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0067 | 0.0044 | 0.0073 | 0.0029 | 0.0048 | 0.0039 | 0.0103 | 0.0015 | 0.0025 | 0.0026 | 0.0034 | 0.0026 | 0.0062 | 0.0014 | 0.0027 | 0.0027 | 0.0031 | 0.0023 |
Endosome | 0.0113 | 0.0052 | 0.0056 | 0.0011 | 0.0149 | 0.0037 | 0.0336 | 0.0023 | 0.0116 | 0.0104 | 0.052 | 0.0119 | 0.0269 | 0.0005 | 0.0196 | 0.0027 | 0.0022 | 0.0094 |
Golgi | 0.0036 | 0.0003 | 0.0007 | 0.0001 | 0.0042 | 0.0001 | 0.0086 | 0.0004 | 0.0059 | 0.0218 | 0.0426 | 0.0032 | 0.0083 | 0.0001 | 0.0066 | 0.0002 | 0.0003 | 0.0041 |
Lipid Particles | 0.0058 | 0.0002 | 0.0009 | 0 | 0.004 | 0.0001 | 0.0115 | 0.0003 | 0.0064 | 0.0006 | 0.0043 | 0.001 | 0.016 | 0.0001 | 0.0039 | 0.0001 | 0.0002 | 0.0032 |
Mitochondria | 0.0033 | 0.0005 | 0.0005 | 0.0003 | 0.0021 | 0.0003 | 0.0218 | 0.0012 | 0.0124 | 0.0033 | 0.0188 | 0.0094 | 0.0075 | 0.0003 | 0.0038 | 0.0003 | 0.0005 | 0.005 |
None | 0.078 | 0.0699 | 0.0634 | 0.0382 | 0.0057 | 0.026 | 0.023 | 0.0045 | 0.0057 | 0.0131 | 0.0035 | 0.0035 | 0.0263 | 0.006 | 0.0096 | 0.0182 | 0.0154 | 0.004 |
Nuclear Periphery | 0.0135 | 0.0061 | 0.0094 | 0.0109 | 0.0143 | 0.0077 | 0.0113 | 0.0049 | 0.0128 | 0.0059 | 0.0085 | 0.0101 | 0.0153 | 0.0047 | 0.009 | 0.0081 | 0.0069 | 0.0103 |
Nucleolus | 0.0007 | 0.0003 | 0.0003 | 0.0002 | 0.0004 | 0.0002 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0004 | 0.0002 | 0.0006 | 0.0002 | 0.0052 | 0.0005 | 0.0003 | 0.0003 |
Nucleus | 0.0903 | 0.1441 | 0.1521 | 0.1942 | 0.2282 | 0.2002 | 0.0737 | 0.1166 | 0.1837 | 0.1357 | 0.0762 | 0.209 | 0.0909 | 0.1543 | 0.2151 | 0.1394 | 0.2025 | 0.2253 |
Peroxisomes | 0.0052 | 0.0002 | 0.0006 | 0 | 0.0011 | 0.0001 | 0.0101 | 0.0003 | 0.0101 | 0.0049 | 0.0042 | 0.0049 | 0.0075 | 0.0001 | 0.0026 | 0.0002 | 0.0003 | 0.0009 |
Punctate Nuclear | 0.0075 | 0.0034 | 0.0088 | 0.0045 | 0.0068 | 0.0067 | 0.0071 | 0.002 | 0.003 | 0.0024 | 0.0027 | 0.0072 | 0.008 | 0.0063 | 0.0086 | 0.0207 | 0.0138 | 0.0147 |
Vacuole | 0.0029 | 0.0025 | 0.0016 | 0.0006 | 0.0028 | 0.0014 | 0.0048 | 0.0007 | 0.0011 | 0.0014 | 0.0029 | 0.0013 | 0.0034 | 0.0008 | 0.0021 | 0.0011 | 0.0014 | 0.0014 |
Vacuole Periphery | 0.0007 | 0.0002 | 0.0003 | 0.0001 | 0.0009 | 0.0002 | 0.0017 | 0.0002 | 0.0006 | 0.0003 | 0.0031 | 0.0005 | 0.0013 | 0.0002 | 0.0007 | 0.0002 | 0.0004 | 0.0007 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 69.2896 | 84.925 | 74.5809 | 85.5456 | 96.6172 | 67.0735 | 80.5501 | 79.2226 | 96.8617 | 82.2016 |
Translational Efficiency | 2.23 | 1.7873 | 1.7513 | 1.8701 | 1.5013 | 2.26 | 1.6332 | 1.6411 | 1.4478 | 1.5616 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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