Standard name
Description Ribosomal 60S subunit protein L23A; homologous to mammalian ribosomal protein L23 and bacterial L14; RPL23A has a paralog, RPL23B, that arose from the whole genome duplication

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.05 0 0 0 0.05 0.2 0.2 0.18 0.24 0.16 0.13 0.17 0.05 0.06 0.16 0 0.05 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0.11 0.07 0.06 0.06 0.33 0.23 0.29 0.38 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.94 0.95 0.94 0.94 0.87 0.64 0.71 0.73 0.55 0.65 0.64 0.59 0.93 0.9 0.78 0.8 0.81 0.76 0.94 0.94 0.94 0.68 0.59 0.46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0.06 0.06 0 0 0 0.13 0.09 0.11 0 0 0 0.07 0.12 0.1
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09
Vac/Vac Membrane 0 0 0 0 0 0.1 0 0 0 0 0.09 0 0 0 0.06 0 0 0.08 0 0 0 0.19 0.19 0.26
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 1 4 5 1 1 0 0 0 0 0 0 5 2 0 3 5 4
Bud Neck 0 0 0 0 0 1 0 0 0 2 1 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 1 0 1 1 6 8 2 8 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0
Cytoplasm 19 12 12 9 10 58 56 55 70 56 34 35 18 23 78 14 15 15 1 3 0 1 3 0
Endoplasmic Reticulum 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 1 1 0 0 0 0 0 0
Endosome 1 1 0 0 0 6 3 3 2 2 3 0 1 4 7 11 10 5 0 1 0 1 5 9
Golgi 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 0 1 0 0 0 0 1 2
Mitochondria 0 1 0 0 20 20 18 17 96 78 78 80 0 0 0 7 4 1 0 2 0 1 3 5
Nucleus 344 528 257 198 164 185 204 221 162 224 171 124 347 326 380 294 269 201 363 549 269 224 206 174
Nuclear Periphery 0 2 1 1 0 1 2 0 1 4 2 2 0 0 1 2 0 0 0 0 0 1 1 2
Nucleolus 5 7 4 2 5 2 2 7 13 11 16 12 3 3 4 46 30 28 5 1 4 24 41 38
Peroxisomes 0 0 0 0 0 1 0 0 1 0 0 0 0 0 2 4 3 4 0 0 0 0 1 2
SpindlePole 0 0 0 0 0 3 3 4 2 1 2 3 0 2 3 12 10 10 0 0 0 3 7 32
Vac/Vac Membrane 9 11 7 2 7 29 11 13 10 10 25 8 4 11 27 10 11 20 8 15 5 63 66 96
Unique Cell Count 367 555 274 211 189 287 286 301 293 345 267 211 372 363 490 366 331 263 387 584 285 331 349 378
Labelled Cell Count 378 562 281 212 209 306 300 322 367 401 335 273 374 369 502 402 354 286 387 584 285 331 349 378


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 27.5 21.1 25.8 18.2 5.7 5.4 5.2 5.7 4.4 4.9 5.2 4.6 25.5 16.1 10.6 16.5 24.3 22.6 23.6 18.7 17.1
Std Deviation (1e-4) 12.5 7.0 10.2 6.8 1.7 1.9 1.5 1.6 1.9 1.9 2.0 1.9 8.0 5.1 3.3 5.7 9.0 8.3 8.9 6.9 5.2
Intensity Change (Log2) -0.51 -2.18 -2.25 -2.31 -2.17 -2.55 -2.39 -2.32 -2.49 -0.02 -0.68 -1.29 -0.64 -0.09 -0.2 -0.13 -0.46 -0.6


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -0.1 0.5 5.7 5.5 5.2 6.6 4.7 3.5 4.5 0.3 1.1 4.7 -0.4 0.1 0.7
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 5.5 4.4 4.2 4.0 0 8.4 9.7 0 0 0 0 0 0 0
Nucleus 0 -2.6 -8.5 -7.0 -6.5 -10.4 -8.6 -8.5 -9.3 -0.3 -1.8 -5.8 -4.9 -4.6 -5.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0.8 0 1.4 2.8 0 0 0 0 5.2 4.0 4.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 3.0 0 0
Vacuole 0 0.7 3.6 0.9 1.2 0.6 0.3 3.4 0.8 0 0.4 1.9 0.1 0.6 2.7