Standard name | |
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Human Ortholog | |
Description | Protein that associates with mitochondrial ribosome; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 15 | 15 | 0 | 4 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 10 | 2 | 1 | – | – | – | 8 | 17 | 5 | 6 | 26 | 16 |
Bud | 2 | 3 | 11 | 4 | 2 | 2 | 5 | 11 | 8 | 16 | 3 | 19 | 2 | 3 | 0 | – | – | – | 2 | 4 | 8 | 2 | 20 | 25 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 1 | 2 | 0 | 3 | 11 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 9 | 20 | 6 | 4 | 6 | 3 | 6 | 8 | 4 | 3 | 2 | 1 | 9 | 11 | 9 | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 15 | 27 | 233 | 126 | 249 | 275 | 348 | 319 | 149 | 220 | 113 | 211 | 176 | 316 | 262 | – | – | – | 1 | 4 | 188 | 68 | 121 | 116 |
Endoplasmic Reticulum | 5 | 14 | 17 | 1 | 3 | 8 | 2 | 5 | 9 | 3 | 5 | 4 | 10 | 8 | 12 | – | – | – | 3 | 4 | 5 | 3 | 6 | 6 |
Endosome | 34 | 8 | 8 | 2 | 17 | 9 | 12 | 18 | 2 | 2 | 0 | 0 | 16 | 31 | 15 | – | – | – | 70 | 38 | 19 | 15 | 42 | 46 |
Golgi | 13 | 57 | 18 | 9 | 9 | 1 | 1 | 3 | 0 | 1 | 1 | 0 | 21 | 30 | 21 | – | – | – | 47 | 45 | 20 | 20 | 36 | 28 |
Mitochondria | 188 | 92 | 79 | 57 | 94 | 191 | 253 | 257 | 179 | 281 | 152 | 338 | 33 | 50 | 19 | – | – | – | 141 | 118 | 81 | 38 | 104 | 72 |
Nucleus | 2 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 2 | 2 | 3 | 3 | 1 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 1 | 6 | 4 | 4 | 5 | 5 | 5 | 9 | 0 | 0 | 0 | – | – | – | 7 | 2 | 2 | 1 | 5 | 3 |
Nucleolus | 19 | 2 | 0 | 0 | 2 | 4 | 5 | 8 | 3 | 7 | 4 | 4 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 2 | 2 |
Peroxisomes | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 6 | 1 | – | – | – | 6 | 5 | 7 | 4 | 6 | 9 |
SpindlePole | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 3 | 3 | 1 | 3 | 5 | 0 | 2 | 0 | – | – | – | 6 | 3 | 7 | 4 | 12 | 12 |
Vac/Vac Membrane | 63 | 65 | 10 | 2 | 18 | 23 | 32 | 27 | 9 | 14 | 5 | 10 | 18 | 39 | 34 | – | – | – | 12 | 31 | 3 | 2 | 9 | 9 |
Unique Cell Count | 276 | 228 | 344 | 180 | 360 | 437 | 578 | 557 | 289 | 437 | 229 | 471 | 264 | 457 | 352 | 320 | 288 | 359 | 173 | 406 | 372 | |||
Labelled Cell Count | 358 | 309 | 400 | 206 | 407 | 526 | 673 | 669 | 374 | 558 | 297 | 606 | 296 | 498 | 374 | 320 | 288 | 359 | 173 | 406 | 372 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.1 | 9.3 | 5.7 | 5.5 | 5.4 | 4.5 | 4.5 | 4.6 | 4.2 | 3.9 | 3.9 | 3.8 | 7.8 | 7.7 | 7.5 | – | – | – | 7.2 | 7.4 | 7.9 |
Std Deviation (1e-4) | 1.6 | 1.6 | 1.6 | 2.0 | 1.9 | 1.7 | 1.7 | 1.6 | 1.5 | 1.4 | 1.4 | 1.3 | 2.5 | 2.2 | 2.2 | – | – | – | 2.3 | 2.6 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.07 | -0.33 | -0.36 | -0.3 | -0.43 | -0.56 | -0.55 | -0.57 | 0.45 | 0.43 | 0.39 | – | – | – | 0.33 | 0.37 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.8 | -2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | -3.8 | -3.6 | – | – | – |
Bud | -0.6 | -2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | -2.7 | -3.4 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0.6 | 0.7 | – | – | – |
Cytoplasm | 0.5 | 0.4 | -1.4 | -2.3 | -3.1 | -4.1 | -4.9 | -4.4 | -6.5 | -0.3 | 0.4 | 1.9 | – | – | – |
Endoplasmic Reticulum | -2.6 | -3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.7 | -2.6 | -1.0 | – | – | – |
Endosome | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.9 | 1.4 | – | – | – |
Golgi | -0.1 | -1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0.8 | 0.4 | – | – | – |
Mitochondria | 2.2 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.3 | -4.6 | -6.7 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 1.4 | 1.6 | 1.9 | 1.4 | 0.2 | 0.2 | -0.5 | -0.7 | 2.3 | 3.3 | 3.7 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 5.0735 | 6.4505 | 6.0751 | 5.9635 | 5.5417 | 6.0617 | 6.8222 | 7.3944 | 7.7226 | 6.5769 | 7.0518 | 7.5384 | 8.3708 | 9.6822 | 9.6348 | 9.5814 | 8.6258 | 9.4223 |
Actin | 0.0344 | 0.061 | 0.0425 | 0.0692 | 0.0053 | 0.0116 | 0.0396 | 0.0423 | 0.0356 | 0.0252 | 0.0164 | 0.0209 | 0.0793 | 0.0725 | 0.0501 | 0.0549 | 0.0831 | 0.0559 |
Bud | 0.005 | 0.009 | 0.003 | 0.0104 | 0.0151 | 0.0017 | 0.0017 | 0.0038 | 0.0017 | 0.0021 | 0.0017 | 0.0006 | 0.0033 | 0.0065 | 0.0026 | 0.0096 | 0.0028 | 0.0012 |
Bud Neck | 0.0086 | 0.0031 | 0.0075 | 0.0011 | 0.0008 | 0.0085 | 0.0037 | 0.0008 | 0.0006 | 0.0004 | 0.0004 | 0.0015 | 0.0044 | 0.0049 | 0.0015 | 0.0015 | 0.001 | 0.0016 |
Bud Periphery | 0.0074 | 0.0088 | 0.0049 | 0.0192 | 0.0267 | 0.0043 | 0.0024 | 0.0027 | 0.0015 | 0.0035 | 0.0065 | 0.0011 | 0.0058 | 0.0085 | 0.0029 | 0.0238 | 0.0043 | 0.0023 |
Bud Site | 0.0095 | 0.0709 | 0.0125 | 0.0019 | 0.0102 | 0.0012 | 0.0107 | 0.0182 | 0.01 | 0.0016 | 0.0019 | 0.0013 | 0.017 | 0.0356 | 0.0134 | 0.0041 | 0.0034 | 0.0025 |
Cell Periphery | 0.0009 | 0.003 | 0.0015 | 0.0006 | 0.0017 | 0.0006 | 0.0009 | 0.0003 | 0.0006 | 0.0002 | 0.0013 | 0.0011 | 0.0035 | 0.0015 | 0.0005 | 0.0051 | 0.0003 | 0.0009 |
Cytoplasm | 0.003 | 0.0165 | 0.0084 | 0.0023 | 0.0067 | 0.0016 | 0.0281 | 0.0263 | 0.014 | 0.0031 | 0.0077 | 0.0035 | 0.0052 | 0.0251 | 0.026 | 0.0223 | 0.0036 | 0.0043 |
Cytoplasmic Foci | 0.0202 | 0.061 | 0.0277 | 0.0232 | 0.0133 | 0.0202 | 0.0423 | 0.0656 | 0.0466 | 0.0368 | 0.0593 | 0.0309 | 0.0384 | 0.0674 | 0.0391 | 0.0289 | 0.0379 | 0.0227 |
Eisosomes | 0.0008 | 0.0007 | 0.0008 | 0.0007 | 0.0002 | 0.0003 | 0.0008 | 0.0003 | 0.0006 | 0.0005 | 0.001 | 0.0004 | 0.0024 | 0.0008 | 0.0004 | 0.0008 | 0.0003 | 0.0018 |
Endoplasmic Reticulum | 0.0057 | 0.0011 | 0.003 | 0.0014 | 0.0008 | 0.0031 | 0.003 | 0.0014 | 0.0008 | 0.0003 | 0.006 | 0.0014 | 0.0065 | 0.0033 | 0.009 | 0.0226 | 0.0012 | 0.0041 |
Endosome | 0.0814 | 0.1335 | 0.0779 | 0.0348 | 0.0676 | 0.0813 | 0.0989 | 0.1229 | 0.0821 | 0.0419 | 0.1316 | 0.0632 | 0.1206 | 0.1475 | 0.106 | 0.1453 | 0.0943 | 0.0774 |
Golgi | 0.0733 | 0.1122 | 0.1057 | 0.0813 | 0.1088 | 0.1211 | 0.154 | 0.2134 | 0.1518 | 0.1685 | 0.2152 | 0.1841 | 0.1663 | 0.2452 | 0.1579 | 0.1865 | 0.2297 | 0.2014 |
Lipid Particles | 0.0367 | 0.0079 | 0.007 | 0.0062 | 0.0065 | 0.0116 | 0.0298 | 0.0031 | 0.0052 | 0.0131 | 0.006 | 0.0076 | 0.0263 | 0.011 | 0.0099 | 0.0021 | 0.0332 | 0.0086 |
Mitochondria | 0.6085 | 0.465 | 0.6411 | 0.7227 | 0.7096 | 0.696 | 0.5141 | 0.4614 | 0.6211 | 0.6608 | 0.4957 | 0.6482 | 0.454 | 0.3326 | 0.5525 | 0.4721 | 0.4741 | 0.5874 |
None | 0.0149 | 0.0007 | 0.0005 | 0.0023 | 0.0003 | 0.0002 | 0.0098 | 0.0003 | 0.0004 | 0.0002 | 0.0011 | 0.0002 | 0.0034 | 0.0005 | 0.0013 | 0.0009 | 0.0003 | 0.0004 |
Nuclear Periphery | 0.0164 | 0.0005 | 0.0021 | 0.0005 | 0.0002 | 0.0022 | 0.005 | 0.0002 | 0.0002 | 0.0001 | 0.0011 | 0.0001 | 0.0046 | 0.0006 | 0.0038 | 0.0036 | 0.0007 | 0.0053 |
Nucleolus | 0.0069 | 0.0009 | 0.0006 | 0.0002 | 0.0022 | 0.0002 | 0.0024 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.004 | 0.0008 | 0.0002 | 0.0001 | 0.0001 | 0.0002 |
Nucleus | 0.0079 | 0.001 | 0.0014 | 0.0003 | 0.0007 | 0.0005 | 0.0041 | 0.0002 | 0.0002 | 0.0001 | 0.0004 | 0.0002 | 0.0019 | 0.0008 | 0.0008 | 0.0006 | 0.0002 | 0.0018 |
Peroxisomes | 0.0194 | 0.0247 | 0.0151 | 0.014 | 0.0083 | 0.0124 | 0.0196 | 0.0304 | 0.0229 | 0.0394 | 0.0407 | 0.0289 | 0.0227 | 0.0181 | 0.0078 | 0.0042 | 0.0242 | 0.01 |
Punctate Nuclear | 0.0076 | 0.0005 | 0.0007 | 0.0006 | 0.0001 | 0.0001 | 0.0162 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.005 | 0.0005 | 0.0016 | 0.0002 | 0.0004 | 0.0004 |
Vacuole | 0.0149 | 0.0123 | 0.0232 | 0.0037 | 0.0106 | 0.0113 | 0.0084 | 0.0035 | 0.0017 | 0.0008 | 0.0025 | 0.0017 | 0.0125 | 0.0098 | 0.0071 | 0.0056 | 0.002 | 0.0038 |
Vacuole Periphery | 0.0168 | 0.0056 | 0.0129 | 0.0033 | 0.0043 | 0.0101 | 0.0046 | 0.0025 | 0.002 | 0.001 | 0.0031 | 0.0027 | 0.0127 | 0.0064 | 0.0055 | 0.0052 | 0.0029 | 0.0059 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 13.0852 | 18.9688 | 20.6443 | 21.7961 | 10.5462 | 11.3107 | 15.8229 | 18.9856 | 16.2169 | 15.1169 |
Translational Efficiency | 0.8978 | 1.0329 | 1.0291 | 0.915 | 1.1385 | 0.6305 | 1.0801 | 0.9472 | 1.1692 | 0.791 |
Dataset | Hit |
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Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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