Standard name
Description Protein that associates with mitochondrial ribosome; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0.06 0 0 0.06 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0.05 0.07
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0.09 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0.05 0.12 0.68 0.7 0.69 0.63 0.6 0.57 0.52 0.5 0.49 0.45 0.67 0.69 0.74 – – – 0 0 0.53 0.4 0.3 0.31
Endoplasmic Reticulum 0 0.06 0.05 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0.12 0 0 0 0.05 0 0 0 0 0 0 0 0.06 0.07 0 – – – 0.22 0.13 0.05 0.09 0.1 0.12
Golgi 0.05 0.25 0.05 0.05 0 0 0 0 0 0 0 0 0.08 0.07 0.06 – – – 0.15 0.16 0.06 0.12 0.09 0.08
Mitochondria 0.68 0.4 0.23 0.32 0.26 0.44 0.44 0.46 0.62 0.64 0.66 0.72 0.12 0.11 0.05 – – – 0.44 0.41 0.23 0.22 0.26 0.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.23 0.29 0 0 0.05 0.05 0.06 0.05 0 0 0 0 0.07 0.09 0.1 – – – 0 0.11 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 2 15 15 0 4 1 0 2 0 0 1 1 10 2 1 – – – 8 17 5 6 26 16
Bud 2 3 11 4 2 2 5 11 8 16 3 19 2 3 0 – – – 2 4 8 2 20 25
Bud Neck 0 0 0 0 0 0 2 0 0 2 0 0 0 0 0 – – – 3 1 2 0 3 11
Bud Site 0 0 0 0 0 1 1 2 0 1 0 1 0 0 0 – – – – – – – – –
Cell Periphery 9 20 6 4 6 3 6 8 4 3 2 1 9 11 9 – – – 0 0 1 0 1 0
Cytoplasm 15 27 233 126 249 275 348 319 149 220 113 211 176 316 262 – – – 1 4 188 68 121 116
Endoplasmic Reticulum 5 14 17 1 3 8 2 5 9 3 5 4 10 8 12 – – – 3 4 5 3 6 6
Endosome 34 8 8 2 17 9 12 18 2 2 0 0 16 31 15 – – – 70 38 19 15 42 46
Golgi 13 57 18 9 9 1 1 3 0 1 1 0 21 30 21 – – – 47 45 20 20 36 28
Mitochondria 188 92 79 57 94 191 253 257 179 281 152 338 33 50 19 – – – 141 118 81 38 104 72
Nucleus 2 2 1 0 1 1 1 1 2 2 3 3 1 0 0 – – – 1 0 0 0 0 1
Nuclear Periphery 1 0 1 0 1 6 4 4 5 5 5 9 0 0 0 – – – 7 2 2 1 5 3
Nucleolus 19 2 0 0 2 4 5 8 3 7 4 4 0 0 0 – – – 0 1 0 0 2 2
Peroxisomes 3 3 1 0 0 0 0 1 1 0 0 0 0 6 1 – – – 6 5 7 4 6 9
SpindlePole 2 1 0 1 1 1 1 3 3 1 3 5 0 2 0 – – – 6 3 7 4 12 12
Vac/Vac Membrane 63 65 10 2 18 23 32 27 9 14 5 10 18 39 34 – – – 12 31 3 2 9 9
Unique Cell Count 276 228 344 180 360 437 578 557 289 437 229 471 264 457 352 320 288 359 173 406 372
Labelled Cell Count 358 309 400 206 407 526 673 669 374 558 297 606 296 498 374 320 288 359 173 406 372


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.1 9.3 5.7 5.5 5.4 4.5 4.5 4.6 4.2 3.9 3.9 3.8 7.8 7.7 7.5 – – – 7.2 7.4 7.9
Std Deviation (1e-4) 1.6 1.6 1.6 2.0 1.9 1.7 1.7 1.6 1.5 1.4 1.4 1.3 2.5 2.2 2.2 – – – 2.3 2.6 2.2
Intensity Change (Log2) – – – -0.04 -0.07 -0.33 -0.36 -0.3 -0.43 -0.56 -0.55 -0.57 0.45 0.43 0.39 – – – 0.33 0.37 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.8 -2.7 0 0 0 0 0 0 0 -0.4 -3.8 -3.6 – – –
Bud -0.6 -2.6 0 0 0 0 0 0 0 -2.1 -2.7 -3.4 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 -0.1 0 0 0 0 0 0 0 1.3 0.6 0.7 – – –
Cytoplasm 0.5 0.4 -1.4 -2.3 -3.1 -4.1 -4.9 -4.4 -6.5 -0.3 0.4 1.9 – – –
Endoplasmic Reticulum -2.6 -3.3 0 0 0 0 0 0 0 -0.7 -2.6 -1.0 – – –
Endosome 0 1.7 0 0 0 0 0 0 0 2.3 2.9 1.4 – – –
Golgi -0.1 -1.9 0 0 0 0 0 0 0 1.4 0.8 0.4 – – –
Mitochondria 2.2 1.0 0 0 0 0 0 0 0 -3.3 -4.6 -6.7 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole 0 1.4 1.6 1.9 1.4 0.2 0.2 -0.5 -0.7 2.3 3.3 3.7 – – –


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Mrx12-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available