Standard name | |
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Description | Protein that associates with mitochondrial ribosome; detected in highly purified mitochondria in high-throughput studies; predicted to be involved in ribosome biogenesis |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 2 | 15 | 15 | 0 | 4 | 1 | 0 | 2 | 0 | 0 | 1 | 1 | 10 | 2 | 1 | – | – | – | 8 | 17 | 5 | 6 | 26 | 16 |
Bud | 2 | 3 | 11 | 4 | 2 | 2 | 5 | 11 | 8 | 16 | 3 | 19 | 2 | 3 | 0 | – | – | – | 2 | 4 | 8 | 2 | 20 | 25 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | – | – | 3 | 1 | 2 | 0 | 3 | 11 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 9 | 20 | 6 | 4 | 6 | 3 | 6 | 8 | 4 | 3 | 2 | 1 | 9 | 11 | 9 | – | – | – | 0 | 0 | 1 | 0 | 1 | 0 |
Cytoplasm | 15 | 27 | 233 | 126 | 249 | 275 | 348 | 319 | 149 | 220 | 113 | 211 | 176 | 316 | 262 | – | – | – | 1 | 4 | 188 | 68 | 121 | 116 |
Endoplasmic Reticulum | 5 | 14 | 17 | 1 | 3 | 8 | 2 | 5 | 9 | 3 | 5 | 4 | 10 | 8 | 12 | – | – | – | 3 | 4 | 5 | 3 | 6 | 6 |
Endosome | 34 | 8 | 8 | 2 | 17 | 9 | 12 | 18 | 2 | 2 | 0 | 0 | 16 | 31 | 15 | – | – | – | 70 | 38 | 19 | 15 | 42 | 46 |
Golgi | 13 | 57 | 18 | 9 | 9 | 1 | 1 | 3 | 0 | 1 | 1 | 0 | 21 | 30 | 21 | – | – | – | 47 | 45 | 20 | 20 | 36 | 28 |
Mitochondria | 188 | 92 | 79 | 57 | 94 | 191 | 253 | 257 | 179 | 281 | 152 | 338 | 33 | 50 | 19 | – | – | – | 141 | 118 | 81 | 38 | 104 | 72 |
Nucleus | 2 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 2 | 2 | 3 | 3 | 1 | 0 | 0 | – | – | – | 1 | 0 | 0 | 0 | 0 | 1 |
Nuclear Periphery | 1 | 0 | 1 | 0 | 1 | 6 | 4 | 4 | 5 | 5 | 5 | 9 | 0 | 0 | 0 | – | – | – | 7 | 2 | 2 | 1 | 5 | 3 |
Nucleolus | 19 | 2 | 0 | 0 | 2 | 4 | 5 | 8 | 3 | 7 | 4 | 4 | 0 | 0 | 0 | – | – | – | 0 | 1 | 0 | 0 | 2 | 2 |
Peroxisomes | 3 | 3 | 1 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 6 | 1 | – | – | – | 6 | 5 | 7 | 4 | 6 | 9 |
SpindlePole | 2 | 1 | 0 | 1 | 1 | 1 | 1 | 3 | 3 | 1 | 3 | 5 | 0 | 2 | 0 | – | – | – | 6 | 3 | 7 | 4 | 12 | 12 |
Vac/Vac Membrane | 63 | 65 | 10 | 2 | 18 | 23 | 32 | 27 | 9 | 14 | 5 | 10 | 18 | 39 | 34 | – | – | – | 12 | 31 | 3 | 2 | 9 | 9 |
Unique Cell Count | 276 | 228 | 344 | 180 | 360 | 437 | 578 | 557 | 289 | 437 | 229 | 471 | 264 | 457 | 352 | 320 | 288 | 359 | 173 | 406 | 372 | |||
Labelled Cell Count | 358 | 309 | 400 | 206 | 407 | 526 | 673 | 669 | 374 | 558 | 297 | 606 | 296 | 498 | 374 | 320 | 288 | 359 | 173 | 406 | 372 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.1 | 9.3 | 5.7 | 5.5 | 5.4 | 4.5 | 4.5 | 4.6 | 4.2 | 3.9 | 3.9 | 3.8 | 7.8 | 7.7 | 7.5 | – | – | – | 7.2 | 7.4 | 7.9 |
Std Deviation (1e-4) | 1.6 | 1.6 | 1.6 | 2.0 | 1.9 | 1.7 | 1.7 | 1.6 | 1.5 | 1.4 | 1.4 | 1.3 | 2.5 | 2.2 | 2.2 | – | – | – | 2.3 | 2.6 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.04 | -0.07 | -0.33 | -0.36 | -0.3 | -0.43 | -0.56 | -0.55 | -0.57 | 0.45 | 0.43 | 0.39 | – | – | – | 0.33 | 0.37 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.8 | -2.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.4 | -3.8 | -3.6 | – | – | – |
Bud | -0.6 | -2.6 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -2.1 | -2.7 | -3.4 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | -0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.3 | 0.6 | 0.7 | – | – | – |
Cytoplasm | 0.5 | 0.4 | -1.4 | -2.3 | -3.1 | -4.1 | -4.9 | -4.4 | -6.5 | -0.3 | 0.4 | 1.9 | – | – | – |
Endoplasmic Reticulum | -2.6 | -3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -0.7 | -2.6 | -1.0 | – | – | – |
Endosome | 0 | 1.7 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.3 | 2.9 | 1.4 | – | – | – |
Golgi | -0.1 | -1.9 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.4 | 0.8 | 0.4 | – | – | – |
Mitochondria | 2.2 | 1.0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -3.3 | -4.6 | -6.7 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0 | 1.4 | 1.6 | 1.9 | 1.4 | 0.2 | 0.2 | -0.5 | -0.7 | 2.3 | 3.3 | 3.7 | – | – | – |