Standard name
Description Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0.12 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0 0 0.06 0 0.05 0 0.05 0 0 0 0.05 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Endosome 0.07 0 0.08 0 0.06 0.05 0 0 0 0 0 0 0 0.05 0 – – – 0.05 0 0.06 0 0 0
Golgi 0 0 0.24 0.1 0.18 0.05 0 0.12 0 0 0 0 0 0.16 0.33 – – – 0.05 0.05 0 0.1 0.14 0.11
Mitochondria 0.91 0.97 0.73 0.86 0.69 0.78 0.77 0.68 0.89 0.87 0.88 0.89 0.89 0.86 0.77 – – – 0.84 0.82 0.78 0.65 0.69 0.69
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.05 0.13 0.07 0.06 0.05 0.06 0 0.09 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0.05 0 0 0 0.05 0.1 0.09 0.08 0.11 0.12 0.07 0.12 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0.09 0 0 0 0 0 0 0 0 0 0 0.05 0.07 – – – 0 0 0.05 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0.11 0.09 0.06 0.18 0.11 0.09 0.12 0 0 0 0 0 0.11 0.07 – – – 0 0 0 0.05 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 0 1 1 3 0 2 0 2 0 – – – 0 2 1 3 5 5
Bud 0 2 3 3 2 2 7 3 7 6 3 2 0 2 1 – – – 0 0 1 0 2 2
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 1 0 0 0 0 0 1 1 1 0 4 0 0 0 – – – – – – – – –
Cell Periphery 1 15 2 6 5 3 1 3 0 4 0 2 1 3 4 – – – 0 0 0 0 0 0
Cytoplasm 1 1 2 10 7 10 19 11 12 9 11 4 2 6 9 – – – 0 0 1 2 0 0
Endoplasmic Reticulum 0 1 4 0 0 2 0 1 0 0 0 0 0 0 3 – – – 0 0 0 1 0 0
Endosome 14 0 11 6 12 12 1 13 0 1 3 2 2 9 3 – – – 9 4 9 3 3 5
Golgi 6 2 33 15 37 14 7 42 1 6 2 5 2 27 56 – – – 9 7 6 8 26 21
Mitochondria 173 124 102 125 142 205 233 233 237 296 184 272 62 150 131 – – – 168 108 128 57 132 136
Nucleus 3 0 0 1 0 1 3 2 4 0 0 0 3 0 0 – – – 0 0 0 0 2 1
Nuclear Periphery 6 5 4 3 11 34 21 20 14 19 5 26 2 2 4 – – – 0 0 1 1 1 2
Nucleolus 9 1 2 2 10 27 27 27 30 39 15 36 3 3 1 – – – 0 0 0 0 3 4
Peroxisomes 1 1 13 5 9 4 7 7 1 4 0 4 2 9 12 – – – 5 5 8 1 3 6
SpindlePole 4 0 2 0 4 2 0 4 0 5 1 3 1 1 1 – – – 0 0 1 0 0 0
Vac/Vac Membrane 34 14 12 9 37 30 28 43 8 15 9 8 3 19 12 – – – 3 3 0 4 7 6
Unique Cell Count 191 128 140 145 206 264 302 345 265 339 209 305 70 174 170 202 133 165 89 192 198
Labelled Cell Count 252 167 190 186 276 346 354 411 316 408 233 370 83 233 237 202 133 165 89 192 198


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 7.2 6.6 8.9 8.3 12.0 11.6 12.4 14.4 11.0 13.2 12.0 12.7 9.2 11.3 11.6 – – – 15.9 14.4 13.8
Std Deviation (1e-4) 1.7 1.2 1.9 1.8 3.5 2.9 3.6 4.7 4.1 4.7 5.0 5.7 4.4 6.3 5.0 – – – 16.0 13.7 12.5
Intensity Change (Log2) – – – -0.1 0.43 0.38 0.48 0.69 0.31 0.57 0.43 0.52 0.05 0.34 0.39 – – – 0.84 0.7 0.64


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 2.3 0 0 2.2 0 0 0 1.9 0 0 0 0 – – –
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Endosome -1.3 -0.7 -1.4 0 -1.9 0 0 -3.0 0 0 -1.0 -2.6 – – –
Golgi -3.0 -1.3 -5.4 -7.2 -3.1 -8.0 -7.9 -6.9 -7.7 -3.8 -1.8 1.8 – – –
Mitochondria 2.8 -0.8 1.1 1.0 -1.1 4.3 3.8 3.6 4.4 2.6 3.0 0.9 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 1.1 3.3 1.7 1.4 1.1 1.3 0 2.2 0 0 0 – – –
Nucleolus 0 0 3.3 3.0 2.7 3.5 3.6 2.4 3.6 0 0 0 – – –
Peroxisomes -2.0 -1.8 -3.7 -3.3 -3.6 0 0 -4.5 -4.1 -1.7 -1.4 -0.7 – – –
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Vacuole -0.8 2.5 0.9 0.2 1.2 -2.5 -1.8 -1.6 -2.8 -1.1 0.7 -0.5 – – –