Standard name | |
---|---|
Human Ortholog | |
Description | One of two mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 6 | 2 | 0 | 6 | 3 | 0 | 3 | 6 | 4 | 5 | 24 | 21 |
Bud | 1 | 4 | 3 | 4 | 3 | 3 | 9 | – | 12 | 15 | 22 | 15 | 8 | 2 | 3 | 1 | 0 | 0 | 0 | 3 | 0 | 1 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 9 | 7 |
Bud Site | 1 | 1 | 0 | 1 | 0 | 2 | 2 | – | 2 | 6 | 6 | 9 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 6 | 11 | 6 | 11 | 3 | 1 | 6 | – | 5 | 4 | 1 | 2 | 6 | 4 | 3 | 3 | 7 | 1 | 0 | 1 | 0 | 1 | 1 | 0 |
Cytoplasm | 6 | 31 | 17 | 24 | 26 | 14 | 25 | – | 20 | 10 | 16 | 18 | 34 | 77 | 138 | 5 | 1 | 6 | 0 | 2 | 4 | 1 | 5 | 4 |
Endoplasmic Reticulum | 5 | 27 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 2 | 3 | 10 | 2 | 1 | 2 | 4 | 2 | 1 | 3 | 1 | 2 | 3 | 8 |
Endosome | 16 | 2 | 14 | 39 | 39 | 6 | 53 | – | 6 | 14 | 13 | 8 | 42 | 46 | 96 | 2 | 1 | 2 | 28 | 19 | 6 | 24 | 35 | 42 |
Golgi | 7 | 7 | 6 | 0 | 4 | 0 | 0 | – | 0 | 0 | 0 | 1 | 4 | 2 | 5 | 16 | 18 | 11 | 15 | 19 | 5 | 9 | 18 | 19 |
Mitochondria | 206 | 200 | 39 | 101 | 53 | 121 | 156 | – | 175 | 210 | 290 | 299 | 163 | 139 | 129 | 7 | 3 | 1 | 181 | 209 | 109 | 205 | 221 | 199 |
Nucleus | 0 | 2 | 0 | 1 | 2 | 7 | 9 | – | 4 | 2 | 6 | 6 | 4 | 4 | 2 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 7 | 3 | 0 | 0 | 0 | 0 | 3 | – | 5 | 1 | 4 | 2 | 1 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 0 | 2 | 1 | 5 |
Nucleolus | 2 | 1 | 0 | 1 | 0 | 5 | 7 | – | 3 | 3 | 14 | 6 | 1 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 3 |
Peroxisomes | 4 | 1 | 9 | 25 | 14 | 1 | 11 | – | 7 | 8 | 3 | 2 | 8 | 8 | 61 | 0 | 0 | 1 | 10 | 5 | 4 | 12 | 16 | 29 |
SpindlePole | 3 | 2 | 8 | 16 | 21 | 8 | 21 | – | 7 | 25 | 20 | 17 | 8 | 18 | 7 | 1 | 1 | 1 | 3 | 1 | 0 | 3 | 6 | 10 |
Vac/Vac Membrane | 48 | 30 | 11 | 18 | 32 | 26 | 48 | – | 26 | 27 | 18 | 17 | 27 | 61 | 60 | 13 | 10 | 9 | 8 | 20 | 1 | 9 | 13 | 18 |
Unique Cell Count | 244 | 255 | 87 | 165 | 151 | 154 | 258 | 216 | 250 | 328 | 340 | 264 | 299 | 408 | 48 | 37 | 38 | 264 | 301 | 143 | 288 | 375 | 384 | |
Labelled Cell Count | 313 | 325 | 113 | 241 | 197 | 194 | 350 | 273 | 327 | 417 | 405 | 322 | 365 | 506 | 58 | 52 | 42 | 264 | 301 | 143 | 288 | 375 | 384 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.3 | 6.8 | 6.1 | 7.0 | 5.1 | 6.0 | – | 5.3 | 5.4 | 5.3 | 5.5 | 5.6 | 6.3 | 6.1 | 8.0 | 8.7 | 8.3 | 5.2 | 5.3 | 5.6 |
Std Deviation (1e-4) | 0.9 | 0.9 | 2.4 | 2.0 | 5.2 | 2.4 | 2.0 | – | 1.6 | 1.4 | 1.3 | 1.5 | 1.1 | 2.8 | 1.5 | 1.7 | 1.5 | 1.8 | 1.7 | 1.7 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.16 | 0.03 | -0.43 | -0.19 | – | -0.36 | -0.35 | -0.37 | -0.31 | -0.28 | -0.11 | -0.16 | 0.24 | 0.35 | 0.28 | -0.39 | -0.37 | -0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | -0.4 | -2.3 | -2.4 | – | -2.5 | -4.6 | -4.5 | -4.3 | -1.5 | 1.2 | 2.6 | -1.4 | -2.4 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.4 | 1.7 | -3.3 | 0.9 | – | -4.2 | -3.1 | -4.1 | 0 | 0 | -0.2 | 1.5 | -2.1 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 5.4 | 3.3 |
Mitochondria | 2.5 | -1.5 | 5.3 | 2.5 | – | 6.3 | 7.2 | 8.9 | 8.8 | 2.8 | 0.3 | -2.4 | -3.5 | -4.0 | -4.7 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.1 | -0.3 | 0 | -2.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 | 0 |
SpindlePole | 0.1 | 1.1 | -1.2 | -0.3 | – | 0 | 0.2 | -1.0 | -1.5 | 0 | -1.0 | 0 | 0 | 0 | 0 |
Vacuole | -0.4 | 1.7 | 0.9 | 1.3 | – | -0.1 | -0.5 | -2.3 | -2.6 | -0.6 | 1.6 | 0.5 | 2.1 | 2.0 | 1.5 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 0.8769 | 0.861 | 0.6702 | 0.585 | 0.2835 | 0.8103 | 2.8328 | 2.3099 | 2.6637 | 2.3587 | 2.5379 | 2.5133 | 4.519 | 4.6134 | 4.1172 | 4.5599 | 3.6952 | 4.4764 |
Actin | 0.0251 | 0.0089 | 0.008 | 0.0058 | 0.0126 | 0.0029 | 0.0384 | 0.0097 | 0.0218 | 0.023 | 0.0053 | 0.004 | 0.0344 | 0.04 | 0.0258 | 0.0614 | 0.0374 | 0.0047 |
Bud | 0.0014 | 0.0127 | 0.0105 | 0.0019 | 0.001 | 0.0045 | 0.0016 | 0.0029 | 0.003 | 0.0066 | 0.002 | 0.0017 | 0.0021 | 0.0039 | 0.0026 | 0.0021 | 0.0005 | 0.0016 |
Bud Neck | 0.002 | 0.0017 | 0.0052 | 0.0127 | 0.001 | 0.0013 | 0.0018 | 0.0013 | 0.0021 | 0.0013 | 0.0011 | 0.0023 | 0.0027 | 0.0016 | 0.0032 | 0.0007 | 0.0015 | 0.0011 |
Bud Periphery | 0.0017 | 0.0106 | 0.0077 | 0.0043 | 0.0026 | 0.0032 | 0.0023 | 0.0043 | 0.006 | 0.0107 | 0.0031 | 0.0046 | 0.0025 | 0.0147 | 0.0051 | 0.0067 | 0.0009 | 0.0063 |
Bud Site | 0.006 | 0.0364 | 0.0106 | 0.0075 | 0.0018 | 0.0009 | 0.0132 | 0.0353 | 0.0118 | 0.007 | 0.0038 | 0.0012 | 0.0246 | 0.0501 | 0.0083 | 0.0011 | 0.0026 | 0.0008 |
Cell Periphery | 0.0008 | 0.0017 | 0.0023 | 0.0008 | 0.0006 | 0.0009 | 0.0008 | 0.001 | 0.0013 | 0.0007 | 0.0005 | 0.001 | 0.0022 | 0.0065 | 0.001 | 0.0004 | 0.0003 | 0.0018 |
Cytoplasm | 0.014 | 0.0109 | 0.0142 | 0.012 | 0.0049 | 0.0147 | 0.0173 | 0.0131 | 0.017 | 0.0224 | 0.0317 | 0.0116 | 0.0271 | 0.0174 | 0.0224 | 0.0065 | 0.0019 | 0.0144 |
Cytoplasmic Foci | 0.0434 | 0.0535 | 0.0475 | 0.0681 | 0.0509 | 0.0433 | 0.0424 | 0.0515 | 0.0411 | 0.0376 | 0.0419 | 0.027 | 0.0491 | 0.0439 | 0.0308 | 0.0195 | 0.0349 | 0.0515 |
Eisosomes | 0.0009 | 0.0003 | 0.0005 | 0.0003 | 0.0002 | 0.0003 | 0.0008 | 0.0002 | 0.0004 | 0.0004 | 0.0002 | 0.0002 | 0.0034 | 0.0021 | 0.0009 | 0.0004 | 0.0005 | 0.0004 |
Endoplasmic Reticulum | 0.0031 | 0.0009 | 0.0025 | 0.0009 | 0.0008 | 0.0018 | 0.0052 | 0.0032 | 0.0029 | 0.0252 | 0.0031 | 0.0018 | 0.0119 | 0.0017 | 0.0114 | 0.0011 | 0.0017 | 0.0042 |
Endosome | 0.1685 | 0.0811 | 0.1151 | 0.1348 | 0.0934 | 0.0726 | 0.1461 | 0.1794 | 0.1205 | 0.0886 | 0.2039 | 0.1059 | 0.0447 | 0.1213 | 0.1138 | 0.1326 | 0.0937 | 0.0965 |
Golgi | 0.0685 | 0.0868 | 0.054 | 0.0704 | 0.0577 | 0.0639 | 0.078 | 0.0947 | 0.0736 | 0.0792 | 0.0985 | 0.0661 | 0.0393 | 0.0886 | 0.0758 | 0.0725 | 0.0828 | 0.0599 |
Lipid Particles | 0.0365 | 0.0154 | 0.0143 | 0.0133 | 0.0075 | 0.0132 | 0.0251 | 0.0125 | 0.0103 | 0.0216 | 0.0361 | 0.0094 | 0.0847 | 0.0043 | 0.0118 | 0.0016 | 0.0504 | 0.0219 |
Mitochondria | 0.543 | 0.6104 | 0.6211 | 0.565 | 0.7212 | 0.7135 | 0.5383 | 0.522 | 0.6309 | 0.6064 | 0.4796 | 0.7315 | 0.5318 | 0.5468 | 0.5854 | 0.6703 | 0.6144 | 0.6831 |
None | 0.0159 | 0.0196 | 0.0335 | 0.0127 | 0.0005 | 0.0088 | 0.0075 | 0.0063 | 0.0012 | 0.0051 | 0.0058 | 0.0014 | 0.0506 | 0.0242 | 0.0508 | 0.0008 | 0.0005 | 0.0048 |
Nuclear Periphery | 0.0047 | 0.0003 | 0.0031 | 0.0007 | 0.0004 | 0.0009 | 0.0118 | 0.0018 | 0.0022 | 0.0087 | 0.0264 | 0.002 | 0.0193 | 0.0003 | 0.0047 | 0.0005 | 0.0015 | 0.0013 |
Nucleolus | 0.0042 | 0.001 | 0.0019 | 0.0087 | 0.0002 | 0.0009 | 0.0046 | 0.0009 | 0.0008 | 0.0004 | 0.0022 | 0.0003 | 0.0029 | 0.0006 | 0.0004 | 0.0002 | 0.0001 | 0.0004 |
Nucleus | 0.0067 | 0.0009 | 0.0021 | 0.0088 | 0.0002 | 0.0013 | 0.0093 | 0.0013 | 0.0014 | 0.0014 | 0.0069 | 0.0006 | 0.0139 | 0.0006 | 0.0014 | 0.0003 | 0.0002 | 0.0007 |
Peroxisomes | 0.0235 | 0.0359 | 0.0233 | 0.0346 | 0.0344 | 0.0365 | 0.0122 | 0.0206 | 0.0217 | 0.0324 | 0.0209 | 0.0123 | 0.0331 | 0.0212 | 0.0175 | 0.0086 | 0.0693 | 0.0177 |
Punctate Nuclear | 0.0054 | 0.0003 | 0.0023 | 0.0057 | 0.0002 | 0.0026 | 0.0144 | 0.0004 | 0.0007 | 0.0013 | 0.0027 | 0.0008 | 0.0103 | 0.0002 | 0.0019 | 0.0002 | 0.0006 | 0.0027 |
Vacuole | 0.016 | 0.0072 | 0.0129 | 0.022 | 0.004 | 0.0067 | 0.0185 | 0.0266 | 0.0201 | 0.0116 | 0.0115 | 0.0076 | 0.0064 | 0.0071 | 0.0149 | 0.0072 | 0.0015 | 0.0139 |
Vacuole Periphery | 0.009 | 0.0037 | 0.0075 | 0.0088 | 0.0037 | 0.0056 | 0.0104 | 0.0113 | 0.0091 | 0.0084 | 0.0128 | 0.0069 | 0.0032 | 0.0028 | 0.0099 | 0.0053 | 0.0027 | 0.0103 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 55.4342 | 47.195 | 46.6943 | 45.5339 | 54.7603 | 43.971 | 41.3563 | 37.5748 | 45.672 | 47.5288 |
Translational Efficiency | 0.9369 | 0.9661 | 0.9639 | 1.0521 | 0.6968 | 0.9674 | 1.0713 | 1.0902 | 0.9854 | 0.9245 |
Dataset | Hit |
---|---|
Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
177 | 875 | 142 | 2861 | 1676 | 2491 | 1733 | 2530 | 1853 | 3366 | 1875 | 5391 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 642.09 | 781.52 | 1021.94 | 1047.80 | 684.00 | 822.38 | 862.79 | 1095.33 | 680.00 | 811.76 | 874.84 | 1070.11 |
Standard Deviation | 86.73 | 117.16 | 118.10 | 173.93 | 80.87 | 111.47 | 160.52 | 167.21 | 82.37 | 114.39 | 163.23 | 172.45 |
Intensity Change Log 2 | — | 0.283507 | 0.670463 | 0.706516 | — | 0.265809 | 0.335013 | 0.679297 | — | 0.274406 | 0.507179 | 0.692541 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000892 | 0.003148 | 0.012236 | 0.011234 | 0.000994 | 0.000840 | 0.005231 | 0.002192 | 0.000984 | 0.001440 | 0.005762 | 0.006990 |
Bud Neck | 0.042293 | 0.016739 | 0.016153 | 0.001871 | 0.020601 | 0.010571 | 0.007172 | 0.001990 | 0.022673 | 0.012175 | 0.007852 | 0.001927 |
Bud Site | 0.032428 | 0.049807 | 0.077687 | 0.059882 | 0.025160 | 0.021102 | 0.113297* | 0.057388 | 0.025854 | 0.028564 | 0.110600* | 0.058711 |
Cell Periphery | 0.002922 | 0.000730 | 0.000433 | 0.004274 | 0.000371 | 0.000366 | 0.001511 | 0.000554 | 0.000614 | 0.000461 | 0.001429 | 0.002528 |
Cytoplasm | 0.089133 | 0.021942 | 0.031920 | 0.014208 | 0.044098 | 0.010841 | 0.026831 | 0.020054 | 0.048400 | 0.013726 | 0.027217 | 0.016951 |
Cytoplasmic Foci | 0.165761 | 0.108415 | 0.146659 | 0.012469 | 0.145648 | 0.054632 | 0.055390 | 0.032690 | 0.147569 | 0.068613 | 0.062302 | 0.021959 |
Eisosomes | 0.000695 | 0.000161 | 0.000175 | 0.000136 | 0.000188 | 0.000083 | 0.000107 | 0.000058 | 0.000236 | 0.000103 | 0.000113 | 0.000100 |
Endoplasmic Reticulum | 0.002431 | 0.001414 | 0.006372 | 0.001933 | 0.002634 | 0.000809 | 0.003085 | 0.000776 | 0.002614 | 0.000966 | 0.003334 | 0.001390 |
Endosome | 0.043419 | 0.121414 | 0.143087 | 0.024101 | 0.083983 | 0.094204 | 0.086365 | 0.050237 | 0.080108 | 0.101277 | 0.090661 | 0.036367 |
Golgi | 0.054362 | 0.147518* | 0.124344* | 0.072306 | 0.092124 | 0.121627* | 0.102806 | 0.147943* | 0.088517 | 0.128357* | 0.104438 | 0.107802 |
Lipid Particles | 0.027178 | 0.004043 | 0.012665 | 0.000167 | 0.012213 | 0.003960 | 0.001085 | 0.000187 | 0.013642 | 0.003982 | 0.001962 | 0.000176 |
Mitochondria | 0.400959* | 0.385375* | 0.089097 | 0.737566* | 0.469043* | 0.584983* | 0.365683* | 0.528450* | 0.462540* | 0.533094* | 0.344737* | 0.639428* |
Mitotic Spindle | 0.001762 | 0.017409 | 0.083874 | 0.010879 | 0.007866 | 0.012205 | 0.042851 | 0.041038 | 0.007283 | 0.013558 | 0.045957 | 0.025032 |
None | 0.001398 | 0.000606 | 0.000379 | 0.000606 | 0.001043 | 0.001081 | 0.001072 | 0.000548 | 0.001077 | 0.000957 | 0.001019 | 0.000578 |
Nuclear Periphery | 0.000837 | 0.000155 | 0.001399 | 0.000040 | 0.000530 | 0.000133 | 0.000329 | 0.000222 | 0.000559 | 0.000139 | 0.000410 | 0.000125 |
Nuclear Periphery Foci | 0.000312 | 0.000412 | 0.003790 | 0.000057 | 0.000406 | 0.000422 | 0.000822 | 0.000305 | 0.000397 | 0.000420 | 0.001047 | 0.000173 |
Nucleolus | 0.002268 | 0.002613 | 0.009539 | 0.000031 | 0.001293 | 0.000695 | 0.001930 | 0.000082 | 0.001386 | 0.001194 | 0.002506 | 0.000055 |
Nucleus | 0.045104 | 0.007036 | 0.014765 | 0.000125 | 0.007520 | 0.002272 | 0.003715 | 0.000306 | 0.011110 | 0.003511 | 0.004552 | 0.000210 |
Peroxisomes | 0.049665 | 0.050342 | 0.084092 | 0.005023 | 0.032835 | 0.017063 | 0.021093 | 0.005199 | 0.034442 | 0.025714 | 0.025864 | 0.005106 |
Vacuole | 0.021372 | 0.037086 | 0.091303 | 0.017851 | 0.030468 | 0.037721 | 0.089874 | 0.050873 | 0.029599 | 0.037556 | 0.089982 | 0.033348 |
Vacuole Periphery | 0.014808 | 0.023634 | 0.050032 | 0.025243 | 0.020982 | 0.024390 | 0.069750 | 0.058909 | 0.020393 | 0.024193 | 0.068257 | 0.041043 |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -2.99 | -3.84 | -8.87 | -6.26 | 0.33 | 0.59 | -5.51 | -3.58 | -3.90 | 3.75 | -1.66 | -6.40 | -9.57 | -8.58 | -1.28 |
Bud Neck | 3.31 | 3.23 | 5.43 | 7.35 | 4.56 | 5.65 | 8.30 | 12.26 | 9.10 | 8.94 | 5.92 | 8.95 | 13.37 | 11.69 | 10.08 |
Bud Site | -2.15 | -3.62 | -3.66 | -2.21 | 1.68 | 1.83 | -18.98 | -10.35 | -12.44 | 11.06 | -1.22 | -19.23 | -13.12 | -13.01 | 11.61 |
Cell Periphery | 2.17 | 2.47 | -1.23 | -7.40 | -8.14 | 0.08 | -4.24 | -3.79 | -3.67 | 3.55 | 1.40 | -3.05 | -7.10 | -8.18 | -3.16 |
Cytoplasm | 5.38 | 3.60 | 6.14 | 3.25 | 2.36 | 11.62 | 5.01 | 7.95 | -6.00 | 3.17 | 12.00 | 6.13 | 11.01 | -2.65 | 5.42 |
Cytoplasmic Foci | 3.96 | 1.22 | 11.26 | 21.04 | 12.72 | 20.48 | 20.00 | 26.66 | 10.21 | 9.74 | 18.31 | 19.38 | 31.79 | 24.16 | 18.49 |
Eisosomes | 5.35 | 5.10 | 5.61 | 2.11 | 1.86 | 7.96 | 5.87 | 10.03 | 6.48 | 9.31 | 8.46 | 7.68 | 8.60 | 0.69 | 2.06 |
Endoplasmic Reticulum | 1.40 | -2.59 | 0.70 | -1.71 | 3.18 | 3.39 | -0.79 | 3.68 | 0.14 | 6.49 | 3.41 | -1.35 | 2.58 | -2.17 | 5.42 |
Endosome | -9.78 | -7.60 | 3.12 | 18.75 | 10.32 | -2.42 | -0.52 | 9.43 | 13.96 | 11.59 | -5.49 | -2.56 | 14.01 | 25.01 | 19.31 |
Golgi | -9.09 | -4.12 | -2.11 | 11.12 | 3.41 | -6.10 | -2.05 | -10.44 | -4.91 | -8.24 | -9.05 | -3.20 | -4.78 | 5.31 | -0.90 |
Lipid Particles | 3.70 | 1.98 | 4.36 | 6.05 | 3.63 | 6.00 | 9.23 | 10.10 | 6.20 | 5.00 | 7.16 | 9.14 | 10.94 | 7.91 | 5.65 |
Mitochondria | 0.54 | 10.39 | -12.15 | -27.84* | -46.29* | -10.95 | 8.98 | -5.42 | 5.97 | -15.35 | -7.12 | 10.68 | -19.08 | -14.22 | -32.95* |
Mitotic Spindle | -8.43 | -6.38 | -10.36 | 3.44 | 5.66 | -3.46 | -12.91 | -14.69 | -12.55 | 0.50 | -5.58 | -14.35 | -13.49 | -8.42 | 7.42 |
None | 1.12 | 1.48 | 1.17 | 0.21 | -2.29 | -0.12 | -0.02 | 3.49 | 1.91 | 1.91 | 0.44 | 0.26 | 3.41 | 1.84 | 1.73 |
Nuclear Periphery | 2.34 | -1.11 | 2.76 | 2.91 | 3.25 | 2.63 | 1.19 | 1.96 | -1.57 | 1.19 | 3.02 | 0.93 | 3.11 | 0.46 | 3.53 |
Nuclear Periphery Foci | -0.84 | -2.98 | 2.82 | 4.74 | 3.19 | -0.07 | -2.81 | 0.88 | 0.47 | 3.53 | -0.14 | -4.02 | 2.71 | 1.41 | 5.74 |
Nucleolus | -0.39 | -1.50 | 3.30 | 3.05 | 2.01 | 2.16 | -1.01 | 5.18 | 5.25 | 3.26 | 0.46 | -1.62 | 6.02 | 4.84 | 3.86 |
Nucleus | 3.43 | 2.54 | 4.12 | 3.85 | 3.96 | 4.70 | 2.86 | 7.33 | 3.77 | 4.95 | 4.97 | 3.94 | 7.84 | 5.45 | 6.21 |
Peroxisomes | -0.11 | -2.34 | 6.15 | 12.00 | 6.30 | 6.34 | 4.24 | 12.25 | 8.86 | 8.75 | 3.50 | 3.08 | 13.78 | 14.70 | 10.74 |
Vacuole | -3.51 | -6.76 | 1.02 | 6.22 | 7.40 | -2.98 | -16.96 | -8.10 | -5.32 | 10.66 | -3.68 | -18.21 | -2.11 | 2.09 | 18.00 |
Vacuole Periphery | -2.77 | -3.92 | -3.71 | -0.76 | 2.81 | -2.31 | -16.35 | -15.85 | -14.67 | 3.03 | -2.81 | -16.87 | -13.04 | -11.22 | 9.28 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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