Standard name | |
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Description | One of two mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1 |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 3 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 6 | 2 | 0 | 6 | 3 | 0 | 3 | 6 | 4 | 5 | 24 | 21 |
Bud | 1 | 4 | 3 | 4 | 3 | 3 | 9 | – | 12 | 15 | 22 | 15 | 8 | 2 | 3 | 1 | 0 | 0 | 0 | 3 | 0 | 1 | 5 | 5 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 9 | 7 |
Bud Site | 1 | 1 | 0 | 1 | 0 | 2 | 2 | – | 2 | 6 | 6 | 9 | 0 | 0 | 0 | 0 | 0 | 1 | – | – | – | – | – | – |
Cell Periphery | 6 | 11 | 6 | 11 | 3 | 1 | 6 | – | 5 | 4 | 1 | 2 | 6 | 4 | 3 | 3 | 7 | 1 | 0 | 1 | 0 | 1 | 1 | 0 |
Cytoplasm | 6 | 31 | 17 | 24 | 26 | 14 | 25 | – | 20 | 10 | 16 | 18 | 34 | 77 | 138 | 5 | 1 | 6 | 0 | 2 | 4 | 1 | 5 | 4 |
Endoplasmic Reticulum | 5 | 27 | 0 | 0 | 0 | 0 | 0 | – | 1 | 2 | 2 | 3 | 10 | 2 | 1 | 2 | 4 | 2 | 1 | 3 | 1 | 2 | 3 | 8 |
Endosome | 16 | 2 | 14 | 39 | 39 | 6 | 53 | – | 6 | 14 | 13 | 8 | 42 | 46 | 96 | 2 | 1 | 2 | 28 | 19 | 6 | 24 | 35 | 42 |
Golgi | 7 | 7 | 6 | 0 | 4 | 0 | 0 | – | 0 | 0 | 0 | 1 | 4 | 2 | 5 | 16 | 18 | 11 | 15 | 19 | 5 | 9 | 18 | 19 |
Mitochondria | 206 | 200 | 39 | 101 | 53 | 121 | 156 | – | 175 | 210 | 290 | 299 | 163 | 139 | 129 | 7 | 3 | 1 | 181 | 209 | 109 | 205 | 221 | 199 |
Nucleus | 0 | 2 | 0 | 1 | 2 | 7 | 9 | – | 4 | 2 | 6 | 6 | 4 | 4 | 2 | 1 | 1 | 4 | 0 | 0 | 0 | 0 | 1 | 1 |
Nuclear Periphery | 7 | 3 | 0 | 0 | 0 | 0 | 3 | – | 5 | 1 | 4 | 2 | 1 | 0 | 0 | 0 | 1 | 3 | 2 | 2 | 0 | 2 | 1 | 5 |
Nucleolus | 2 | 1 | 0 | 1 | 0 | 5 | 7 | – | 3 | 3 | 14 | 6 | 1 | 0 | 1 | 1 | 2 | 0 | 1 | 0 | 0 | 0 | 2 | 3 |
Peroxisomes | 4 | 1 | 9 | 25 | 14 | 1 | 11 | – | 7 | 8 | 3 | 2 | 8 | 8 | 61 | 0 | 0 | 1 | 10 | 5 | 4 | 12 | 16 | 29 |
SpindlePole | 3 | 2 | 8 | 16 | 21 | 8 | 21 | – | 7 | 25 | 20 | 17 | 8 | 18 | 7 | 1 | 1 | 1 | 3 | 1 | 0 | 3 | 6 | 10 |
Vac/Vac Membrane | 48 | 30 | 11 | 18 | 32 | 26 | 48 | – | 26 | 27 | 18 | 17 | 27 | 61 | 60 | 13 | 10 | 9 | 8 | 20 | 1 | 9 | 13 | 18 |
Unique Cell Count | 244 | 255 | 87 | 165 | 151 | 154 | 258 | 216 | 250 | 328 | 340 | 264 | 299 | 408 | 48 | 37 | 38 | 264 | 301 | 143 | 288 | 375 | 384 | |
Labelled Cell Count | 313 | 325 | 113 | 241 | 197 | 194 | 350 | 273 | 327 | 417 | 405 | 322 | 365 | 506 | 58 | 52 | 42 | 264 | 301 | 143 | 288 | 375 | 384 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 5.3 | 5.3 | 6.8 | 6.1 | 7.0 | 5.1 | 6.0 | – | 5.3 | 5.4 | 5.3 | 5.5 | 5.6 | 6.3 | 6.1 | 8.0 | 8.7 | 8.3 | 5.2 | 5.3 | 5.6 |
Std Deviation (1e-4) | 0.9 | 0.9 | 2.4 | 2.0 | 5.2 | 2.4 | 2.0 | – | 1.6 | 1.4 | 1.3 | 1.5 | 1.1 | 2.8 | 1.5 | 1.7 | 1.5 | 1.8 | 1.7 | 1.7 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.16 | 0.03 | -0.43 | -0.19 | – | -0.36 | -0.35 | -0.37 | -0.31 | -0.28 | -0.11 | -0.16 | 0.24 | 0.35 | 0.28 | -0.39 | -0.37 | -0.28 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | – | 0 | 0 | 1.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | -0.1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.0 | -0.4 | -2.3 | -2.4 | – | -2.5 | -4.6 | -4.5 | -4.3 | -1.5 | 1.2 | 2.6 | -1.4 | -2.4 | -0.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 1.4 | 1.7 | -3.3 | 0.9 | – | -4.2 | -3.1 | -4.1 | 0 | 0 | -0.2 | 1.5 | -2.1 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.0 | 5.4 | 3.3 |
Mitochondria | 2.5 | -1.5 | 5.3 | 2.5 | – | 6.3 | 7.2 | 8.9 | 8.8 | 2.8 | 0.3 | -2.4 | -3.5 | -4.0 | -4.7 |
Nucleus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 1.1 | -0.3 | 0 | -2.1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 1.1 | 0 | 0 | 0 |
SpindlePole | 0.1 | 1.1 | -1.2 | -0.3 | – | 0 | 0.2 | -1.0 | -1.5 | 0 | -1.0 | 0 | 0 | 0 | 0 |
Vacuole | -0.4 | 1.7 | 0.9 | 1.3 | – | -0.1 | -0.5 | -2.3 | -2.6 | -0.6 | 1.6 | 0.5 | 2.1 | 2.0 | 1.5 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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