Standard name
Description One of two mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1
DOWNLOAD

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.12 0.08 0 0 0 0 0 0.07 0.06
Bud 0 0 0 0 0 0 0 – 0.06 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0.07 0.07 0 0 0 – 0 0 0 0 0 0 0 0.06 0.19 0 0 0 0 0 0 0
Cytoplasm 0 0.12 0.2 0.15 0.17 0.09 0.1 – 0.09 0 0.05 0.05 0.13 0.26 0.34 0.1 0 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.11 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.11 0.05 0 0 0 0 0 0
Endosome 0.07 0 0.16 0.24 0.26 0 0.21 – 0 0.06 0 0 0.16 0.15 0.24 0 0 0.05 0.11 0.06 0.05 0.08 0.09 0.11
Golgi 0 0 0.07 0 0 0 0 – 0 0 0 0 0 0 0 0.33 0.49 0.29 0.06 0.06 0 0 0.05 0.05
Mitochondria 0.84 0.78 0.45 0.61 0.35 0.79 0.6 – 0.81 0.84 0.88 0.88 0.62 0.46 0.32 0.15 0.08 0 0.69 0.7 0.76 0.71 0.59 0.52
Nucleus 0 0 0 0 0 0.05 0 – 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0.15 0.09 0 0 – 0 0 0 0 0 0 0.15 0 0 0 0 0 0 0 0 0.08
SpindlePole 0 0 0.09 0.1 0.14 0.05 0.08 – 0 0.1 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.2 0.12 0.13 0.11 0.21 0.17 0.19 – 0.12 0.11 0.05 0.05 0.1 0.2 0.15 0.27 0.27 0.24 0 0.07 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 0 0 0 0 0 – 0 0 1 0 6 2 0 6 3 0 3 6 4 5 24 21
Bud 1 4 3 4 3 3 9 – 12 15 22 15 8 2 3 1 0 0 0 3 0 1 5 5
Bud Neck 0 0 0 0 0 0 0 – 0 0 1 0 0 0 0 0 0 0 0 2 0 1 9 7
Bud Site 1 1 0 1 0 2 2 – 2 6 6 9 0 0 0 0 0 1 – – – – – –
Cell Periphery 6 11 6 11 3 1 6 – 5 4 1 2 6 4 3 3 7 1 0 1 0 1 1 0
Cytoplasm 6 31 17 24 26 14 25 – 20 10 16 18 34 77 138 5 1 6 0 2 4 1 5 4
Endoplasmic Reticulum 5 27 0 0 0 0 0 – 1 2 2 3 10 2 1 2 4 2 1 3 1 2 3 8
Endosome 16 2 14 39 39 6 53 – 6 14 13 8 42 46 96 2 1 2 28 19 6 24 35 42
Golgi 7 7 6 0 4 0 0 – 0 0 0 1 4 2 5 16 18 11 15 19 5 9 18 19
Mitochondria 206 200 39 101 53 121 156 – 175 210 290 299 163 139 129 7 3 1 181 209 109 205 221 199
Nucleus 0 2 0 1 2 7 9 – 4 2 6 6 4 4 2 1 1 4 0 0 0 0 1 1
Nuclear Periphery 7 3 0 0 0 0 3 – 5 1 4 2 1 0 0 0 1 3 2 2 0 2 1 5
Nucleolus 2 1 0 1 0 5 7 – 3 3 14 6 1 0 1 1 2 0 1 0 0 0 2 3
Peroxisomes 4 1 9 25 14 1 11 – 7 8 3 2 8 8 61 0 0 1 10 5 4 12 16 29
SpindlePole 3 2 8 16 21 8 21 – 7 25 20 17 8 18 7 1 1 1 3 1 0 3 6 10
Vac/Vac Membrane 48 30 11 18 32 26 48 – 26 27 18 17 27 61 60 13 10 9 8 20 1 9 13 18
Unique Cell Count 244 255 87 165 151 154 258 216 250 328 340 264 299 408 48 37 38 264 301 143 288 375 384
Labelled Cell Count 313 325 113 241 197 194 350 273 327 417 405 322 365 506 58 52 42 264 301 143 288 375 384


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.3 6.8 6.1 7.0 5.1 6.0 – 5.3 5.4 5.3 5.5 5.6 6.3 6.1 8.0 8.7 8.3 5.2 5.3 5.6
Std Deviation (1e-4) 0.9 0.9 2.4 2.0 5.2 2.4 2.0 – 1.6 1.4 1.3 1.5 1.1 2.8 1.5 1.7 1.5 1.8 1.7 1.7 2.2
Intensity Change (Log2) – – – -0.16 0.03 -0.43 -0.19 – -0.36 -0.35 -0.37 -0.31 -0.28 -0.11 -0.16 0.24 0.35 0.28 -0.39 -0.37 -0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 1.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -0.4 -2.3 -2.4 – -2.5 -4.6 -4.5 -4.3 -1.5 1.2 2.6 -1.4 -2.4 -0.5
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 1.4 1.7 -3.3 0.9 – -4.2 -3.1 -4.1 0 0 -0.2 1.5 -2.1 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 4.0 5.4 3.3
Mitochondria 2.5 -1.5 5.3 2.5 – 6.3 7.2 8.9 8.8 2.8 0.3 -2.4 -3.5 -4.0 -4.7
Nucleus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.1 -0.3 0 -2.1 – 0 0 0 0 0 0 1.1 0 0 0
SpindlePole 0.1 1.1 -1.2 -0.3 – 0 0.2 -1.0 -1.5 0 -1.0 0 0 0 0
Vacuole -0.4 1.7 0.9 1.3 – -0.1 -0.5 -2.3 -2.6 -0.6 1.6 0.5 2.1 2.0 1.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Pth2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available