Standard name
Human Ortholog
Description One of two mitochondrially-localized peptidyl-tRNA hydrolases; negatively regulates the ubiquitin-proteasome pathway via interactions with ubiquitin-like ubiquitin-associated proteins; dispensable for cell growth; see also PTH1

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.12 0.08 0 0 0 0 0 0.07 0.06
Bud 0 0 0 0 0 0 0 0.06 0.06 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0.07 0.07 0 0 0 0 0 0 0 0 0 0 0.06 0.19 0 0 0 0 0 0 0
Cytoplasm 0 0.12 0.2 0.15 0.17 0.09 0.1 0.09 0 0.05 0.05 0.13 0.26 0.34 0.1 0 0.16 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.11 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.05 0 0 0 0 0 0
Endosome 0.07 0 0.16 0.24 0.26 0 0.21 0 0.06 0 0 0.16 0.15 0.24 0 0 0.05 0.11 0.06 0.05 0.08 0.09 0.11
Golgi 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0.33 0.49 0.29 0.06 0.06 0 0 0.05 0.05
Mitochondria 0.84 0.78 0.45 0.61 0.35 0.79 0.6 0.81 0.84 0.88 0.88 0.62 0.46 0.32 0.15 0.08 0 0.69 0.7 0.76 0.71 0.59 0.52
Nucleus 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0.11 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0
Peroxisomes 0 0 0.1 0.15 0.09 0 0 0 0 0 0 0 0 0.15 0 0 0 0 0 0 0 0 0.08
SpindlePole 0 0 0.09 0.1 0.14 0.05 0.08 0 0.1 0.06 0.05 0 0.06 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.2 0.12 0.13 0.11 0.21 0.17 0.19 0.12 0.11 0.05 0.05 0.1 0.2 0.15 0.27 0.27 0.24 0 0.07 0 0 0 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 3 0 0 0 0 0 0 0 1 0 6 2 0 6 3 0 3 6 4 5 24 21
Bud 1 4 3 4 3 3 9 12 15 22 15 8 2 3 1 0 0 0 3 0 1 5 5
Bud Neck 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 2 0 1 9 7
Bud Site 1 1 0 1 0 2 2 2 6 6 9 0 0 0 0 0 1
Cell Periphery 6 11 6 11 3 1 6 5 4 1 2 6 4 3 3 7 1 0 1 0 1 1 0
Cytoplasm 6 31 17 24 26 14 25 20 10 16 18 34 77 138 5 1 6 0 2 4 1 5 4
Endoplasmic Reticulum 5 27 0 0 0 0 0 1 2 2 3 10 2 1 2 4 2 1 3 1 2 3 8
Endosome 16 2 14 39 39 6 53 6 14 13 8 42 46 96 2 1 2 28 19 6 24 35 42
Golgi 7 7 6 0 4 0 0 0 0 0 1 4 2 5 16 18 11 15 19 5 9 18 19
Mitochondria 206 200 39 101 53 121 156 175 210 290 299 163 139 129 7 3 1 181 209 109 205 221 199
Nucleus 0 2 0 1 2 7 9 4 2 6 6 4 4 2 1 1 4 0 0 0 0 1 1
Nuclear Periphery 7 3 0 0 0 0 3 5 1 4 2 1 0 0 0 1 3 2 2 0 2 1 5
Nucleolus 2 1 0 1 0 5 7 3 3 14 6 1 0 1 1 2 0 1 0 0 0 2 3
Peroxisomes 4 1 9 25 14 1 11 7 8 3 2 8 8 61 0 0 1 10 5 4 12 16 29
SpindlePole 3 2 8 16 21 8 21 7 25 20 17 8 18 7 1 1 1 3 1 0 3 6 10
Vac/Vac Membrane 48 30 11 18 32 26 48 26 27 18 17 27 61 60 13 10 9 8 20 1 9 13 18
Unique Cell Count 244 255 87 165 151 154 258 216 250 328 340 264 299 408 48 37 38 264 301 143 288 375 384
Labelled Cell Count 313 325 113 241 197 194 350 273 327 417 405 322 365 506 58 52 42 264 301 143 288 375 384


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.3 5.3 6.8 6.1 7.0 5.1 6.0 5.3 5.4 5.3 5.5 5.6 6.3 6.1 8.0 8.7 8.3 5.2 5.3 5.6
Std Deviation (1e-4) 0.9 0.9 2.4 2.0 5.2 2.4 2.0 1.6 1.4 1.3 1.5 1.1 2.8 1.5 1.7 1.5 1.8 1.7 1.7 2.2
Intensity Change (Log2) -0.16 0.03 -0.43 -0.19 -0.36 -0.35 -0.37 -0.31 -0.28 -0.11 -0.16 0.24 0.35 0.28 -0.39 -0.37 -0.28


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 1.1 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.1 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -0.4 -2.3 -2.4 -2.5 -4.6 -4.5 -4.3 -1.5 1.2 2.6 -1.4 -2.4 -0.5
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 1.4 1.7 -3.3 0.9 -4.2 -3.1 -4.1 0 0 -0.2 1.5 -2.1 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 4.0 5.4 3.3
Mitochondria 2.5 -1.5 5.3 2.5 6.3 7.2 8.9 8.8 2.8 0.3 -2.4 -3.5 -4.0 -4.7
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 1.1 -0.3 0 -2.1 0 0 0 0 0 0 1.1 0 0 0
SpindlePole 0.1 1.1 -1.2 -0.3 0 0.2 -1.0 -1.5 0 -1.0 0 0 0 0
Vacuole -0.4 1.7 0.9 1.3 -0.1 -0.5 -2.3 -2.6 -0.6 1.6 0.5 2.1 2.0 1.5

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.8769 0.861 0.6702 0.585 0.2835 0.8103 2.8328 2.3099 2.6637 2.3587 2.5379 2.5133 4.519 4.6134 4.1172 4.5599 3.6952 4.4764
Actin 0.0251 0.0089 0.008 0.0058 0.0126 0.0029 0.0384 0.0097 0.0218 0.023 0.0053 0.004 0.0344 0.04 0.0258 0.0614 0.0374 0.0047
Bud 0.0014 0.0127 0.0105 0.0019 0.001 0.0045 0.0016 0.0029 0.003 0.0066 0.002 0.0017 0.0021 0.0039 0.0026 0.0021 0.0005 0.0016
Bud Neck 0.002 0.0017 0.0052 0.0127 0.001 0.0013 0.0018 0.0013 0.0021 0.0013 0.0011 0.0023 0.0027 0.0016 0.0032 0.0007 0.0015 0.0011
Bud Periphery 0.0017 0.0106 0.0077 0.0043 0.0026 0.0032 0.0023 0.0043 0.006 0.0107 0.0031 0.0046 0.0025 0.0147 0.0051 0.0067 0.0009 0.0063
Bud Site 0.006 0.0364 0.0106 0.0075 0.0018 0.0009 0.0132 0.0353 0.0118 0.007 0.0038 0.0012 0.0246 0.0501 0.0083 0.0011 0.0026 0.0008
Cell Periphery 0.0008 0.0017 0.0023 0.0008 0.0006 0.0009 0.0008 0.001 0.0013 0.0007 0.0005 0.001 0.0022 0.0065 0.001 0.0004 0.0003 0.0018
Cytoplasm 0.014 0.0109 0.0142 0.012 0.0049 0.0147 0.0173 0.0131 0.017 0.0224 0.0317 0.0116 0.0271 0.0174 0.0224 0.0065 0.0019 0.0144
Cytoplasmic Foci 0.0434 0.0535 0.0475 0.0681 0.0509 0.0433 0.0424 0.0515 0.0411 0.0376 0.0419 0.027 0.0491 0.0439 0.0308 0.0195 0.0349 0.0515
Eisosomes 0.0009 0.0003 0.0005 0.0003 0.0002 0.0003 0.0008 0.0002 0.0004 0.0004 0.0002 0.0002 0.0034 0.0021 0.0009 0.0004 0.0005 0.0004
Endoplasmic Reticulum 0.0031 0.0009 0.0025 0.0009 0.0008 0.0018 0.0052 0.0032 0.0029 0.0252 0.0031 0.0018 0.0119 0.0017 0.0114 0.0011 0.0017 0.0042
Endosome 0.1685 0.0811 0.1151 0.1348 0.0934 0.0726 0.1461 0.1794 0.1205 0.0886 0.2039 0.1059 0.0447 0.1213 0.1138 0.1326 0.0937 0.0965
Golgi 0.0685 0.0868 0.054 0.0704 0.0577 0.0639 0.078 0.0947 0.0736 0.0792 0.0985 0.0661 0.0393 0.0886 0.0758 0.0725 0.0828 0.0599
Lipid Particles 0.0365 0.0154 0.0143 0.0133 0.0075 0.0132 0.0251 0.0125 0.0103 0.0216 0.0361 0.0094 0.0847 0.0043 0.0118 0.0016 0.0504 0.0219
Mitochondria 0.543 0.6104 0.6211 0.565 0.7212 0.7135 0.5383 0.522 0.6309 0.6064 0.4796 0.7315 0.5318 0.5468 0.5854 0.6703 0.6144 0.6831
None 0.0159 0.0196 0.0335 0.0127 0.0005 0.0088 0.0075 0.0063 0.0012 0.0051 0.0058 0.0014 0.0506 0.0242 0.0508 0.0008 0.0005 0.0048
Nuclear Periphery 0.0047 0.0003 0.0031 0.0007 0.0004 0.0009 0.0118 0.0018 0.0022 0.0087 0.0264 0.002 0.0193 0.0003 0.0047 0.0005 0.0015 0.0013
Nucleolus 0.0042 0.001 0.0019 0.0087 0.0002 0.0009 0.0046 0.0009 0.0008 0.0004 0.0022 0.0003 0.0029 0.0006 0.0004 0.0002 0.0001 0.0004
Nucleus 0.0067 0.0009 0.0021 0.0088 0.0002 0.0013 0.0093 0.0013 0.0014 0.0014 0.0069 0.0006 0.0139 0.0006 0.0014 0.0003 0.0002 0.0007
Peroxisomes 0.0235 0.0359 0.0233 0.0346 0.0344 0.0365 0.0122 0.0206 0.0217 0.0324 0.0209 0.0123 0.0331 0.0212 0.0175 0.0086 0.0693 0.0177
Punctate Nuclear 0.0054 0.0003 0.0023 0.0057 0.0002 0.0026 0.0144 0.0004 0.0007 0.0013 0.0027 0.0008 0.0103 0.0002 0.0019 0.0002 0.0006 0.0027
Vacuole 0.016 0.0072 0.0129 0.022 0.004 0.0067 0.0185 0.0266 0.0201 0.0116 0.0115 0.0076 0.0064 0.0071 0.0149 0.0072 0.0015 0.0139
Vacuole Periphery 0.009 0.0037 0.0075 0.0088 0.0037 0.0056 0.0104 0.0113 0.0091 0.0084 0.0128 0.0069 0.0032 0.0028 0.0099 0.0053 0.0027 0.0103

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 55.4342 47.195 46.6943 45.5339 54.7603 43.971 41.3563 37.5748 45.672 47.5288
Translational Efficiency 0.9369 0.9661 0.9639 1.0521 0.6968 0.9674 1.0713 1.0902 0.9854 0.9245

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
177 875 142 2861 1676 2491 1733 2530 1853 3366 1875 5391

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 642.09 781.52 1021.94 1047.80 684.00 822.38 862.79 1095.33 680.00 811.76 874.84 1070.11
Standard Deviation 86.73 117.16 118.10 173.93 80.87 111.47 160.52 167.21 82.37 114.39 163.23 172.45
Intensity Change Log 2 0.283507 0.670463 0.706516 0.265809 0.335013 0.679297 0.274406 0.507179 0.692541

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000892 0.003148 0.012236 0.011234 0.000994 0.000840 0.005231 0.002192 0.000984 0.001440 0.005762 0.006990
Bud Neck 0.042293 0.016739 0.016153 0.001871 0.020601 0.010571 0.007172 0.001990 0.022673 0.012175 0.007852 0.001927
Bud Site 0.032428 0.049807 0.077687 0.059882 0.025160 0.021102 0.113297* 0.057388 0.025854 0.028564 0.110600* 0.058711
Cell Periphery 0.002922 0.000730 0.000433 0.004274 0.000371 0.000366 0.001511 0.000554 0.000614 0.000461 0.001429 0.002528
Cytoplasm 0.089133 0.021942 0.031920 0.014208 0.044098 0.010841 0.026831 0.020054 0.048400 0.013726 0.027217 0.016951
Cytoplasmic Foci 0.165761 0.108415 0.146659 0.012469 0.145648 0.054632 0.055390 0.032690 0.147569 0.068613 0.062302 0.021959
Eisosomes 0.000695 0.000161 0.000175 0.000136 0.000188 0.000083 0.000107 0.000058 0.000236 0.000103 0.000113 0.000100
Endoplasmic Reticulum 0.002431 0.001414 0.006372 0.001933 0.002634 0.000809 0.003085 0.000776 0.002614 0.000966 0.003334 0.001390
Endosome 0.043419 0.121414 0.143087 0.024101 0.083983 0.094204 0.086365 0.050237 0.080108 0.101277 0.090661 0.036367
Golgi 0.054362 0.147518* 0.124344* 0.072306 0.092124 0.121627* 0.102806 0.147943* 0.088517 0.128357* 0.104438 0.107802
Lipid Particles 0.027178 0.004043 0.012665 0.000167 0.012213 0.003960 0.001085 0.000187 0.013642 0.003982 0.001962 0.000176
Mitochondria 0.400959* 0.385375* 0.089097 0.737566* 0.469043* 0.584983* 0.365683* 0.528450* 0.462540* 0.533094* 0.344737* 0.639428*
Mitotic Spindle 0.001762 0.017409 0.083874 0.010879 0.007866 0.012205 0.042851 0.041038 0.007283 0.013558 0.045957 0.025032
None 0.001398 0.000606 0.000379 0.000606 0.001043 0.001081 0.001072 0.000548 0.001077 0.000957 0.001019 0.000578
Nuclear Periphery 0.000837 0.000155 0.001399 0.000040 0.000530 0.000133 0.000329 0.000222 0.000559 0.000139 0.000410 0.000125
Nuclear Periphery Foci 0.000312 0.000412 0.003790 0.000057 0.000406 0.000422 0.000822 0.000305 0.000397 0.000420 0.001047 0.000173
Nucleolus 0.002268 0.002613 0.009539 0.000031 0.001293 0.000695 0.001930 0.000082 0.001386 0.001194 0.002506 0.000055
Nucleus 0.045104 0.007036 0.014765 0.000125 0.007520 0.002272 0.003715 0.000306 0.011110 0.003511 0.004552 0.000210
Peroxisomes 0.049665 0.050342 0.084092 0.005023 0.032835 0.017063 0.021093 0.005199 0.034442 0.025714 0.025864 0.005106
Vacuole 0.021372 0.037086 0.091303 0.017851 0.030468 0.037721 0.089874 0.050873 0.029599 0.037556 0.089982 0.033348
Vacuole Periphery 0.014808 0.023634 0.050032 0.025243 0.020982 0.024390 0.069750 0.058909 0.020393 0.024193 0.068257 0.041043

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.99 -3.84 -8.87 -6.26 0.33 0.59 -5.51 -3.58 -3.90 3.75 -1.66 -6.40 -9.57 -8.58 -1.28
Bud Neck 3.31 3.23 5.43 7.35 4.56 5.65 8.30 12.26 9.10 8.94 5.92 8.95 13.37 11.69 10.08
Bud Site -2.15 -3.62 -3.66 -2.21 1.68 1.83 -18.98 -10.35 -12.44 11.06 -1.22 -19.23 -13.12 -13.01 11.61
Cell Periphery 2.17 2.47 -1.23 -7.40 -8.14 0.08 -4.24 -3.79 -3.67 3.55 1.40 -3.05 -7.10 -8.18 -3.16
Cytoplasm 5.38 3.60 6.14 3.25 2.36 11.62 5.01 7.95 -6.00 3.17 12.00 6.13 11.01 -2.65 5.42
Cytoplasmic Foci 3.96 1.22 11.26 21.04 12.72 20.48 20.00 26.66 10.21 9.74 18.31 19.38 31.79 24.16 18.49
Eisosomes 5.35 5.10 5.61 2.11 1.86 7.96 5.87 10.03 6.48 9.31 8.46 7.68 8.60 0.69 2.06
Endoplasmic Reticulum 1.40 -2.59 0.70 -1.71 3.18 3.39 -0.79 3.68 0.14 6.49 3.41 -1.35 2.58 -2.17 5.42
Endosome -9.78 -7.60 3.12 18.75 10.32 -2.42 -0.52 9.43 13.96 11.59 -5.49 -2.56 14.01 25.01 19.31
Golgi -9.09 -4.12 -2.11 11.12 3.41 -6.10 -2.05 -10.44 -4.91 -8.24 -9.05 -3.20 -4.78 5.31 -0.90
Lipid Particles 3.70 1.98 4.36 6.05 3.63 6.00 9.23 10.10 6.20 5.00 7.16 9.14 10.94 7.91 5.65
Mitochondria 0.54 10.39 -12.15 -27.84* -46.29* -10.95 8.98 -5.42 5.97 -15.35 -7.12 10.68 -19.08 -14.22 -32.95*
Mitotic Spindle -8.43 -6.38 -10.36 3.44 5.66 -3.46 -12.91 -14.69 -12.55 0.50 -5.58 -14.35 -13.49 -8.42 7.42
None 1.12 1.48 1.17 0.21 -2.29 -0.12 -0.02 3.49 1.91 1.91 0.44 0.26 3.41 1.84 1.73
Nuclear Periphery 2.34 -1.11 2.76 2.91 3.25 2.63 1.19 1.96 -1.57 1.19 3.02 0.93 3.11 0.46 3.53
Nuclear Periphery Foci -0.84 -2.98 2.82 4.74 3.19 -0.07 -2.81 0.88 0.47 3.53 -0.14 -4.02 2.71 1.41 5.74
Nucleolus -0.39 -1.50 3.30 3.05 2.01 2.16 -1.01 5.18 5.25 3.26 0.46 -1.62 6.02 4.84 3.86
Nucleus 3.43 2.54 4.12 3.85 3.96 4.70 2.86 7.33 3.77 4.95 4.97 3.94 7.84 5.45 6.21
Peroxisomes -0.11 -2.34 6.15 12.00 6.30 6.34 4.24 12.25 8.86 8.75 3.50 3.08 13.78 14.70 10.74
Vacuole -3.51 -6.76 1.02 6.22 7.40 -2.98 -16.96 -8.10 -5.32 10.66 -3.68 -18.21 -2.11 2.09 18.00
Vacuole Periphery -2.77 -3.92 -3.71 -0.76 2.81 -2.31 -16.35 -15.85 -14.67 3.03 -2.81 -16.87 -13.04 -11.22 9.28