Standard name | |
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Description | Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 1 | 0 | 0 | 5 | 3 | 5 | 12 | 3 | 15 | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 2 | 3 | 2 | 4 | 3 | 9 | 14 | 8 | 8 | 17 | 6 | 21 | 4 | 1 | 7 | 4 | 2 | 6 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 157 | 185 | 47 | 101 | 159 | 210 | 223 | 237 | 147 | 181 | 122 | 148 | 131 | 162 | 222 | 41 | 97 | 58 | 147 | 176 | 59 | 87 | 142 | 193 |
Endoplasmic Reticulum | 2 | 3 | 1 | 2 | 10 | 12 | 27 | 33 | 23 | 19 | 18 | 25 | 4 | 0 | 1 | 1 | 3 | 3 | 6 | 2 | 4 | 0 | 2 | 4 |
Endosome | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
Golgi | 1 | 0 | 0 | 0 | 1 | 0 | 4 | 3 | 0 | 2 | 0 | 1 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 0 | 1 |
Mitochondria | 2 | 2 | 0 | 2 | 0 | 2 | 15 | 18 | 17 | 23 | 60 | 43 | 0 | 0 | 1 | 2 | 2 | 0 | 1 | 1 | 2 | 0 | 1 | 1 |
Nucleus | 0 | 1 | 1 | 2 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 3 | 0 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 1 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 0 | 1 | 0 | 6 | 2 | 2 | 6 | 11 | 4 | 21 | 7 | 12 | 0 | 1 | 0 | 1 | 9 | 4 | 1 | 6 | 6 | 1 | 1 | 3 |
Unique Cell Count | 162 | 188 | 48 | 111 | 170 | 225 | 273 | 293 | 193 | 248 | 192 | 228 | 133 | 162 | 225 | 47 | 110 | 63 | 158 | 195 | 77 | 92 | 149 | 211 |
Labelled Cell Count | 164 | 195 | 51 | 119 | 178 | 236 | 295 | 313 | 206 | 277 | 216 | 269 | 139 | 164 | 235 | 51 | 116 | 71 | 158 | 195 | 77 | 92 | 149 | 211 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 8.7 | 10.6 | 10.3 | 10.7 | 12.3 | 10.7 | 10.3 | 10.6 | 9.4 | 10.1 | 8.7 | 9.4 | 11.9 | 10.8 | 11.3 | 12.0 | 12.7 | 13.2 | 10.6 | 11.0 | 11.3 |
Std Deviation (1e-4) | 1.1 | 2.1 | 1.6 | 1.8 | 2.1 | 1.7 | 1.9 | 1.8 | 1.6 | 2.0 | 1.7 | 1.8 | 1.7 | 1.6 | 1.5 | 2.3 | 2.8 | 2.6 | 1.5 | 2.0 | 1.8 |
Intensity Change (Log2) | – | – | – | 0.05 | 0.25 | 0.05 | 0.0 | 0.04 | -0.13 | -0.03 | -0.25 | -0.14 | 0.2 | 0.07 | 0.13 | 0.22 | 0.3 | 0.35 | 0.03 | 0.1 | 0.13 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | -1.6 | -1.2 | -1.2 | -2.8 | -2.9 | -3.4 | -3.8 | -4.7 | -4.6 | 0.3 | 1.8 | 0.4 | -2.0 | -2.0 | -1.4 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.5 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |