Standard name
Description Protein involved in G2/M phase progression and response to DNA damage; interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0.05 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0.05 0 0 0.07 0 0.09 0 0 0 0.09 0 0.1 0 0 0 0 0 0
Cytoplasm 0.97 0.98 0.98 0.91 0.94 0.93 0.82 0.81 0.76 0.73 0.64 0.65 0.98 1.0 0.99 0.87 0.88 0.92 0.94 0.9 0.77 0.95 0.96 0.92
Endoplasmic Reticulum 0 0 0 0 0.06 0.05 0.1 0.11 0.12 0.08 0.09 0.11 0 0 0 0 0 0.05 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0.06 0.09 0.09 0.31 0.19 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0.05 0 0 0 0 0 0.08 0 0.05 0 0 0 0 0.08 0.06 0 0 0.08 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 1 0 0 5 3 5 12 3 15 0 0 2 0 0 0 0 1 0 0 0 0
Bud 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 2 3 2 4 3 9 14 8 8 17 6 21 4 1 7 4 2 6 0 0 0 0 0 0
Cytoplasm 157 185 47 101 159 210 223 237 147 181 122 148 131 162 222 41 97 58 147 176 59 87 142 193
Endoplasmic Reticulum 2 3 1 2 10 12 27 33 23 19 18 25 4 0 1 1 3 3 6 2 4 0 2 4
Endosome 0 0 0 0 1 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 1 0 0 1
Golgi 1 0 0 0 1 0 4 3 0 2 0 1 0 0 1 1 1 0 0 1 0 0 0 1
Mitochondria 2 2 0 2 0 2 15 18 17 23 60 43 0 0 1 2 2 0 1 1 2 0 1 1
Nucleus 0 1 1 2 2 1 1 0 0 1 0 3 0 0 1 0 2 0 0 1 0 1 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 1 0 6 2 2 6 11 4 21 7 12 0 1 0 1 9 4 1 6 6 1 1 3
Unique Cell Count 162 188 48 111 170 225 273 293 193 248 192 228 133 162 225 47 110 63 158 195 77 92 149 211
Labelled Cell Count 164 195 51 119 178 236 295 313 206 277 216 269 139 164 235 51 116 71 158 195 77 92 149 211


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.7 10.6 10.3 10.7 12.3 10.7 10.3 10.6 9.4 10.1 8.7 9.4 11.9 10.8 11.3 12.0 12.7 13.2 10.6 11.0 11.3
Std Deviation (1e-4) 1.1 2.1 1.6 1.8 2.1 1.7 1.9 1.8 1.6 2.0 1.7 1.8 1.7 1.6 1.5 2.3 2.8 2.6 1.5 2.0 1.8
Intensity Change (Log2) – – – 0.05 0.25 0.05 0.0 0.04 -0.13 -0.03 -0.25 -0.14 0.2 0.07 0.13 0.22 0.3 0.35 0.03 0.1 0.13


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.6 -1.2 -1.2 -2.8 -2.9 -3.4 -3.8 -4.7 -4.6 0.3 1.8 0.4 -2.0 -2.0 -1.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 4.5 3.3 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0