Standard name | |
---|---|
Description | HDEL receptor; an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | – | 4 | 25 | 18 | 22 | 23 | 42 | 32 | 45 | 62 | 56 | 70 | 106 | 104 | 105 | 30 | 31 | 31 | 1 | 3 | 6 | 21 | 24 | 27 |
Bud | – | 0 | 2 | 1 | 1 | 2 | 8 | 1 | 6 | 6 | 14 | 14 | 12 | 9 | 6 | 10 | 2 | 7 | 0 | 0 | 1 | 0 | 4 | 5 |
Bud Neck | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Bud Site | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | – | 2 | 0 | 1 | 10 | 11 | 7 | 15 | 14 | 18 | 10 | 16 | 5 | 17 | 26 | 3 | 9 | 5 | 0 | 0 | 0 | 0 | 0 | 3 |
Cytoplasm | – | 25 | 5 | 23 | 31 | 38 | 36 | 33 | 33 | 37 | 35 | 29 | 42 | 61 | 64 | 35 | 26 | 29 | 1 | 3 | 1 | 5 | 8 | 14 |
Endoplasmic Reticulum | – | 93 | 111 | 143 | 272 | 228 | 274 | 223 | 234 | 285 | 231 | 319 | 451 | 535 | 550 | 2 | 6 | 2 | 13 | 105 | 113 | 311 | 392 | 565 |
Endosome | – | 0 | 2 | 1 | 1 | 0 | 1 | 2 | 3 | 6 | 3 | 5 | 0 | 0 | 1 | 4 | 5 | 2 | 0 | 0 | 1 | 2 | 6 | 4 |
Golgi | – | 5 | 18 | 26 | 14 | 5 | 20 | 21 | 21 | 40 | 32 | 31 | 119 | 166 | 197 | 87 | 86 | 80 | 0 | 2 | 8 | 33 | 21 | 27 |
Mitochondria | – | 0 | 0 | 1 | 4 | 10 | 12 | 25 | 45 | 43 | 78 | 75 | 0 | 0 | 0 | 14 | 9 | 12 | 0 | 0 | 0 | 2 | 8 | 6 |
Nucleus | – | 0 | 0 | 0 | 0 | 0 | 1 | 4 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | – | 0 | 1 | 0 | 0 | 1 | 0 | 4 | 2 | 5 | 6 | 9 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 1 | 2 | 1 |
Nucleolus | – | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 0 | 4 | 1 | 2 | 0 | 1 | 0 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 0 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | – | 1 | 1 | 2 | 5 | 2 | 22 | 15 | 15 | 53 | 11 | 25 | 13 | 17 | 17 | 16 | 8 | 13 | 0 | 1 | 0 | 4 | 8 | 13 |
Unique Cell Count | 115 | 127 | 176 | 315 | 271 | 351 | 310 | 320 | 419 | 328 | 442 | 553 | 688 | 738 | 168 | 154 | 151 | 18 | 120 | 135 | 388 | 485 | 676 | |
Labelled Cell Count | 130 | 165 | 216 | 360 | 320 | 427 | 378 | 418 | 560 | 477 | 596 | 749 | 914 | 966 | 203 | 186 | 184 | 18 | 120 | 135 | 388 | 485 | 676 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | – | 36.2 | 22.1 | 22.0 | 21.5 | 21.1 | 18.8 | 18.1 | 17.6 | 16.8 | 16.5 | 16.9 | 26.1 | 28.1 | 28.5 | 24.2 | 27.1 | 27.9 | 30.0 | 30.4 | 32.2 |
Std Deviation (1e-4) | – | 6.5 | 4.7 | 4.6 | 4.4 | 3.8 | 3.9 | 4.3 | 4.2 | 3.5 | 3.5 | 4.1 | 5.5 | 6.1 | 6.0 | 8.1 | 8.0 | 9.6 | 5.8 | 7.4 | 7.2 |
Intensity Change (Log2) | – | – | – | -0.01 | -0.04 | -0.07 | -0.23 | -0.29 | -0.33 | -0.39 | -0.42 | -0.39 | 0.24 | 0.35 | 0.37 | 0.13 | 0.29 | 0.34 | 0.44 | 0.46 | 0.54 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.3 | -3.9 | -3.2 | -2.1 | -2.6 | -1.5 | -1.3 | -0.7 | -1.0 | -0.1 | -1.3 | -1.6 | -0.4 | 0.1 | 0.2 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1.9 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2.7 | 2.1 | 3.0 | 2.2 | 2.3 | 2.2 | 1.8 | 2.3 | 1.1 | 1.5 | 1.9 | 1.8 | 4.2 | 3.4 | 3.9 |
Endoplasmic Reticulum | -1.4 | -0.3 | -0.9 | -2.3 | -3.5 | -3.2 | -4.3 | -3.8 | -3.5 | -1.6 | -2.5 | -3.2 | -15.1 | -14.1 | -14.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0.1 | -3.6 | -4.9 | -3.0 | -2.5 | -2.6 | -1.5 | -1.4 | -2.5 | 1.9 | 2.5 | 3.0 | 6.7 | 7.2 | 6.7 |
Mitochondria | 0 | 0 | 0 | 0 | 3.3 | 4.5 | 3.8 | 6.0 | 5.0 | 0 | 0 | 0 | 3.3 | 0 | 3.2 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 2.5 | 0 | 0 | 3.9 | 0 | 2.3 | 0 | 0 | 0 | 3.2 | 0 | 3.0 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
---|
Gene | Images |
---|