Standard name
Description HDEL receptor; an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 0 0.2 0.1 0.07 0.08 0.12 0.1 0.14 0.15 0.17 0.16 0.19 0.15 0.14 0.18 0.2 0.21 0.09 0 0.05 0.05 0.05 0
Bud – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm – 0.22 0 0.13 0.1 0.14 0.1 0.11 0.1 0.09 0.11 0.07 0.08 0.09 0.09 0.21 0.17 0.19 0.06 0 0 0 0 0
Endoplasmic Reticulum – 0.81 0.87 0.81 0.86 0.84 0.78 0.72 0.73 0.68 0.7 0.72 0.82 0.78 0.75 0 0 0 0.77 0.88 0.84 0.8 0.81 0.84
Endosome – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi – 0 0.14 0.15 0 0 0.06 0.07 0.07 0.1 0.1 0.07 0.22 0.24 0.27 0.52 0.56 0.53 0 0 0.06 0.09 0 0
Mitochondria – 0 0 0 0 0 0 0.08 0.14 0.1 0.24 0.17 0 0 0 0.08 0.06 0.08 0.05 0 0 0 0 0
Nucleus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane – 0 0 0 0 0 0.06 0.05 0.05 0.13 0 0.06 0 0 0 0.1 0.05 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches – 4 25 18 22 23 42 32 45 62 56 70 106 104 105 30 31 31 1 3 6 21 24 27
Bud – 0 2 1 1 2 8 1 6 6 14 14 12 9 6 10 2 7 0 0 1 0 4 5
Bud Neck – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1
Bud Site – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery – 2 0 1 10 11 7 15 14 18 10 16 5 17 26 3 9 5 0 0 0 0 0 3
Cytoplasm – 25 5 23 31 38 36 33 33 37 35 29 42 61 64 35 26 29 1 3 1 5 8 14
Endoplasmic Reticulum – 93 111 143 272 228 274 223 234 285 231 319 451 535 550 2 6 2 13 105 113 311 392 565
Endosome – 0 2 1 1 0 1 2 3 6 3 5 0 0 1 4 5 2 0 0 1 2 6 4
Golgi – 5 18 26 14 5 20 21 21 40 32 31 119 166 197 87 86 80 0 2 8 33 21 27
Mitochondria – 0 0 1 4 10 12 25 45 43 78 75 0 0 0 14 9 12 0 0 0 2 8 6
Nucleus – 0 0 0 0 0 1 4 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery – 0 1 0 0 1 0 4 2 5 6 9 0 0 0 0 1 1 0 0 0 1 2 1
Nucleolus – 0 0 0 0 0 4 3 0 4 1 2 0 1 0 2 1 0 0 0 0 0 0 0
Peroxisomes – 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole – 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 1 2 0 0 0 0 0 0
Vac/Vac Membrane – 1 1 2 5 2 22 15 15 53 11 25 13 17 17 16 8 13 0 1 0 4 8 13
Unique Cell Count 115 127 176 315 271 351 310 320 419 328 442 553 688 738 168 154 151 18 120 135 388 485 676
Labelled Cell Count 130 165 216 360 320 427 378 418 560 477 596 749 914 966 203 186 184 18 120 135 388 485 676


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) – 36.2 22.1 22.0 21.5 21.1 18.8 18.1 17.6 16.8 16.5 16.9 26.1 28.1 28.5 24.2 27.1 27.9 30.0 30.4 32.2
Std Deviation (1e-4) – 6.5 4.7 4.6 4.4 3.8 3.9 4.3 4.2 3.5 3.5 4.1 5.5 6.1 6.0 8.1 8.0 9.6 5.8 7.4 7.2
Intensity Change (Log2) – – – -0.01 -0.04 -0.07 -0.23 -0.29 -0.33 -0.39 -0.42 -0.39 0.24 0.35 0.37 0.13 0.29 0.34 0.44 0.46 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.3 -3.9 -3.2 -2.1 -2.6 -1.5 -1.3 -0.7 -1.0 -0.1 -1.3 -1.6 -0.4 0.1 0.2
Bud 0 0 0 0 0 0 0 0 0 0 0 0 1.9 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.7 2.1 3.0 2.2 2.3 2.2 1.8 2.3 1.1 1.5 1.9 1.8 4.2 3.4 3.9
Endoplasmic Reticulum -1.4 -0.3 -0.9 -2.3 -3.5 -3.2 -4.3 -3.8 -3.5 -1.6 -2.5 -3.2 -15.1 -14.1 -14.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.1 -3.6 -4.9 -3.0 -2.5 -2.6 -1.5 -1.4 -2.5 1.9 2.5 3.0 6.7 7.2 6.7
Mitochondria 0 0 0 0 3.3 4.5 3.8 6.0 5.0 0 0 0 3.3 0 3.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.5 0 0 3.9 0 2.3 0 0 0 3.2 0 3.0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available