Standard name
Human Ortholog
Description HDEL receptor; an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0.2 0.1 0.07 0.08 0.12 0.1 0.14 0.15 0.17 0.16 0.19 0.15 0.14 0.18 0.2 0.21 0.09 0 0.05 0.05 0.05 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Cytoplasm 0.22 0 0.13 0.1 0.14 0.1 0.11 0.1 0.09 0.11 0.07 0.08 0.09 0.09 0.21 0.17 0.19 0.06 0 0 0 0 0
Endoplasmic Reticulum 0.81 0.87 0.81 0.86 0.84 0.78 0.72 0.73 0.68 0.7 0.72 0.82 0.78 0.75 0 0 0 0.77 0.88 0.84 0.8 0.81 0.84
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0.14 0.15 0 0 0.06 0.07 0.07 0.1 0.1 0.07 0.22 0.24 0.27 0.52 0.56 0.53 0 0 0.06 0.09 0 0
Mitochondria 0 0 0 0 0 0 0.08 0.14 0.1 0.24 0.17 0 0 0 0.08 0.06 0.08 0.05 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.06 0.05 0.05 0.13 0 0.06 0 0 0 0.1 0.05 0.09 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 4 25 18 22 23 42 32 45 62 56 70 106 104 105 30 31 31 1 3 6 21 24 27
Bud 0 2 1 1 2 8 1 6 6 14 14 12 9 6 10 2 7 0 0 1 0 4 5
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2 0 1 10 11 7 15 14 18 10 16 5 17 26 3 9 5 0 0 0 0 0 3
Cytoplasm 25 5 23 31 38 36 33 33 37 35 29 42 61 64 35 26 29 1 3 1 5 8 14
Endoplasmic Reticulum 93 111 143 272 228 274 223 234 285 231 319 451 535 550 2 6 2 13 105 113 311 392 565
Endosome 0 2 1 1 0 1 2 3 6 3 5 0 0 1 4 5 2 0 0 1 2 6 4
Golgi 5 18 26 14 5 20 21 21 40 32 31 119 166 197 87 86 80 0 2 8 33 21 27
Mitochondria 0 0 1 4 10 12 25 45 43 78 75 0 0 0 14 9 12 0 0 0 2 8 6
Nucleus 0 0 0 0 0 1 4 0 1 0 1 0 0 0 0 1 0 0 0 0 0 0 0
Nuclear Periphery 0 1 0 0 1 0 4 2 5 6 9 0 0 0 0 1 1 0 0 0 1 2 1
Nucleolus 0 0 0 0 0 4 3 0 4 1 2 0 1 0 2 1 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 1 3 0 0 1 2 0 0 0 0 0 0
Vac/Vac Membrane 1 1 2 5 2 22 15 15 53 11 25 13 17 17 16 8 13 0 1 0 4 8 13
Unique Cell Count 115 127 176 315 271 351 310 320 419 328 442 553 688 738 168 154 151 18 120 135 388 485 676
Labelled Cell Count 130 165 216 360 320 427 378 418 560 477 596 749 914 966 203 186 184 18 120 135 388 485 676


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 36.2 22.1 22.0 21.5 21.1 18.8 18.1 17.6 16.8 16.5 16.9 26.1 28.1 28.5 24.2 27.1 27.9 30.0 30.4 32.2
Std Deviation (1e-4) 6.5 4.7 4.6 4.4 3.8 3.9 4.3 4.2 3.5 3.5 4.1 5.5 6.1 6.0 8.1 8.0 9.6 5.8 7.4 7.2
Intensity Change (Log2) -0.01 -0.04 -0.07 -0.23 -0.29 -0.33 -0.39 -0.42 -0.39 0.24 0.35 0.37 0.13 0.29 0.34 0.44 0.46 0.54


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.3 -3.9 -3.2 -2.1 -2.6 -1.5 -1.3 -0.7 -1.0 -0.1 -1.3 -1.6 -0.4 0.1 0.2
Bud 0 0 0 0 0 0 0 0 0 0 0 0 1.9 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.7 2.1 3.0 2.2 2.3 2.2 1.8 2.3 1.1 1.5 1.9 1.8 4.2 3.4 3.9
Endoplasmic Reticulum -1.4 -0.3 -0.9 -2.3 -3.5 -3.2 -4.3 -3.8 -3.5 -1.6 -2.5 -3.2 -15.1 -14.1 -14.6
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0.1 -3.6 -4.9 -3.0 -2.5 -2.6 -1.5 -1.4 -2.5 1.9 2.5 3.0 6.7 7.2 6.7
Mitochondria 0 0 0 0 3.3 4.5 3.8 6.0 5.0 0 0 0 3.3 0 3.2
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 2.5 0 0 3.9 0 2.3 0 0 0 3.2 0 3.0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 17.8117 20.8255 17.6644 19.2864 16.8281 18.9095 16.6082 19.4561 19.7597 16.7384 17.9602 20.0909 19.1375 23.7088 21.847 21.3437 11.3155 23.2393
Actin 0.0143 0.0255 0.0229 0.001 0.0039 0.0084 0.0188 0.0245 0.0337 0.011 0.003 0.012 0.0266 0.0069 0.0151 0.0039 0.1783 0.0103
Bud 0.0001 0.0023 0.0004 0 0.0002 0.0009 0.0004 0.0004 0.0004 0.0001 0.0001 0.0003 0.0006 0 0.0002 0 0.0012 0.0001
Bud Neck 0.0016 0.001 0.001 0.0001 0.0004 0.0014 0.0005 0.0007 0.0008 0.0004 0.0005 0.0027 0.0011 0.0004 0.0015 0.0009 0.0012 0.0011
Bud Periphery 0.0002 0.0022 0.0009 0 0.0003 0.0006 0.0004 0.0003 0.0005 0.0001 0.0004 0.0003 0.0014 0 0.0002 0.0001 0.0016 0.0001
Bud Site 0.0008 0.0127 0.0011 0 0.0008 0.0002 0.0017 0.0122 0.0006 0.0003 0 0.0009 0.0009 0.0001 0.0037 0 0.0039 0
Cell Periphery 0.0023 0.0059 0.003 0.0008 0.0016 0.001 0.0018 0.0022 0.0022 0.0009 0.0008 0.0008 0.0016 0.0031 0.0022 0.0018 0.0017 0.0009
Cytoplasm 0.0267 0.0773 0.0341 0.0061 0.0068 0.0248 0.0362 0.0395 0.0249 0.0125 0.0131 0.0311 0.0224 0.0356 0.0195 0.0098 0.008 0.0148
Cytoplasmic Foci 0.0207 0.0182 0.0211 0.0054 0.0228 0.015 0.0446 0.0439 0.0129 0.0178 0.0072 0.0135 0.0189 0.025 0.0212 0.0106 0.0299 0.0184
Eisosomes 0.0009 0.0008 0.0003 0 0.0002 0.0003 0.0005 0.0004 0.0002 0.0001 0.0002 0.0005 0.0004 0.0022 0.0003 0.0008 0.0024 0.0001
Endoplasmic Reticulum 0.66 0.6258 0.6757 0.8637 0.7172 0.66 0.5109 0.5754 0.7123 0.685 0.7316 0.6484 0.4925 0.567 0.5613 0.7172 0.3707 0.4518
Endosome 0.0626 0.0456 0.0649 0.0276 0.0499 0.0632 0.0789 0.0616 0.0487 0.0616 0.0771 0.064 0.061 0.052 0.0571 0.0607 0.08 0.0776
Golgi 0.0894 0.076 0.0978 0.0511 0.1058 0.1678 0.1648 0.1581 0.1065 0.1703 0.1339 0.1787 0.2513 0.1953 0.2383 0.1306 0.152 0.3264
Lipid Particles 0.0517 0.0907 0.0404 0.0366 0.0489 0.0232 0.0792 0.0565 0.0185 0.0247 0.009 0.0166 0.051 0.1019 0.0582 0.0359 0.1024 0.064
Mitochondria 0.0042 0.0019 0.0128 0.0006 0.0155 0.0035 0.0136 0.0017 0.0044 0.0013 0.0038 0.0065 0.0024 0.0005 0.0015 0.0009 0.0268 0.0013
None 0.0133 0.0004 0.0016 0.0001 0.0005 0.0005 0.0027 0.0076 0.0006 0.0022 0.001 0.0058 0.011 0.0002 0.0009 0.0005 0.0029 0.0002
Nuclear Periphery 0.028 0.0057 0.0082 0.0042 0.0128 0.0164 0.0216 0.0077 0.0216 0.0043 0.0098 0.0113 0.034 0.0067 0.0102 0.0203 0.015 0.0273
Nucleolus 0.0018 0.0001 0.0002 0 0.0001 0.0003 0.001 0.0001 0.0001 0.0001 0 0.0003 0.0006 0 0.0002 0 0.0003 0
Nucleus 0.0028 0.0007 0.0006 0.0001 0.0003 0.0035 0.0037 0.0005 0.0014 0.0002 0.0003 0.0011 0.0033 0.0002 0.0011 0.0004 0.0009 0.0004
Peroxisomes 0.0031 0.0004 0.0038 0.0001 0.006 0.0007 0.0036 0.001 0.0005 0.0002 0.0001 0.0004 0.0026 0.0002 0.0014 0.0001 0.0084 0.0002
Punctate Nuclear 0.0065 0.0005 0.0017 0.0001 0.0007 0.0016 0.0083 0.001 0.0024 0.0005 0.0002 0.0005 0.0077 0.0002 0.0011 0.0002 0.0015 0.0003
Vacuole 0.0054 0.0053 0.0052 0.0013 0.0025 0.0038 0.0036 0.0033 0.0036 0.0047 0.0047 0.0026 0.005 0.0015 0.003 0.0026 0.0074 0.0017
Vacuole Periphery 0.0035 0.0011 0.0024 0.0009 0.0027 0.003 0.0034 0.0013 0.0033 0.0017 0.0033 0.0019 0.0036 0.0009 0.0019 0.0025 0.0034 0.0029

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 66.873 31.3847 45.4686 55.6486 42.2681 43.4434 49.8662 58.1336 46.3165 73.2392
Translational Efficiency 1.3839 1.9865 1.2104 1.0812 1.743 2.1375 1.4207 1.1178 1.4347 1.1894

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description HDEL receptor; an integral membrane protein that binds to the HDEL motif in proteins destined for retention in the endoplasmic reticulum; has a role in maintenance of normal levels of ER-resident proteins
Localization
Cell Percentages ER (98%)
Cell Cycle Regulation No
Subcompartmental Group ER-5

Erd2

Erd2


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Erd2-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available