Standard name
Human Ortholog
Description Alpha-adaptin; large subunit of the clathrin associated protein complex (AP-2); involved in vesicle mediated transport

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.64 0.51 0.55 0.52 0.4 0.47 0.36 0.33 0.35 0.34 0.28 0.73 0.7 0.71 0.4 0.4 0.49 0.42 0.43 0.53 0.29 0.45 0.45
Bud 0.14 0.14 0.22 0.22 0.27 0.23 0.23 0.21 0.16 0.2 0.15 0.18 0.17 0.18 0.07 0.06 0.11 0.06 0.07 0 0.21 0.12 0.11
Bud Neck 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0.2 0.26 0.2 0.36 0.22 0.22
Bud Site 0.23 0.23 0.3 0.34 0.21 0.2 0.1 0.09 0.09 0.07 0.07 0.13 0.08 0.07 0.1 0.1 0.09
Cell Periphery 0.21 0.16 0.12 0.09 0.09 0.11 0.14 0.12 0.2 0.18 0.15 0.27 0.3 0.36 0.2 0.21 0.2 0.23 0.11 0.12 0.06 0.15 0.15
Cytoplasm 0 0.08 0.08 0 0.06 0.06 0.06 0.08 0.06 0.06 0.07 0.05 0.06 0.12 0.09 0.1 0.1 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0.17 0.05 0 0 0 0 0 0
Mitochondria 0.12 0.25 0.26 0.53 0.3 0.52 0.56 0.56 0.72 0.7 0.72 0.15 0.09 0.18 0.11 0.13 0.15 0 0 0 0 0 0
Nucleus 0.05 0.05 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0.07 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0.27 0.15 0.14 0.26 0.22 0.19 0.16 0.13 0.13 0.13 0.14 0.24 0.26 0.11 0.23 0.25 0.21 0 0 0 0 0 0
SpindlePole 0.08 0.06 0.07 0 0.05 0 0 0 0 0 0 0.07 0.06 0 0.12 0.09 0.1 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 125 145 275 192 157 265 194 209 173 131 127 228 305 336 137 62 118 99 157 317 67 176 240
Bud 28 40 108 83 105 129 121 130 80 79 68 56 74 85 25 9 27 14 25 22 47 48 59
Bud Neck 5 3 21 11 25 15 6 11 0 2 0 4 2 0 6 5 2 47 95 123 85 88 116
Bud Site 45 64 149 126 81 112 56 56 43 28 33 39 35 33 34 15 22
Cell Periphery 41 45 59 35 35 64 74 76 101 70 68 85 132 169 70 32 48 54 38 69 13 58 80
Cytoplasm 4 23 38 15 25 32 34 50 28 25 31 15 25 56 31 16 25 0 2 2 1 0 1
Endoplasmic Reticulum 1 6 12 5 11 14 34 28 32 14 19 5 6 17 14 5 9 1 6 8 1 5 5
Endosome 1 1 0 2 2 1 3 3 0 0 0 0 0 0 3 1 3 1 5 9 3 1 3
Golgi 5 0 0 0 0 0 0 0 0 0 0 0 0 0 31 27 12 2 3 0 0 3 3
Mitochondria 24 70 131 195 117 293 301 355 358 275 321 46 37 85 37 20 35 8 13 22 6 5 7
Nucleus 9 15 16 19 19 12 17 16 7 6 1 0 2 2 23 6 10 0 0 0 0 0 0
Nuclear Periphery 0 2 1 2 1 5 3 4 8 1 2 0 0 1 0 0 0 0 0 3 0 0 2
Nucleolus 0 0 0 2 9 5 4 10 3 5 4 0 0 0 0 2 0 0 0 0 0 0 0
Peroxisomes 52 41 70 95 86 106 83 79 64 51 63 76 114 50 77 39 49 1 0 8 1 3 2
SpindlePole 15 17 33 6 21 10 12 22 3 5 6 22 25 10 41 14 25 0 0 1 0 0 0
Vac/Vac Membrane 0 5 4 2 3 4 7 19 2 5 7 0 0 1 8 3 1 1 9 7 2 0 3
Unique Cell Count 195 282 497 370 389 559 535 630 495 391 447 311 433 470 342 156 239 235 364 601 234 395 530
Labelled Cell Count 355 477 917 790 697 1067 949 1068 902 697 750 576 757 845 537 256 386 235 364 601 234 395 530


Bud Neck, Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.6 6.5 5.1 5.2 5.5 5.2 5.1 5.3 4.6 4.7 4.6 6.3 6.7 5.9 8.9 9.5 8.8 7.2 7.3 7.4
Std Deviation (1e-4) 1.5 1.1 0.8 1.0 1.2 1.2 1.2 1.2 1.0 0.9 1.1 1.2 1.1 1.0 1.7 1.9 2.1 1.6 1.6 1.7
Intensity Change (Log2) 0.03 0.09 0.03 -0.01 0.05 -0.17 -0.14 -0.17 0.3 0.38 0.2 0.79 0.88 0.78 0.48 0.5 0.52


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -1.0 -4.4 -2.6 -6.1 -7.5 0 0 0 5.1 4.7 5.2 -4.3 -3.4 -1.5
Bud 0.2 1.8 0.5 0.3 -0.4 0 0 0 -1.3 -1.8 -1.4 -5.6 -4.5 -3.4
Bud Neck -1.0 1.5 -1.4 -3.1 -2.5 0 0 0 -2.3 -3.7 -4.5 -2.0 -0.6 -2.5
Bud Site 1.3 -3.1 -3.7 -7.9 -9.1 0 0 0 -5.7 -8.4 -9.1 -6.9 -5.1 -6.2
Cell Periphery -1.1 -1.4 -0.2 0.9 0.1 0 0 0 5.6 7.0 8.8 3.4 2.7 3.0
Cytoplasm -2.2 -0.7 -1.3 -0.8 0.2 -1.3 -0.7 -0.4 -1.6 -1.1 2.2 0.7 1.0 1.3
Endoplasmic Reticulum -1.1 0.4 0.1 3.1 1.8 0 0 0 -0.8 -1.1 1.1 1.4 0 1.0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 6.8 9.5 0
Mitochondria 7.9 1.2 8.6 9.7 10.1 0 0 0 -3.9 -7.0 -3.1 -5.5 -3.5 -3.6
Nucleus 1.4 1.3 -1.1 0 -0.7 -1.9 -1.6 -3.5 -3.2 -3.0 -3.2 2.4 0.4 0.7
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 4.3 3.1 2.1 0.6 -0.8 0 0 0 3.7 4.7 -1.6 3.2 3.2 2.2
SpindlePole -3.5 -0.8 -4.0 -3.5 -2.4 0 0 0 0.2 -0.5 -3.4 2.7 1.0 1.8
Vacuole 0 0 0 0.8 2.6 0 0 1.1 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 5.3314 4.7211 5.0189 4.3214 4.7299 5.0626 3.2336 2.8076 3.3927 2.6024 2.5077 3.2023 8.2628 7.5522 8.1536 8.1412 7.4309 8.4179
Actin 0.2509 0.1288 0.1401 0.1568 0.2936 0.1606 0.1862 0.1608 0.1125 0.151 0.213 0.1999 0.2156 0.1248 0.1426 0.2424 0.3253 0.2064
Bud 0.007 0.0038 0.0038 0.0032 0.0069 0.0061 0.0111 0.0045 0.0051 0.0053 0.0084 0.0093 0.0039 0.0031 0.0024 0.0024 0.0039 0.0034
Bud Neck 0.1264 0.0286 0.4034 0.5809 0.2225 0.6087 0.1176 0.036 0.4865 0.5814 0.3855 0.4838 0.1592 0.0475 0.5226 0.5652 0.2389 0.5376
Bud Periphery 0.029 0.0078 0.0182 0.0294 0.0584 0.0624 0.0451 0.0106 0.0315 0.0398 0.0735 0.0916 0.0206 0.0072 0.0134 0.0301 0.0258 0.0362
Bud Site 0.2451 0.7723 0.3002 0.0555 0.0636 0.0146 0.3628 0.7132 0.2903 0.0562 0.0276 0.0371 0.2949 0.7439 0.2554 0.0303 0.069 0.0281
Cell Periphery 0.1381 0.0202 0.0426 0.0845 0.2493 0.0839 0.1637 0.0281 0.0517 0.12 0.168 0.1183 0.1428 0.0466 0.0332 0.0951 0.1414 0.1309
Cytoplasm 0.0197 0.0084 0.0057 0.0038 0.0065 0.0071 0.0133 0.0129 0.0019 0.0117 0.0051 0.0076 0.0076 0.0115 0.0027 0.0021 0.0041 0.0081
Cytoplasmic Foci 0.0132 0.0024 0.0064 0.0018 0.0086 0.0044 0.0026 0.0025 0.0009 0.0011 0.0014 0.0018 0.0064 0.0007 0.0011 0.0011 0.0033 0.0008
Eisosomes 0.0205 0.0038 0.0103 0.0154 0.0299 0.0193 0.0627 0.0179 0.0071 0.0181 0.1002 0.0287 0.1078 0.0123 0.0201 0.0225 0.1612 0.0386
Endoplasmic Reticulum 0.0054 0.0008 0.0037 0.0074 0.0015 0.0009 0.0021 0.0006 0.0007 0.0014 0.0013 0.0018 0.0015 0.0002 0.0006 0.0006 0.0013 0.0005
Endosome 0.0297 0.0046 0.0151 0.0074 0.0037 0.0031 0.0011 0.0005 0.0003 0.0007 0.0006 0.001 0.0046 0.0001 0.0004 0.0002 0.0028 0.0002
Golgi 0.0169 0.0021 0.0069 0.0142 0.0055 0.0049 0.0024 0.0017 0.001 0.0023 0.0027 0.0028 0.0034 0.0003 0.001 0.0016 0.007 0.001
Lipid Particles 0.0178 0.0013 0.0062 0.0047 0.0032 0.0022 0.004 0.0005 0.0006 0.0017 0.001 0.0008 0.0036 0.0001 0.0006 0.0004 0.0049 0.0003
Mitochondria 0.0317 0.0065 0.0073 0.0248 0.0292 0.007 0.0088 0.0031 0.0037 0.0016 0.0027 0.0063 0.0046 0.0001 0.0003 0.0011 0.0024 0.0006
None 0.0067 0.0004 0.0005 0.0005 0.0008 0.0049 0.0022 0.0011 0.0002 0.0008 0.0009 0.0014 0.0031 0.0003 0.0004 0.0004 0.0009 0.0034
Nuclear Periphery 0.0093 0.0007 0.0069 0.0019 0.0009 0.0002 0.0005 0.0001 0.0002 0.0004 0.0004 0.0003 0.0026 0.0001 0.0001 0.0002 0.0005 0.0001
Nucleolus 0.0016 0.0008 0.0008 0.0005 0.0008 0.0002 0.0015 0.0003 0.0009 0.001 0.0013 0.0011 0.0005 0.0002 0.0008 0.0003 0.0003 0.0003
Nucleus 0.0118 0.003 0.0115 0.0007 0.0011 0.0003 0.0012 0.0003 0.0007 0.001 0.0009 0.0009 0.0008 0.0002 0.0006 0.0004 0.0003 0.0003
Peroxisomes 0.0081 0.0007 0.0055 0.0012 0.0058 0.0075 0.0052 0.0044 0.0012 0.0008 0.0012 0.0011 0.0132 0.0005 0.0006 0.0022 0.0052 0.0005
Punctate Nuclear 0.0051 0.0001 0.0006 0.0002 0.0004 0.0001 0.0005 0.0002 0.0001 0.0001 0.0001 0.0001 0.0014 0.0001 0.0004 0.0001 0.0006 0.0001
Vacuole 0.0046 0.0019 0.0033 0.0032 0.0056 0.0012 0.0049 0.0007 0.0027 0.0033 0.0039 0.0038 0.0016 0.0004 0.0008 0.0011 0.0008 0.0024
Vacuole Periphery 0.0014 0.0009 0.001 0.0021 0.0021 0.0003 0.0003 0.0001 0.0002 0.0003 0.0003 0.0005 0.0003 0 0.0001 0.0001 0.0002 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 13.9808 14.1002 11.4142 18.6153 13.4788 12.5399 19.36 21.6503 17.2373 19.7447
Translational Efficiency 0.8726 0.8959 0.8264 0.5213 0.7229 0.8736 0.7513 0.4471 0.664 0.5735

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1616 1422 285 938 1303 1429 755 128 2919 2851 1040 1066

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 740.19 820.84 1165.76 956.17 747.48 843.32 1019.12 1050.96 743.44 832.11 1059.31 967.55
Standard Deviation 90.18 133.79 137.65 132.91 111.33 117.09 130.41 155.09 100.24 126.20 147.71 139.22
Intensity Change Log 2 0.149205 0.655303 0.369371 0.174045 0.447217 0.491601 0.161740 0.554500 0.432080

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.094331 0.209676* 0.254794* 0.200868* 0.216526* 0.126987* 0.189791* 0.202960* 0.148877* 0.168230* 0.207604* 0.201119*
Bud Neck 0.228186* 0.164708 0.222736* 0.083355 0.134396 0.221252* 0.227264* 0.097285 0.186319* 0.193049* 0.226023* 0.085027
Bud Site 0.174387* 0.251575* 0.271585* 0.436348* 0.127439* 0.197485* 0.351987* 0.342653* 0.153430* 0.224464* 0.329953* 0.425097*
Cell Periphery 0.399966* 0.209394* 0.145817* 0.198555* 0.320280* 0.380557* 0.164475* 0.213933* 0.364395* 0.295185* 0.159362* 0.200401*
Cytoplasm 0.030315 0.035308 0.011442 0.016475 0.033453 0.019733 0.012800 0.013635 0.031716 0.027502 0.012428 0.016134
Cytoplasmic Foci 0.007189 0.018967 0.009526 0.003099 0.011984 0.004927 0.004823 0.006614 0.009330 0.011930 0.006112 0.003521
Eisosomes 0.038082 0.021779 0.030957 0.026953 0.062565 0.022007 0.020131 0.046139 0.049011 0.021893 0.023097 0.029257
Endoplasmic Reticulum 0.001621 0.005283 0.011130 0.004434 0.004700 0.001147 0.007686 0.005070 0.002995 0.003210 0.008629 0.004511
Endosome 0.001923 0.013001 0.003428 0.003339 0.005505 0.003614 0.001870 0.006921 0.003522 0.008296 0.002297 0.003769
Golgi 0.002811 0.017231 0.001500 0.002510 0.011964 0.004082 0.002251 0.001580 0.006897 0.010640 0.002045 0.002398
Lipid Particles 0.001262 0.006692 0.003835 0.000558 0.007776 0.000708 0.001387 0.007628 0.004170 0.003693 0.002058 0.001407
Mitochondria 0.008418 0.017459 0.001462 0.008427 0.016564 0.010714 0.001394 0.001506 0.012055 0.014078 0.001413 0.007596
Mitotic Spindle 0.000257 0.000370 0.000278 0.002154 0.000246 0.000459 0.000219 0.000235 0.000252 0.000415 0.000235 0.001924
None 0.000508 0.000762 0.000033 0.000054 0.000436 0.000116 0.000036 0.000019 0.000476 0.000438 0.000036 0.000050
Nuclear Periphery 0.000026 0.000026 0.000052 0.000033 0.000033 0.000024 0.000023 0.000141 0.000029 0.000025 0.000031 0.000046
Nuclear Periphery Foci 0.000086 0.000220 0.001317 0.000187 0.000127 0.000100 0.000470 0.000341 0.000104 0.000160 0.000702 0.000206
Nucleolus 0.000025 0.000037 0.000024 0.000006 0.000028 0.000038 0.000011 0.000011 0.000027 0.000037 0.000014 0.000006
Nucleus 0.001464 0.001112 0.002839 0.001688 0.000841 0.000550 0.002095 0.001221 0.001186 0.000830 0.002299 0.001632
Peroxisomes 0.008107 0.022426 0.002846 0.001328 0.043876 0.003930 0.001888 0.014933 0.024074 0.013156 0.002150 0.002962
Vacuole 0.000900 0.002675 0.024345 0.008763 0.000877 0.001299 0.009285 0.032391 0.000890 0.001985 0.013412 0.011601
Vacuole Periphery 0.000135 0.001299 0.000053 0.000867 0.000382 0.000272 0.000115 0.004782 0.000245 0.000784 0.000098 0.001337

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -13.58 -9.05 -11.22 0.84 2.73 9.74* 2.26 0.63 -3.05 -0.46 -3.08 -6.14 -5.77 -3.55 0.51
Bud Neck 7.06 0.53 17.92 10.83 8.29 -9.61 -8.30 2.37 7.53 7.31 -1.03 -4.08 15.98 16.99 15.16
Bud Site -8.34 -5.72 -21.22 -13.97 -8.79 -8.40 -18.30 -7.07 -4.70 0.39 -11.30 -17.66 -24.52 -17.51 -6.95
Cell Periphery 17.38 17.01 17.29 1.05 -3.58 -4.91 13.13 4.19 6.51 -1.84 8.23 22.64 16.58 9.71 -3.93
Cytoplasm -1.51 4.09 4.51 5.93 -1.06 4.73 6.58 4.02 1.11 -0.32 1.90 7.71 6.32 4.65 -1.44
Cytoplasmic Foci -6.00 -1.12 3.16 8.35 2.40 4.31 3.58 1.33 -0.73 -0.60 -2.06 1.75 4.95 6.55 2.02
Eisosomes 5.88 1.69 3.45 -1.88 0.80 12.03 12.35 1.99 -2.92 -3.15 12.58 10.60 6.84 -2.96 -2.28
Endoplasmic Reticulum -4.98 -5.28 -2.32 0.56 3.20 4.09 -1.98 -0.18 -2.17 1.24 -0.41 -5.07 -1.35 -1.19 2.79
Endosome -8.50 -2.21 -1.65 6.39 0.32 1.83 4.79 -0.41 -0.93 -1.43 -5.78 2.35 -0.29 4.02 -1.53
Golgi -8.49 2.06 0.27 7.78 -0.96 5.06 5.97 6.10 1.82 0.47 -3.32 4.99 4.01 6.44 -0.30
Lipid Particles -5.41 -2.24 2.06 6.22 2.81 6.31 5.54 0.01 -1.45 -1.30 0.64 3.03 3.44 2.89 0.99
Mitochondria -5.17 5.56 0.01 3.71 -3.23 2.70 9.57 8.69 5.00 -0.09 -1.48 11.28 2.44 3.25 -3.52
Mitotic Spindle -2.14 -0.80 -2.77 -2.59 -2.70 -0.95 0.64 0.13 0.93 -0.15 -1.43 0.28 -2.78 -2.46 -2.79
None -0.58 2.37 2.28 1.77 -1.24 2.65 3.29 3.46 7.56 2.10 0.15 3.57 3.46 1.95 -0.88
Nuclear Periphery 0.00 -2.79 -0.79 -0.72 1.96 0.78 1.96 -0.94 -1.01 -1.02 0.62 -1.57 -1.09 -1.25 -0.55
Nuclear Periphery Foci -3.81 -4.77 -2.70 0.56 4.39 0.80 -3.35 -2.18 -2.36 0.85 -2.38 -5.83 -2.96 -1.24 4.67
Nucleolus -2.11 -0.68 5.71 7.16 3.21 -0.48 3.85 2.11 1.26 0.06 -1.02 2.57 7.64 3.02 3.46
Nucleus 0.83 -1.97 -0.58 -1.03 1.42 2.87 -2.80 -1.00 -1.45 1.10 1.51 -2.94 -0.98 -1.48 1.07
Peroxisomes -6.99 4.75 6.77 11.31 2.23 13.12 13.89 3.35 -1.36 -1.61 6.21 14.54 11.75 7.20 -0.74
Vacuole -4.05 -4.67 -5.52 -4.34 3.16 -1.30 -4.84 -3.15 -3.12 -2.32 -4.00 -6.64 -6.08 -5.52 0.88
Vacuole Periphery -2.09 2.98 -2.29 0.68 -2.53 0.79 1.95 -0.93 -0.96 -0.99 -1.89 2.00 -1.73 -0.80 -1.96