Standard name | |
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Description | B subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 2 | 0 | 2 | 8 | 5 | – | 8 | 6 | 0 | 0 | 0 | 0 | 0 | 0 | 4 | 3 | 0 | 0 | 0 | 2 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Site | 0 | 0 | 0 | 1 | 3 | 8 | 6 | 5 | 13 | – | 5 | 12 | 0 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 9 | 7 | 8 | 10 | 16 | 11 | 20 | 18 | 8 | – | 8 | 15 | 3 | 18 | 28 | 49 | 9 | 23 | 0 | 0 | 0 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 0 | 0 | 3 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 13 | 7 | 9 | 0 | 0 | 0 | 0 | 1 | 2 |
Endosome | 1 | 1 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 2 | 0 | 3 | 0 | 0 | 3 |
Golgi | 0 | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 5 | 1 | 0 | 3 | 0 | 0 | 1 |
Mitochondria | 9 | 12 | 3 | 15 | 9 | 67 | 89 | 52 | 195 | – | 244 | 134 | 5 | 1 | 4 | 6 | 2 | 5 | 2 | 2 | 7 | 0 | 1 | 3 |
Nucleus | 223 | 304 | 410 | 297 | 423 | 438 | 625 | 528 | 481 | – | 434 | 617 | 112 | 145 | 188 | 287 | 199 | 240 | 208 | 295 | 379 | 28 | 107 | 98 |
Nuclear Periphery | 17 | 19 | 9 | 13 | 35 | 25 | 44 | 24 | 24 | – | 17 | 22 | 5 | 1 | 3 | 29 | 17 | 25 | 18 | 8 | 18 | 1 | 0 | 1 |
Nucleolus | 21 | 22 | 5 | 8 | 6 | 22 | 26 | 19 | 33 | – | 57 | 53 | 8 | 11 | 17 | 13 | 7 | 3 | 13 | 10 | 12 | 0 | 2 | 6 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 1 | 0 | 3 | 1 | 4 | 5 | 3 | 9 | 2 | – | 1 | 6 | 0 | 0 | 3 | 3 | 1 | 1 | 2 | 1 | 1 | 0 | 0 | 2 |
Vac/Vac Membrane | 17 | 21 | 25 | 7 | 31 | 19 | 38 | 20 | 16 | – | 18 | 22 | 1 | 2 | 2 | 105 | 58 | 68 | 12 | 19 | 21 | 2 | 28 | 30 |
Unique Cell Count | 259 | 335 | 441 | 318 | 471 | 501 | 738 | 610 | 572 | 583 | 738 | 120 | 168 | 227 | 458 | 273 | 341 | 268 | 346 | 453 | 38 | 148 | 160 | |
Labelled Cell Count | 298 | 387 | 469 | 352 | 529 | 596 | 857 | 683 | 778 | 792 | 887 | 134 | 178 | 246 | 513 | 304 | 381 | 268 | 346 | 453 | 38 | 148 | 160 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 6.2 | 7.1 | 6.6 | 5.9 | 6.5 | 5.2 | 5.6 | 5.9 | 4.7 | – | 4.5 | 5.2 | 6.6 | 7.8 | 6.6 | 9.6 | 9.5 | 9.2 | – | 5.8 | 6.1 |
Std Deviation (1e-4) | 1.1 | 1.7 | 1.0 | 1.1 | 1.1 | 1.0 | 1.2 | 1.1 | 1.0 | – | 1.0 | 0.9 | 1.5 | 8.7 | 1.9 | 2.2 | 2.0 | 2.2 | – | 1.3 | 1.4 |
Intensity Change (Log2) | – | – | – | -0.16 | -0.02 | -0.34 | -0.25 | -0.16 | -0.5 | – | -0.55 | -0.34 | 0.01 | 0.25 | -0.01 | 0.54 | 0.53 | 0.49 | – | -0.19 | -0.12 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 1.2 | 1.5 | 0.4 | 1.0 | 1.2 | -0.5 | – | -0.6 | 0.3 | 0 | 4.9 | 5.7 | 5.5 | 1.3 | 3.5 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 3.2 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 3.6 | 1.6 | 7.4 | 7.0 | 5.6 | 0 | – | 0 | 9.1 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.2 | -1.7 | -2.8 | -4.2 | -3.3 | -4.3 | – | -7.7 | -4.6 | 0.1 | -2.6 | -4.1 | -10.9 | -7.4 | -8.4 |
Nuclear Periphery | 1.7 | 3.8 | 2.4 | 3.1 | 1.7 | 0 | – | 0 | 1.0 | 0 | 0 | 0 | 3.2 | 2.9 | 3.6 |
Nucleolus | 1.4 | 0.2 | 3.0 | 2.5 | 2.1 | 0 | – | 0 | 4.6 | 0 | 0 | 4.4 | 1.8 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 1.2 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | -2.3 | 0.6 | -1.4 | -0.4 | -1.9 | -2.3 | – | -2.0 | -2.3 | -2.2 | -2.4 | -3.0 | 7.4 | 6.3 | 6.1 |