Standard name
Description B subunit of DNA polymerase alpha-primase complex; required for initiation of DNA replication during mitotic and premeiotic DNA synthesis; also functions in telomere capping and length regulation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 0 – 0 0 0 0.11 0.12 0.11 0 0.07 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0.05 0 0.13 0.12 0.09 0.34 – 0.42 0.18 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.86 0.91 0.93 0.93 0.9 0.87 0.85 0.87 0.84 – 0.74 0.84 0.93 0.86 0.83 0.63 0.73 0.7 0.78 0.85 0.84 0.74 0.73 0.62
Nuclear Periphery 0.07 0.06 0 0 0.07 0.05 0.06 0 0 – 0 0 0 0 0 0.06 0.06 0.07 0.07 0 0 0 0 0
Nucleolus 0.08 0.07 0 0 0 0 0 0 0.06 – 0.1 0.07 0.07 0.07 0.07 0 0 0 0.05 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.07 0.06 0.06 0 0.07 0 0.05 0 0 – 0 0 0 0 0 0.23 0.21 0.2 0.05 0.06 0.05 0.07 0.19 0.19
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 1 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 1 1 1 0 0 0
Bud 0 0 0 0 2 0 2 8 5 – 8 6 0 0 0 0 0 0 4 3 0 0 0 2
Bud Neck 0 0 0 0 0 0 1 0 1 – 0 0 0 0 0 0 0 0 0 0 0 0 0 1
Bud Site 0 0 0 1 3 8 6 5 13 – 5 12 0 0 1 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 2 2 0 0 0 0 0 0 0
Cytoplasm 9 7 8 10 16 11 20 18 8 – 8 15 3 18 28 49 9 23 0 0 0 0 0 0
Endoplasmic Reticulum 0 1 1 0 0 0 3 0 0 – 0 0 0 0 0 13 7 9 0 0 0 0 1 2
Endosome 1 1 2 0 0 1 0 0 0 – 0 0 0 0 0 3 1 2 2 0 3 0 0 3
Golgi 0 0 2 0 0 0 0 0 0 – 0 0 0 0 0 2 1 5 1 0 3 0 0 1
Mitochondria 9 12 3 15 9 67 89 52 195 – 244 134 5 1 4 6 2 5 2 2 7 0 1 3
Nucleus 223 304 410 297 423 438 625 528 481 – 434 617 112 145 188 287 199 240 208 295 379 28 107 98
Nuclear Periphery 17 19 9 13 35 25 44 24 24 – 17 22 5 1 3 29 17 25 18 8 18 1 0 1
Nucleolus 21 22 5 8 6 22 26 19 33 – 57 53 8 11 17 13 7 3 13 10 12 0 2 6
Peroxisomes 0 0 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0
SpindlePole 1 0 3 1 4 5 3 9 2 – 1 6 0 0 3 3 1 1 2 1 1 0 0 2
Vac/Vac Membrane 17 21 25 7 31 19 38 20 16 – 18 22 1 2 2 105 58 68 12 19 21 2 28 30
Unique Cell Count 259 335 441 318 471 501 738 610 572 583 738 120 168 227 458 273 341 268 346 453 38 148 160
Labelled Cell Count 298 387 469 352 529 596 857 683 778 792 887 134 178 246 513 304 381 268 346 453 38 148 160


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.2 7.1 6.6 5.9 6.5 5.2 5.6 5.9 4.7 – 4.5 5.2 6.6 7.8 6.6 9.6 9.5 9.2 – 5.8 6.1
Std Deviation (1e-4) 1.1 1.7 1.0 1.1 1.1 1.0 1.2 1.1 1.0 – 1.0 0.9 1.5 8.7 1.9 2.2 2.0 2.2 – 1.3 1.4
Intensity Change (Log2) – – – -0.16 -0.02 -0.34 -0.25 -0.16 -0.5 – -0.55 -0.34 0.01 0.25 -0.01 0.54 0.53 0.49 – -0.19 -0.12


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Cytoplasm 1.2 1.5 0.4 1.0 1.2 -0.5 – -0.6 0.3 0 4.9 5.7 5.5 1.3 3.5
Endoplasmic Reticulum 0 0 0 0 0 0 – 0 0 0 0 0 3.2 0 0
Endosome 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
Mitochondria 3.6 1.6 7.4 7.0 5.6 0 – 0 9.1 0 0 0 0 0 0
Nucleus 0.2 -1.7 -2.8 -4.2 -3.3 -4.3 – -7.7 -4.6 0.1 -2.6 -4.1 -10.9 -7.4 -8.4
Nuclear Periphery 1.7 3.8 2.4 3.1 1.7 0 – 0 1.0 0 0 0 3.2 2.9 3.6
Nucleolus 1.4 0.2 3.0 2.5 2.1 0 – 0 4.6 0 0 4.4 1.8 0 0
Peroxisomes 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 1.2 0 – 0 0 0 0 0 0 0 0
Vacuole -2.3 0.6 -1.4 -0.4 -1.9 -2.3 – -2.0 -2.3 -2.2 -2.4 -3.0 7.4 6.3 6.1