Standard name
Description Component of the mitotic spindle; binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.97 0.98 0.98 0.94 0.9 0.87 0.83 0.75 0.71 1.0 0.99 0.98 0.89 0.93 0.75 0.82 0.69 0.9 0.85 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.08 0 0.14 0.21 0.23 0.35 0.42 0.41 0 0 0 0.06 0 0.12 0.07 0.17 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 3 2 0 2 5
Bud 1 1 2 1 0 3 3 14 8 6 2 0 1 2 0 1 0 5 0 0 8 16
Bud Neck 1 0 2 1 3 2 11 13 2 0 1 0 0 0 1 0 0 3 2 1 0 2
Bud Site 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0
Cell Periphery 3 0 0 2 3 1 4 5 0 1 1 0 0 1 0 0 0 1 3 1 1 2
Cytoplasm 156 326 194 198 348 321 448 617 274 114 103 206 476 487 87 75 131 281 128 205 274 304
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 0 0 1 0 1 2 3 3 1 0 2 0 0 1
Endosome 2 2 0 0 0 0 2 2 0 0 0 0 1 0 1 1 3 2 2 1 1 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0
Mitochondria 6 12 12 16 15 49 105 164 116 64 60 1 3 14 6 0 21 24 30 8 13 18
Nucleus 0 0 0 0 0 1 3 1 2 3 1 0 0 1 0 0 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 1 0 0 2 2 0 0 0 0 1 0 1 0 0 0 1
Nucleolus 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 0 1 0 0 0 0 2 0 0 0 0 0 0 0
SpindlePole 0 1 1 0 2 1 1 21 3 3 3 0 0 0 2 1 3 1 2 0 2 5
Vac/Vac Membrane 3 0 0 1 0 2 2 3 0 2 1 0 0 2 2 1 3 7 5 2 7 8
Unique Cell Count 161 336 199 202 354 343 500 708 331 153 146 207 479 496 98 81 175 344 187 228 321 382
Labelled Cell Count 174 345 211 219 371 382 583 841 405 197 177 207 482 509 105 84 175 344 187 228 321 382


Ambiguous, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.3 3.1 2.9 3.3 2.9 2.8 2.9 2.5 2.8 2.5 4.2 3.9 3.6 4.6 5.5 5.4 3.9 3.7 4.3
Std Deviation (1e-4) 0.8 1.1 1.2 1.4 1.6 1.2 1.4 1.4 1.2 1.8 1.6 1.7 1.3 1.7 1.4 2.5 2.1 1.1 1.2 1.5
Intensity Change (Log2) -0.1 0.08 -0.11 -0.13 -0.1 -0.3 -0.15 -0.29 0.43 0.34 0.2 0.57 0.82 0.79 0.32 0.25 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Stu1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available