Standard name
Human Ortholog
Description Component of the mitotic spindle; binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.97 0.97 0.97 0.98 0.98 0.94 0.9 0.87 0.83 0.75 0.71 1.0 0.99 0.98 0.89 0.93 0.75 0.82 0.69 0.9 0.85 0.8
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.08 0 0.14 0.21 0.23 0.35 0.42 0.41 0 0 0 0.06 0 0.12 0.07 0.17 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 1 3 2 0 2 5
Bud 1 1 2 1 0 3 3 14 8 6 2 0 1 2 0 1 0 5 0 0 8 16
Bud Neck 1 0 2 1 3 2 11 13 2 0 1 0 0 0 1 0 0 3 2 1 0 2
Bud Site 0 0 0 0 0 1 0 0 0 1 1 0 0 0 0 0
Cell Periphery 3 0 0 2 3 1 4 5 0 1 1 0 0 1 0 0 0 1 3 1 1 2
Cytoplasm 156 326 194 198 348 321 448 617 274 114 103 206 476 487 87 75 131 281 128 205 274 304
Endoplasmic Reticulum 0 2 0 0 0 0 0 0 0 0 1 0 1 2 3 3 1 0 2 0 0 1
Endosome 2 2 0 0 0 0 2 2 0 0 0 0 1 0 1 1 3 2 2 1 1 4
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 2 0 0 0 0 0
Mitochondria 6 12 12 16 15 49 105 164 116 64 60 1 3 14 6 0 21 24 30 8 13 18
Nucleus 0 0 0 0 0 1 3 1 2 3 1 0 0 1 0 0 0 1 0 0 0 0
Nuclear Periphery 0 1 0 0 0 0 1 0 0 2 2 0 0 0 0 1 0 1 0 0 0 1
Nucleolus 0 0 0 0 0 0 2 0 0 0 1 0 0 0 0 1 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 1 0 1 0 0 0 0 2 0 0 0 0 0 0 0
SpindlePole 0 1 1 0 2 1 1 21 3 3 3 0 0 0 2 1 3 1 2 0 2 5
Vac/Vac Membrane 3 0 0 1 0 2 2 3 0 2 1 0 0 2 2 1 3 7 5 2 7 8
Unique Cell Count 161 336 199 202 354 343 500 708 331 153 146 207 479 496 98 81 175 344 187 228 321 382
Labelled Cell Count 174 345 211 219 371 382 583 841 405 197 177 207 482 509 105 84 175 344 187 228 321 382


Ambiguous, SpindlePole

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 4.1 3.3 3.1 2.9 3.3 2.9 2.8 2.9 2.5 2.8 2.5 4.2 3.9 3.6 4.6 5.5 5.4 3.9 3.7 4.3
Std Deviation (1e-4) 0.8 1.1 1.2 1.4 1.6 1.2 1.4 1.4 1.2 1.8 1.6 1.7 1.3 1.7 1.4 2.5 2.1 1.1 1.2 1.5
Intensity Change (Log2) -0.1 0.08 -0.11 -0.13 -0.1 -0.3 -0.15 -0.29 0.43 0.34 0.2 0.57 0.82 0.79 0.32 0.25 0.47


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.6149 1.3237 0.9341 0.4939 0.8417 1.0797 0.0671 0.3929 0.5799 -0.025 0.0664 0.3414 -0.1619 -0.2567 -0.2901 -0.1079 -0.2323 -0.2701
Actin 0.0445 0.0003 0.0136 0.0003 0.0079 0.0084 0.0095 0.0002 0.0022 0.0001 0.0327 0.0004 0.0088 0.0018 0.0142 0.0009 0.0005 0.0104
Bud 0.0018 0.001 0.0017 0.0001 0.0016 0.0004 0.0004 0.0016 0.0003 0 0.0006 0.0006 0.0014 0.0002 0.0019 0.0001 0 0.0002
Bud Neck 0.0195 0.0005 0.0028 0.0007 0.001 0.007 0.0005 0.0022 0.0015 0.0005 0.0098 0.0115 0.0011 0.0024 0.0013 0.0005 0.0002 0.0007
Bud Periphery 0.005 0.0031 0.0049 0.0002 0.0034 0.0035 0.0008 0.0048 0.0009 0 0.0007 0.0007 0.0017 0.0002 0.0025 0.0001 0 0.0003
Bud Site 0.0191 0.0079 0.013 0.0001 0.0089 0.0032 0.0029 0.0056 0.0102 0 0.0104 0.0004 0.0161 0.036 0.003 0.0001 0 0.0001
Cell Periphery 0.0021 0.0006 0.0011 0.0001 0.0013 0.0006 0.0003 0.0003 0.0001 0 0.0002 0.0001 0.0003 0.0003 0.0007 0 0 0
Cytoplasm 0.0473 0.0689 0.0892 0.0771 0.0753 0.1358 0.0388 0.0747 0.0487 0.047 0.1241 0.1794 0.0328 0.0697 0.0315 0.0439 0.0323 0.0757
Cytoplasmic Foci 0.0457 0.0198 0.0454 0.0737 0.0951 0.0337 0.0622 0.0262 0.0974 0.0309 0.031 0.0159 0.0362 0.0587 0.0722 0.0539 0.0222 0.0309
Eisosomes 0.0019 0.0002 0.0008 0.0001 0.0012 0.0003 0.0003 0.0001 0.0001 0 0.0002 0 0.0008 0.0004 0.0005 0.0003 0.0001 0.0002
Endoplasmic Reticulum 0.0156 0.0009 0.0022 0.0005 0.0014 0.0018 0.0186 0.0006 0.0007 0.0003 0.0107 0.001 0.0051 0.0005 0.0018 0.0007 0.0002 0.0006
Endosome 0.0327 0.0025 0.0069 0.0027 0.0347 0.0041 0.0309 0.0036 0.0045 0.0093 0.0197 0.0029 0.0302 0.0033 0.0076 0.011 0.0004 0.0019
Golgi 0.0125 0.0001 0.0045 0.0002 0.0076 0.002 0.0125 0.0006 0.0005 0.0004 0.0044 0.0003 0.0198 0.0007 0.0023 0.0141 0.0001 0.0012
Lipid Particles 0.0207 0.0008 0.0042 0.0005 0.0141 0.0021 0.0291 0.0063 0.0018 0.0131 0.0015 0.0008 0.0083 0.0011 0.008 0.0011 0.0003 0.0008
Mitochondria 0.0184 0.0003 0.0147 0.0002 0.0566 0.0034 0.0072 0.0031 0.0009 0.0001 0.0009 0.0012 0.0025 0.0314 0.0041 0.0064 0 0.0006
None 0.6386 0.8745 0.7289 0.701 0.674 0.7619 0.6943 0.8386 0.7646 0.8063 0.6718 0.7584 0.8144 0.7321 0.7831 0.7834 0.9016 0.8599
Nuclear Periphery 0.0168 0.0011 0.0019 0.0007 0.0007 0.0007 0.0071 0.001 0.0009 0.0054 0.0044 0.003 0.0013 0.0021 0.0027 0.0003 0.0002 0.0003
Nucleolus 0.0028 0.0013 0.002 0.0005 0.0009 0.0004 0.0009 0.0003 0.0006 0.0007 0.0004 0.0002 0.0011 0.001 0.0017 0.0002 0.0001 0.0002
Nucleus 0.0051 0.0051 0.0025 0.0034 0.001 0.0018 0.0319 0.0028 0.0083 0.0062 0.0069 0.0084 0.0019 0.0079 0.0032 0.0008 0.0003 0.0011
Peroxisomes 0.0151 0.0003 0.0029 0.0008 0.0034 0.0079 0.0118 0.0036 0.004 0.0009 0.0017 0.0105 0.0027 0.0037 0.0051 0.003 0.0017 0.0023
Punctate Nuclear 0.0187 0.0095 0.0532 0.1369 0.0017 0.0202 0.0333 0.0225 0.0509 0.0782 0.0635 0.0037 0.0122 0.046 0.0506 0.0788 0.0396 0.0124
Vacuole 0.0125 0.0012 0.0031 0.0002 0.0063 0.0006 0.0053 0.001 0.0008 0.0004 0.0034 0.0007 0.0012 0.0004 0.0017 0.0003 0.0001 0.0002
Vacuole Periphery 0.0036 0.0001 0.0006 0 0.002 0.0002 0.0015 0.0002 0.0001 0.0001 0.0009 0.0001 0.0004 0.0001 0.0002 0.0002 0 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 20.4064 8.9039 5.9625 11.3056 16.6936 17.2387 15.4803 12.1524 9.7086 19.4379
Translational Efficiency 0.9529 1.1771 0.8887 0.6523 0.713 1.023 0.5745 0.5697 0.7615 0.6835

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency