Standard name | |
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Human Ortholog | |
Description | Component of the mitotic spindle; binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 3 | 2 | 0 | 2 | 5 |
Bud | 1 | 1 | 2 | 1 | 0 | 3 | 3 | 14 | 8 | – | 6 | 2 | 0 | 1 | 2 | – | 0 | 1 | 0 | 5 | 0 | 0 | 8 | 16 |
Bud Neck | 1 | 0 | 2 | 1 | 3 | 2 | 11 | 13 | 2 | – | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 3 | 2 | 1 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 0 | 2 | 3 | 1 | 4 | 5 | 0 | – | 1 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 2 |
Cytoplasm | 156 | 326 | 194 | 198 | 348 | 321 | 448 | 617 | 274 | – | 114 | 103 | 206 | 476 | 487 | – | 87 | 75 | 131 | 281 | 128 | 205 | 274 | 304 |
Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 2 | – | 3 | 3 | 1 | 0 | 2 | 0 | 0 | 1 |
Endosome | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | – | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 3 | 2 | 2 | 1 | 1 | 4 |
Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 6 | 12 | 12 | 16 | 15 | 49 | 105 | 164 | 116 | – | 64 | 60 | 1 | 3 | 14 | – | 6 | 0 | 21 | 24 | 30 | 8 | 13 | 18 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | – | 3 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 2 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 21 | 3 | – | 3 | 3 | 0 | 0 | 0 | – | 2 | 1 | 3 | 1 | 2 | 0 | 2 | 5 |
Vac/Vac Membrane | 3 | 0 | 0 | 1 | 0 | 2 | 2 | 3 | 0 | – | 2 | 1 | 0 | 0 | 2 | – | 2 | 1 | 3 | 7 | 5 | 2 | 7 | 8 |
Unique Cell Count | 161 | 336 | 199 | 202 | 354 | 343 | 500 | 708 | 331 | 153 | 146 | 207 | 479 | 496 | 98 | 81 | 175 | 344 | 187 | 228 | 321 | 382 | ||
Labelled Cell Count | 174 | 345 | 211 | 219 | 371 | 382 | 583 | 841 | 405 | 197 | 177 | 207 | 482 | 509 | 105 | 84 | 175 | 344 | 187 | 228 | 321 | 382 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 4.1 | 3.3 | 3.1 | 2.9 | 3.3 | 2.9 | 2.8 | 2.9 | 2.5 | – | 2.8 | 2.5 | 4.2 | 3.9 | 3.6 | 4.6 | 5.5 | 5.4 | 3.9 | 3.7 | 4.3 |
Std Deviation (1e-4) | 0.8 | 1.1 | 1.2 | 1.4 | 1.6 | 1.2 | 1.4 | 1.4 | 1.2 | – | 1.8 | 1.6 | 1.7 | 1.3 | 1.7 | 1.4 | 2.5 | 2.1 | 1.1 | 1.2 | 1.5 |
Intensity Change (Log2) | – | – | – | -0.1 | 0.08 | -0.11 | -0.13 | -0.1 | -0.3 | – | -0.15 | -0.29 | 0.43 | 0.34 | 0.2 | 0.57 | 0.82 | 0.79 | 0.32 | 0.25 | 0.47 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 1.6149 | 1.3237 | 0.9341 | 0.4939 | 0.8417 | 1.0797 | 0.0671 | 0.3929 | 0.5799 | -0.025 | 0.0664 | 0.3414 | -0.1619 | -0.2567 | -0.2901 | -0.1079 | -0.2323 | -0.2701 |
Actin | 0.0445 | 0.0003 | 0.0136 | 0.0003 | 0.0079 | 0.0084 | 0.0095 | 0.0002 | 0.0022 | 0.0001 | 0.0327 | 0.0004 | 0.0088 | 0.0018 | 0.0142 | 0.0009 | 0.0005 | 0.0104 |
Bud | 0.0018 | 0.001 | 0.0017 | 0.0001 | 0.0016 | 0.0004 | 0.0004 | 0.0016 | 0.0003 | 0 | 0.0006 | 0.0006 | 0.0014 | 0.0002 | 0.0019 | 0.0001 | 0 | 0.0002 |
Bud Neck | 0.0195 | 0.0005 | 0.0028 | 0.0007 | 0.001 | 0.007 | 0.0005 | 0.0022 | 0.0015 | 0.0005 | 0.0098 | 0.0115 | 0.0011 | 0.0024 | 0.0013 | 0.0005 | 0.0002 | 0.0007 |
Bud Periphery | 0.005 | 0.0031 | 0.0049 | 0.0002 | 0.0034 | 0.0035 | 0.0008 | 0.0048 | 0.0009 | 0 | 0.0007 | 0.0007 | 0.0017 | 0.0002 | 0.0025 | 0.0001 | 0 | 0.0003 |
Bud Site | 0.0191 | 0.0079 | 0.013 | 0.0001 | 0.0089 | 0.0032 | 0.0029 | 0.0056 | 0.0102 | 0 | 0.0104 | 0.0004 | 0.0161 | 0.036 | 0.003 | 0.0001 | 0 | 0.0001 |
Cell Periphery | 0.0021 | 0.0006 | 0.0011 | 0.0001 | 0.0013 | 0.0006 | 0.0003 | 0.0003 | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0.0003 | 0.0007 | 0 | 0 | 0 |
Cytoplasm | 0.0473 | 0.0689 | 0.0892 | 0.0771 | 0.0753 | 0.1358 | 0.0388 | 0.0747 | 0.0487 | 0.047 | 0.1241 | 0.1794 | 0.0328 | 0.0697 | 0.0315 | 0.0439 | 0.0323 | 0.0757 |
Cytoplasmic Foci | 0.0457 | 0.0198 | 0.0454 | 0.0737 | 0.0951 | 0.0337 | 0.0622 | 0.0262 | 0.0974 | 0.0309 | 0.031 | 0.0159 | 0.0362 | 0.0587 | 0.0722 | 0.0539 | 0.0222 | 0.0309 |
Eisosomes | 0.0019 | 0.0002 | 0.0008 | 0.0001 | 0.0012 | 0.0003 | 0.0003 | 0.0001 | 0.0001 | 0 | 0.0002 | 0 | 0.0008 | 0.0004 | 0.0005 | 0.0003 | 0.0001 | 0.0002 |
Endoplasmic Reticulum | 0.0156 | 0.0009 | 0.0022 | 0.0005 | 0.0014 | 0.0018 | 0.0186 | 0.0006 | 0.0007 | 0.0003 | 0.0107 | 0.001 | 0.0051 | 0.0005 | 0.0018 | 0.0007 | 0.0002 | 0.0006 |
Endosome | 0.0327 | 0.0025 | 0.0069 | 0.0027 | 0.0347 | 0.0041 | 0.0309 | 0.0036 | 0.0045 | 0.0093 | 0.0197 | 0.0029 | 0.0302 | 0.0033 | 0.0076 | 0.011 | 0.0004 | 0.0019 |
Golgi | 0.0125 | 0.0001 | 0.0045 | 0.0002 | 0.0076 | 0.002 | 0.0125 | 0.0006 | 0.0005 | 0.0004 | 0.0044 | 0.0003 | 0.0198 | 0.0007 | 0.0023 | 0.0141 | 0.0001 | 0.0012 |
Lipid Particles | 0.0207 | 0.0008 | 0.0042 | 0.0005 | 0.0141 | 0.0021 | 0.0291 | 0.0063 | 0.0018 | 0.0131 | 0.0015 | 0.0008 | 0.0083 | 0.0011 | 0.008 | 0.0011 | 0.0003 | 0.0008 |
Mitochondria | 0.0184 | 0.0003 | 0.0147 | 0.0002 | 0.0566 | 0.0034 | 0.0072 | 0.0031 | 0.0009 | 0.0001 | 0.0009 | 0.0012 | 0.0025 | 0.0314 | 0.0041 | 0.0064 | 0 | 0.0006 |
None | 0.6386 | 0.8745 | 0.7289 | 0.701 | 0.674 | 0.7619 | 0.6943 | 0.8386 | 0.7646 | 0.8063 | 0.6718 | 0.7584 | 0.8144 | 0.7321 | 0.7831 | 0.7834 | 0.9016 | 0.8599 |
Nuclear Periphery | 0.0168 | 0.0011 | 0.0019 | 0.0007 | 0.0007 | 0.0007 | 0.0071 | 0.001 | 0.0009 | 0.0054 | 0.0044 | 0.003 | 0.0013 | 0.0021 | 0.0027 | 0.0003 | 0.0002 | 0.0003 |
Nucleolus | 0.0028 | 0.0013 | 0.002 | 0.0005 | 0.0009 | 0.0004 | 0.0009 | 0.0003 | 0.0006 | 0.0007 | 0.0004 | 0.0002 | 0.0011 | 0.001 | 0.0017 | 0.0002 | 0.0001 | 0.0002 |
Nucleus | 0.0051 | 0.0051 | 0.0025 | 0.0034 | 0.001 | 0.0018 | 0.0319 | 0.0028 | 0.0083 | 0.0062 | 0.0069 | 0.0084 | 0.0019 | 0.0079 | 0.0032 | 0.0008 | 0.0003 | 0.0011 |
Peroxisomes | 0.0151 | 0.0003 | 0.0029 | 0.0008 | 0.0034 | 0.0079 | 0.0118 | 0.0036 | 0.004 | 0.0009 | 0.0017 | 0.0105 | 0.0027 | 0.0037 | 0.0051 | 0.003 | 0.0017 | 0.0023 |
Punctate Nuclear | 0.0187 | 0.0095 | 0.0532 | 0.1369 | 0.0017 | 0.0202 | 0.0333 | 0.0225 | 0.0509 | 0.0782 | 0.0635 | 0.0037 | 0.0122 | 0.046 | 0.0506 | 0.0788 | 0.0396 | 0.0124 |
Vacuole | 0.0125 | 0.0012 | 0.0031 | 0.0002 | 0.0063 | 0.0006 | 0.0053 | 0.001 | 0.0008 | 0.0004 | 0.0034 | 0.0007 | 0.0012 | 0.0004 | 0.0017 | 0.0003 | 0.0001 | 0.0002 |
Vacuole Periphery | 0.0036 | 0.0001 | 0.0006 | 0 | 0.002 | 0.0002 | 0.0015 | 0.0002 | 0.0001 | 0.0001 | 0.0009 | 0.0001 | 0.0004 | 0.0001 | 0.0002 | 0.0002 | 0 | 0.0001 |
R1 | R2 | |||||||||
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G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 20.4064 | 8.9039 | 5.9625 | 11.3056 | 16.6936 | 17.2387 | 15.4803 | 12.1524 | 9.7086 | 19.4379 |
Translational Efficiency | 0.9529 | 1.1771 | 0.8887 | 0.6523 | 0.713 | 1.023 | 0.5745 | 0.5697 | 0.7615 | 0.6835 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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