| Standard name | |
|---|---|
| Description | Component of the mitotic spindle; binds to interpolar microtubules via its association with beta-tubulin (Tub2p); required for interpolar microtubules to provide an outward force on the spindle poles |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 1 | 3 | 2 | 0 | 2 | 5 |
| Bud | 1 | 1 | 2 | 1 | 0 | 3 | 3 | 14 | 8 | – | 6 | 2 | 0 | 1 | 2 | – | 0 | 1 | 0 | 5 | 0 | 0 | 8 | 16 |
| Bud Neck | 1 | 0 | 2 | 1 | 3 | 2 | 11 | 13 | 2 | – | 0 | 1 | 0 | 0 | 0 | – | 1 | 0 | 0 | 3 | 2 | 1 | 0 | 2 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | – | 1 | 1 | 0 | 0 | 0 | – | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 3 | 0 | 0 | 2 | 3 | 1 | 4 | 5 | 0 | – | 1 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 3 | 1 | 1 | 2 |
| Cytoplasm | 156 | 326 | 194 | 198 | 348 | 321 | 448 | 617 | 274 | – | 114 | 103 | 206 | 476 | 487 | – | 87 | 75 | 131 | 281 | 128 | 205 | 274 | 304 |
| Endoplasmic Reticulum | 0 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 2 | – | 3 | 3 | 1 | 0 | 2 | 0 | 0 | 1 |
| Endosome | 2 | 2 | 0 | 0 | 0 | 0 | 2 | 2 | 0 | – | 0 | 0 | 0 | 1 | 0 | – | 1 | 1 | 3 | 2 | 2 | 1 | 1 | 4 |
| Golgi | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 2 | 0 | 0 | 0 | 0 | 0 |
| Mitochondria | 6 | 12 | 12 | 16 | 15 | 49 | 105 | 164 | 116 | – | 64 | 60 | 1 | 3 | 14 | – | 6 | 0 | 21 | 24 | 30 | 8 | 13 | 18 |
| Nucleus | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 1 | 2 | – | 3 | 1 | 0 | 0 | 1 | – | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 2 | 2 | 0 | 0 | 0 | – | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 1 |
| Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | – | 1 | 0 | 0 | 0 | 0 | – | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 1 | 1 | 0 | 2 | 1 | 1 | 21 | 3 | – | 3 | 3 | 0 | 0 | 0 | – | 2 | 1 | 3 | 1 | 2 | 0 | 2 | 5 |
| Vac/Vac Membrane | 3 | 0 | 0 | 1 | 0 | 2 | 2 | 3 | 0 | – | 2 | 1 | 0 | 0 | 2 | – | 2 | 1 | 3 | 7 | 5 | 2 | 7 | 8 |
| Unique Cell Count | 161 | 336 | 199 | 202 | 354 | 343 | 500 | 708 | 331 | 153 | 146 | 207 | 479 | 496 | 98 | 81 | 175 | 344 | 187 | 228 | 321 | 382 | ||
| Labelled Cell Count | 174 | 345 | 211 | 219 | 371 | 382 | 583 | 841 | 405 | 197 | 177 | 207 | 482 | 509 | 105 | 84 | 175 | 344 | 187 | 228 | 321 | 382 | ||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 4.1 | 3.3 | 3.1 | 2.9 | 3.3 | 2.9 | 2.8 | 2.9 | 2.5 | – | 2.8 | 2.5 | 4.2 | 3.9 | 3.6 | 4.6 | 5.5 | 5.4 | 3.9 | 3.7 | 4.3 |
| Std Deviation (1e-4) | 0.8 | 1.1 | 1.2 | 1.4 | 1.6 | 1.2 | 1.4 | 1.4 | 1.2 | – | 1.8 | 1.6 | 1.7 | 1.3 | 1.7 | 1.4 | 2.5 | 2.1 | 1.1 | 1.2 | 1.5 |
| Intensity Change (Log2) | – | – | – | -0.1 | 0.08 | -0.11 | -0.13 | -0.1 | -0.3 | – | -0.15 | -0.29 | 0.43 | 0.34 | 0.2 | 0.57 | 0.82 | 0.79 | 0.32 | 0.25 | 0.47 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Stu1-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
|---|
Images are not yet available
Images are not yet available
