Standard name
Human Ortholog
Description Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0.09 0.06 0.07 0 0 0.06 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.43 0.45 0.55 0.54 0.49 0.52 0.4 0.41 0.35 0.44 0.26 0.36 0.6 0.62 0.67 0.15 0.17 0.18 0.26 0.2 0.23 0.29 0.31 0.35
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.07 0.24 0 0.06 0.12 0.06 0 0 0 0 0 0 0.13 0.19 0.23 0.41 0.4 0.44 0.06 0.12 0.07 0.09 0.09 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.38 0.08 0 0.07 0.06 0.25 0.46 0.33 0.57 0.48 0.73 0.5 0 0 0 0 0.06 0.05 0.12 0.12 0.15 0.12 0.13 0.13
Nucleus 0 0.06 0 0 0 0 0 0 0 0 0.05 0.05 0 0 0 0.06 0 0 0 0.05 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.21 0.24 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0.12 0.08 0.33 0.32 0.32 0.2 0.18 0.25 0.14 0.16 0.12 0.14 0.2 0.19 0.1 0.49 0.49 0.44 0.31 0.27 0.39 0.36 0.3 0.27
Vac/Vac Membrane 0.1 0.17 0 0 0.06 0 0 0 0.05 0 0 0.06 0 0.08 0.07 0.15 0.11 0.11 0.09 0.08 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 2 0 1 0 1 0 0 0 0 0 1 0 0 0 0 0 2 3 1 5 9 16
Bud 1 1 2 7 0 6 12 18 21 18 14 8 1 1 0 0 2 2 2 4 2 2 10 16
Bud Neck 0 1 20 15 23 6 9 26 8 12 4 12 10 6 2 0 0 0 1 2 2 1 4 10
Bud Site 0 0 0 1 0 0 12 6 10 14 14 5 0 0 0 1 4 4 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 1 0 0 0 0 0 0 1 1 1 0 0 0 0 0 1 1
Cytoplasm 68 58 118 129 166 164 154 186 148 206 81 124 122 249 248 52 47 75 55 35 68 86 126 150
Endoplasmic Reticulum 0 4 2 0 1 0 0 0 0 0 0 3 2 1 3 11 12 15 1 0 1 1 2 4
Endosome 12 31 4 14 39 19 12 15 13 9 4 11 27 74 84 143 107 184 11 22 22 25 36 19
Golgi 1 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 1 2 0 2 0 0 1 3
Mitochondria 61 10 1 16 20 79 175 151 242 224 228 175 2 0 0 8 15 23 26 20 43 33 54 56
Nucleus 3 8 4 1 6 12 12 16 12 19 15 16 7 6 3 22 11 12 2 8 3 2 1 0
Nuclear Periphery 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 1 0 0 3 1
Nucleolus 7 4 0 1 1 1 9 2 2 5 5 4 6 0 1 40 56 100 2 5 3 2 2 0
Peroxisomes 0 0 0 2 2 4 1 0 1 0 1 3 1 5 6 3 0 9 1 0 1 0 2 1
SpindlePole 20 10 70 77 108 65 68 114 59 73 36 47 40 77 36 173 132 186 66 47 114 105 125 117
Vac/Vac Membrane 16 22 8 4 19 12 6 20 23 13 11 22 9 31 26 53 31 48 19 14 12 7 12 13
Unique Cell Count 160 129 215 239 339 318 383 459 424 468 313 348 204 399 368 352 270 419 212 179 297 293 413 432
Labelled Cell Count 189 149 231 268 386 368 471 555 539 593 413 430 228 451 410 508 420 661 212 179 297 293 413 432


Microtubule

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.5 4.7 5.0 4.8 4.6 3.9 3.6 4.1 3.7 3.6 3.3 3.7 4.9 5.3 5.4 6.8 6.1 5.8 4.9 5.1 5.7
Std Deviation (1e-4) 0.6 0.9 1.0 2.0 1.2 1.7 1.6 1.1 2.2 1.2 0.8 0.8 1.4 1.4 1.4 1.1 1.2 1.2 1.4 1.8 2.4
Intensity Change (Log2) – – – -0.05 -0.12 -0.35 -0.46 -0.28 -0.44 -0.45 -0.61 -0.44 -0.03 0.08 0.11 0.45 0.29 0.21 -0.03 0.02 0.2


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – 0 0 0
Bud Neck – – – – – – – – – – – – 0 0 0
Bud Site – – – – – – – – – – – – 0 0 0
Cell Periphery – – – – – – – – – – – – 0 0 0
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – 0 0 0
Endosome – – – – – – – – – – – – 0 0 0
Golgi – – – – – – – – – – – – 0 0 0
Mitochondria – – – – – – – – – – – – 0 0 0
Nuclear Periphery – – – – – – – – – – – – 0 0 0
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – 0 0 0
Peroxisomes – – – – – – – – – – – – 0 0 0
SpindlePole – – – – – – – – – – – – 0 0 0
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – 0 0 0
Cytoplasm – – – – – – – – – – – – 0 0 0
Nucleus – – – – – – – – – – – – 0 0 0
Vacuole – – – – – – – – – – – – 4.2 3.1 3.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 0.0891 -0.4304 -0.2063 -0.3544 -0.5975 -0.3538 0.1717 -0.4908 -0.402 -0.1567 -0.5933 -0.085 -0.8354 -0.5923 -0.5295 -0.0675 -0.4599 -0.6536
Actin 0.0243 0.0009 0.0086 0.0347 0.0152 0.0147 0.0212 0.001 0.0038 0.0029 0.0018 0.0015 0.0121 0.0008 0.0097 0.0145 0.0048 0.0039
Bud 0.0007 0.0007 0.0006 0.0117 0.0006 0.0039 0.0016 0.0002 0.0001 0.0004 0.0003 0.0001 0.0006 0.0003 0.0002 0.0007 0.0011 0.0003
Bud Neck 0.0065 0.0015 0.0036 0.0025 0.0013 0.0023 0.02 0.001 0.0014 0.0015 0.0019 0.0039 0.0025 0.0015 0.0017 0.0038 0.0022 0.0041
Bud Periphery 0.0009 0.0008 0.0008 0.0029 0.0016 0.0057 0.0045 0.0003 0.0002 0.0007 0.0006 0.0001 0.0006 0.0003 0.0002 0.0008 0.001 0.0003
Bud Site 0.0032 0.0109 0.0172 0.0029 0.0013 0.0008 0.0168 0.0021 0.0009 0.0017 0.0009 0.0003 0.0023 0.0038 0.0045 0.0076 0.0014 0.0007
Cell Periphery 0.0002 0.0002 0.0008 0.0006 0.0001 0.0001 0.0009 0.0001 0 0 0.0001 0 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001
Cytoplasm 0.0834 0.1375 0.0859 0.0829 0.1078 0.126 0.0775 0.1071 0.0381 0.0803 0.162 0.1053 0.0705 0.1025 0.0657 0.0747 0.1443 0.1091
Cytoplasmic Foci 0.0597 0.033 0.0234 0.0172 0.0299 0.0293 0.0674 0.0396 0.0294 0.0313 0.017 0.0194 0.0463 0.0263 0.0345 0.0178 0.0158 0.0224
Eisosomes 0.0002 0.0001 0.0004 0.0007 0.0002 0.0002 0.0007 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0005
Endoplasmic Reticulum 0.016 0.0039 0.0048 0.0048 0.002 0.003 0.0053 0.0023 0.0009 0.0021 0.003 0.0012 0.0029 0.0026 0.0024 0.0029 0.004 0.0021
Endosome 0.0498 0.0094 0.0194 0.0204 0.0354 0.0093 0.0357 0.0073 0.0061 0.0098 0.0354 0.007 0.0166 0.0095 0.0097 0.0135 0.0315 0.0088
Golgi 0.0076 0.0007 0.0068 0.0044 0.0075 0.0012 0.0064 0.0006 0.0015 0.0031 0.0071 0.0009 0.0034 0.0006 0.0015 0.003 0.0041 0.0016
Lipid Particles 0.0221 0.005 0.0074 0.0061 0.0157 0.0067 0.0235 0.0029 0.0057 0.0073 0.0168 0.0078 0.012 0.0031 0.0045 0.0206 0.0109 0.0028
Mitochondria 0.0052 0.0012 0.0084 0.033 0.0049 0.0019 0.024 0.0012 0.0127 0.0656 0.005 0.0011 0.0052 0.0013 0.0012 0.004 0.0138 0.0012
None 0.4012 0.4515 0.378 0.279 0.5452 0.3834 0.2814 0.3295 0.1564 0.2508 0.3033 0.3573 0.2284 0.2664 0.1156 0.2464 0.1894 0.2176
Nuclear Periphery 0.0514 0.0135 0.0138 0.0305 0.0113 0.0286 0.0259 0.0159 0.0173 0.0247 0.0316 0.0177 0.0206 0.0198 0.0223 0.0189 0.0377 0.0154
Nucleolus 0.0062 0.0092 0.0136 0.0107 0.0048 0.0072 0.0086 0.0102 0.0148 0.0044 0.0055 0.0141 0.0085 0.0112 0.0091 0.0196 0.0097 0.0117
Nucleus 0.0414 0.1162 0.0988 0.2414 0.1104 0.1271 0.0659 0.1331 0.135 0.0704 0.2307 0.1993 0.1366 0.2175 0.1652 0.1243 0.2506 0.2229
Peroxisomes 0.0152 0.0014 0.0034 0.0027 0.0091 0.0069 0.0253 0.0044 0.0145 0.0116 0.0176 0.0049 0.0156 0.0015 0.0029 0.0127 0.0129 0.0014
Punctate Nuclear 0.1977 0.1991 0.2997 0.2043 0.0919 0.2372 0.2804 0.3392 0.5599 0.43 0.1542 0.2565 0.4124 0.3271 0.547 0.412 0.2592 0.3717
Vacuole 0.0045 0.0027 0.0036 0.0047 0.0031 0.0031 0.0048 0.0016 0.0008 0.0007 0.0035 0.0009 0.0021 0.0027 0.0014 0.0015 0.0038 0.001
Vacuole Periphery 0.0027 0.0005 0.0008 0.0018 0.0008 0.0014 0.0021 0.0003 0.0003 0.0005 0.0018 0.0004 0.0007 0.0009 0.0006 0.0007 0.0017 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 14.647 7.7591 1.4394 7.408 12.6603 13.0123 10.7872 8.9353 6.9099 15.6895
Translational Efficiency – – – – – – – – – –

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✔
Translational Efficiency –
Standard name
Human Ortholog
Description Mitotic spindle protein; interacts with components of the Dam1 (DASH) complex, its effector Sli15p, and microtubule-associated protein Bim1p; also localizes to nuclear microtubules and to the bud neck in a ring-shaped structure; inhibits dynein function
Localization
Cell Percentages nucleus (25%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-3

She1

She1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (She1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available