Standard name | |
---|---|
Human Ortholog | |
Description | Ribosomal 60S subunit protein L19B; rpl19a and rpl19b single null mutations result in slow growth, while the double null mutation is lethal; homologous to mammalian ribosomal protein L19, no bacterial homolog; RPL19B has a paralog, RPL19A, that arose from the whole genome duplication |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 3 | 2 | 3 | 7 | 14 | 8 | 13 | 7 | 11 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud | 1 | 1 | 0 | 1 | 0 | 0 | 3 | 3 | 1 | 3 | 3 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 7 | 16 | 18 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 3 | 0 | 0 | 4 | 9 | 10 | 16 | 19 | 7 | 16 | 4 | 18 | 6 | 19 | 22 | 3 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 137 | 155 | 174 | 185 | 237 | 227 | 278 | 339 | 144 | 193 | 148 | 145 | 397 | 546 | 477 | 267 | 234 | 231 | 138 | 147 | 166 | 290 | 406 | 402 |
Endoplasmic Reticulum | 6 | 5 | 5 | 11 | 8 | 13 | 15 | 61 | 17 | 22 | 12 | 18 | 18 | 14 | 2 | 13 | 16 | 9 | 2 | 1 | 3 | 9 | 5 | 10 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Golgi | 1 | 0 | 0 | 5 | 0 | 6 | 8 | 14 | 5 | 7 | 6 | 6 | 0 | 6 | 6 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 1 | 0 | 1 | 1 | 0 | 10 | 10 | 8 | 9 | 11 | 4 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 1 |
Vac/Vac Membrane | 0 | 0 | 2 | 3 | 10 | 4 | 3 | 16 | 11 | 11 | 11 | 9 | 3 | 3 | 1 | 1 | 1 | 1 | 0 | 5 | 4 | 5 | 5 | 3 |
Unique Cell Count | 140 | 160 | 180 | 201 | 258 | 252 | 319 | 428 | 179 | 248 | 191 | 187 | 409 | 568 | 485 | 273 | 234 | 232 | 145 | 163 | 180 | 323 | 449 | 444 |
Labelled Cell Count | 148 | 162 | 181 | 213 | 268 | 263 | 341 | 477 | 201 | 276 | 204 | 213 | 428 | 588 | 509 | 286 | 252 | 242 | 145 | 163 | 180 | 323 | 449 | 444 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 63.7 | 80.1 | 59.9 | 66.2 | 49.3 | 35.8 | 31.3 | 28.5 | 25.0 | 25.6 | 24.6 | 27.0 | 60.5 | 58.1 | 57.0 | 175.4 | 180.3 | 172.1 | 63.9 | 64.0 | 64.1 |
Std Deviation (1e-4) | 35.2 | 50.8 | 35.0 | 38.2 | 27.7 | 20.7 | 17.3 | 15.5 | 14.2 | 13.7 | 14.0 | 14.7 | 38.8 | 36.6 | 35.5 | 55.6 | 60.8 | 61.4 | 40.7 | 40.5 | 40.4 |
Intensity Change (Log2) | – | – | – | 0.14 | -0.28 | -0.74 | -0.94 | -1.07 | -1.26 | -1.23 | -1.28 | -1.15 | 0.01 | -0.04 | -0.07 | 1.55 | 1.59 | 1.52 | 0.09 | 0.1 | 0.1 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 3.1 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 3.1 | 2.9 | 0 | 3.5 | 0 | 4.3 | 0 | 0 | 2.9 | 0 | 0 | 0 |
Cytoplasm | -1.9 | -2.1 | -2.6 | -3.5 | -5.4 | -4.8 | -5.5 | -5.5 | -5.4 | 0.3 | -0.3 | 1.3 | 0.7 | 2.8 | 2.3 |
Endoplasmic Reticulum | 1.3 | 0.2 | 1.2 | 1.1 | 4.2 | 2.7 | 2.6 | 1.6 | 2.7 | 0.9 | 0 | 0 | 1.1 | 1.9 | 0.6 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 1.7 | 2.6 | 2.0 | 2.4 | 2.1 | 0 | 0 | 0 | 0 | 0 | 0 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 60.7286 | 77.9037 | 74.6911 | 61.8647 | 59.9554 | 77.082 | 53.7733 | 63.1834 | 58.596 | 57.3699 | 42.8054 | 57.3033 | 70.6352 | 88.2791 | 73.6984 | 73.6897 | 70.5594 | 88.3153 |
Actin | 0.0008 | 0.003 | 0.0006 | 0.0001 | 0.0008 | 0.0007 | 0.0022 | 0.0001 | 0.0022 | 0.0001 | 0.0027 | 0.0093 | 0.0005 | 0.0014 | 0.0006 | 0.0004 | 0.0034 | 0.0021 |
Bud | 0.0022 | 0.0042 | 0.0039 | 0.0011 | 0.0013 | 0.0012 | 0.0022 | 0.0004 | 0.0012 | 0.0002 | 0.0006 | 0.0011 | 0.0021 | 0.0032 | 0.0037 | 0.005 | 0.0059 | 0.0022 |
Bud Neck | 0.0004 | 0.0006 | 0.001 | 0.0006 | 0.0006 | 0.0012 | 0.0005 | 0.0002 | 0.0036 | 0.0002 | 0.0004 | 0.0016 | 0.0004 | 0.0003 | 0.001 | 0.0005 | 0.0008 | 0.0024 |
Bud Periphery | 0.0023 | 0.0032 | 0.0018 | 0.0008 | 0.0007 | 0.0008 | 0.0017 | 0.0007 | 0.0025 | 0.0001 | 0.0009 | 0.0005 | 0.0015 | 0.0011 | 0.0015 | 0.0052 | 0.0022 | 0.002 |
Bud Site | 0.001 | 0.0054 | 0.002 | 0.0003 | 0.0004 | 0.0002 | 0.0023 | 0.0002 | 0.0009 | 0.0001 | 0.0005 | 0.0001 | 0.0004 | 0.0041 | 0.0008 | 0.0005 | 0.0013 | 0.0002 |
Cell Periphery | 0.0219 | 0.0083 | 0.0128 | 0.0144 | 0.0111 | 0.0085 | 0.0052 | 0.001 | 0.0031 | 0.0007 | 0.0006 | 0.0013 | 0.0128 | 0.0043 | 0.0095 | 0.0017 | 0.0025 | 0.0065 |
Cytoplasm | 0.7802 | 0.9232 | 0.8699 | 0.8117 | 0.8186 | 0.8485 | 0.8384 | 0.9813 | 0.9283 | 0.9056 | 0.812 | 0.9193 | 0.8303 | 0.9468 | 0.8922 | 0.8327 | 0.8868 | 0.842 |
Cytoplasmic Foci | 0.0064 | 0.0017 | 0.0031 | 0.0027 | 0.0117 | 0.0069 | 0.0124 | 0.001 | 0.0041 | 0.0038 | 0.0388 | 0.006 | 0.0039 | 0.0045 | 0.004 | 0.0052 | 0.0057 | 0.0048 |
Eisosomes | 0.0003 | 0.0001 | 0 | 0 | 0 | 0.0002 | 0.0003 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0 | 0.0002 | 0 | 0 | 0 |
Endoplasmic Reticulum | 0.0224 | 0.0079 | 0.0041 | 0.0111 | 0.0048 | 0.0481 | 0.0191 | 0.0029 | 0.0093 | 0.0215 | 0.0076 | 0.0067 | 0.033 | 0.0031 | 0.0114 | 0.0027 | 0.0058 | 0.0347 |
Endosome | 0.0095 | 0.0022 | 0.007 | 0.0037 | 0.0415 | 0.0159 | 0.019 | 0.0011 | 0.0061 | 0.034 | 0.0291 | 0.0149 | 0.0132 | 0.004 | 0.0099 | 0.0086 | 0.0155 | 0.0165 |
Golgi | 0.0012 | 0.0003 | 0.0007 | 0.0002 | 0.0052 | 0.0031 | 0.0045 | 0.0002 | 0.0007 | 0.0015 | 0.0062 | 0.0031 | 0.0014 | 0.0013 | 0.0007 | 0.0002 | 0.001 | 0.002 |
Lipid Particles | 0.0023 | 0.0005 | 0.0013 | 0.0023 | 0.0022 | 0.0011 | 0.0072 | 0.0002 | 0.0006 | 0.0003 | 0.0225 | 0.0006 | 0.0013 | 0.0007 | 0.001 | 0.0002 | 0.0007 | 0.0014 |
Mitochondria | 0.0067 | 0.0005 | 0.0018 | 0.0006 | 0.0018 | 0.0015 | 0.0174 | 0.0002 | 0.0019 | 0.0004 | 0.0298 | 0.0025 | 0.0016 | 0.0008 | 0.0011 | 0.0019 | 0.0006 | 0.0012 |
None | 0.0059 | 0.0007 | 0.0011 | 0.0007 | 0.0013 | 0.0009 | 0.0048 | 0.0002 | 0.0012 | 0.0003 | 0.0092 | 0.0009 | 0.0008 | 0.0005 | 0.0011 | 0.0014 | 0.0005 | 0.0007 |
Nuclear Periphery | 0.0076 | 0.0017 | 0.0035 | 0.0047 | 0.0061 | 0.0061 | 0.0039 | 0.0007 | 0.0024 | 0.0024 | 0.005 | 0.0015 | 0.0165 | 0.0012 | 0.0052 | 0.0048 | 0.0031 | 0.02 |
Nucleolus | 0.0099 | 0.0002 | 0.0006 | 0.003 | 0.0045 | 0.0004 | 0.002 | 0.0001 | 0.0005 | 0.0001 | 0.0048 | 0.0003 | 0.0005 | 0.0003 | 0.0008 | 0.0084 | 0.0002 | 0.0005 |
Nucleus | 0.0063 | 0.0019 | 0.0033 | 0.0052 | 0.0059 | 0.0026 | 0.004 | 0.0006 | 0.0032 | 0.0012 | 0.0043 | 0.0014 | 0.0036 | 0.0014 | 0.0053 | 0.0058 | 0.0021 | 0.0045 |
Peroxisomes | 0.0003 | 0.0001 | 0.0006 | 0.0001 | 0.0001 | 0.0001 | 0.0019 | 0 | 0.0002 | 0 | 0.0015 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Punctate Nuclear | 0.0004 | 0.0001 | 0.0002 | 0.0001 | 0.0002 | 0.0003 | 0.0018 | 0 | 0.0001 | 0 | 0.0032 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0002 |
Vacuole | 0.096 | 0.0317 | 0.0736 | 0.118 | 0.0607 | 0.04 | 0.0409 | 0.0073 | 0.0239 | 0.0232 | 0.0133 | 0.0222 | 0.0617 | 0.0183 | 0.0427 | 0.0862 | 0.0495 | 0.0411 |
Vacuole Periphery | 0.0162 | 0.0026 | 0.0071 | 0.0186 | 0.0207 | 0.0118 | 0.0084 | 0.0017 | 0.0041 | 0.0042 | 0.0065 | 0.0064 | 0.0143 | 0.0026 | 0.0071 | 0.0284 | 0.0119 | 0.015 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 2644.2991 | 3081.9929 | 3550.5204 | 2529.0617 | 3326.8006 | 3242.6001 | 2498.5589 | 2319.3339 | 2808.7746 | 2192.9477 |
Translational Efficiency | 1.244 | 1.1436 | 1.0321 | 1.5378 | 1.3204 | 1.0668 | 1.1736 | 1.438 | 1.1096 | 1.4799 |
Dataset | Hit |
---|---|
Protein Concentration | ✔ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
R1 | R2 | R1 & R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 |
1612 | 1123 | 219 | 439 | 2327 | 2146 | 339 | 1174 | 3939 | 3269 | 558 | 1613 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Mean | 3657.30 | 4441.02 | 5442.13 | 4567.45 | 4734.23 | 5090.98 | 5263.00 | 6176.76 | 4293.51 | 4867.70 | 5333.30 | 5738.76 |
Standard Deviation | 2052.21 | 2559.25 | 3156.77 | 2416.35 | 2582.90 | 2769.86 | 3007.73 | 3164.54 | 2438.26 | 2716.95 | 3068.33 | 3064.46 |
Intensity Change Log 2 | — | 0.280112 | 0.573392 | 0.320610 | — | 0.104814 | 0.152756 | 0.383720 | — | 0.183845 | 0.351297 | 0.356554 |
R1 | R2 | R1 & R2 | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|
WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | WT | UBP2 | UBP14 | UBP2UBP14 | |
Actin | 0.000142 | 0.000154 | 0.000256 | 0.000195 | 0.000095 | 0.000160 | 0.000289 | 0.000177 | 0.000114 | 0.000158 | 0.000276 | 0.000182 |
Bud Neck | 0.001174 | 0.000940 | 0.009158 | 0.008028 | 0.000822 | 0.001376 | 0.010705 | 0.004611 | 0.000966 | 0.001226 | 0.010098 | 0.005541 |
Bud Site | 0.010716 | 0.008797 | 0.012651 | 0.010014 | 0.011688 | 0.015116 | 0.009443 | 0.007707 | 0.011290 | 0.012945 | 0.010702 | 0.008335 |
Cell Periphery | 0.014283 | 0.017312 | 0.034972 | 0.014700 | 0.010234 | 0.012383 | 0.013311 | 0.010838 | 0.011891 | 0.014077 | 0.021812 | 0.011889 |
Cytoplasm | 0.718972* | 0.676568* | 0.371099* | 0.345021* | 0.738107* | 0.767477* | 0.366947* | 0.532500* | 0.730276* | 0.736247* | 0.368577* | 0.481475* |
Cytoplasmic Foci | 0.029541 | 0.021652 | 0.050679 | 0.028015 | 0.024661 | 0.028629 | 0.051468 | 0.042124 | 0.026658 | 0.026232 | 0.051159 | 0.038284 |
Eisosomes | 0.000003 | 0.000003 | 0.000009 | 0.000006 | 0.000002 | 0.000003 | 0.000008 | 0.000004 | 0.000002 | 0.000003 | 0.000008 | 0.000005 |
Endoplasmic Reticulum | 0.002046 | 0.002086 | 0.000619 | 0.000984 | 0.002240 | 0.001678 | 0.001155 | 0.000434 | 0.002161 | 0.001818 | 0.000945 | 0.000583 |
Endosome | 0.001873 | 0.001600 | 0.002796 | 0.003671 | 0.001703 | 0.001474 | 0.004254 | 0.001947 | 0.001773 | 0.001517 | 0.003681 | 0.002416 |
Golgi | 0.063958 | 0.056521 | 0.080065 | 0.080448 | 0.039927 | 0.032155 | 0.079811 | 0.059879 | 0.049761 | 0.040526 | 0.079911 | 0.065477 |
Lipid Particles | 0.009657 | 0.013353 | 0.035298 | 0.016250 | 0.006789 | 0.007640 | 0.023401 | 0.010448 | 0.007963 | 0.009603 | 0.028070 | 0.012027 |
Mitochondria | 0.000331 | 0.000426 | 0.000334 | 0.000323 | 0.000547 | 0.000684 | 0.000288 | 0.000572 | 0.000459 | 0.000595 | 0.000306 | 0.000504 |
Mitotic Spindle | 0.001562 | 0.000722 | 0.008328 | 0.006038 | 0.001338 | 0.001365 | 0.006523 | 0.006454 | 0.001430 | 0.001144 | 0.007231 | 0.006341 |
None | 0.002886 | 0.005729 | 0.001766 | 0.001440 | 0.003577 | 0.005543 | 0.002040 | 0.003249 | 0.003294 | 0.005607 | 0.001932 | 0.002757 |
Nuclear Periphery | 0.000592 | 0.000574 | 0.003496 | 0.003129 | 0.000398 | 0.000451 | 0.004104 | 0.003171 | 0.000478 | 0.000493 | 0.003865 | 0.003160 |
Nuclear Periphery Foci | 0.012644 | 0.014314 | 0.035572 | 0.036716 | 0.007988 | 0.009735 | 0.041374 | 0.026491 | 0.009893 | 0.011308 | 0.039097 | 0.029274 |
Nucleolus | 0.000122 | 0.000205 | 0.000221 | 0.000345 | 0.000118 | 0.000141 | 0.000298 | 0.000281 | 0.000120 | 0.000163 | 0.000267 | 0.000298 |
Nucleus | 0.014848 | 0.016738 | 0.028385 | 0.018686 | 0.013893 | 0.019589 | 0.030058 | 0.036751 | 0.014283 | 0.018609 | 0.029402 | 0.031834 |
Peroxisomes | 0.000029 | 0.000065 | 0.000065 | 0.000027 | 0.000048 | 0.000051 | 0.000033 | 0.000025 | 0.000040 | 0.000056 | 0.000046 | 0.000025 |
Vacuole | 0.058213 | 0.095222 | 0.224566* | 0.296153* | 0.058070 | 0.052006 | 0.272323* | 0.161607 | 0.058129 | 0.066852 | 0.253580* | 0.198226 |
Vacuole Periphery | 0.056409 | 0.067018 | 0.099666 | 0.129810* | 0.077757 | 0.042345 | 0.082167 | 0.090729 | 0.069020 | 0.050821 | 0.089035 | 0.101365* |
R1 | R2 | R1 & R2 | |||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | UBP2_WT | UBP14_WT | UBP2UBP14_WT | UBP2UBP14_UBP2 | UBP2UBP14_UBP14 | |
Actin | -0.72 | -4.36 | -3.55 | -2.31 | 1.79 | -3.82 | -6.76 | -6.82 | -1.25 | 3.71 | -3.50 | -8.02 | -7.32 | -2.26 | 4.19 |
Bud Neck | 1.57 | -6.47 | -8.31 | -8.51 | 1.08 | -5.52 | -11.25 | -13.30 | -11.15 | 6.42 | -3.67 | -12.56 | -14.94 | -13.92 | 5.77 |
Bud Site | 1.53 | -1.52 | -0.10 | -1.05 | 1.17 | -3.23 | 0.68 | 3.68 | 6.10 | 1.67 | -2.13 | -0.66 | 2.54 | 4.16 | 2.29 |
Cell Periphery | -1.81 | -5.44 | 0.36 | 1.82 | 5.36 | -2.30 | -2.59 | -0.61 | 1.56 | 2.02 | -2.55 | -5.77 | 0.40 | 2.66 | 5.80 |
Cytoplasm | 3.38 | 15.24 | 21.10 | 17.55 | 0.55 | -2.98 | 20.52 | 19.01 | 21.20 | -8.03 | -0.48 | 25.53 | 26.28 | 25.93 | -6.99 |
Cytoplasmic Foci | 3.09 | -3.76 | -1.08 | -3.21 | 3.08 | -2.65 | -6.69 | -8.44 | -6.52 | 2.04 | -0.14 | -7.50 | -7.79 | -7.49 | 3.11 |
Eisosomes | -0.42 | -5.84 | -4.88 | -4.23 | 2.76 | -3.07 | -8.64 | -7.78 | -3.83 | 5.33 | -2.64 | -10.36 | -8.87 | -5.58 | 6.08 |
Endoplasmic Reticulum | -0.08 | 4.00 | 2.87 | 2.48 | -1.69 | 1.60 | 3.20 | 6.96 | 5.08 | 1.99 | 1.12 | 4.78 | 7.35 | 5.56 | 1.55 |
Endosome | 1.57 | -3.65 | -5.62 | -6.28 | -1.79 | 1.63 | -7.52 | -2.59 | -4.01 | 6.27 | 2.35 | -8.27 | -5.54 | -7.12 | 4.60 |
Golgi | 1.81 | -1.96 | -2.49 | -3.91 | -0.03 | 3.49 | -6.58 | -5.83 | -8.95 | 3.13 | 4.43 | -6.26 | -4.79 | -8.68 | 2.99 |
Lipid Particles | -2.81 | -5.63 | -4.21 | -1.68 | 4.00 | -1.29 | -8.25 | -4.72 | -3.77 | 6.08 | -2.58 | -9.25 | -5.78 | -3.38 | 7.04 |
Mitochondria | -1.52 | -0.53 | -0.89 | 0.72 | -0.18 | -2.03 | 2.66 | -1.24 | 0.44 | -2.87 | -2.76 | 1.64 | -1.86 | 0.43 | -2.76 |
Mitotic Spindle | 2.57 | -4.40 | -5.07 | -6.02 | 1.05 | -0.22 | -5.40 | -6.30 | -6.25 | -0.16 | 1.56 | -6.85 | -7.77 | -8.24 | 0.64 |
None | -2.87 | 3.79 | 5.51 | 4.35 | 1.18 | -2.69 | 4.59 | 1.06 | 3.09 | -3.55 | -3.93 | 5.83 | 2.37 | 4.77 | -3.40 |
Nuclear Periphery | 0.13 | -7.28 | -7.51 | -7.80 | 0.81 | -1.50 | -7.54 | -12.20 | -11.92 | 1.56 | -0.45 | -10.10 | -14.04 | -14.15 | 1.73 |
Nuclear Periphery Foci | -1.20 | -6.16 | -8.16 | -7.29 | -0.19 | -2.39 | -9.53 | -10.51 | -9.17 | 3.59 | -2.08 | -11.29 | -12.68 | -11.35 | 3.14 |
Nucleolus | -1.64 | -4.22 | -5.38 | -2.20 | -2.77 | -1.62 | -7.69 | -13.06 | -7.88 | 0.94 | -2.16 | -8.68 | -11.91 | -5.85 | -1.08 |
Nucleus | -1.26 | -4.32 | -3.28 | -2.49 | 1.84 | -5.31 | -7.09 | -12.77 | -9.61 | -1.50 | -5.11 | -8.24 | -12.89 | -9.92 | -0.83 |
Peroxisomes | -0.96 | -1.56 | -1.00 | 0.89 | 1.35 | -0.35 | 1.19 | 3.21 | 3.70 | 2.95 | -1.07 | -1.03 | 2.45 | 1.49 | 2.07 |
Vacuole | -6.71 | -11.46 | -19.28 | -15.54 | -3.71 | 1.67 | -15.52 | -16.99 | -17.75 | 7.25 | -3.19 | -19.17 | -24.33 | -22.18 | 4.74 |
Vacuole Periphery | -2.21 | -3.86 | -8.40 | -6.67 | -2.47 | 9.92 | 0.70 | -1.16 | -9.57 | -1.43 | 6.45 | -2.08 | -6.10 | -11.04 | -2.09 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
---|
Gene | Images |
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Gene | Images |
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