Standard name
Human Ortholog
Description S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0.09 0.08 0.05 0.07 0.05 0 – 0 0 0 0 0.1 0.12 0.13 0.12 0.11 0.11 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 – 0 0 0.06 0 0 0 0 0 0 0.07 0 0 0 0 0 0
Nucleus 0.95 0.89 0.87 0.92 0.86 0.89 0.84 – 0.83 0.84 0.77 0.82 0.88 0.84 0.81 0.85 0.85 0.83 0.96 0.92 0.89 0.83 0.69 0.56
Nuclear Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.06 0 0 0.05 0.07 0.11 0.18 – 0.21 0.23 0.31 0.24 0 0.08 0.05 0 0 0 0 0 0 0 0.06 0.1
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0.05 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0.09 0.19 0.27
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 1 0 0 – 0 0 0 0 1 0 0 0 0 0 0 1 1 0 0 0
Bud 0 0 1 0 1 0 0 – 0 1 1 1 0 0 0 0 0 0 1 1 1 2 2 4
Bud Neck 0 0 0 0 0 0 1 – 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 – 3 3 11 11 1 1 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0
Cytoplasm 6 38 20 7 13 17 15 – 13 17 17 8 31 37 47 70 50 37 1 2 5 3 4 4
Endoplasmic Reticulum 0 0 0 0 0 0 0 – 1 1 0 0 0 0 0 5 3 3 0 0 1 0 1 0
Endosome 0 1 0 0 0 1 0 – 1 0 0 0 0 0 0 1 2 1 0 1 1 0 1 1
Golgi 0 0 1 0 0 0 0 – 0 0 0 0 0 0 1 2 2 0 0 0 0 0 0 0
Mitochondria 2 1 0 0 1 3 3 – 10 2 28 14 1 0 1 11 9 22 0 0 1 0 1 5
Nucleus 197 362 218 121 164 302 408 – 402 427 365 339 274 259 286 492 373 280 204 389 239 158 225 198
Nuclear Periphery 0 1 0 0 0 0 1 – 3 0 3 5 0 1 1 0 0 0 0 0 0 0 0 1
Nucleolus 12 16 4 6 14 39 88 – 102 116 148 98 13 24 19 24 15 10 1 6 4 6 20 36
Peroxisomes 0 0 0 0 0 0 0 – 0 0 0 0 0 1 0 0 0 0 0 0 0 0 1 0
SpindlePole 0 0 0 0 0 0 0 – 1 0 0 0 4 6 10 0 1 0 0 0 0 0 0 0
Vac/Vac Membrane 0 6 12 2 6 3 4 – 12 7 8 0 2 2 7 19 13 20 2 14 7 17 63 97
Unique Cell Count 207 406 252 132 190 341 488 486 509 477 413 312 307 354 578 441 338 213 423 268 191 328 356
Labelled Cell Count 217 425 256 136 200 365 521 548 574 583 476 327 331 372 625 469 373 213 423 268 191 328 356


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 25.6 43.2 27.8 24.1 22.9 18.2 17.0 – 14.5 15.0 12.8 13.2 29.5 29.2 28.4 51.2 49.0 47.8 26.4 27.8 27.7
Std Deviation (1e-4) 8.1 21.4 10.0 10.2 7.0 5.7 5.7 – 4.5 5.5 5.1 4.7 13.5 15.1 15.8 25.5 22.8 20.9 10.7 12.0 11.8
Intensity Change (Log2) – – – -0.21 -0.28 -0.61 -0.71 – -0.94 -0.89 -1.12 -1.08 0.08 0.07 0.03 0.88 0.81 0.78 -0.08 -0.0 -0.01


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Cytoplasm -1.0 -0.4 -1.5 -3.0 – -3.3 -2.8 -2.6 -3.7 0.8 1.6 2.1 1.8 1.4 1.2
Endoplasmic Reticulum 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 – 0 0 3.9 3.0 0 0 0 0 0 4.1
Nucleus 1.5 -0.1 0.8 -1.0 – -1.3 -0.9 -3.2 -1.5 0.5 -0.7 -1.9 -0.5 -0.7 -1.2
Nuclear Periphery 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 3.0 4.6 6.4 – 7.1 7.6 9.3 7.7 1.8 3.4 2.4 1.9 1.4 1.1
Peroxisomes 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0
Vacuole 0 -0.8 -3.0 -3.5 – -1.7 -2.8 -2.4 0 -3.1 -3.1 -1.9 -1.0 -1.2 0.6

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 26.0992 25.202 22.2025 22.7527 16.7921 25.1862 31.1322 29.5603 27.4621 25.9298 25.6973 27.7906 37.8546 33.1109 33.3034 31.5739 29.9708 32.3761
Actin 0.0004 0 0 0 0.0097 0 0.0031 0 0.0015 0.0006 0.0313 0.0003 0 0 0 0 0 0
Bud 0 0 0.0002 0.0001 0.0018 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0.0001 0 0 0 0 0 0
Bud Neck 0.0001 0 0.0001 0 0.0013 0.0002 0.0001 0 0.0007 0 0.0002 0.0002 0 0 0 0 0 0.0002
Bud Periphery 0 0 0.0001 0.0001 0.0023 0.0001 0.0001 0 0.0002 0 0.0002 0.0001 0 0 0 0 0 0
Bud Site 0.0001 0.0001 0.0001 0.0001 0.0318 0 0.0002 0.0001 0.0003 0.0001 0.0002 0 0.0001 0 0 0 0 0
Cell Periphery 0.0001 0.0001 0.0003 0.0002 0.0023 0.0001 0.0002 0.0001 0.0009 0.0001 0.0006 0.0002 0.0001 0.0001 0 0 0.0001 0.0001
Cytoplasm 0.0012 0 0.0003 0 0.0016 0.002 0.0018 0.0004 0.0005 0.0003 0.0004 0.0002 0.0003 0 0 0 0 0
Cytoplasmic Foci 0.013 0.0095 0.0039 0.0007 0.0173 0.0028 0.0053 0.0025 0.0074 0.015 0.0127 0.006 0.0033 0 0 0 0 0
Eisosomes 0 0 0 0 0.0001 0 0 0 0 0.0001 0.0001 0 0.0001 0 0 0 0 0
Endoplasmic Reticulum 0.0011 0 0 0 0.0007 0.0002 0.0006 0 0.0002 0 0.0002 0 0 0 0 0 0 0
Endosome 0.003 0.0001 0.0031 0 0.0289 0.015 0.0085 0 0.0059 0.0001 0.0095 0.0009 0 0 0 0 0 0
Golgi 0.0002 0.0001 0.0002 0 0.0511 0.0006 0.0022 0 0.006 0.0001 0.0026 0.0014 0 0 0 0 0 0
Lipid Particles 0.0029 0.0015 0.0028 0.0031 0.0345 0.0001 0.0044 0.0003 0.0022 0.0016 0.0131 0.0015 0.0006 0 0 0 0 0
Mitochondria 0.0028 0.0011 0.0014 0.0106 0.0151 0.0008 0.0026 0.0002 0.0047 0.0075 0.0027 0.0007 0.0009 0.0001 0.0001 0 0.0006 0.0001
None 0.001 0 0.0003 0.0001 0.0005 0 0.0015 0.0002 0.0002 0.0014 0.0004 0.0002 0.0011 0 0 0 0 0
Nuclear Periphery 0.0082 0.0012 0.0004 0.0003 0.001 0.0002 0.0071 0.0044 0.0024 0.0018 0.0048 0.0005 0.0002 0.002 0.0001 0.0001 0.0002 0.0001
Nucleolus 0.0292 0.023 0.0212 0.0267 0.196 0.0633 0.0477 0.0098 0.0308 0.057 0.2084 0.0919 0.0313 0.0183 0.0142 0.0115 0.1533 0.0914
Nucleus 0.9332 0.9601 0.964 0.9547 0.5713 0.9135 0.9082 0.9807 0.9287 0.9069 0.6975 0.892 0.9608 0.9791 0.9855 0.9884 0.8456 0.9077
Peroxisomes 0.0007 0.002 0.0002 0.0003 0.0055 0 0.0008 0 0.0008 0.0003 0.0019 0.0012 0.0001 0 0 0 0 0
Punctate Nuclear 0.0015 0.0004 0.0001 0 0.0034 0 0.0035 0.0002 0.0004 0.0061 0.0006 0.0016 0.0007 0.0001 0 0 0 0
Vacuole 0.0005 0.0002 0.0009 0.0008 0.0154 0.0005 0.0008 0.0006 0.0023 0.0002 0.0025 0.0005 0.0002 0.0001 0 0 0.0001 0.0002
Vacuole Periphery 0.0006 0.0005 0.0004 0.0022 0.0082 0.0004 0.001 0.0003 0.0037 0.0009 0.01 0.0005 0.0002 0.0001 0 0 0.0001 0.0001

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 93.4269 91.6778 112.9066 79.2567 81.1028 69.7631 78.0145 72.226 65.1439 76.5895
Translational Efficiency 1.4536 1.7251 1.113 1.5069 1.4283 1.6085 1.4973 1.3713 1.3645 1.4407

Hit Data

Dataset Hit
Protein Concentration ✔
Protein Localization ✘
Gene Expression ✔
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1829 1535 925 1602 1557 2078 123 83 3386 3613 1048 1685

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 1308.54 2167.84 2697.19 3168.99 1879.78 2124.25 2865.20 3184.59 1571.22 2142.77 2716.91 3169.76
Standard Deviation 299.56 574.66 578.79 834.63 443.89 508.28 527.54 840.58 469.18 537.92 575.56 834.94
Intensity Change Log 2 — 0.728300 1.043499 1.276065 — 0.176390 0.608072 0.760544 — 0.428884 0.802908 0.994773

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000482 0.000467 0.000445 0.000577 0.000502 0.000583 0.000564 0.000472 0.000491 0.000534 0.000459 0.000572
Bud Neck 0.001114 0.001376 0.003878 0.006430 0.001578 0.002255 0.003596 0.004224 0.001328 0.001881 0.003845 0.006321
Bud Site 0.000198 0.000336 0.000349 0.001173 0.000175 0.000315 0.000441 0.000815 0.000188 0.000324 0.000359 0.001155
Cell Periphery 0.000741 0.000585 0.000062 0.000235 0.000346 0.000822 0.000020 0.000056 0.000560 0.000721 0.000057 0.000226
Cytoplasm 0.000744 0.000135 0.000036 0.000497 0.000450 0.000196 0.000011 0.000146 0.000609 0.000170 0.000033 0.000480
Cytoplasmic Foci 0.000026 0.000485 0.000139 0.000824 0.000060 0.000139 0.000162 0.000925 0.000042 0.000286 0.000142 0.000829
Eisosomes 0.000852 0.000889 0.000267 0.000121 0.000779 0.001250 0.000307 0.000084 0.000819 0.001097 0.000272 0.000119
Endoplasmic Reticulum 0.001464 0.000400 0.000196 0.000333 0.000655 0.000997 0.000198 0.000172 0.001092 0.000743 0.000196 0.000325
Endosome 0.000023 0.000064 0.000002 0.000669 0.000013 0.000152 0.000002 0.004372 0.000018 0.000115 0.000002 0.000851
Golgi 0.000009 0.000042 0.000012 0.000484 0.000018 0.000153 0.000013 0.002756 0.000013 0.000106 0.000012 0.000596
Lipid Particles 0.000002 0.000056 0.000002 0.000070 0.000003 0.000014 0.000003 0.000012 0.000003 0.000032 0.000002 0.000068
Mitochondria 0.000624 0.000691 0.001015 0.002007 0.000788 0.000641 0.001175 0.001728 0.000700 0.000662 0.001034 0.001993
Mitotic Spindle 0.000000 0.000649 0.000008 0.002404 0.000001 0.000042 0.000009 0.002967 0.000001 0.000299 0.000009 0.002432
None 0.001448 0.001451 0.000306 0.000655 0.000621 0.000660 0.000247 0.000387 0.001068 0.000996 0.000299 0.000642
Nuclear Periphery 0.000018 0.000020 0.000021 0.000230 0.000031 0.000039 0.000012 0.000072 0.000024 0.000031 0.000020 0.000222
Nuclear Periphery Foci 0.000006 0.000039 0.000001 0.000056 0.000037 0.000030 0.000000 0.000066 0.000020 0.000034 0.000001 0.000056
Nucleolus 0.008285 0.005736 0.006085 0.009184 0.010312 0.005744 0.013290 0.003264 0.009217 0.005741 0.006930 0.008892
Nucleus 0.978180* 0.983706* 0.985147* 0.972036* 0.979394* 0.981135* 0.975069* 0.962914* 0.978738* 0.982227* 0.983964* 0.971587*
Peroxisomes 0.000364 0.000931 0.001761 0.000709 0.000872 0.000476 0.004590 0.000559 0.000598 0.000669 0.002093 0.000701
Vacuole 0.004913 0.001472 0.000203 0.000941 0.002443 0.003614 0.000234 0.007887 0.003777 0.002704 0.000207 0.001283
Vacuole Periphery 0.000506 0.000470 0.000065 0.000367 0.000920 0.000745 0.000056 0.006122 0.000697 0.000628 0.000064 0.000650

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 0.53 1.31 -3.11 -3.20 -3.93 -2.51 -1.67 -0.57 -0.05 -0.15 -2.00 1.33 -3.04 -1.53 -3.65
Bud Neck -1.11 -14.96 -7.82 -7.01 -3.91 -2.52 -3.89 -4.06 -3.10 -1.44 -2.98 -14.59 -7.79 -6.72 -4.03
Bud Site -0.97 -4.25 -3.71 -2.76 -3.17 -1.81 -3.15 -1.97 -1.56 -1.26 -1.78 -6.16 -3.88 -3.20 -3.23
Cell Periphery 0.58 4.95 3.40 1.44 -3.60 -2.01 3.77 3.15 3.45 -1.12 -0.90 5.94 3.35 2.91 -3.71
Cytoplasm 1.47 1.69 0.58 -2.56 -3.32 0.98 1.88 1.41 0.79 -2.39 1.70 2.34 0.55 -2.01 -3.38
Cytoplasmic Foci -2.29 -3.90 -3.34 -1.27 -2.90 -1.07 -1.87 -1.43 -1.28 -1.25 -2.57 -3.77 -3.42 -2.29 -3.01
Eisosomes -0.16 3.61 4.66 4.51 3.48 -3.26 4.88 7.41 10.30 7.05 -2.06 5.39 7.35 10.06 4.08
Endoplasmic Reticulum 8.05 10.20 9.04 1.29 -4.97 -3.25 6.50 3.51 6.08 -0.47 3.86 11.88 9.90 7.56 -5.07
Endosome -0.87 1.54 -2.40 -2.21 -2.48 -1.27 3.24 -1.38 -1.33 -1.38 -1.46 2.20 -2.78 -2.37 -2.84
Golgi -1.59 -1.14 -2.12 -1.96 -2.11 -0.95 0.53 -1.18 -1.12 -1.19 -1.14 0.22 -2.41 -1.90 -2.41
Lipid Particles -1.26 0.78 -1.44 -0.24 -1.46 -1.64 0.67 -1.31 -0.27 -1.39 -1.62 1.47 -1.45 -0.73 -1.47
Mitochondria -0.99 -8.46 -4.83 -4.49 -3.42 2.18 -3.67 -2.44 -2.75 -1.50 0.66 -7.88 -4.76 -4.84 -3.49
Mitotic Spindle -1.11 -2.40 -2.55 -1.58 -2.53 -1.65 -1.21 -1.38 -1.35 -1.37 -1.21 -2.52 -2.68 -2.26 -2.67
None -0.01 7.38 4.43 2.08 -3.85 -0.13 4.14 1.73 0.99 -1.56 0.32 8.11 3.33 1.52 -3.99
Nuclear Periphery -0.65 -1.56 -3.91 -3.86 -3.82 -0.98 3.55 -1.25 -0.86 -1.81 -1.34 0.70 -3.84 -3.65 -3.88
Nuclear Periphery Foci -0.95 1.16 -2.89 -0.52 -3.47 0.18 1.53 -0.41 -0.57 -1.44 -0.68 1.77 -1.84 -0.97 -3.63
Nucleolus 2.86 1.82 -1.13 -3.30 -2.52 3.91 -0.53 1.03 -0.22 1.00 4.74 1.78 -0.33 -3.36 -1.70
Nucleus -2.54 -3.63 2.59 4.38 5.16 -0.82 0.59 1.13 1.22 0.79 -2.32 -2.99 3.14 4.36 4.74
Peroxisomes -5.28 -6.78 -4.11 1.51 4.77 3.41 -2.71 -0.03 -1.02 2.61 -1.00 -6.13 -1.51 -0.69 5.39
Vacuole 4.22 6.08 4.83 1.49 -2.69 -1.83 4.51 -0.95 -0.72 -1.39 1.93 7.51 4.21 3.27 -2.89
Vacuole Periphery 0.14 4.26 0.96 0.41 -3.32 0.62 4.08 -1.20 -1.24 -1.42 0.37 5.63 0.27 -0.00 -2.67
Standard name
Human Ortholog
Description S-adenosyl-L-methionine-dependent tRNA: m5C-methyltransferase; methylates cytosine to m5C at several positions in tRNAs and intron-containing pre-tRNAs; increases proportion of tRNALeu(CAA) with m5C at wobble position in response to hydrogen peroxide, causing selective translation of mRNA from genes enriched in TTG codon; loss of NCL1 confers hypersensitivity to oxidative stress; similar to Nop2p and human proliferation associated nucleolar protein p120
Localization
Cell Percentages nucleus (99%)
Cell Cycle Regulation No
Subcompartmental Group nucleus-2

Ncl1

Ncl1


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ncl1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available