Standard name | |
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Human Ortholog | |
Description | ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 4 | 4 | 2 | 0 | 1 | 0 | 11 | 14 | 9 | 5 | 11 | 13 |
Bud | 12 | 12 | 12 | 7 | 12 | 12 | 12 | 17 | – | 18 | 12 | 9 | 4 | 4 | 1 | 19 | 6 | 7 | 3 | 8 | 2 | 4 | 2 | 1 |
Bud Neck | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 5 | 2 |
Bud Site | 1 | 1 | 4 | 0 | 1 | 5 | 4 | 6 | – | 6 | 7 | 4 | 0 | 0 | 0 | 6 | 6 | 1 | – | – | – | – | – | – |
Cell Periphery | 8 | 5 | 4 | 2 | 2 | 0 | 4 | 1 | – | 2 | 5 | 3 | 3 | 3 | 3 | 9 | 14 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 4 | 10 | 6 | 8 | 8 | 3 | 7 | – | 3 | 2 | 1 | 6 | 6 | 11 | 17 | 22 | 8 | 0 | 2 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | – | 4 | 1 | 3 | 0 | 0 | 1 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 48 | 6 | 24 | 3 | 14 | 3 | 7 | 6 | – | 1 | 0 | 2 | 20 | 19 | 15 | 15 | 17 | 11 | 28 | 30 | 20 | 16 | 29 | 23 |
Golgi | 5 | 0 | 1 | 2 | 5 | 1 | 0 | 2 | – | 0 | 0 | 2 | 55 | 55 | 72 | 4 | 6 | 11 | 27 | 24 | 21 | 18 | 24 | 41 |
Mitochondria | 370 | 220 | 240 | 200 | 218 | 351 | 429 | 543 | – | 465 | 342 | 428 | 133 | 133 | 148 | 17 | 12 | 11 | 384 | 159 | 215 | 163 | 162 | 195 |
Nucleus | 2 | 3 | 1 | 1 | 0 | 2 | 0 | 4 | – | 0 | 1 | 1 | 2 | 2 | 1 | 3 | 9 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 16 | 10 | 11 | 6 | 14 | 26 | 37 | 34 | – | 43 | 36 | 46 | 5 | 6 | 5 | 15 | 7 | 8 | 1 | 0 | 2 | 1 | 3 | 3 |
Nucleolus | 55 | 20 | 19 | 11 | 32 | 52 | 73 | 73 | – | 64 | 30 | 57 | 8 | 8 | 6 | 24 | 14 | 17 | 4 | 3 | 2 | 2 | 3 | 2 |
Peroxisomes | 66 | 38 | 119 | 49 | 36 | 24 | 31 | 20 | – | 11 | 6 | 14 | 16 | 13 | 22 | 68 | 35 | 40 | 37 | 26 | 71 | 34 | 44 | 50 |
SpindlePole | 18 | 8 | 28 | 10 | 8 | 8 | 8 | 17 | – | 4 | 7 | 10 | 13 | 13 | 4 | 22 | 15 | 9 | 17 | 11 | 12 | 13 | 12 | 12 |
Vac/Vac Membrane | 57 | 23 | 10 | 6 | 8 | 12 | 12 | 11 | – | 18 | 5 | 6 | 42 | 43 | 47 | 26 | 12 | 19 | 12 | 11 | 3 | 5 | 3 | 5 |
Unique Cell Count | 491 | 271 | 345 | 245 | 279 | 409 | 492 | 618 | 505 | 359 | 466 | 214 | 215 | 247 | 198 | 141 | 132 | 542 | 301 | 371 | 274 | 307 | 361 | |
Labelled Cell Count | 661 | 354 | 484 | 303 | 359 | 505 | 621 | 742 | 641 | 455 | 588 | 312 | 310 | 338 | 249 | 178 | 156 | 542 | 301 | 371 | 274 | 307 | 361 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 10.0 | 9.5 | 8.5 | 9.2 | 10.1 | 9.6 | 9.3 | 8.6 | – | 8.1 | 7.7 | 8.9 | 10.5 | 10.5 | 10.7 | 8.0 | 8.6 | 11.2 | 10.2 | 11.2 | 11.6 |
Std Deviation (1e-4) | 1.9 | 2.0 | 2.1 | 3.2 | 2.7 | 2.9 | 3.3 | 2.6 | – | 2.6 | 2.4 | 3.0 | 1.9 | 1.9 | 2.3 | 1.9 | 3.0 | 3.2 | 2.6 | 2.6 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.24 | 0.17 | 0.13 | 0.02 | – | -0.06 | -0.14 | 0.07 | 0.31 | 0.31 | 0.34 | -0.08 | 0.02 | 0.4 | 0.27 | 0.4 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.4 | 0.5 | -0.4 | -0.9 | -0.6 | – | 0.1 | -0.1 | -1.4 | -1.1 | -1.1 | -2.5 | 3.0 | 0.4 | 0.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 0 |
Cytoplasm | -0.3 | 0 | -0.8 | -2.6 | -2.0 | – | -2.7 | -2.4 | 0 | -0.1 | -0.1 | 1.0 | 2.9 | 5.1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.3 | -1.0 | -4.6 | -4.2 | -5.1 | – | -5.7 | -5.1 | -5.2 | 1.0 | 0.8 | -0.4 | 0.3 | 1.8 | 0.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 9.7 | 9.7 | 10.5 | 0 | 0 | 0 |
Mitochondria | 3.3 | 2.4 | 5.4 | 6.3 | 7.0 | – | 8.6 | 9.0 | 8.2 | -1.8 | -1.9 | -2.4 | -13.7 | -12.2 | -12.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.5 | 1.2 | 2.0 | 2.7 | 1.6 | – | 3.1 | 3.6 | 3.7 | -0.6 | -0.3 | -0.9 | 2.3 | 0.9 | 1.4 |
Nucleolus | -0.6 | 2.7 | 3.4 | 4.2 | 3.2 | – | 3.5 | 1.5 | 3.2 | -0.9 | -1.0 | -1.8 | 2.7 | 1.8 | 2.7 |
Peroxisomes | -3.8 | -6.2 | -10.0 | -10.5 | -13.2 | – | -12.9 | -11.4 | -12.0 | -7.3 | -7.7 | -7.2 | 0 | -2.1 | -0.9 |
SpindlePole | -2.0 | -2.8 | -4.0 | -4.6 | -3.8 | – | -5.5 | -3.8 | -4.0 | -0.9 | -0.9 | -3.4 | 1.2 | 0.9 | -0.5 |
Vacuole | -0.3 | 0 | 0 | -0.4 | -1.1 | – | 0.5 | -1.4 | -1.6 | 6.6 | 6.7 | 6.6 | 4.6 | 2.7 | 4.7 |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 9.959 | 10.6705 | 10.2788 | 10.1007 | 8.9543 | 10.412 | 9.5216 | 13.3129 | 11.9999 | 11.8646 | 12.8823 | 12.9345 | 3.8127 | 12.8043 | 12.3139 | 10.6606 | 12.0899 | 12.5316 |
Actin | 0.0188 | 0.0048 | 0.0227 | 0.0101 | 0.0007 | 0.0011 | 0.0024 | 0.0013 | 0.0159 | 0.0185 | 0.0291 | 0.009 | 0.0017 | 0.01 | 0.0045 | 0.0189 | 0.0007 | 0.0006 |
Bud | 0.0002 | 0.001 | 0.0001 | 0.0016 | 0 | 0.0001 | 0.0003 | 0 | 0.0001 | 0.0002 | 0.0004 | 0 | 0.0005 | 0.0007 | 0.0002 | 0.0008 | 0.0062 | 0 |
Bud Neck | 0.0004 | 0.0001 | 0.0001 | 0.001 | 0 | 0.0001 | 0.0008 | 0.0001 | 0.0001 | 0.0008 | 0.0002 | 0 | 0.0008 | 0.0011 | 0.0013 | 0.0004 | 0 | 0 |
Bud Periphery | 0.0003 | 0.001 | 0.0003 | 0.0012 | 0.0002 | 0.0002 | 0.0002 | 0.0001 | 0.0003 | 0.0006 | 0.0004 | 0.0001 | 0.0011 | 0.0007 | 0.0002 | 0.001 | 0.0337 | 0.0003 |
Bud Site | 0.0005 | 0.0052 | 0.0002 | 0.0083 | 0 | 0.0001 | 0.0126 | 0.0001 | 0.0003 | 0.0007 | 0.0052 | 0.0001 | 0.0019 | 0.026 | 0.0035 | 0.001 | 0.0006 | 0 |
Cell Periphery | 0.0002 | 0.0006 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0002 | 0.0005 | 0.0001 | 0.0002 | 0.0001 | 0 | 0.0012 | 0.0011 | 0.0002 | 0.0001 | 0.0006 | 0.0001 |
Cytoplasm | 0.0023 | 0.0007 | 0 | 0.0038 | 0.0004 | 0 | 0.0043 | 0 | 0 | 0.0001 | 0.0001 | 0 | 0.0273 | 0.0003 | 0.0001 | 0.0002 | 0 | 0 |
Cytoplasmic Foci | 0.009 | 0.0021 | 0.0007 | 0.0026 | 0.0009 | 0.0006 | 0.0135 | 0.001 | 0.0053 | 0.0074 | 0.0061 | 0.0004 | 0.0065 | 0.0211 | 0.0149 | 0.0068 | 0.0002 | 0.0002 |
Eisosomes | 0.0008 | 0.0003 | 0.0002 | 0.0002 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0001 | 0.0004 | 0.0002 | 0.0001 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0001 | 0.0001 |
Endoplasmic Reticulum | 0.0014 | 0.0001 | 0.0001 | 0.0008 | 0.0038 | 0.0001 | 0.002 | 0 | 0 | 0.0001 | 0 | 0.0003 | 0.0083 | 0.0001 | 0.0001 | 0.0002 | 0.0002 | 0.0002 |
Endosome | 0.0099 | 0.0053 | 0.0023 | 0.0033 | 0.0105 | 0.0049 | 0.0183 | 0.0017 | 0.0016 | 0.0056 | 0.0009 | 0.0013 | 0.084 | 0.0058 | 0.0084 | 0.0143 | 0.0009 | 0.0013 |
Golgi | 0.0092 | 0.0121 | 0.0098 | 0.0063 | 0.008 | 0.008 | 0.005 | 0.0033 | 0.0037 | 0.0126 | 0.0039 | 0.0083 | 0.0322 | 0.0075 | 0.0131 | 0.0437 | 0.0003 | 0.0025 |
Lipid Particles | 0.024 | 0.0199 | 0.0056 | 0.002 | 0.0153 | 0.0047 | 0.0469 | 0.017 | 0.012 | 0.0449 | 0.0018 | 0.0025 | 0.0361 | 0.0141 | 0.0398 | 0.0029 | 0.0005 | 0.0125 |
Mitochondria | 0.8567 | 0.919 | 0.9333 | 0.937 | 0.9267 | 0.9723 | 0.8555 | 0.9039 | 0.9411 | 0.8721 | 0.927 | 0.9744 | 0.1393 | 0.8803 | 0.8884 | 0.9055 | 0.9455 | 0.9712 |
None | 0.0043 | 0.0001 | 0 | 0.0007 | 0.0003 | 0 | 0.0077 | 0 | 0 | 0.0002 | 0 | 0.0001 | 0.0046 | 0.0002 | 0 | 0 | 0.0002 | 0 |
Nuclear Periphery | 0.0142 | 0.001 | 0.0103 | 0.0027 | 0.0182 | 0.0002 | 0.004 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.1575 | 0.0001 | 0.0001 | 0.0001 | 0.0002 | 0.0004 |
Nucleolus | 0.0028 | 0.0005 | 0.0001 | 0.0006 | 0.0002 | 0.0005 | 0.0016 | 0.0033 | 0.0001 | 0.0003 | 0 | 0.0002 | 0.0617 | 0.0005 | 0.0003 | 0 | 0.0005 | 0 |
Nucleus | 0.0184 | 0.0002 | 0.0001 | 0.0055 | 0.0009 | 0.0003 | 0.0008 | 0.0005 | 0.0001 | 0.0001 | 0 | 0.0001 | 0.2185 | 0.0002 | 0.0001 | 0.0001 | 0.0004 | 0 |
Peroxisomes | 0.0065 | 0.0053 | 0.0024 | 0.0019 | 0.0008 | 0.0012 | 0.0154 | 0.0398 | 0.0175 | 0.025 | 0.0236 | 0.0013 | 0.0011 | 0.0263 | 0.0144 | 0.0022 | 0.0005 | 0.007 |
Punctate Nuclear | 0.0082 | 0 | 0 | 0.0056 | 0.0002 | 0.0001 | 0.0009 | 0 | 0.0001 | 0.0003 | 0 | 0 | 0.1607 | 0.0011 | 0.0002 | 0.0001 | 0 | 0 |
Vacuole | 0.0027 | 0.0057 | 0.0019 | 0.0009 | 0.0043 | 0.0014 | 0.0036 | 0.0112 | 0.0004 | 0.0018 | 0.0002 | 0.0002 | 0.0306 | 0.0009 | 0.0023 | 0.0007 | 0.0039 | 0.0005 |
Vacuole Periphery | 0.009 | 0.0149 | 0.0093 | 0.004 | 0.0083 | 0.004 | 0.0038 | 0.016 | 0.0012 | 0.0081 | 0.0007 | 0.0015 | 0.024 | 0.0011 | 0.0076 | 0.0008 | 0.005 | 0.0031 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 93.5721 | 72.485 | 79.8199 | 86.0914 | 62.0268 | 90.4407 | 85.9686 | 93.7866 | 98.4797 | 82.4413 |
Translational Efficiency | 1.008 | 1.0132 | 0.9415 | 0.9483 | 0.9995 | 1.1568 | 0.9788 | 0.9435 | 0.9437 | 0.9362 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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