Standard name | |
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Description | ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 1 | 2 | 4 | 4 | 2 | 0 | 1 | 0 | 11 | 14 | 9 | 5 | 11 | 13 |
Bud | 12 | 12 | 12 | 7 | 12 | 12 | 12 | 17 | – | 18 | 12 | 9 | 4 | 4 | 1 | 19 | 6 | 7 | 3 | 8 | 2 | 4 | 2 | 1 |
Bud Neck | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | – | 1 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 1 | 3 | 0 | 2 | 5 | 2 |
Bud Site | 1 | 1 | 4 | 0 | 1 | 5 | 4 | 6 | – | 6 | 7 | 4 | 0 | 0 | 0 | 6 | 6 | 1 | – | – | – | – | – | – |
Cell Periphery | 8 | 5 | 4 | 2 | 2 | 0 | 4 | 1 | – | 2 | 5 | 3 | 3 | 3 | 3 | 9 | 14 | 9 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 2 | 4 | 10 | 6 | 8 | 8 | 3 | 7 | – | 3 | 2 | 1 | 6 | 6 | 11 | 17 | 22 | 8 | 0 | 2 | 0 | 1 | 0 | 0 |
Endoplasmic Reticulum | 0 | 2 | 1 | 0 | 1 | 1 | 1 | 1 | – | 4 | 1 | 3 | 0 | 0 | 1 | 4 | 2 | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | 48 | 6 | 24 | 3 | 14 | 3 | 7 | 6 | – | 1 | 0 | 2 | 20 | 19 | 15 | 15 | 17 | 11 | 28 | 30 | 20 | 16 | 29 | 23 |
Golgi | 5 | 0 | 1 | 2 | 5 | 1 | 0 | 2 | – | 0 | 0 | 2 | 55 | 55 | 72 | 4 | 6 | 11 | 27 | 24 | 21 | 18 | 24 | 41 |
Mitochondria | 370 | 220 | 240 | 200 | 218 | 351 | 429 | 543 | – | 465 | 342 | 428 | 133 | 133 | 148 | 17 | 12 | 11 | 384 | 159 | 215 | 163 | 162 | 195 |
Nucleus | 2 | 3 | 1 | 1 | 0 | 2 | 0 | 4 | – | 0 | 1 | 1 | 2 | 2 | 1 | 3 | 9 | 4 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | 16 | 10 | 11 | 6 | 14 | 26 | 37 | 34 | – | 43 | 36 | 46 | 5 | 6 | 5 | 15 | 7 | 8 | 1 | 0 | 2 | 1 | 3 | 3 |
Nucleolus | 55 | 20 | 19 | 11 | 32 | 52 | 73 | 73 | – | 64 | 30 | 57 | 8 | 8 | 6 | 24 | 14 | 17 | 4 | 3 | 2 | 2 | 3 | 2 |
Peroxisomes | 66 | 38 | 119 | 49 | 36 | 24 | 31 | 20 | – | 11 | 6 | 14 | 16 | 13 | 22 | 68 | 35 | 40 | 37 | 26 | 71 | 34 | 44 | 50 |
SpindlePole | 18 | 8 | 28 | 10 | 8 | 8 | 8 | 17 | – | 4 | 7 | 10 | 13 | 13 | 4 | 22 | 15 | 9 | 17 | 11 | 12 | 13 | 12 | 12 |
Vac/Vac Membrane | 57 | 23 | 10 | 6 | 8 | 12 | 12 | 11 | – | 18 | 5 | 6 | 42 | 43 | 47 | 26 | 12 | 19 | 12 | 11 | 3 | 5 | 3 | 5 |
Unique Cell Count | 491 | 271 | 345 | 245 | 279 | 409 | 492 | 618 | 505 | 359 | 466 | 214 | 215 | 247 | 198 | 141 | 132 | 542 | 301 | 371 | 274 | 307 | 361 | |
Labelled Cell Count | 661 | 354 | 484 | 303 | 359 | 505 | 621 | 742 | 641 | 455 | 588 | 312 | 310 | 338 | 249 | 178 | 156 | 542 | 301 | 371 | 274 | 307 | 361 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
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Mean Cell GFP Intensity (1e-4) | 10.0 | 9.5 | 8.5 | 9.2 | 10.1 | 9.6 | 9.3 | 8.6 | – | 8.1 | 7.7 | 8.9 | 10.5 | 10.5 | 10.7 | 8.0 | 8.6 | 11.2 | 10.2 | 11.2 | 11.6 |
Std Deviation (1e-4) | 1.9 | 2.0 | 2.1 | 3.2 | 2.7 | 2.9 | 3.3 | 2.6 | – | 2.6 | 2.4 | 3.0 | 1.9 | 1.9 | 2.3 | 1.9 | 3.0 | 3.2 | 2.6 | 2.6 | 2.3 |
Intensity Change (Log2) | – | – | – | 0.12 | 0.24 | 0.17 | 0.13 | 0.02 | – | -0.06 | -0.14 | 0.07 | 0.31 | 0.31 | 0.34 | -0.08 | 0.02 | 0.4 | 0.27 | 0.4 | 0.45 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
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Cortical Patches | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | -0.4 | 0.5 | -0.4 | -0.9 | -0.6 | – | 0.1 | -0.1 | -1.4 | -1.1 | -1.1 | -2.5 | 3.0 | 0.4 | 0.9 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | – | 0 | 0.8 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 4.6 | 0 |
Cytoplasm | -0.3 | 0 | -0.8 | -2.6 | -2.0 | – | -2.7 | -2.4 | 0 | -0.1 | -0.1 | 1.0 | 2.9 | 5.1 | 0 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Endosome | -3.3 | -1.0 | -4.6 | -4.2 | -5.1 | – | -5.7 | -5.1 | -5.2 | 1.0 | 0.8 | -0.4 | 0.3 | 1.8 | 0.5 |
Golgi | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 9.7 | 9.7 | 10.5 | 0 | 0 | 0 |
Mitochondria | 3.3 | 2.4 | 5.4 | 6.3 | 7.0 | – | 8.6 | 9.0 | 8.2 | -1.8 | -1.9 | -2.4 | -13.7 | -12.2 | -12.0 |
Nucleus | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nuclear Periphery | -0.5 | 1.2 | 2.0 | 2.7 | 1.6 | – | 3.1 | 3.6 | 3.7 | -0.6 | -0.3 | -0.9 | 2.3 | 0.9 | 1.4 |
Nucleolus | -0.6 | 2.7 | 3.4 | 4.2 | 3.2 | – | 3.5 | 1.5 | 3.2 | -0.9 | -1.0 | -1.8 | 2.7 | 1.8 | 2.7 |
Peroxisomes | -3.8 | -6.2 | -10.0 | -10.5 | -13.2 | – | -12.9 | -11.4 | -12.0 | -7.3 | -7.7 | -7.2 | 0 | -2.1 | -0.9 |
SpindlePole | -2.0 | -2.8 | -4.0 | -4.6 | -3.8 | – | -5.5 | -3.8 | -4.0 | -0.9 | -0.9 | -3.4 | 1.2 | 0.9 | -0.5 |
Vacuole | -0.3 | 0 | 0 | -0.4 | -1.1 | – | 0.5 | -1.4 | -1.6 | 6.6 | 6.7 | 6.6 | 4.6 | 2.7 | 4.7 |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
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37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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