Standard name
Description ATP-dependent Lon protease; involved in degradation of misfolded proteins in mitochondria; required for biogenesis and maintenance of mitochondria
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0
Bud 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.1 0 0.05 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.05 0.1 0.07 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0.09 0.16 0.06 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0.1 0 0.07 0 0.05 0 0 0 – 0 0 0 0.09 0.09 0.06 0.08 0.12 0.08 0.05 0.1 0.05 0.06 0.09 0.07
Golgi 0 0 0 0 0 0 0 0 – 0 0 0 0.26 0.26 0.29 0 0 0.08 0.05 0.08 0.06 0.07 0.08 0.12
Mitochondria 0.75 0.81 0.7 0.82 0.78 0.86 0.87 0.88 – 0.92 0.95 0.92 0.62 0.62 0.6 0.09 0.09 0.08 0.71 0.53 0.58 0.6 0.53 0.54
Nucleus 0 0 0 0 0 0 0 0 – 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0.05 0.06 0.08 0.06 – 0.09 0.1 0.1 0 0 0 0.08 0.05 0.06 0 0 0 0 0 0
Nucleolus 0.11 0.07 0.06 0 0.11 0.13 0.15 0.12 – 0.13 0.08 0.12 0 0 0 0.12 0.1 0.13 0 0 0 0 0 0
Peroxisomes 0.13 0.14 0.34 0.2 0.13 0.06 0.06 0 – 0 0 0 0.07 0.06 0.09 0.34 0.25 0.3 0.07 0.09 0.19 0.13 0.15 0.14
SpindlePole 0 0 0.08 0 0 0 0 0 – 0 0 0 0.06 0.06 0 0.11 0.11 0.07 0 0 0 0.05 0 0
Vac/Vac Membrane 0.12 0.08 0 0 0 0 0 0 – 0 0 0 0.2 0.2 0.19 0.13 0.09 0.14 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 – 1 1 2 4 4 2 0 1 0 11 14 9 5 11 13
Bud 12 12 12 7 12 12 12 17 – 18 12 9 4 4 1 19 6 7 3 8 2 4 2 1
Bud Neck 1 1 0 0 0 0 0 0 – 1 0 0 1 1 0 0 0 0 1 3 0 2 5 2
Bud Site 1 1 4 0 1 5 4 6 – 6 7 4 0 0 0 6 6 1 – – – – – –
Cell Periphery 8 5 4 2 2 0 4 1 – 2 5 3 3 3 3 9 14 9 0 0 0 0 0 0
Cytoplasm 2 4 10 6 8 8 3 7 – 3 2 1 6 6 11 17 22 8 0 2 0 1 0 0
Endoplasmic Reticulum 0 2 1 0 1 1 1 1 – 4 1 3 0 0 1 4 2 1 0 0 0 0 0 0
Endosome 48 6 24 3 14 3 7 6 – 1 0 2 20 19 15 15 17 11 28 30 20 16 29 23
Golgi 5 0 1 2 5 1 0 2 – 0 0 2 55 55 72 4 6 11 27 24 21 18 24 41
Mitochondria 370 220 240 200 218 351 429 543 – 465 342 428 133 133 148 17 12 11 384 159 215 163 162 195
Nucleus 2 3 1 1 0 2 0 4 – 0 1 1 2 2 1 3 9 4 0 0 0 0 0 0
Nuclear Periphery 16 10 11 6 14 26 37 34 – 43 36 46 5 6 5 15 7 8 1 0 2 1 3 3
Nucleolus 55 20 19 11 32 52 73 73 – 64 30 57 8 8 6 24 14 17 4 3 2 2 3 2
Peroxisomes 66 38 119 49 36 24 31 20 – 11 6 14 16 13 22 68 35 40 37 26 71 34 44 50
SpindlePole 18 8 28 10 8 8 8 17 – 4 7 10 13 13 4 22 15 9 17 11 12 13 12 12
Vac/Vac Membrane 57 23 10 6 8 12 12 11 – 18 5 6 42 43 47 26 12 19 12 11 3 5 3 5
Unique Cell Count 491 271 345 245 279 409 492 618 505 359 466 214 215 247 198 141 132 542 301 371 274 307 361
Labelled Cell Count 661 354 484 303 359 505 621 742 641 455 588 312 310 338 249 178 156 542 301 371 274 307 361


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 9.5 8.5 9.2 10.1 9.6 9.3 8.6 – 8.1 7.7 8.9 10.5 10.5 10.7 8.0 8.6 11.2 10.2 11.2 11.6
Std Deviation (1e-4) 1.9 2.0 2.1 3.2 2.7 2.9 3.3 2.6 – 2.6 2.4 3.0 1.9 1.9 2.3 1.9 3.0 3.2 2.6 2.6 2.3
Intensity Change (Log2) – – – 0.12 0.24 0.17 0.13 0.02 – -0.06 -0.14 0.07 0.31 0.31 0.34 -0.08 0.02 0.4 0.27 0.4 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud -0.4 0.5 -0.4 -0.9 -0.6 – 0.1 -0.1 -1.4 -1.1 -1.1 -2.5 3.0 0.4 0.9
Bud Neck 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 – 0 0.8 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 – 0 0 0 0 0 0 0 4.6 0
Cytoplasm -0.3 0 -0.8 -2.6 -2.0 – -2.7 -2.4 0 -0.1 -0.1 1.0 2.9 5.1 0
Endoplasmic Reticulum 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Endosome -3.3 -1.0 -4.6 -4.2 -5.1 – -5.7 -5.1 -5.2 1.0 0.8 -0.4 0.3 1.8 0.5
Golgi 0 0 0 0 0 – 0 0 0 9.7 9.7 10.5 0 0 0
Mitochondria 3.3 2.4 5.4 6.3 7.0 – 8.6 9.0 8.2 -1.8 -1.9 -2.4 -13.7 -12.2 -12.0
Nucleus 0 0 0 0 0 – 0 0 0 0 0 0 0 0 0
Nuclear Periphery -0.5 1.2 2.0 2.7 1.6 – 3.1 3.6 3.7 -0.6 -0.3 -0.9 2.3 0.9 1.4
Nucleolus -0.6 2.7 3.4 4.2 3.2 – 3.5 1.5 3.2 -0.9 -1.0 -1.8 2.7 1.8 2.7
Peroxisomes -3.8 -6.2 -10.0 -10.5 -13.2 – -12.9 -11.4 -12.0 -7.3 -7.7 -7.2 0 -2.1 -0.9
SpindlePole -2.0 -2.8 -4.0 -4.6 -3.8 – -5.5 -3.8 -4.0 -0.9 -0.9 -3.4 1.2 0.9 -0.5
Vacuole -0.3 0 0 -0.4 -1.1 – 0.5 -1.4 -1.6 6.6 6.7 6.6 4.6 2.7 4.7