Standard name
Human Ortholog
Description Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.1 0.16 0.16 0.13 0.17 0.11 0.15 0.1 0.15 0.08 0.28 0.27 0.11 0.11 0.09 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.1 0 0.05 0 0 0 0 0.07 0.07 0.08 0 0 0 0.09 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.19 0.34 0.08 0.18 0.07 0.41 0.36 0.51 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.61 0.62 0.5 0.56 0.58 0.62 0.56 0.59 0.62 0.51 0.64 0.46 0.53 0.81 0.83 0.86 0.8 0.63 0.58 0.52 0.51 0.46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0.06 0.06 0.08 0.1 0.07 0.13 0.12 0 0 0 0 0 0 0 0 0 0 0.06 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0.22 0.21 0.1 0.1 0.11 0.12 0.06 0 0.06 0.05 0.25 0.21 0.13 0 0 0 0 0.13 0.18 0.22 0.22 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 3 1 1 1
Bud 0 1 2 0 0 0 0 1 0 0 0 0 0 1 0 0 0 2 4 2 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 1 1
Bud Site 0 0 0 1 0 1 2 4 2 2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 26 24 29 20 30 18 25 6 18 11 40 49 27 23 20 0 3 6 9 4 8
Endoplasmic Reticulum 0 6 3 2 1 1 1 0 0 0 1 4 1 2 1 5 0 1 5 2 2 2
Endosome 3 10 2 17 6 8 2 1 0 2 10 10 14 4 1 1 0 1 13 4 5 11
Golgi 0 0 0 0 0 3 0 0 0 0 0 0 1 1 0 2 0 1 3 1 3 4
Mitochondria 3 53 50 14 29 13 70 61 32 53 4 1 1 5 4 7 0 0 8 1 2 3
Nucleus 41 168 73 100 91 109 94 99 39 61 86 65 97 198 171 201 7 54 183 98 84 103
Nuclear Periphery 0 0 2 0 2 0 1 3 0 0 1 1 0 0 2 2 0 1 3 2 1 1
Nucleolus 0 9 7 10 10 14 17 11 8 14 4 3 2 4 4 6 0 0 5 8 9 18
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 3 1 0 0 0 0 0 1 2 1 1 0 2 9 9 3 5
Vac/Vac Membrane 15 56 14 18 17 21 10 6 4 6 34 30 24 11 7 5 0 11 58 41 36 50
Unique Cell Count 67 273 147 177 158 175 169 169 63 119 134 141 183 245 206 235 10 86 319 188 164 224
Labelled Cell Count 70 329 179 192 176 203 216 211 91 156 151 155 191 256 214 250 10 86 319 188 164 224


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.2 5.9 6.2 5.5 9.3 5.4 5.9 5.1 5.6 6.4 7.5 7.3 8.7 8.7 8.3 7.0 6.7 7.1
Std Deviation (1e-4) 1.3 2.1 3.7 2.2 2.4 9.4 3.0 2.9 1.6 2.7 1.5 3.2 3.7 1.9 1.9 2.1 2.0 2.2 4.1
Intensity Change (Log2) 0.18 0.25 0.08 0.84 0.05 0.19 -0.02 0.12 0.29 0.52 0.49 0.75 0.75 0.68 0.44 0.38 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 2.2 1.0 2.4 0.4 1.7 0 1.6 -0.4 5.0 4.9 0.6 0.6 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.1
Endosome 0 2.6 0.1 0.5 0 0 0 0 1.7 1.5 1.9 -1.4 0 -2.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 -3.3 -0.3 -3.5 5.0 3.9 5.2 5.1 -4.5 -5.4 -6.1 -6.3 -5.8 -5.7
Nucleus -2.3 -1.1 -0.8 0.2 -1.2 -0.6 0.1 -1.9 0.5 -3.0 -1.8 4.8 5.1 6.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.7 1.2 1.5 2.2 2.9 1.6 0 3.3 0 0 0 -1.2 -0.9 -0.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.9 -2.9 -2.6 -2.3 -4.2 -5.0 -2.6 -3.9 1.1 0.2 -2.0 -5.4 -5.5 -6.4

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 1.5224 1.5016 1.3448 0.9041 0.9996 1.2293 0.9091 0.3449 0.1821 0.4943 0.2374 0.4805 2.0826 2.0339 1.652 1.7513 1.7965 1.8863
Actin 0.0059 0.0001 0.0017 0.0023 0.0598 0.0004 0.0052 0.0002 0.0004 0.0021 0.0033 0.0066 0.0579 0.0001 0.0136 0.0047 0.004 0.0031
Bud 0.0004 0.0003 0.0002 0.0005 0.0024 0.0003 0.0004 0.0026 0.0005 0.0002 0.0009 0.0004 0.0019 0.0001 0.0009 0.0001 0.0034 0.0001
Bud Neck 0.0017 0.0005 0.0009 0.0097 0.019 0.0029 0.0211 0.0007 0.002 0.0007 0.0029 0.0102 0.0092 0.0004 0.001 0.0004 0.0034 0.0034
Bud Periphery 0.0005 0.0002 0.0002 0.0011 0.0025 0.0008 0.0005 0.0004 0.0008 0.0001 0.001 0.0008 0.0048 0 0.0015 0.0001 0.0039 0.0002
Bud Site 0.0019 0.0061 0.0016 0.0006 0.008 0.0003 0.0091 0.0066 0.003 0.0007 0.0028 0.0016 0.0097 0.0005 0.0061 0.0006 0.0159 0.0001
Cell Periphery 0.0003 0.0001 0.0001 0.0002 0.0003 0.0001 0.0005 0.0001 0.0002 0 0.0003 0.0002 0.0011 0 0.0002 0 0.0006 0
Cytoplasm 0.0254 0.0291 0.0276 0.0113 0.0307 0.0084 0.0356 0.0372 0.0213 0.006 0.0202 0.0073 0.0185 0.0332 0.0219 0.0145 0.0817 0.0128
Cytoplasmic Foci 0.0228 0.0022 0.0097 0.0028 0.0168 0.0015 0.0586 0.0007 0.0128 0.0178 0.0234 0.0096 0.0395 0.0005 0.0024 0.029 0.0048 0.0005
Eisosomes 0.0002 0 0 0.0001 0.0003 0 0.0002 0 0 0 0 0 0.0005 0 0.0001 0 0.0001 0
Endoplasmic Reticulum 0.0026 0.0006 0.0013 0.0043 0.0046 0.0005 0.0055 0.0013 0.0009 0.0003 0.0009 0.0006 0.0098 0.0009 0.003 0.0002 0.0079 0.0013
Endosome 0.0446 0.002 0.0073 0.0321 0.0427 0.0043 0.0575 0.0013 0.0174 0.0662 0.052 0.0073 0.0442 0.0008 0.0102 0.0029 0.0223 0.0026
Golgi 0.0121 0.0009 0.0041 0.004 0.014 0.0006 0.018 0.0001 0.002 0.0142 0.048 0.0024 0.0092 0 0.0026 0.0046 0.0048 0.0011
Lipid Particles 0.0222 0.0001 0.0028 0.0007 0.0319 0.0005 0.0453 0.0001 0.0127 0.0011 0.0383 0.0125 0.02 0.0001 0.0147 0.0054 0.0014 0.0006
Mitochondria 0.0081 0.0003 0.0086 0.0179 0.0172 0.002 0.0144 0.0118 0.0015 0.0013 0.0161 0.008 0.0384 0.0004 0.0019 0.0007 0.0031 0.0007
None 0.0132 0.014 0.0177 0.0148 0.0147 0.0025 0.0554 0.0487 0.0689 0.0006 0.0087 0.0117 0.0571 0.0374 0.0718 0.0165 0.0861 0.0275
Nuclear Periphery 0.0145 0.0066 0.01 0.0161 0.0115 0.0057 0.0182 0.0172 0.0044 0.0104 0.0095 0.0094 0.024 0.009 0.0071 0.0054 0.0306 0.0122
Nucleolus 0.0219 0.0076 0.0062 0.0056 0.0065 0.0098 0.0081 0.0084 0.0218 0.0045 0.007 0.0244 0.0059 0.0111 0.0061 0.003 0.0053 0.0134
Nucleus 0.7708 0.9123 0.8743 0.8496 0.6749 0.9251 0.5636 0.8438 0.7987 0.8288 0.7184 0.861 0.589 0.8913 0.8081 0.8478 0.688 0.892
Peroxisomes 0.015 0.0001 0.0016 0.0003 0.0139 0.0001 0.0176 0.0004 0.0015 0.0076 0.0059 0.0024 0.0243 0 0.0062 0.0224 0.0008 0.0001
Punctate Nuclear 0.0112 0.0156 0.0224 0.0093 0.0202 0.0292 0.0543 0.0157 0.0272 0.0345 0.036 0.0199 0.0227 0.0128 0.0178 0.0416 0.012 0.0271
Vacuole 0.0036 0.0012 0.0013 0.0136 0.0063 0.004 0.0068 0.0022 0.0017 0.0019 0.0032 0.0027 0.0096 0.0011 0.0018 0.0001 0.0179 0.0006
Vacuole Periphery 0.0012 0.0002 0.0003 0.0031 0.0016 0.0008 0.004 0.0005 0.0005 0.0009 0.0012 0.0009 0.0027 0.0002 0.0009 0.0001 0.002 0.0004

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 12.6828 15.8727 6.7304 10.7107 18.0882 20.2813 12.6098 9.2845 17.3664 19.0147
Translational Efficiency 2.1652 1.4203 2.597 1.6948 1.4795 1.9695 1.795 2.8632 1.3662 1.6435

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1207 1053 1002 732 144 413 2104 372 1351 1466 3106 1104

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 680.92 763.77 874.34 858.83 712.78 736.31 897.15 912.23 684.32 756.03 889.79 876.82
Standard Deviation 112.52 134.74 132.20 170.91 110.20 122.71 152.87 149.52 112.70 132.04 146.91 165.95
Intensity Change Log 2 0.165653 0.360709 0.334887 0.046856 0.331892 0.355941 0.106119 0.346043 0.345693

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000367 0.001190 0.001660 0.006398 0.000732 0.000652 0.002382 0.010156 0.000406 0.001038 0.002149 0.007664
Bud Neck 0.006986 0.015253 0.021241 0.016887 0.003639 0.012388 0.068606 0.025146 0.006629 0.014446 0.053326 0.019670
Bud Site 0.004155 0.015351 0.009804 0.036088 0.008926 0.009701 0.023598 0.033198 0.004664 0.013759 0.019148 0.035114
Cell Periphery 0.000233 0.000353 0.000160 0.000508 0.001715 0.000331 0.000315 0.000510 0.000391 0.000347 0.000265 0.000508
Cytoplasm 0.140237* 0.065753 0.010131 0.063840 0.021385 0.100275* 0.021227 0.054643 0.127569* 0.075479 0.017647 0.060741
Cytoplasmic Foci 0.015871 0.011346 0.003265 0.040109 0.002176 0.029285 0.005757 0.029286 0.014411 0.016400 0.004953 0.036462
Eisosomes 0.000035 0.000036 0.000029 0.000068 0.000046 0.000065 0.000041 0.000052 0.000036 0.000044 0.000037 0.000063
Endoplasmic Reticulum 0.001883 0.005897 0.006393 0.007547 0.003948 0.002190 0.009358 0.009001 0.002103 0.004853 0.008402 0.008037
Endosome 0.001254 0.006964 0.002886 0.066062 0.000497 0.013537 0.007330 0.034710 0.001174 0.008816 0.005897 0.055498
Golgi 0.001076 0.005799 0.002418 0.053736 0.000068 0.007337 0.005594 0.042243 0.000968 0.006232 0.004570 0.049863
Lipid Particles 0.003197 0.003410 0.000912 0.009472 0.001135 0.004563 0.001087 0.001241 0.002977 0.003735 0.001030 0.006698
Mitochondria 0.004166 0.018979 0.009194 0.049795 0.000865 0.013220 0.020220 0.021595 0.003814 0.017357 0.016663 0.040293
Mitotic Spindle 0.006583 0.029757 0.014668 0.116175* 0.000130 0.010465 0.040569 0.072825 0.005895 0.024322 0.032213 0.101568*
None 0.040074 0.029925 0.009273 0.029800 0.005923 0.030829 0.007309 0.030974 0.036434 0.030180 0.007943 0.030195
Nuclear Periphery 0.001230 0.003460 0.001150 0.003159 0.000852 0.000958 0.003805 0.002439 0.001190 0.002755 0.002949 0.002917
Nuclear Periphery Foci 0.000797 0.002989 0.000590 0.006855 0.000272 0.000843 0.001995 0.002720 0.000741 0.002384 0.001542 0.005462
Nucleolus 0.008178 0.012632 0.004520 0.005810 0.011588 0.019706 0.004361 0.002905 0.008541 0.014625 0.004412 0.004831
Nucleus 0.756100* 0.747674* 0.890444* 0.413105* 0.933230* 0.718220* 0.757807* 0.588140* 0.774980* 0.739376* 0.800596* 0.472084*
Peroxisomes 0.001986 0.004235 0.002037 0.005395 0.000437 0.009285 0.002685 0.010695 0.001821 0.005658 0.002476 0.007181
Vacuole 0.005030 0.014990 0.007628 0.045687 0.002295 0.012308 0.014194 0.022607 0.004739 0.014234 0.012076 0.037910
Vacuole Periphery 0.000563 0.004005 0.001596 0.023505 0.000141 0.003843 0.001759 0.004914 0.000518 0.003959 0.001706 0.017241

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.84 -4.66 -3.36 -2.54 -1.99 0.40 -3.77 -2.87 -3.03 -1.89 -2.77 -7.83 -4.52 -3.73 -2.53
Bud Neck -3.87 -5.65 -5.67 -1.26 1.52 -2.82 -19.61 -6.56 -3.36 8.68 -4.16 -18.02 -7.54 -3.19 11.63
Bud Site -4.38 -2.83 -6.08 -1.97 -4.08 -0.15 -1.84 -1.50 -2.27 0.24 -4.41 -7.94 -6.84 -2.68 -1.88
Cell Periphery -0.79 2.45 -1.73 0.14 -4.02 0.93 0.98 0.97 0.63 -0.14 0.37 1.00 0.59 0.33 -2.16
Cytoplasm 9.40 20.90 9.20 0.40 -10.39 -7.52 0.25 -2.34 6.15 -3.92 6.84 19.49 10.19 3.82 -10.11
Cytoplasmic Foci 2.72 7.61 0.49 -1.92 -6.13 -6.57 -1.14 -2.28 4.40 -1.86 -0.45 7.26 0.93 1.41 -5.94
Eisosomes -0.01 1.89 -8.15 -8.60 -11.05 -1.88 0.85 -1.25 1.13 -5.00 -2.37 0.65 -8.06 -6.06 -11.24
Endoplasmic Reticulum -6.86 -8.28 -13.02 -5.69 -4.69 2.44 -6.15 -5.55 -7.68 -1.95 -5.99 -13.68 -13.90 -8.80 -3.42
Endosome -5.50 -1.31 -8.41 -6.40 -7.99 -4.78 -5.95 -3.70 0.37 -2.12 -7.06 -5.10 -9.04 -5.41 -7.59
Golgi -3.20 -0.70 -6.06 -4.55 -5.74 -3.01 -4.48 -3.17 -1.36 -2.25 -4.10 -2.92 -6.70 -4.58 -5.82
Lipid Particles 0.14 3.01 -0.49 -0.58 -2.25 -3.26 0.68 -0.26 3.45 -1.70 -0.55 3.40 -0.05 0.40 -2.51
Mitochondria -5.28 -2.17 -5.52 -1.31 -4.16 -3.31 -9.34 -3.48 0.50 1.95 -5.81 -6.79 -5.97 -0.81 -1.92
Mitotic Spindle -5.05 -2.60 -9.56 -6.30 -8.11 -3.29 -11.59 -5.79 -4.25 -1.62 -5.21 -8.44 -10.74 -7.34 -6.70
None 2.10 8.04 4.58 2.31 -3.37 -4.91 -0.29 -2.52 2.54 -2.54 1.43 8.81 4.65 3.31 -4.69
Nuclear Periphery -2.42 0.34 -7.61 -0.47 -7.19 -0.72 -5.22 -6.99 -6.88 -0.24 -2.34 -4.31 -9.47 -1.64 -2.27
Nuclear Periphery Foci -3.44 2.00 -3.40 -0.96 -3.89 -2.18 -3.17 -3.34 -1.45 -0.67 -3.59 -1.30 -3.94 -1.16 -3.14
Nucleolus -2.12 2.92 2.27 4.09 -0.92 -1.53 2.39 2.52 4.02 0.69 -3.06 4.15 3.45 5.81 -0.92
Nucleus 0.49 -12.10 9.46 8.53 18.70 10.24 9.73 9.81 -0.36 3.66 3.06 -4.61 9.88 6.90 14.73
Peroxisomes -2.06 0.86 -0.22 1.73 -0.96 -3.44 -2.69 -1.97 1.90 -1.00 -3.50 0.36 -0.96 2.45 -1.25
Vacuole -4.68 -1.38 -11.47 -8.17 -10.97 -4.94 -9.63 -7.16 -4.69 -5.25 -5.71 -5.39 -13.07 -9.30 -11.42
Vacuole Periphery -3.38 -1.43 -5.00 -2.97 -4.55 -2.21 -5.87 -2.60 1.12 -0.66 -3.91 -2.78 -5.24 -2.31 -4.62