Standard name
Description Subunit of the Hap2p/3p/4p/5p CCAAT-binding complex; complex is heme-activated and glucose-repressed; complex is a transcriptional activator and global regulator of respiratory gene expression; contains sequences contributing to both complex assembly and DNA binding

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.12 0.1 0.16 0.16 0.13 0.17 0.11 0.15 0.1 0.15 0.08 0.28 0.27 0.11 0.11 0.09 0 0 0 0.05 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0.1 0 0.05 0 0 0 0 0.07 0.07 0.08 0 0 0 0.09 0 0 0 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.19 0.34 0.08 0.18 0.07 0.41 0.36 0.51 0.45 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0.61 0.62 0.5 0.56 0.58 0.62 0.56 0.59 0.62 0.51 0.64 0.46 0.53 0.81 0.83 0.86 0.8 0.63 0.58 0.52 0.51 0.46
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0.05 0.06 0.06 0.08 0.1 0.07 0.13 0.12 0 0 0 0 0 0 0 0 0 0 0.06 0.08
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0
Vac/Vac Membrane 0.22 0.21 0.1 0.1 0.11 0.12 0.06 0 0.06 0.05 0.25 0.21 0.13 0 0 0 0 0.13 0.18 0.22 0.22 0.23
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 1 3 1 1 1
Bud 0 1 2 0 0 0 0 1 0 0 0 0 0 1 0 0 0 2 4 2 1 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 3 1 1
Bud Site 0 0 0 1 0 1 2 4 2 2 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 8 26 24 29 20 30 18 25 6 18 11 40 49 27 23 20 0 3 6 9 4 8
Endoplasmic Reticulum 0 6 3 2 1 1 1 0 0 0 1 4 1 2 1 5 0 1 5 2 2 2
Endosome 3 10 2 17 6 8 2 1 0 2 10 10 14 4 1 1 0 1 13 4 5 11
Golgi 0 0 0 0 0 3 0 0 0 0 0 0 1 1 0 2 0 1 3 1 3 4
Mitochondria 3 53 50 14 29 13 70 61 32 53 4 1 1 5 4 7 0 0 8 1 2 3
Nucleus 41 168 73 100 91 109 94 99 39 61 86 65 97 198 171 201 7 54 183 98 84 103
Nuclear Periphery 0 0 2 0 2 0 1 3 0 0 1 1 0 0 2 2 0 1 3 2 1 1
Nucleolus 0 9 7 10 10 14 17 11 8 14 4 3 2 4 4 6 0 0 5 8 9 18
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 1 1 0 3 1 0 0 0 0 0 1 2 1 1 0 2 9 9 3 5
Vac/Vac Membrane 15 56 14 18 17 21 10 6 4 6 34 30 24 11 7 5 0 11 58 41 36 50
Unique Cell Count 67 273 147 177 158 175 169 169 63 119 134 141 183 245 206 235 10 86 319 188 164 224
Labelled Cell Count 70 329 179 192 176 203 216 211 91 156 151 155 191 256 214 250 10 86 319 188 164 224


Cytoplasm, Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 6.3 5.2 5.9 6.2 5.5 9.3 5.4 5.9 5.1 5.6 6.4 7.5 7.3 8.7 8.7 8.3 7.0 6.7 7.1
Std Deviation (1e-4) 1.3 2.1 3.7 2.2 2.4 9.4 3.0 2.9 1.6 2.7 1.5 3.2 3.7 1.9 1.9 2.1 2.0 2.2 4.1
Intensity Change (Log2) 0.18 0.25 0.08 0.84 0.05 0.19 -0.02 0.12 0.29 0.52 0.49 0.75 0.75 0.68 0.44 0.38 0.45


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.1 2.2 1.0 2.4 0.4 1.7 0 1.6 -0.4 5.0 4.9 0.6 0.6 -0.4
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 -0.1
Endosome 0 2.6 0.1 0.5 0 0 0 0 1.7 1.5 1.9 -1.4 0 -2.5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 -3.3 -0.3 -3.5 5.0 3.9 5.2 5.1 -4.5 -5.4 -6.1 -6.3 -5.8 -5.7
Nucleus -2.3 -1.1 -0.8 0.2 -1.2 -0.6 0.1 -1.9 0.5 -3.0 -1.8 4.8 5.1 6.0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.7 1.2 1.5 2.2 2.9 1.6 0 3.3 0 0 0 -1.2 -0.9 -0.5
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.9 -2.9 -2.6 -2.3 -4.2 -5.0 -2.6 -3.9 1.1 0.2 -2.0 -5.4 -5.5 -6.4


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Hap3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available