Standard name
Description Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.18 0.06 0 0 0 0 0 0.05 0.05 0.06 0 0.07 0.1 0 – – – 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.07 0 0.07 0 0.06 0 0 0 – – – 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.07 0.07 0 0.06 0.05 0.06 0.07 0.07 0 0 0.05 – – – 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.11 0 0 0 0.06 0 0 0 0.05 0 0 0.06 0 0 – – – 0 0 0 0 0 0
Endosome 0.21 0.11 0.37 0.52 0.4 0.2 0.22 0.2 0.12 0.07 0.07 0.12 0.2 0.16 0.14 – – – 0.45 0.41 0.44 0.42 0.3 0.29
Golgi 0.51 0.51 0.49 0.16 0.3 0.13 0.07 0.12 0.17 0.1 0.14 0.08 0.62 0.63 0.67 – – – 0.45 0.44 0.48 0.45 0.59 0.58
Mitochondria 0.17 0.4 0.2 0.3 0.22 0.61 0.68 0.59 0.75 0.75 0.76 0.73 0.08 0.08 0.08 – – – 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Nucleolus 0 0 0 0.12 0.07 0.06 0.05 0 0.05 0.05 0 0.05 0 0 0 – – – 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Vac/Vac Membrane 0.34 0.21 0.13 0.14 0.19 0.09 0.08 0.09 0.06 0 0.08 0.06 0.18 0.22 0.23 – – – 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 26 46 17 4 5 6 5 17 14 16 14 8 19 23 12 – – – 8 9 5 18 18 23
Bud 1 2 5 4 0 10 18 33 12 23 9 17 4 4 2 – – – 2 0 0 2 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 3 1 0 1 0 0 0 – – – – – – – – –
Cell Periphery 1 0 0 0 0 0 1 1 0 2 0 3 0 2 1 – – – 0 0 0 0 0 0
Cytoplasm 13 10 12 14 15 22 15 28 14 19 18 19 8 10 14 – – – 0 1 1 1 2 2
Endoplasmic Reticulum 15 28 5 2 4 19 3 16 12 17 10 10 16 9 9 – – – 1 2 0 4 5 6
Endosome 91 28 101 103 91 60 83 93 35 22 18 32 53 36 43 – – – 217 125 133 229 170 187
Golgi 215 131 133 32 68 38 27 56 50 33 34 22 161 142 200 – – – 218 133 147 245 339 373
Mitochondria 73 103 55 59 50 184 254 269 216 247 190 192 22 19 25 – – – 10 7 1 21 14 16
Nucleus 1 0 0 0 0 1 0 0 1 0 1 1 0 1 0 – – – 0 1 1 0 0 0
Nuclear Periphery 1 1 5 0 1 2 2 4 2 5 2 2 2 5 0 – – – 0 1 1 0 2 0
Nucleolus 15 7 4 24 15 19 18 20 14 18 6 14 7 6 9 – – – 0 0 0 0 0 0
Peroxisomes 0 0 6 5 1 1 7 7 3 5 3 1 1 2 0 – – – 2 1 7 11 6 7
SpindlePole 5 0 5 9 4 3 9 7 6 12 1 5 4 2 3 – – – 1 1 1 2 0 1
Vac/Vac Membrane 143 54 35 27 42 27 30 39 18 10 20 15 48 49 70 – – – 17 16 2 8 9 13
Unique Cell Count 425 258 273 197 225 301 373 454 289 328 251 262 260 226 299 484 304 306 548 571 640
Labelled Cell Count 600 410 383 283 296 392 473 592 400 430 326 342 345 310 388 484 304 306 548 571 640


Endosome, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.7 13.1 11.3 10.9 10.3 9.2 8.7 8.9 8.5 7.9 8.2 7.8 12.5 12.6 12.9 – – – 11.5 13.3 14.3
Std Deviation (1e-4) 2.0 2.3 1.9 2.0 1.8 1.5 1.6 1.6 1.8 1.6 1.6 1.5 2.0 2.3 2.3 – – – 2.4 2.8 3.4
Intensity Change (Log2) – – – -0.06 -0.13 -0.3 -0.39 -0.35 -0.41 -0.52 -0.46 -0.54 0.15 0.15 0.18 – – – 0.02 0.23 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.2 -2.2 -2.6 -3.4 -1.5 -0.7 -0.7 -0.3 -1.7 0.5 1.6 -1.2 – – –
Bud 0 0 1.1 2.0 3.2 1.6 3.0 1.2 2.7 0 0 0 – – –
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Cytoplasm 1.3 1.1 1.5 -0.2 1.0 0.3 0.8 1.4 1.4 -0.8 0 0.2 – – –
Endoplasmic Reticulum 0 0 2.7 0 1.3 1.6 2.2 1.5 1.4 2.6 1.4 0.9 – – –
Endosome 3.3 0.8 -4.5 -4.1 -4.9 -6.9 -9.2 -8.1 -6.6 -4.2 -5.2 -6.2 – – –
Golgi -7.3 -4.2 -9.4 -12.1 -10.8 -7.9 -10.6 -8.6 -10.3 3.1 3.2 4.4 – – –
Mitochondria 2.4 0.6 9.9 12.1 10.3 12.9 13.5 12.7 12.3 -3.8 -3.7 -4.1 – – –
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 – – –
Nucleolus 4.8 3.0 3.0 2.3 2.1 2.3 2.6 0 2.5 1.0 0 1.2 – – –
Peroxisomes 0 0 0 -0.3 0 0 0 0 0 0 0 0 – – –
SpindlePole 1.7 0 0 0.5 0 0.2 1.3 0 0 0 0 0 – – –
Vacuole 0.3 1.8 -1.5 -2.0 -1.8 -2.7 -4.5 -1.8 -2.8 1.8 2.6 3.3 – – –