Standard name
Human Ortholog
Description Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.18 0.06 0 0 0 0 0 0.05 0.05 0.06 0 0.07 0.1 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.05 0.07 0 0.07 0 0.06 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0.07 0.07 0.07 0 0.06 0.05 0.06 0.07 0.07 0 0 0.05 0 0 0 0 0 0
Endoplasmic Reticulum 0 0.11 0 0 0 0.06 0 0 0 0.05 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0.21 0.11 0.37 0.52 0.4 0.2 0.22 0.2 0.12 0.07 0.07 0.12 0.2 0.16 0.14 0.45 0.41 0.44 0.42 0.3 0.29
Golgi 0.51 0.51 0.49 0.16 0.3 0.13 0.07 0.12 0.17 0.1 0.14 0.08 0.62 0.63 0.67 0.45 0.44 0.48 0.45 0.59 0.58
Mitochondria 0.17 0.4 0.2 0.3 0.22 0.61 0.68 0.59 0.75 0.75 0.76 0.73 0.08 0.08 0.08 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0.12 0.07 0.06 0.05 0 0.05 0.05 0 0.05 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.34 0.21 0.13 0.14 0.19 0.09 0.08 0.09 0.06 0 0.08 0.06 0.18 0.22 0.23 0 0.05 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 26 46 17 4 5 6 5 17 14 16 14 8 19 23 12 8 9 5 18 18 23
Bud 1 2 5 4 0 10 18 33 12 23 9 17 4 4 2 2 0 0 2 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 1 2 3 1 0 1 0 0 0
Cell Periphery 1 0 0 0 0 0 1 1 0 2 0 3 0 2 1 0 0 0 0 0 0
Cytoplasm 13 10 12 14 15 22 15 28 14 19 18 19 8 10 14 0 1 1 1 2 2
Endoplasmic Reticulum 15 28 5 2 4 19 3 16 12 17 10 10 16 9 9 1 2 0 4 5 6
Endosome 91 28 101 103 91 60 83 93 35 22 18 32 53 36 43 217 125 133 229 170 187
Golgi 215 131 133 32 68 38 27 56 50 33 34 22 161 142 200 218 133 147 245 339 373
Mitochondria 73 103 55 59 50 184 254 269 216 247 190 192 22 19 25 10 7 1 21 14 16
Nucleus 1 0 0 0 0 1 0 0 1 0 1 1 0 1 0 0 1 1 0 0 0
Nuclear Periphery 1 1 5 0 1 2 2 4 2 5 2 2 2 5 0 0 1 1 0 2 0
Nucleolus 15 7 4 24 15 19 18 20 14 18 6 14 7 6 9 0 0 0 0 0 0
Peroxisomes 0 0 6 5 1 1 7 7 3 5 3 1 1 2 0 2 1 7 11 6 7
SpindlePole 5 0 5 9 4 3 9 7 6 12 1 5 4 2 3 1 1 1 2 0 1
Vac/Vac Membrane 143 54 35 27 42 27 30 39 18 10 20 15 48 49 70 17 16 2 8 9 13
Unique Cell Count 425 258 273 197 225 301 373 454 289 328 251 262 260 226 299 484 304 306 548 571 640
Labelled Cell Count 600 410 383 283 296 392 473 592 400 430 326 342 345 310 388 484 304 306 548 571 640


Endosome, Punctate Nuclear

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 12.7 13.1 11.3 10.9 10.3 9.2 8.7 8.9 8.5 7.9 8.2 7.8 12.5 12.6 12.9 11.5 13.3 14.3
Std Deviation (1e-4) 2.0 2.3 1.9 2.0 1.8 1.5 1.6 1.6 1.8 1.6 1.6 1.5 2.0 2.3 2.3 2.4 2.8 3.4
Intensity Change (Log2) -0.06 -0.13 -0.3 -0.39 -0.35 -0.41 -0.52 -0.46 -0.54 0.15 0.15 0.18 0.02 0.23 0.34


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches -2.2 -2.2 -2.6 -3.4 -1.5 -0.7 -0.7 -0.3 -1.7 0.5 1.6 -1.2
Bud 0 0 1.1 2.0 3.2 1.6 3.0 1.2 2.7 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.3 1.1 1.5 -0.2 1.0 0.3 0.8 1.4 1.4 -0.8 0 0.2
Endoplasmic Reticulum 0 0 2.7 0 1.3 1.6 2.2 1.5 1.4 2.6 1.4 0.9
Endosome 3.3 0.8 -4.5 -4.1 -4.9 -6.9 -9.2 -8.1 -6.6 -4.2 -5.2 -6.2
Golgi -7.3 -4.2 -9.4 -12.1 -10.8 -7.9 -10.6 -8.6 -10.3 3.1 3.2 4.4
Mitochondria 2.4 0.6 9.9 12.1 10.3 12.9 13.5 12.7 12.3 -3.8 -3.7 -4.1
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 4.8 3.0 3.0 2.3 2.1 2.3 2.6 0 2.5 1.0 0 1.2
Peroxisomes 0 0 0 -0.3 0 0 0 0 0 0 0 0
SpindlePole 1.7 0 0 0.5 0 0.2 1.3 0 0 0 0 0
Vacuole 0.3 1.8 -1.5 -2.0 -1.8 -2.7 -4.5 -1.8 -2.8 1.8 2.6 3.3

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 7.8111 10.3482 9.4416 8.7717 8.6794 9.3808 10.03 11.9234 11.4161 11.0919 10.4038 11.437 10.3809 11.6528 11.4132 11.3294 11.3148 11.6717
Actin 0.0242 0.0132 0.0272 0.0453 0.0097 0.0042 0.0288 0.0008 0.0139 0.0469 0.0452 0.0026 0.0211 0.0111 0.0517 0.0749 0.0002 0.003
Bud 0.0006 0.0004 0.0014 0.0002 0.0004 0.0001 0.0001 0.0003 0.0004 0.0007 0.0004 0 0.0001 0.0001 0.0003 0.0004 0 0
Bud Neck 0.0007 0.0004 0.0011 0.0003 0.0003 0.0002 0.0003 0.0002 0.0003 0.0044 0.0007 0.0006 0.0003 0.0002 0.0006 0.0004 0.0001 0.0002
Bud Periphery 0.0006 0.0004 0.0021 0.0002 0.0011 0.0001 0.0001 0.0002 0.0006 0.0019 0.0006 0 0.0001 0 0.0002 0.0006 0 0
Bud Site 0.0024 0.0013 0.0068 0.0006 0.0007 0.0002 0.0005 0.0004 0.0004 0.0047 0.0009 0.0001 0.0003 0.0001 0.0029 0.0003 0 0.0001
Cell Periphery 0.0002 0.0001 0.0001 0 0.0001 0 0.0001 0.0001 0.0001 0.0006 0.0001 0 0.0001 0 0.0001 0.0001 0 0
Cytoplasm 0.0039 0.0066 0.0018 0.0017 0.001 0.0012 0.0063 0.0025 0.0054 0.0031 0.0041 0.0009 0.0019 0.0013 0.0164 0.001 0.0005 0.0009
Cytoplasmic Foci 0.0691 0.0795 0.0575 0.0745 0.056 0.0427 0.0809 0.1033 0.083 0.0824 0.1381 0.0652 0.072 0.0589 0.0766 0.06 0.0764 0.0562
Eisosomes 0.0001 0 0.0002 0.0001 0.0003 0 0.0002 0 0.0001 0.0005 0.0002 0.0001 0.0001 0 0.0002 0.0003 0 0
Endoplasmic Reticulum 0.0028 0.0011 0.0021 0.0004 0.0014 0.0007 0.0014 0.0009 0.0007 0.0008 0.0019 0.0005 0.0008 0.0006 0.0017 0.0008 0.0001 0.0005
Endosome 0.2861 0.3904 0.2802 0.4096 0.3501 0.2363 0.3062 0.3664 0.2831 0.2669 0.3026 0.2188 0.2551 0.4417 0.3101 0.3138 0.3597 0.3007
Golgi 0.4453 0.4524 0.5687 0.3302 0.4497 0.6423 0.518 0.4669 0.5693 0.4946 0.3974 0.6535 0.5594 0.4488 0.5119 0.518 0.556 0.6078
Lipid Particles 0.0342 0.0205 0.009 0.0157 0.0515 0.0351 0.0304 0.0108 0.015 0.0092 0.0489 0.0321 0.0756 0.0082 0.0082 0.0104 0.004 0.0204
Mitochondria 0.046 0.0044 0.0204 0.0582 0.031 0.0088 0.0039 0.0291 0.0113 0.0298 0.0061 0.0079 0.0058 0.0041 0.0041 0.0097 0.0002 0.0021
None 0.0012 0.0001 0.0003 0.0006 0.0009 0.0006 0.0026 0.0001 0.0002 0.0051 0.0023 0.0016 0.0001 0 0.0006 0.0001 0 0
Nuclear Periphery 0.0158 0.001 0.0079 0.0005 0.0164 0.0016 0.0032 0.0003 0.0007 0.0013 0.0031 0.0007 0.0002 0.0002 0.0021 0.0002 0 0.0002
Nucleolus 0.0012 0.0001 0.0002 0.0003 0.0023 0.0001 0.0001 0.0002 0.0001 0.0105 0.0004 0.0002 0 0 0 0 0 0
Nucleus 0.0112 0.0002 0.0007 0.0003 0.0025 0.0002 0.0041 0.0002 0.0002 0.0055 0.0003 0.0002 0.0001 0 0.0014 0 0 0
Peroxisomes 0.0309 0.0024 0.0028 0.0202 0.0049 0.015 0.0077 0.0049 0.0108 0.0187 0.0282 0.0077 0.0023 0.0136 0.0037 0.0042 0.0005 0.0014
Punctate Nuclear 0.0049 0.0002 0.0008 0.0189 0.0036 0.0026 0.0013 0.0001 0.0003 0.0036 0.0099 0.003 0.0001 0.0001 0.0014 0.0001 0 0.0001
Vacuole 0.0085 0.0166 0.004 0.0133 0.0054 0.0024 0.0014 0.0056 0.0018 0.006 0.0047 0.0014 0.0013 0.0044 0.0024 0.0017 0.0006 0.0024
Vacuole Periphery 0.0099 0.0087 0.0048 0.0089 0.0105 0.0055 0.0024 0.0065 0.0023 0.0026 0.0039 0.0028 0.0031 0.0065 0.0035 0.0031 0.0016 0.0041

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 56.9161 47.8621 46.0161 57.3559 47.838 54.4417 60.5969 66.4606 63.3794 58.6625
Translational Efficiency 0.7619 0.7271 0.8195 0.6329 0.5748 0.8623 0.7256 0.5923 0.5946 0.6084

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency