Standard name | |
---|---|
Human Ortholog | |
Description | Type I transmembrane sorting receptor for multiple vacuolar hydrolases; cycles between the late-Golgi and prevacuolar endosome-like compartments |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 26 | 46 | 17 | 4 | 5 | 6 | 5 | 17 | 14 | 16 | 14 | 8 | 19 | 23 | 12 | – | – | – | 8 | 9 | 5 | 18 | 18 | 23 |
Bud | 1 | 2 | 5 | 4 | 0 | 10 | 18 | 33 | 12 | 23 | 9 | 17 | 4 | 4 | 2 | – | – | – | 2 | 0 | 0 | 2 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 3 | 1 | 0 | 1 | 0 | 0 | 0 | – | – | – | – | – | – | – | – | – |
Cell Periphery | 1 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 2 | 0 | 3 | 0 | 2 | 1 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 13 | 10 | 12 | 14 | 15 | 22 | 15 | 28 | 14 | 19 | 18 | 19 | 8 | 10 | 14 | – | – | – | 0 | 1 | 1 | 1 | 2 | 2 |
Endoplasmic Reticulum | 15 | 28 | 5 | 2 | 4 | 19 | 3 | 16 | 12 | 17 | 10 | 10 | 16 | 9 | 9 | – | – | – | 1 | 2 | 0 | 4 | 5 | 6 |
Endosome | 91 | 28 | 101 | 103 | 91 | 60 | 83 | 93 | 35 | 22 | 18 | 32 | 53 | 36 | 43 | – | – | – | 217 | 125 | 133 | 229 | 170 | 187 |
Golgi | 215 | 131 | 133 | 32 | 68 | 38 | 27 | 56 | 50 | 33 | 34 | 22 | 161 | 142 | 200 | – | – | – | 218 | 133 | 147 | 245 | 339 | 373 |
Mitochondria | 73 | 103 | 55 | 59 | 50 | 184 | 254 | 269 | 216 | 247 | 190 | 192 | 22 | 19 | 25 | – | – | – | 10 | 7 | 1 | 21 | 14 | 16 |
Nucleus | 1 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 1 | 0 | – | – | – | 0 | 1 | 1 | 0 | 0 | 0 |
Nuclear Periphery | 1 | 1 | 5 | 0 | 1 | 2 | 2 | 4 | 2 | 5 | 2 | 2 | 2 | 5 | 0 | – | – | – | 0 | 1 | 1 | 0 | 2 | 0 |
Nucleolus | 15 | 7 | 4 | 24 | 15 | 19 | 18 | 20 | 14 | 18 | 6 | 14 | 7 | 6 | 9 | – | – | – | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 6 | 5 | 1 | 1 | 7 | 7 | 3 | 5 | 3 | 1 | 1 | 2 | 0 | – | – | – | 2 | 1 | 7 | 11 | 6 | 7 |
SpindlePole | 5 | 0 | 5 | 9 | 4 | 3 | 9 | 7 | 6 | 12 | 1 | 5 | 4 | 2 | 3 | – | – | – | 1 | 1 | 1 | 2 | 0 | 1 |
Vac/Vac Membrane | 143 | 54 | 35 | 27 | 42 | 27 | 30 | 39 | 18 | 10 | 20 | 15 | 48 | 49 | 70 | – | – | – | 17 | 16 | 2 | 8 | 9 | 13 |
Unique Cell Count | 425 | 258 | 273 | 197 | 225 | 301 | 373 | 454 | 289 | 328 | 251 | 262 | 260 | 226 | 299 | 484 | 304 | 306 | 548 | 571 | 640 | |||
Labelled Cell Count | 600 | 410 | 383 | 283 | 296 | 392 | 473 | 592 | 400 | 430 | 326 | 342 | 345 | 310 | 388 | 484 | 304 | 306 | 548 | 571 | 640 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 12.7 | 13.1 | 11.3 | 10.9 | 10.3 | 9.2 | 8.7 | 8.9 | 8.5 | 7.9 | 8.2 | 7.8 | 12.5 | 12.6 | 12.9 | – | – | – | 11.5 | 13.3 | 14.3 |
Std Deviation (1e-4) | 2.0 | 2.3 | 1.9 | 2.0 | 1.8 | 1.5 | 1.6 | 1.6 | 1.8 | 1.6 | 1.6 | 1.5 | 2.0 | 2.3 | 2.3 | – | – | – | 2.4 | 2.8 | 3.4 |
Intensity Change (Log2) | – | – | – | -0.06 | -0.13 | -0.3 | -0.39 | -0.35 | -0.41 | -0.52 | -0.46 | -0.54 | 0.15 | 0.15 | 0.18 | – | – | – | 0.02 | 0.23 | 0.34 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | -2.2 | -2.2 | -2.6 | -3.4 | -1.5 | -0.7 | -0.7 | -0.3 | -1.7 | 0.5 | 1.6 | -1.2 | – | – | – |
Bud | 0 | 0 | 1.1 | 2.0 | 3.2 | 1.6 | 3.0 | 1.2 | 2.7 | 0 | 0 | 0 | – | – | – |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cell Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Cytoplasm | 1.3 | 1.1 | 1.5 | -0.2 | 1.0 | 0.3 | 0.8 | 1.4 | 1.4 | -0.8 | 0 | 0.2 | – | – | – |
Endoplasmic Reticulum | 0 | 0 | 2.7 | 0 | 1.3 | 1.6 | 2.2 | 1.5 | 1.4 | 2.6 | 1.4 | 0.9 | – | – | – |
Endosome | 3.3 | 0.8 | -4.5 | -4.1 | -4.9 | -6.9 | -9.2 | -8.1 | -6.6 | -4.2 | -5.2 | -6.2 | – | – | – |
Golgi | -7.3 | -4.2 | -9.4 | -12.1 | -10.8 | -7.9 | -10.6 | -8.6 | -10.3 | 3.1 | 3.2 | 4.4 | – | – | – |
Mitochondria | 2.4 | 0.6 | 9.9 | 12.1 | 10.3 | 12.9 | 13.5 | 12.7 | 12.3 | -3.8 | -3.7 | -4.1 | – | – | – |
Nucleus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Nucleolus | 4.8 | 3.0 | 3.0 | 2.3 | 2.1 | 2.3 | 2.6 | 0 | 2.5 | 1.0 | 0 | 1.2 | – | – | – |
Peroxisomes | 0 | 0 | 0 | -0.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – |
SpindlePole | 1.7 | 0 | 0 | 0.5 | 0 | 0.2 | 1.3 | 0 | 0 | 0 | 0 | 0 | – | – | – |
Vacuole | 0.3 | 1.8 | -1.5 | -2.0 | -1.8 | -2.7 | -4.5 | -1.8 | -2.8 | 1.8 | 2.6 | 3.3 | – | – | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | 7.8111 | 10.3482 | 9.4416 | 8.7717 | 8.6794 | 9.3808 | 10.03 | 11.9234 | 11.4161 | 11.0919 | 10.4038 | 11.437 | 10.3809 | 11.6528 | 11.4132 | 11.3294 | 11.3148 | 11.6717 |
Actin | 0.0242 | 0.0132 | 0.0272 | 0.0453 | 0.0097 | 0.0042 | 0.0288 | 0.0008 | 0.0139 | 0.0469 | 0.0452 | 0.0026 | 0.0211 | 0.0111 | 0.0517 | 0.0749 | 0.0002 | 0.003 |
Bud | 0.0006 | 0.0004 | 0.0014 | 0.0002 | 0.0004 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0.0007 | 0.0004 | 0 | 0.0001 | 0.0001 | 0.0003 | 0.0004 | 0 | 0 |
Bud Neck | 0.0007 | 0.0004 | 0.0011 | 0.0003 | 0.0003 | 0.0002 | 0.0003 | 0.0002 | 0.0003 | 0.0044 | 0.0007 | 0.0006 | 0.0003 | 0.0002 | 0.0006 | 0.0004 | 0.0001 | 0.0002 |
Bud Periphery | 0.0006 | 0.0004 | 0.0021 | 0.0002 | 0.0011 | 0.0001 | 0.0001 | 0.0002 | 0.0006 | 0.0019 | 0.0006 | 0 | 0.0001 | 0 | 0.0002 | 0.0006 | 0 | 0 |
Bud Site | 0.0024 | 0.0013 | 0.0068 | 0.0006 | 0.0007 | 0.0002 | 0.0005 | 0.0004 | 0.0004 | 0.0047 | 0.0009 | 0.0001 | 0.0003 | 0.0001 | 0.0029 | 0.0003 | 0 | 0.0001 |
Cell Periphery | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0.0001 | 0.0006 | 0.0001 | 0 | 0.0001 | 0 | 0.0001 | 0.0001 | 0 | 0 |
Cytoplasm | 0.0039 | 0.0066 | 0.0018 | 0.0017 | 0.001 | 0.0012 | 0.0063 | 0.0025 | 0.0054 | 0.0031 | 0.0041 | 0.0009 | 0.0019 | 0.0013 | 0.0164 | 0.001 | 0.0005 | 0.0009 |
Cytoplasmic Foci | 0.0691 | 0.0795 | 0.0575 | 0.0745 | 0.056 | 0.0427 | 0.0809 | 0.1033 | 0.083 | 0.0824 | 0.1381 | 0.0652 | 0.072 | 0.0589 | 0.0766 | 0.06 | 0.0764 | 0.0562 |
Eisosomes | 0.0001 | 0 | 0.0002 | 0.0001 | 0.0003 | 0 | 0.0002 | 0 | 0.0001 | 0.0005 | 0.0002 | 0.0001 | 0.0001 | 0 | 0.0002 | 0.0003 | 0 | 0 |
Endoplasmic Reticulum | 0.0028 | 0.0011 | 0.0021 | 0.0004 | 0.0014 | 0.0007 | 0.0014 | 0.0009 | 0.0007 | 0.0008 | 0.0019 | 0.0005 | 0.0008 | 0.0006 | 0.0017 | 0.0008 | 0.0001 | 0.0005 |
Endosome | 0.2861 | 0.3904 | 0.2802 | 0.4096 | 0.3501 | 0.2363 | 0.3062 | 0.3664 | 0.2831 | 0.2669 | 0.3026 | 0.2188 | 0.2551 | 0.4417 | 0.3101 | 0.3138 | 0.3597 | 0.3007 |
Golgi | 0.4453 | 0.4524 | 0.5687 | 0.3302 | 0.4497 | 0.6423 | 0.518 | 0.4669 | 0.5693 | 0.4946 | 0.3974 | 0.6535 | 0.5594 | 0.4488 | 0.5119 | 0.518 | 0.556 | 0.6078 |
Lipid Particles | 0.0342 | 0.0205 | 0.009 | 0.0157 | 0.0515 | 0.0351 | 0.0304 | 0.0108 | 0.015 | 0.0092 | 0.0489 | 0.0321 | 0.0756 | 0.0082 | 0.0082 | 0.0104 | 0.004 | 0.0204 |
Mitochondria | 0.046 | 0.0044 | 0.0204 | 0.0582 | 0.031 | 0.0088 | 0.0039 | 0.0291 | 0.0113 | 0.0298 | 0.0061 | 0.0079 | 0.0058 | 0.0041 | 0.0041 | 0.0097 | 0.0002 | 0.0021 |
None | 0.0012 | 0.0001 | 0.0003 | 0.0006 | 0.0009 | 0.0006 | 0.0026 | 0.0001 | 0.0002 | 0.0051 | 0.0023 | 0.0016 | 0.0001 | 0 | 0.0006 | 0.0001 | 0 | 0 |
Nuclear Periphery | 0.0158 | 0.001 | 0.0079 | 0.0005 | 0.0164 | 0.0016 | 0.0032 | 0.0003 | 0.0007 | 0.0013 | 0.0031 | 0.0007 | 0.0002 | 0.0002 | 0.0021 | 0.0002 | 0 | 0.0002 |
Nucleolus | 0.0012 | 0.0001 | 0.0002 | 0.0003 | 0.0023 | 0.0001 | 0.0001 | 0.0002 | 0.0001 | 0.0105 | 0.0004 | 0.0002 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.0112 | 0.0002 | 0.0007 | 0.0003 | 0.0025 | 0.0002 | 0.0041 | 0.0002 | 0.0002 | 0.0055 | 0.0003 | 0.0002 | 0.0001 | 0 | 0.0014 | 0 | 0 | 0 |
Peroxisomes | 0.0309 | 0.0024 | 0.0028 | 0.0202 | 0.0049 | 0.015 | 0.0077 | 0.0049 | 0.0108 | 0.0187 | 0.0282 | 0.0077 | 0.0023 | 0.0136 | 0.0037 | 0.0042 | 0.0005 | 0.0014 |
Punctate Nuclear | 0.0049 | 0.0002 | 0.0008 | 0.0189 | 0.0036 | 0.0026 | 0.0013 | 0.0001 | 0.0003 | 0.0036 | 0.0099 | 0.003 | 0.0001 | 0.0001 | 0.0014 | 0.0001 | 0 | 0.0001 |
Vacuole | 0.0085 | 0.0166 | 0.004 | 0.0133 | 0.0054 | 0.0024 | 0.0014 | 0.0056 | 0.0018 | 0.006 | 0.0047 | 0.0014 | 0.0013 | 0.0044 | 0.0024 | 0.0017 | 0.0006 | 0.0024 |
Vacuole Periphery | 0.0099 | 0.0087 | 0.0048 | 0.0089 | 0.0105 | 0.0055 | 0.0024 | 0.0065 | 0.0023 | 0.0026 | 0.0039 | 0.0028 | 0.0031 | 0.0065 | 0.0035 | 0.0031 | 0.0016 | 0.0041 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 56.9161 | 47.8621 | 46.0161 | 57.3559 | 47.838 | 54.4417 | 60.5969 | 66.4606 | 63.3794 | 58.6625 |
Translational Efficiency | 0.7619 | 0.7271 | 0.8195 | 0.6329 | 0.5748 | 0.8623 | 0.7256 | 0.5923 | 0.5946 | 0.6084 |
Dataset | Hit |
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Protein Concentration | ✘ |
Protein Localization | ✘ |
Gene Expression | ✔ |
Translational Efficiency | ✘ |
Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
---|---|---|---|---|
37℃ | ||||
RT |
Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
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Gene | Images |
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Gene | Images |
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