Standard name
Description Glycerol 3-phosphate/dihydroxyacetone phosphate sn-1 acyltransferase; dual substrate-specific acyltransferase of the glycerolipid biosynthesis pathway; prefers 16-carbon fatty acids; similar to Gpt2p; gene is constitutively transcribed

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0.07 0 0.09 0.1 0 0 0 0.05 0.06 0 0.06 0.07 0.11 0.16 0.17 0.15 0.31 0 0 0 0 0 0
Cytoplasm 0.14 0.14 0.16 0.16 0.18 0.25 0.25 0.25 0.25 0.26 0.31 0.22 0.24 0.28 0.31 0.5 0.64 0.52 0 0 0 0 0 0
Endoplasmic Reticulum 0.82 0.84 0.66 0.63 0.25 0.49 0.31 0.31 0.23 0.21 0.27 0.23 0.72 0.67 0.44 0 0 0 0.89 0.86 0.9 0.81 0.79 0.77
Endosome 0 0 0 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0.06 0.16 0.09 0.28 0.36 0.25 0.43 0.46 0.45 0.5 0 0 0 0.11 0.11 0.07 0 0 0 0.05 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0.07 0.05 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0.18 0 0.21 0.12 0.5 0.15 0.22 0.28 0.25 0.22 0.11 0.16 0 0.07 0.24 0.2 0.12 0.14 0 0 0 0 0.06 0.09
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 10 10 3 8 0 7 2 8 2 4 2 1 3 3 1 11 1 0 7 7 3 4 6 4
Bud 0 0 0 0 1 3 1 11 14 9 8 6 1 2 0 1 0 0 2 1 0 0 3 3
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 2 1 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cell Periphery 10 23 9 15 21 12 15 21 15 23 7 21 5 21 25 41 17 29 1 2 2 1 4 2
Cytoplasm 56 48 37 25 39 69 83 118 70 98 65 73 17 53 48 118 72 49 12 13 2 1 9 9
Endoplasmic Reticulum 331 292 154 102 55 136 105 147 63 79 58 75 52 127 68 4 1 0 387 325 243 124 229 203
Endosome 1 0 1 1 11 2 5 9 4 3 2 3 0 0 0 3 1 1 2 2 0 0 1 5
Golgi 4 1 2 1 3 0 1 0 0 0 0 0 0 0 0 2 0 0 2 3 1 0 1 2
Mitochondria 4 6 14 26 20 79 120 118 118 175 95 164 0 0 0 27 12 7 5 2 5 7 10 4
Nucleus 0 1 0 2 2 0 4 8 3 3 2 5 0 4 0 7 4 2 0 1 0 0 0 0
Nuclear Periphery 5 7 8 7 3 2 25 22 10 12 11 15 0 1 0 4 2 1 2 0 3 2 0 1
Nucleolus 0 0 0 0 0 0 1 2 0 2 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 1 0 1 0 1 0 0 0 0 0 0 0 0 0 0 2 0 0 0 0 0 0 0 1
Vac/Vac Membrane 72 15 48 19 110 43 74 130 68 83 23 52 1 13 36 48 14 13 6 11 1 4 17 23
Unique Cell Count 403 346 232 161 221 280 334 471 277 379 211 328 72 190 153 236 112 95 438 378 271 153 290 264
Labelled Cell Count 494 403 278 206 266 353 436 594 367 492 273 415 79 224 178 268 125 102 438 378 271 153 290 264


Endoplasmic Reticulum

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 10.0 9.9 9.5 9.3 8.8 7.5 7.2 7.3 6.4 6.0 6.1 6.0 8.9 9.3 9.8 6.8 7.3 9.4 9.7 10.5 11.1
Std Deviation (1e-4) 1.3 1.4 1.6 1.8 1.3 1.6 1.3 1.4 1.1 1.2 1.2 1.1 1.1 1.3 1.1 1.1 1.2 1.5 1.8 1.9 1.6
Intensity Change (Log2) -0.03 -0.11 -0.33 -0.39 -0.38 -0.57 -0.66 -0.63 -0.67 -0.09 -0.03 0.05 -0.48 -0.37 -0.02 0.03 0.15 0.23


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 2.1 0 0
Bud 0 0 0 0 0 3.5 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 2.2 2.4 0.2 0.4 0.4 0.8 1.2 -0.3 1.3 0 2.9 4.2 4.7 3.7 6.8
Cytoplasm -0.1 0.5 2.4 2.5 2.7 2.6 2.9 3.7 1.9 1.5 3.0 3.6 7.8 9.0 6.6
Endoplasmic Reticulum -0.6 -8.9 -4.0 -8.2 -8.9 -9.9 -11.2 -8.2 -10.3 0.9 0.1 -4.3 -14.8 -11.4 -10.9
Endosome 0 3.0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 3.3 1.2 6.5 8.2 6.1 9.4 10.4 9.5 11.0 0 -3.4 -3.1 2.1 1.5 0.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0.5 -1.4 0 2.0 0.8 0.1 -0.2 0.9 0.7 0 0 0 -1.2 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole -2.3 6.5 -1.6 0.4 2.0 1.0 0.4 -2.8 -1.5 -3.9 -4.0 0.7 -0.1 -1.9 -1.5


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Sct1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available