ORF
Description Putative protein of unknown function; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.94 0.86 0.99 0.95 0.94 0.89 0.9 0.88 0.72 0.73 0.59 0.59 0.94 0.99 0.99 0.99 0.93 0.84 0.84 0.69 0.91 0.89 0.86
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.09 0 0 0.06 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0.08 0.18 0 0.07 0.06 0.16 0.11 0.15 0.49 0.45 0.66 0.63 0 0 0 0 0 0.05 0.07 0.15 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 1
Bud 2 1 1 1 0 0 0 1 9 5 3 1 0 0 0 0 1 0 0 0 0 2 4
Bud Neck 0 0 0 0 0 0 1 0 0 1 0 0 0 0 0 0 0 1 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 1 1 3 3 4 1 1 1 3 0 0 1 2 2 5 0 0 0 0 0 0
Cytoplasm 103 65 88 125 117 128 193 276 155 179 82 101 17 200 262 75 111 102 90 82 99 160 210
Endoplasmic Reticulum 4 3 1 0 0 0 1 0 2 0 0 2 0 2 1 2 11 4 1 7 0 1 3
Endosome 0 0 0 0 0 2 5 2 0 1 1 0 0 1 1 0 0 1 1 1 0 0 4
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0
Mitochondria 9 14 0 9 7 23 24 47 105 109 92 109 0 0 0 3 3 5 8 17 3 3 6
Nucleus 0 0 1 3 4 2 2 4 2 2 3 4 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 1 1 0 0 0 0 0 0 1 1 0 0 1 0 1 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 1 2 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0
SpindlePole 0 0 0 0 0 0 0 2 1 0 1 0 0 0 0 0 0 0 0 0 0 1 2
Vac/Vac Membrane 0 0 0 2 2 5 6 10 3 2 1 2 0 0 1 0 3 3 2 3 1 2 4
Unique Cell Count 110 76 89 132 125 144 214 312 216 244 140 172 18 203 265 76 119 122 107 119 109 180 245
Labelled Cell Count 119 85 92 142 135 163 236 344 280 301 188 220 18 204 268 82 135 122 107 119 109 180 245


Ambiguous

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 5.0 3.8 5.6 5.3 6.3 5.2 5.2 5.4 4.2 4.5 4.2 4.2 4.8 6.2 5.6 4.2 5.2 5.8 6.3 6.7 6.6
Std Deviation (1e-4) 0.7 0.6 1.2 1.3 1.8 1.6 1.4 1.9 1.1 1.3 1.3 1.0 0.5 2.2 1.5 0.8 0.8 1.3 1.5 1.9 1.9
Intensity Change (Log2) -0.06 0.18 -0.09 -0.1 -0.03 -0.42 -0.31 -0.4 -0.42 -0.21 0.17 0.01 -0.42 -0.09 0.05 0.18 0.27 0.25


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole