| Standard name | |
|---|---|
| Description | Histone H3; core histone protein required for chromatin assembly, part of heterochromatin-mediated telomeric and HM silencing; one of two identical histone H3 proteins (see HHT2); regulated by acetylation, methylation, and phosphorylation; H3K14 acetylation plays an important role in the unfolding of strongly positioned nucleosomes during repair of UV damage |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 0 | 1 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 3 | 1 | 5 | 0 | 2 | 0 |
| Bud | 0 | 1 | 3 | 5 | 1 | 5 | – | 23 | 6 | 16 | 16 | 11 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 3 | 2 | 4 | 9 |
| Bud Neck | 2 | 0 | 2 | 0 | 0 | 0 | – | 8 | 0 | 1 | 3 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 1 | 2 |
| Bud Site | 0 | 0 | 0 | 0 | 0 | 1 | – | 1 | 4 | 2 | 2 | 7 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
| Cell Periphery | 2 | 0 | 2 | 3 | 1 | 0 | – | 6 | 0 | 2 | 1 | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 3 | 1 | 0 | 0 |
| Cytoplasm | 354 | 187 | 185 | 228 | 281 | 295 | – | 440 | 227 | 326 | 157 | 218 | 211 | 278 | 348 | 20 | 36 | 38 | 305 | 160 | 126 | 130 | 205 | 207 |
| Endoplasmic Reticulum | 0 | 1 | 1 | 0 | 1 | 1 | – | 2 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 2 | 1 | 3 | 0 | 1 | 2 | 0 | 1 | 0 |
| Endosome | 0 | 1 | 0 | 0 | 7 | 1 | – | 8 | 0 | 1 | 0 | 3 | 0 | 1 | 1 | 0 | 2 | 2 | 1 | 1 | 4 | 1 | 0 | 4 |
| Golgi | 0 | 0 | 0 | 0 | 1 | 0 | – | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 0 | 1 | 1 | 1 | 1 |
| Mitochondria | 8 | 3 | 25 | 58 | 35 | 141 | – | 247 | 198 | 251 | 189 | 274 | 1 | 1 | 0 | 1 | 0 | 1 | 17 | 17 | 26 | 6 | 19 | 14 |
| Nucleus | 0 | 0 | 0 | 1 | 2 | 1 | – | 4 | 1 | 2 | 2 | 3 | 0 | 1 | 0 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 2 |
| Nuclear Periphery | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 1 |
| Nucleolus | 0 | 0 | 0 | 0 | 1 | 0 | – | 3 | 1 | 1 | 3 | 1 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
| Peroxisomes | 1 | 0 | 0 | 0 | 2 | 0 | – | 2 | 0 | 1 | 0 | 2 | 0 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| SpindlePole | 0 | 0 | 2 | 0 | 0 | 4 | – | 20 | 5 | 11 | 17 | 18 | 0 | 0 | 1 | 0 | 0 | 0 | 5 | 2 | 4 | 1 | 0 | 2 |
| Vac/Vac Membrane | 0 | 1 | 1 | 1 | 1 | 6 | – | 7 | 6 | 7 | 4 | 3 | 0 | 1 | 0 | 0 | 0 | 4 | 2 | 3 | 4 | 4 | 6 | 6 |
| Unique Cell Count | 360 | 193 | 199 | 239 | 304 | 365 | 628 | 352 | 480 | 294 | 419 | 212 | 282 | 350 | 20 | 38 | 43 | 348 | 193 | 197 | 156 | 250 | 263 | |
| Labelled Cell Count | 367 | 195 | 221 | 297 | 333 | 455 | 771 | 450 | 622 | 395 | 543 | 212 | 284 | 352 | 25 | 39 | 50 | 348 | 193 | 197 | 156 | 250 | 263 | |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.4 | 4.0 | 3.2 | 3.2 | 4.2 | 3.1 | – | 3.1 | 2.6 | 3.0 | 2.8 | 2.6 | 4.5 | 4.5 | 4.6 | 5.0 | 5.5 | 5.7 | 4.3 | 4.6 | 4.8 |
| Std Deviation (1e-4) | 0.4 | 0.6 | 1.6 | 1.9 | 2.7 | 2.0 | – | 1.9 | 1.5 | 1.8 | 1.9 | 1.2 | 2.3 | 2.2 | 2.3 | 0.6 | 1.9 | 1.9 | 2.2 | 2.3 | 2.3 |
| Intensity Change (Log2) | – | – | – | 0.01 | 0.41 | -0.05 | – | -0.02 | -0.28 | -0.08 | -0.17 | -0.3 | 0.51 | 0.49 | 0.52 | 0.66 | 0.79 | 0.84 | 0.43 | 0.52 | 0.58 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
