Standard name
Human Ortholog
Description Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.09 0.06 0.07 0 0 0 0 0 0 0 0 0.14 0.15 0.12 0.1 0.11 0.08 0.05 0 0.08 0.12 0.11 0.13
Bud 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0.2 0.08 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0.15 0.08 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.22 0.35 0.24 0.58 0.39 0.51 0.49 0.51 0.62 0.6 0.67 0.67 0.53 0.52 0.59 0.34 0.19 0.21 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.15 0.08 0.07 0.05 0.05 0 0 0 0 0 0 0.13 0.11 0.14 0 0 0.06 0.06 0 0.06 0.07 0.16 0.2
Endosome 0.13 0.06 0.19 0.11 0.16 0.1 0.11 0.07 0.06 0.05 0 0.06 0.08 0.09 0.06 0.17 0.19 0.21 0.12 0.15 0.12 0 0 0
Golgi 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0.07 0.11 0.16 0.1 0.09 0.14 0 0 0
Mitochondria 0 0.1 0.11 0 0 0 0 0 0.07 0.11 0.14 0.1 0 0 0 0.12 0.13 0.09 0 0 0 0.05 0.05 0.05
Nucleus 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.08 0.1 0 0 0 0 0 0 0
Vac/Vac Membrane 0.55 0.23 0.33 0.17 0.43 0.39 0.43 0.4 0.34 0.38 0.21 0.21 0.05 0.05 0 0.3 0.34 0.35 0.55 0.49 0.45 0.34 0.44 0.45
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 29 11 14 15 6 11 5 2 4 4 5 4 23 25 23 23 16 13 28 7 25 32 47 55
Bud 5 6 3 4 8 4 12 18 12 9 11 23 6 6 2 9 6 2 22 10 8 56 35 24
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 28 11 17 42 36 20
Bud Site 7 2 9 6 6 7 2 0 1 1 2 7 0 0 1 3 5 1 – – – – – –
Cell Periphery 1 2 3 7 2 0 3 2 3 1 1 1 2 2 7 2 0 1 0 0 0 4 5 5
Cytoplasm 106 44 55 125 127 167 219 306 208 228 174 239 89 89 110 81 26 33 2 3 1 4 3 5
Endoplasmic Reticulum 30 19 19 15 15 16 6 7 9 1 4 3 22 19 27 7 5 9 40 7 17 19 68 90
Endosome 65 8 43 24 53 33 49 43 19 20 8 20 14 15 11 41 27 34 74 26 37 3 6 7
Golgi 25 7 12 2 1 1 0 0 0 0 1 0 9 10 5 17 16 26 64 15 42 1 10 7
Mitochondria 19 13 24 6 5 13 17 23 22 41 36 37 5 5 1 29 18 15 9 4 6 15 20 21
Nucleus 11 1 1 1 3 9 23 29 10 7 3 4 2 2 2 9 1 2 0 0 0 1 0 0
Nuclear Periphery 0 1 1 0 0 0 0 1 1 4 1 0 0 0 0 1 0 0 2 0 1 1 2 3
Nucleolus 3 3 2 2 5 5 5 19 1 2 1 5 0 0 0 0 1 2 1 0 0 0 0 0
Peroxisomes 11 3 15 2 9 4 3 2 2 0 1 2 0 2 0 3 6 3 3 1 4 0 0 0
SpindlePole 10 1 11 4 10 2 5 4 2 2 2 3 9 8 8 19 14 4 1 0 0 0 0 0
Vac/Vac Membrane 264 29 76 36 138 127 190 239 114 144 53 75 8 8 8 72 47 55 346 85 136 93 190 197
Unique Cell Count 482 127 227 217 324 326 447 597 336 380 258 357 169 170 188 240 140 159 628 175 304 279 432 444
Labelled Cell Count 586 150 288 249 388 399 539 695 408 464 303 423 189 191 206 316 189 200 628 175 304 279 432 444


Late Golgi

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 8.5 8.3 8.0 8.5 7.5 7.2 7.3 7.1 6.5 6.6 6.3 7.4 7.4 7.1 6.7 7.6 9.3 9.9 10.8 11.7
Std Deviation (1e-4) 1.4 1.5 1.7 1.9 1.8 1.6 1.5 1.8 1.6 1.4 1.6 1.3 1.1 1.1 1.1 1.1 1.6 2.2 2.0 2.4 2.5
Intensity Change (Log2) – – – -0.06 0.03 -0.14 -0.2 -0.19 -0.24 -0.36 -0.34 -0.4 -0.17 -0.17 -0.22 -0.31 -0.13 0.16 0.25 0.37 0.49


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 -2.7 -1.6 -3.7 0 -3.3 -3.6 -2.4 -3.4 2.5 2.8 2.2 1.4 1.8 0.8
Bud 0 0 0 1.1 1.4 1.6 0 1.9 2.9 0 0 0 1.7 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -0.7 -1.5 -1.3 0 0 0 0 -2.4 -1.4 0 0 0 -1.9 -0.2 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 7.2 3.7 6.4 6.2 7.0 8.8 8.5 9.5 10.1 5.8 5.8 7.1 2.3 -1.3 -0.8
Endoplasmic Reticulum -0.6 -1.8 -1.6 -4.6 -5.3 -3.0 -5.4 -3.5 -4.7 1.5 0.9 1.9 -2.6 -1.8 -1.0
Endosome -2.3 -0.8 -3.0 -2.9 -4.9 -4.9 -5.3 -5.7 -5.1 -3.0 -2.8 -3.9 -0.5 0.1 0.6
Golgi -2.6 -3.8 -3.8 0 0 0 0 -3.3 0 0 0.3 -1.3 0.8 2.2 3.6
Mitochondria -3.3 -4.7 -3.0 -3.5 -3.7 -1.7 0.1 1.1 -0.1 -2.9 -2.9 -4.3 0.5 0.7 -0.4
Nucleus 0 0 0 3.1 3.0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1.9 0 0 0 0 0 0 0 0 0 0
Peroxisomes -3.1 -2.2 -3.4 -4.5 0 -4.1 -5.1 -3.8 -4.2 -3.4 -2.6 -3.6 -3.0 -0.9 -2.2
SpindlePole -1.8 -1.1 -3.2 -3.0 0 -3.3 0 -2.8 -3.1 0.2 -0.1 -0.3 1.4 1.9 -1.2
Vacuole -4.1 2.2 1.3 2.3 1.7 0.1 1.1 -3.2 -3.4 -6.9 -6.9 -7.4 -0.8 0 0.2

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 3.4283 6.0396 5.146 4.7011 4.0667 4.8833 5.4419 6.5388 6.8275 5.6053 6.0519 6.5886 5.99 6.9577 6.5664 6.7892 7.1822 6.4801
Actin 0.0828 0.0245 0.068 0.0057 0.0161 0.0512 0.0929 0.0506 0.0507 0.0014 0.0018 0.0768 0.1566 0.0056 0.0924 0.0064 0.0007 0.0329
Bud 0.0045 0.0041 0.0036 0.0017 0.0007 0.0016 0.0037 0.0106 0.0031 0.0006 0.002 0.0019 0.0015 0.0023 0.0039 0.0003 0.0003 0.0013
Bud Neck 0.0284 0.0205 0.073 0.0106 0.0254 0.4361 0.0208 0.0291 0.0684 0.0016 0.0312 0.3534 0.0026 0.043 0.0607 0.0011 0.0007 0.3544
Bud Periphery 0.0072 0.0043 0.0064 0.0199 0.0008 0.0027 0.0054 0.0123 0.0037 0.0008 0.0017 0.0037 0.0012 0.0031 0.0035 0.0003 0.0002 0.0027
Bud Site 0.1169 0.2757 0.1023 0.0012 0.0027 0.0042 0.0957 0.3963 0.092 0.0011 0.0073 0.0041 0.0032 0.2127 0.0807 0.0004 0.0006 0.0044
Cell Periphery 0.0115 0.0048 0.0044 0.003 0.0005 0.0011 0.0134 0.0059 0.004 0.0002 0.0004 0.0022 0.0135 0.0122 0.0012 0.0001 0 0.0033
Cytoplasm 0.0967 0.0401 0.0167 0.0049 0.0083 0.0555 0.0816 0.0527 0.0347 0.0093 0.02 0.0553 0.0497 0.1669 0.0097 0.0021 0.0107 0.0607
Cytoplasmic Foci 0.1072 0.1073 0.103 0.1025 0.1127 0.0865 0.1363 0.0654 0.1173 0.138 0.1684 0.0886 0.1114 0.1277 0.1563 0.1015 0.1327 0.0984
Eisosomes 0.0005 0.0001 0.0003 0 0.0001 0.0005 0.0002 0.0001 0.0002 0 0 0.0002 0.0002 0.0001 0.0002 0.0001 0 0.0002
Endoplasmic Reticulum 0.0109 0.0042 0.0027 0.0012 0.0056 0.0058 0.0069 0.0018 0.0018 0.0015 0.0017 0.0066 0.0295 0.0086 0.0012 0.0013 0.0015 0.0139
Endosome 0.2163 0.2164 0.239 0.4194 0.3799 0.1525 0.2743 0.1446 0.2808 0.4659 0.4368 0.1694 0.2111 0.1072 0.2361 0.3249 0.4571 0.1387
Golgi 0.1167 0.1488 0.2516 0.3372 0.3188 0.1384 0.1117 0.1315 0.2162 0.2863 0.259 0.1375 0.1207 0.1319 0.2848 0.4667 0.3632 0.1807
Lipid Particles 0.0375 0.0355 0.0198 0.0258 0.0217 0.016 0.0647 0.0074 0.0259 0.0379 0.0249 0.0158 0.1802 0.0263 0.0147 0.0195 0.0096 0.0389
Mitochondria 0.0259 0.0058 0.0339 0.0075 0.0126 0.009 0.0043 0.0092 0.0204 0.0147 0.0095 0.0208 0.0021 0.0202 0.0112 0.058 0.0041 0.0123
None 0.0075 0.0107 0.0009 0.0001 0.0021 0.0076 0.0063 0.0017 0.0034 0.0001 0.0007 0.0133 0.0157 0.0135 0.0003 0.0001 0.0001 0.0006
Nuclear Periphery 0.0068 0.0027 0.0017 0.0006 0.0092 0.0027 0.002 0.0006 0.0013 0.0004 0.0005 0.0013 0.0416 0.0018 0.0002 0.0002 0.0004 0.0006
Nucleolus 0.0018 0.0014 0.0009 0.0003 0.0005 0.0007 0.0005 0.0011 0.0019 0.0001 0.0003 0.0005 0.001 0.0014 0.0003 0.0001 0 0.0004
Nucleus 0.0075 0.0019 0.001 0.0004 0.0027 0.0011 0.0009 0.0014 0.0017 0.0002 0.0004 0.0011 0.0137 0.0015 0.0004 0.0001 0.0001 0.0006
Peroxisomes 0.04 0.0187 0.0241 0.0032 0.0247 0.0104 0.019 0.012 0.034 0.0173 0.0177 0.0224 0.0037 0.0131 0.0314 0.0105 0.0044 0.0266
Punctate Nuclear 0.0131 0.0007 0.0014 0.0008 0.0116 0.0019 0.0015 0.0018 0.0021 0.0003 0.0005 0.0042 0.0145 0.0017 0.0007 0.0002 0.0001 0.0005
Vacuole 0.055 0.0661 0.0374 0.0495 0.0383 0.0117 0.0542 0.0589 0.0325 0.0167 0.0115 0.0181 0.0234 0.0969 0.0082 0.0038 0.0092 0.0256
Vacuole Periphery 0.0053 0.0057 0.0078 0.0044 0.005 0.0028 0.0036 0.0051 0.0039 0.0054 0.0036 0.0028 0.0029 0.0023 0.0018 0.0024 0.0041 0.0023

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 68.2925 35.2479 31.805 41.2763 66.6637 61.1612 40.5573 29.5383 36.3119 64.5321
Translational Efficiency 0.6925 0.7054 0.6293 0.6086 0.5361 0.7201 0.5299 0.5722 0.5176 0.5187

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✔
Gene Expression ✔
Translational Efficiency ✘
Standard name
Human Ortholog
Description Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention
Localization
Cell Percentages —
Cell Cycle Regulation No
Subcompartmental Group N/A

Chs3

Chs3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Chs3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available