Standard name
Description Chitin synthase III; catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan; contains overlapping di-leucine and di-acidic signals that mediate, respectively, intracellular trafficking by AP-1 and trafficking to plasma membrane by exomer complex; requires AP-3 complex for its intracellular retention

Micrographs

Sub-cellular Localization

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.06 0.09 0.06 0.07 0 0 0 0 0 0 0 0 0.14 0.15 0.12 0.10 0.11 0.08 0.05 0 0.08 0.12 0.11 0.13
Bud 0 0.05 0 0 0 0 0 0 0 0 0 0.06 0 0 0 0 0 0 0 0.06 0 0.20 0.08 0.06
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0.06 0.06 0.15 0.08 0.05
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.22 0.35 0.24 0.58 0.39 0.51 0.49 0.51 0.62 0.60 0.67 0.67 0.53 0.52 0.59 0.34 0.19 0.21 0 0 0 0 0 0
Endoplasmic Reticulum 0.06 0.15 0.08 0.07 0.05 0.05 0 0 0 0 0 0 0.13 0.11 0.14 0 0 0.06 0.06 0 0.06 0.07 0.16 0.20
Endosome 0.13 0.06 0.19 0.11 0.16 0.10 0.11 0.07 0.06 0.05 0 0.06 0.08 0.09 0.06 0.17 0.19 0.21 0.12 0.15 0.12 0 0 0
Golgi 0.05 0.06 0.05 0 0 0 0 0 0 0 0 0 0.05 0.06 0 0.07 0.11 0.16 0.10 0.09 0.14 0 0 0
Mitochondria 0 0.10 0.11 0 0 0 0 0 0.07 0.11 0.14 0.10 0 0 0 0.12 0.13 0.09 0 0 0 0.05 0.05 0.05
Nucleus 0 0 0 0 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0.07 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.05 0 0 0 0 0 0 0 0 0 0.05 0.05 0 0.08 0.10 0 0 0 0 0 0 0
Vac/Vac Membrane 0.55 0.23 0.33 0.17 0.43 0.39 0.43 0.40 0.34 0.38 0.21 0.21 0.05 0.05 0 0.30 0.34 0.35 0.55 0.49 0.45 0.34 0.44 0.45
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 29 11 14 15 6 11 5 2 4 4 5 4 23 25 23 23 16 13 28 7 25 32 47 55
Bud 5 6 3 4 8 4 12 18 12 9 11 23 6 6 2 9 6 2 22 10 8 56 35 24
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 1 0 28 11 17 42 36 20
Bud Site 7 2 9 6 6 7 2 0 1 1 2 7 0 0 1 3 5 1
Cell Periphery 1 2 3 7 2 0 3 2 3 1 1 1 2 2 7 2 0 1 0 0 0 4 5 5
Cytoplasm 106 44 55 125 127 167 219 306 208 228 174 239 89 89 110 81 26 33 2 3 1 4 3 5
Endoplasmic Reticulum 30 19 19 15 15 16 6 7 9 1 4 3 22 19 27 7 5 9 40 7 17 19 68 90
Endosome 65 8 43 24 53 33 49 43 19 20 8 20 14 15 11 41 27 34 74 26 37 3 6 7
Golgi 25 7 12 2 1 1 0 0 0 0 1 0 9 10 5 17 16 26 64 15 42 1 10 7
Mitochondria 19 13 24 6 5 13 17 23 22 41 36 37 5 5 1 29 18 15 9 4 6 15 20 21
Nucleus 11 1 1 1 3 9 23 29 10 7 3 4 2 2 2 9 1 2 0 0 0 1 0 0
Nuclear Periphery 0 1 1 0 0 0 0 1 1 4 1 0 0 0 0 1 0 0 2 0 1 1 2 3
Nucleolus 3 3 2 2 5 5 5 19 1 2 1 5 0 0 0 0 1 2 1 0 0 0 0 0
Peroxisomes 11 3 15 2 9 4 3 2 2 0 1 2 0 2 0 3 6 3 3 1 4 0 0 0
SpindlePole 10 1 11 4 10 2 5 4 2 2 2 3 9 8 8 19 14 4 1 0 0 0 0 0
Vac/Vac Membrane 264 29 76 36 138 127 190 239 114 144 53 75 8 8 8 72 47 55 346 85 136 93 190 197
Unique Cell Count 482 127 227 217 324 326 447 597 336 380 258 357 169 170 188 240 140 159 628 175 304 279 432 444
Labelled Cell Count 586 150 288 249 388 399 539 695 408 464 303 423 189 191 206 316 189 200 628 175 304 279 432 444

Yeast GFP Assignment

(Huh et al. Nature 2003 425:686 )

Late Golgi

Protein Abundance

Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 8.9 8.5 8.3 8.0 8.5 7.5 7.2 7.3 7.1 6.5 6.6 6.3 7.4 7.4 7.1 6.7 7.6 9.3 9.9 10.8 11.7
Std Deviation (1e-4) 1.4 1.5 1.7 1.9 1.8 1.6 1.5 1.8 1.6 1.4 1.6 1.3 1.1 1.1 1.1 1.1 1.6 2.2 2.0 2.4 2.5
Intensity Change (Log2) -0.06 0.03 -0.14 -0.2 -0.19 -0.24 -0.36 -0.34 -0.4 -0.17 -0.17 -0.22 -0.31 -0.13 0.16 0.25 0.37 0.49

Localization Change

Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 -2.7 -1.6 -3.7 0 -3.3 -3.6 -2.4 -3.4 2.5 2.8 2.2 1.4 1.8 0.8
Bud 0 0 0 1.1 1.4 1.6 0 1.9 2.9 0 0 0 1.7 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site -0.7 -1.5 -1.3 0 0 0 0 -2.4 -1.4 0 0 0 -1.9 -0.2 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 2.3 2.0 0.7 0.4 1.8 1.7 1.9 2.4 2.5 2.3 2.3 2.1 2.7 0.8 1.1
Endoplasmic Reticulum -0.6 -1.8 -1.6 -4.6 -5.3 -3.0 -5.4 -3.5 -4.7 1.5 0.9 1.9 -2.6 -1.8 -1.0
Endosome -2.3 -0.8 -3.0 -2.9 -4.9 -4.9 -5.3 -5.7 -5.1 -3.0 -2.8 -3.9 -0.5 0.1 0.6
Golgi -2.6 -3.8 -3.8 0 0 0 0 -3.3 0 0 0.3 -1.3 0.8 2.2 3.6
Mitochondria -3.3 -4.7 -3.0 -3.5 -3.7 -1.7 0.1 1.1 -0.1 -2.9 -2.9 -4.3 0.5 0.7 -0.4
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 1.9 0 0 0 0 0 0 0 0 0 0
Peroxisomes -3.1 -2.2 -3.4 -4.5 0 -4.1 -5.1 -3.8 -4.2 -3.4 -2.6 -3.6 -3.0 -0.9 -2.2
SpindlePole -1.8 -1.1 -3.2 -3.0 0 -3.3 0 -2.8 -3.1 0.2 -0.1 -0.3 1.4 1.9 -1.2
Vacuole -1.2 2.1 1.0 2.2 3.2 1.6 1.2 -1.4 -1.9 -2.6 -2.6 -2.9 2.9 2.0 0.8