Standard name
Human Ortholog
Description P-loop ATPase with similarity to human OLA1 and bacterial YchF; identified as specifically interacting with the proteasome; null mutant displays increased translation rate and increased readthrough of premature stop codons; protein abundance increases in response to hydrogen peroxide and to DNA replication stress

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0.06 0.08 0.05 0.08 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0.06 0.06 0.12 0.09 0.08 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.99 0.99 0.98 0.97 0.91 0.91 0.77 0.72 0.66 0.66 0.66 0.65 0.99 0.99 0.99 0.99 0.99 0.98 0.98 0.96 0.95 0.96 0.91 0.9
Endoplasmic Reticulum 0 0 0 0 0.07 0.06 0.15 0.19 0.22 0.16 0.18 0.17 0 0 0 0.06 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0.05 0 0 0.1 0.13 0.11 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 3 4 18 12 21 29 11 24 0 0 0 0 0 0 0 0 0 0 2 0
Bud 0 1 1 0 0 0 2 1 1 2 0 4 0 0 2 0 0 0 0 0 0 0 3 6
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 1 3 6 5 12 30 23 42 34 17 33 6 7 8 8 4 6 0 0 2 0 0 0
Cytoplasm 181 273 512 205 297 357 356 282 229 245 144 192 294 255 284 580 480 388 180 254 503 163 179 166
Endoplasmic Reticulum 2 2 9 5 24 22 69 76 77 59 40 50 3 2 2 35 17 16 0 0 17 2 2 5
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 3 5 5 4 2 5 0 0 0 0 2 1 0 0 0 0 0 0
Mitochondria 0 0 0 0 1 10 22 12 11 37 29 31 0 0 0 0 1 0 0 0 1 0 0 0
Nucleus 0 0 0 0 1 1 0 2 0 0 0 0 0 0 0 0 1 0 0 2 0 2 1 1
Nuclear Periphery 0 0 0 0 0 0 1 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 2 0 0 0 2 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 1 1 0 2 0 4 3 4 0 1 0 0 0 2 4 2 0 4 0 0 1 1
Unique Cell Count 183 275 521 212 326 391 464 391 348 374 217 294 296 257 288 585 487 395 184 265 531 171 198 185
Labelled Cell Count 183 277 526 217 331 408 501 420 389 414 243 342 303 264 297 625 509 413 184 265 531 171 198 185


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 54.2 73.0 61.2 54.2 42.1 37.7 33.9 32.5 32.0 30.7 30.2 28.9 75.0 72.5 67.7 109.6 97.5 113.4 76.6 74.2 86.4
Std Deviation (1e-4) 10.2 14.9 11.1 10.8 8.2 7.9 7.5 8.3 7.5 7.6 8.2 7.4 16.7 16.8 15.2 35.0 29.9 30.6 17.9 25.8 25.4
Intensity Change (Log2) -0.18 -0.54 -0.7 -0.85 -0.91 -0.94 -1.0 -1.02 -1.09 0.29 0.24 0.14 0.84 0.67 0.89 0.32 0.28 0.5


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 4.5 4.0 5.7 6.5 0 6.6 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 2.9 5.1 4.8 7.5 6.3 5.5 7.1 0 0 0 1.3 0 0
Cytoplasm -1.3 -4.9 -4.9 -10.4 -11.6 -13.2 -13.4 -12.6 -13.2 1.3 1.1 0.4 1.3 0.4 -0.1
Endoplasmic Reticulum 0 4.1 3.2 7.6 9.1 9.9 7.8 8.3 8.1 0 0 0 3.6 1.8 2.1
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 5.0 4.0 0 7.3 8.5 7.6 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 48.9565 59.6847 59.7471 50.0162 54.4517 55.0058 44.61 69.0708 61.8508 52.2237 49.9745 66.4469 55.2123 70.9707 64.6447 62.8335 62.9644 66.7863
Actin 0.0067 0.0001 0.0009 0.0001 0.0002 0.0007 0.0047 0.0003 0.0056 0.0017 0.0002 0.0046 0.0112 0.0002 0.0025 0.0002 0.0059 0.0014
Bud 0.0018 0.0002 0.0003 0.0004 0.0005 0.0005 0.0026 0.0003 0.0007 0.0006 0.0006 0.0002 0.0008 0.0003 0.0007 0.0009 0.0008 0.0005
Bud Neck 0.0006 0.0002 0.0003 0.0004 0.0007 0.001 0.0006 0.0004 0.0004 0.0005 0.0008 0.0013 0.0005 0.0003 0.0009 0.0005 0.0005 0.0013
Bud Periphery 0.0015 0.0001 0.0001 0.0001 0.0003 0.0003 0.0039 0.0002 0.001 0.0005 0.0009 0.0002 0.0004 0.0002 0.0006 0.0004 0.0019 0.0006
Bud Site 0.0006 0.0001 0.0002 0.0001 0.0003 0.0001 0.002 0.0004 0.0004 0.001 0.0023 0.0001 0.0005 0.0002 0.002 0.0002 0.0008 0.0004
Cell Periphery 0.0051 0.0023 0.0031 0.0016 0.0026 0.0006 0.0152 0.0171 0.0112 0.0105 0.0053 0.005 0.0041 0.0049 0.0059 0.0009 0.0007 0.0024
Cytoplasm 0.8092 0.962 0.9166 0.8951 0.8528 0.9234 0.6865 0.9314 0.8681 0.7675 0.8251 0.8875 0.8237 0.9648 0.894 0.8603 0.9094 0.9396
Cytoplasmic Foci 0.0068 0.0009 0.0025 0.004 0.0085 0.0031 0.0112 0.0007 0.0061 0.0127 0.0104 0.0037 0.0028 0.0009 0.0022 0.0013 0.0026 0.0056
Eisosomes 0.001 0 0 0 0 0 0.0005 0 0.0001 0.0002 0.0002 0.0001 0.0001 0.0002 0.0002 0 0.0001 0.0001
Endoplasmic Reticulum 0.0533 0.0156 0.0214 0.0052 0.0113 0.0329 0.0597 0.0108 0.0093 0.0066 0.0057 0.0625 0.0577 0.007 0.0148 0.0039 0.0013 0.0124
Endosome 0.0094 0.0012 0.0064 0.0086 0.0183 0.0089 0.022 0.0007 0.0037 0.0145 0.0164 0.0043 0.0071 0.0014 0.0056 0.0052 0.0156 0.0087
Golgi 0.0018 0.0001 0.0019 0.0004 0.0019 0.0025 0.0031 0.0004 0.0006 0.0025 0.0027 0.0008 0.0007 0.0006 0.0055 0.0001 0.0075 0.0018
Lipid Particles 0.0023 0.0006 0.0019 0.0036 0.0034 0.0002 0.012 0.0015 0.0039 0.0116 0.0023 0.0015 0.0006 0.001 0.0014 0.0001 0.0048 0.0021
Mitochondria 0.0043 0.0001 0.0009 0.0001 0.0011 0.0029 0.0108 0.0001 0.0076 0.0082 0.0039 0.0007 0.0013 0.0024 0.0025 0.0005 0.0169 0.0024
None 0.0034 0.0002 0.001 0.0003 0.0017 0.0003 0.0175 0.0003 0.0018 0.0074 0.011 0.0011 0.0029 0.0002 0.0037 0.0005 0.0004 0.0007
Nuclear Periphery 0.0093 0.0009 0.0038 0.0027 0.0039 0.002 0.02 0.0018 0.006 0.0116 0.0048 0.0038 0.0126 0.0011 0.0042 0.0181 0.0016 0.0037
Nucleolus 0.0028 0 0.0002 0.0001 0.0104 0.0001 0.0073 0.0002 0.0012 0.0078 0.0089 0.0002 0.0027 0 0.0038 0.0002 0.0002 0.0005
Nucleus 0.0094 0.0008 0.0015 0.0015 0.0106 0.0014 0.0165 0.0015 0.0034 0.0069 0.0128 0.0019 0.0152 0.0009 0.0065 0.013 0.0013 0.0024
Peroxisomes 0.0003 0 0.0001 0 0.0001 0 0.0015 0 0.0008 0.0014 0.0011 0.0001 0.0001 0 0.0003 0 0.0013 0.0001
Punctate Nuclear 0.0009 0 0.0007 0.0001 0.0017 0 0.0054 0 0.0006 0.007 0.0009 0.0064 0.0032 0 0.001 0.0002 0.0002 0.0003
Vacuole 0.0583 0.0138 0.0326 0.0675 0.0607 0.0117 0.0843 0.0314 0.0623 0.1088 0.074 0.0124 0.0458 0.0125 0.0344 0.0841 0.0226 0.0101
Vacuole Periphery 0.0113 0.0007 0.0035 0.0081 0.0092 0.0072 0.0126 0.0007 0.0053 0.0106 0.0098 0.0014 0.0062 0.0009 0.0076 0.0093 0.0036 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 331.4869 341.2778 384.188 418.5677 424.3546 276.3964 315.188 323.2143 352.8491 347.8165
Translational Efficiency 1.8066 1.9176 1.7151 1.7353 1.7336 2.0615 1.8758 1.6666 1.6562 1.6998

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1117 189 795 1202 1393 1900 169 988 2510 2089 964 2190

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 3854.84 4790.02 6306.28 7464.83 3800.59 4586.98 7861.94 8167.39 3824.73 4605.35 6579.00 7781.78
Standard Deviation 830.06 1031.63 1147.32 1741.75 969.80 907.59 1440.64 1791.13 910.67 921.35 1341.38 1798.50
Intensity Change Log 2 0.313361 0.710118 0.953438 0.271321 1.048662 1.103652 0.292643 0.888103 1.029967

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000061 0.000206 0.000193 0.000404 0.000060 0.000082 0.000262 0.000238 0.000061 0.000093 0.000205 0.000329
Bud Neck 0.000286 0.002084 0.002916 0.002563 0.000380 0.000336 0.006941 0.002237 0.000338 0.000494 0.003622 0.002416
Bud Site 0.002097 0.012636 0.006522 0.008048 0.002618 0.002369 0.006001 0.009468 0.002386 0.003298 0.006431 0.008689
Cell Periphery 0.010500 0.028741 0.042255 0.065948 0.011043 0.008405 0.053769 0.062234 0.010802 0.010245 0.044274 0.064272
Cytoplasm 0.828675* 0.674027* 0.484445* 0.486657* 0.810014* 0.867467* 0.394365* 0.541199* 0.818318* 0.849966* 0.468653* 0.511263*
Cytoplasmic Foci 0.007367 0.017437 0.007951 0.004793 0.008936 0.006931 0.019667 0.003917 0.008238 0.007881 0.010005 0.004398
Eisosomes 0.000001 0.000003 0.000005 0.000010 0.000001 0.000001 0.000012 0.000007 0.000001 0.000001 0.000006 0.000009
Endoplasmic Reticulum 0.001145 0.000565 0.000899 0.000311 0.001075 0.000867 0.000278 0.000259 0.001106 0.000839 0.000790 0.000288
Endosome 0.000650 0.002510 0.000899 0.000900 0.000702 0.000585 0.001471 0.000509 0.000679 0.000760 0.000999 0.000723
Golgi 0.043381 0.055699 0.025505 0.010711 0.042858 0.033043 0.028978 0.008448 0.043091 0.035093 0.026114 0.009690
Lipid Particles 0.013324 0.029667 0.039387 0.031174 0.012929 0.010656 0.041825 0.045809 0.013105 0.012376 0.039815 0.037777
Mitochondria 0.000107 0.000108 0.000114 0.000977 0.000252 0.000150 0.000104 0.000479 0.000187 0.000146 0.000112 0.000752
Mitotic Spindle 0.000702 0.004098 0.001114 0.000488 0.001085 0.000687 0.004204 0.000644 0.000915 0.000995 0.001656 0.000558
None 0.001755 0.001473 0.000894 0.002092 0.003367 0.002271 0.001281 0.001370 0.002649 0.002199 0.000962 0.001767
Nuclear Periphery 0.000277 0.000563 0.001080 0.000766 0.000382 0.000285 0.002407 0.000615 0.000335 0.000310 0.001312 0.000698
Nuclear Periphery Foci 0.011067 0.010852 0.012704 0.006321 0.010249 0.008955 0.014765 0.005972 0.010613 0.009126 0.013065 0.006163
Nucleolus 0.000099 0.000107 0.000108 0.000199 0.000067 0.000104 0.000123 0.000167 0.000081 0.000104 0.000111 0.000184
Nucleus 0.004447 0.012164 0.014229 0.017314 0.007111 0.005906 0.038416 0.011888 0.005926 0.006472 0.018469 0.014866
Peroxisomes 0.000020 0.000067 0.000039 0.000106 0.000028 0.000017 0.000028 0.000145 0.000024 0.000022 0.000037 0.000124
Vacuole 0.040847 0.117014 0.320889* 0.336887* 0.042069 0.034402 0.348778* 0.291211* 0.041525 0.041876 0.325778* 0.316281*
Vacuole Periphery 0.033190 0.029979 0.037851 0.023331 0.044776 0.016482 0.036325 0.013184 0.039620 0.017703 0.037584 0.018753

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.90 -4.62 -5.48 -2.01 -2.84 -1.66 -6.14 -7.69 -6.22 0.75 -2.63 -6.09 -7.58 -6.33 -2.55
Bud Neck -3.79 -9.65 -7.98 -0.44 2.00 0.65 -6.99 -8.14 -8.73 4.86 -2.31 -11.60 -11.04 -9.95 4.39
Bud Site -4.10 -6.14 -6.11 1.23 -1.19 0.50 -2.86 -5.34 -5.77 -1.64 -2.01 -6.20 -7.94 -6.61 -1.83
Cell Periphery -3.84 -9.97 -16.07 -6.04 -5.33 2.34 -6.49 -13.91 -15.05 -1.29 0.69 -11.90 -21.26 -21.51 -5.64
Cytoplasm 6.65 24.18 27.17 6.73 -0.76 -6.28 16.35 20.92 27.25 -5.04 -3.92 28.97 34.09 37.12 -3.41
Cytoplasmic Foci -3.93 -2.43 1.66 4.38 3.37 2.13 -4.72 4.49 2.77 6.19 0.26 -3.87 4.20 3.53 6.52
Eisosomes -4.48 -8.21 -8.94 -5.47 -3.70 0.73 -5.92 -10.86 -11.02 2.67 -1.25 -9.90 -12.81 -12.32 -2.20
Endoplasmic Reticulum 2.03 0.99 3.01 2.16 2.45 0.74 4.58 4.86 2.60 0.03 1.03 1.76 5.30 2.59 2.55
Endosome -3.23 -3.33 -0.56 2.62 0.52 1.49 -1.14 1.88 0.84 1.49 -0.99 -2.93 0.02 0.46 1.80
Golgi -1.30 7.13 12.91 6.36 10.93 3.59 5.52 15.85 14.44 6.81 3.54 9.99 20.28 15.50 13.54
Lipid Particles -3.27 -9.82 -7.66 -0.01 3.38 1.46 -7.03 -10.88 -12.44 -0.26 0.63 -12.17 -13.22 -13.85 1.38
Mitochondria -0.12 -0.45 -1.84 -1.83 -1.81 0.96 1.47 -0.77 -1.20 -1.39 0.54 1.26 -1.92 -2.06 -2.21
Mitotic Spindle -2.33 -3.42 0.55 2.39 2.81 1.88 -1.25 1.69 0.37 1.50 -0.35 -1.73 1.80 2.04 2.58
None 0.66 1.83 -0.25 -0.81 -1.71 1.23 2.64 2.21 1.62 -0.70 0.74 3.50 1.50 0.96 -2.07
Nuclear Periphery -3.44 -10.76 -8.89 -1.38 4.05 2.04 -7.45 -3.39 -5.46 6.54 0.62 -12.13 -8.04 -8.41 7.46
Nuclear Periphery Foci -0.00 -1.22 4.69 3.56 8.23 1.47 -2.74 5.89 4.94 6.63 1.86 -2.58 7.39 5.78 10.18
Nucleolus -0.27 -0.54 -1.70 -3.06 -2.75 -0.60 -4.46 -3.72 -0.69 -1.13 -0.42 -1.71 -3.38 -1.12 -2.57
Nucleus -4.22 -8.17 -11.87 -3.04 -1.50 1.07 -6.81 -5.83 -7.73 4.97 -1.22 -9.67 -12.26 -12.30 1.82
Peroxisomes -3.22 -4.00 -2.71 -0.78 -1.89 1.36 -0.06 -1.56 -1.79 -1.56 0.55 -2.17 -2.62 -2.74 -2.18
Vacuole -5.63 -26.56 -34.23 -12.05 -0.75 2.24 -13.99 -28.23 -29.45 1.92 -0.51 -30.24 -44.36 -42.94 0.84
Vacuole Periphery 0.88 -0.30 4.66 1.50 5.28 9.39 2.91 11.18 3.52 4.39 10.25 2.28 11.15 0.58 7.98