Standard name
Human Ortholog
Description 2-enoyl thioester reductase; member of the medium chain dehydrogenase/reductase family; localized to mitochondria, where it has a probable role in fatty acid synthesis; human MECR functionally complements the respiratory growth defect of the null mutant
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.07 0.11 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0.06 0 0 0.05 0.05 0 0 0 0 0 0.08 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.1 0.07 0 0 0 0 0 0
Cytoplasm 0.73 0.79 0.71 0.77 0.62 0.48 0.34 0.38 0.28 0.24 0.24 0.2 0.94 0.95 0.96 0.26 0.16 0.18 0.27 0.6 0.18 0.22 0.3 0.33
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0 0 0 0 0 0 0
Endosome 0 0 0.06 0 0.06 0 0 0 0 0 0 0 0 0 0 0.21 0.22 0.18 0.05 0 0.06 0.05 0 0.05
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0.11 0 0 0 0 0 0 0
Mitochondria 0.4 0.21 0.16 0.25 0.33 0.52 0.66 0.58 0.76 0.83 0.75 0.81 0 0 0 0.18 0.11 0.11 0.51 0.26 0.57 0.55 0.48 0.4
Nucleus 0 0.05 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0.08 0 0.07 0 0.06 0.08 0 0 0 0 0 0 0 0.14 0.12 0.13 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0.05 0 0 0 0.05 0.07 0 0 0 0 0.09 0.19 0.31 0 0 0 0 0.05 0.05
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 1 0 0 0 0 0 0 0 0 0 0 0 5 5 2 6 8 5 0 1 9 3 7 10
Bud 2 0 8 10 10 13 39 17 15 29 22 24 4 2 0 4 9 1 1 0 5 1 5 9
Bud Neck 1 0 2 3 5 2 2 2 2 1 0 0 3 4 3 0 1 0 1 0 3 1 3 7
Bud Site 0 0 1 0 3 2 8 3 2 8 3 4 0 0 0 2 1 0 – – – – – –
Cell Periphery 0 0 4 5 5 1 5 2 2 3 3 5 4 3 3 8 12 3 1 0 2 0 2 5
Cytoplasm 106 15 191 233 263 223 222 254 98 144 107 115 388 400 463 25 18 8 46 42 58 27 113 138
Endoplasmic Reticulum 1 0 1 0 1 3 1 0 3 2 2 6 3 5 1 8 4 1 2 0 5 1 2 2
Endosome 0 0 15 6 24 12 12 25 2 2 9 0 5 7 7 20 25 8 8 0 18 6 16 20
Golgi 0 0 3 0 0 0 0 0 0 0 1 0 1 1 0 8 13 0 1 0 4 0 2 3
Mitochondria 59 4 44 75 139 242 432 386 265 506 336 463 6 1 1 17 13 5 86 18 179 67 179 170
Nucleus 0 1 0 1 0 6 10 10 3 7 7 4 0 0 0 4 0 0 0 0 0 1 2 1
Nuclear Periphery 0 0 0 0 0 2 3 4 3 10 9 13 0 0 0 1 1 0 0 0 0 0 0 0
Nucleolus 0 0 1 2 0 2 11 14 2 16 12 13 0 0 0 2 2 1 0 0 0 1 0 1
Peroxisomes 0 0 10 5 11 3 10 10 3 3 3 6 4 1 1 1 5 0 0 0 5 0 2 2
SpindlePole 3 0 21 12 31 19 39 50 15 20 14 17 5 6 3 13 14 6 6 1 13 2 9 15
Vac/Vac Membrane 1 0 3 3 13 25 26 26 14 29 31 23 2 2 6 9 22 14 7 2 1 4 19 23
Unique Cell Count 146 19 270 303 423 466 653 664 350 612 446 575 414 423 484 95 116 45 170 71 317 124 377 425
Labelled Cell Count 174 20 304 355 505 555 820 803 429 780 559 693 430 437 490 128 148 52 170 71 317 124 377 425


Mitochondria

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.1 3.8 4.1 4.0 4.8 3.9 3.8 3.7 3.8 3.8 4.6 4.2 5.0 5.0 5.1 7.7 7.8 7.5 4.6 5.4 5.8
Std Deviation (1e-4) 0.4 0.8 1.9 4.3 6.7 2.7 1.9 1.8 2.0 1.7 4.9 2.4 1.0 1.5 1.3 2.4 1.6 1.9 0.8 2.0 2.2
Intensity Change (Log2) – – – -0.02 0.24 -0.05 -0.11 -0.12 -0.11 -0.08 0.19 0.06 0.3 0.31 0.33 0.91 0.94 0.88 0.17 0.4 0.51


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin – – – – – – – – – – – – – – –
Bud – – – – – – – – – – – – – 0 –
Bud Neck – – – – – – – – – – – – – 0 –
Bud Site – – – – – – – – – – – – – 0 –
Cell Periphery – – – – – – – – – – – – – 0 –
Cyto – – – – – – – – – – – – – – –
Endoplasmic Reticulum – – – – – – – – – – – – – 0 –
Endosome – – – – – – – – – – – – – 0 –
Golgi – – – – – – – – – – – – – 0 –
Mitochondria – – – – – – – – – – – – – 0 –
Nuclear Periphery – – – – – – – – – – – – – 0 –
Nuc – – – – – – – – – – – – – – –
Nucleolus – – – – – – – – – – – – – 0 –
Peroxisomes – – – – – – – – – – – – – 0 –
SpindlePole – – – – – – – – – – – – – 0 –
Vac – – – – – – – – – – – – – – –
Cortical Patches – – – – – – – – – – – – – 0 –
Cytoplasm – – – – – – – – – – – – – 0 –
Nucleus – – – – – – – – – – – – – 0 –
Vacuole – – – – – – – – – – – – – 6.5 –

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration -0.2052 -0.0439 -0.1957 -0.237 -0.7275 -0.0927 -0.8735 -0.1856 -0.7551 -0.7772 -0.3779 -0.5329 -1.9412 -1.5342 -1.6529 -1.6936 -1.4853 -1.7403
Actin 0.0218 0.0081 0.0034 0.0334 0.0096 0.0026 0.0578 0.0215 0.006 0.0084 0.0507 0.009 0.0183 0.0063 0.0052 0.0209 0.0359 0.0093
Bud 0.0041 0.0046 0.0019 0.0066 0.0012 0.001 0.001 0.007 0.0028 0.0031 0.0178 0.0015 0.0015 0.0027 0.0015 0.0035 0.0033 0.0005
Bud Neck 0.0024 0.0013 0.0019 0.0013 0.0014 0.0071 0.0024 0.0016 0.0016 0.0033 0.0023 0.0103 0.0014 0.0023 0.0028 0.0039 0.0027 0.0067
Bud Periphery 0.0037 0.018 0.0042 0.0228 0.0049 0.0024 0.0019 0.0107 0.0073 0.0055 0.0253 0.0037 0.0022 0.0043 0.0035 0.0066 0.0072 0.0007
Bud Site 0.0072 0.018 0.0091 0.0048 0.0015 0.0006 0.0063 0.0431 0.0038 0.0121 0.0052 0.0015 0.0103 0.0215 0.0038 0.0075 0.0061 0.0006
Cell Periphery 0.0014 0.0013 0.0013 0.0009 0.0007 0.0005 0.0006 0.0009 0.0008 0.001 0.0007 0.0007 0.0008 0.0015 0.0028 0.0009 0.0009 0.0002
Cytoplasm 0.0803 0.117 0.0948 0.1608 0.0978 0.129 0.1374 0.2065 0.1849 0.2216 0.095 0.2507 0.1647 0.2366 0.2116 0.2482 0.1239 0.3003
Cytoplasmic Foci 0.1228 0.1189 0.1466 0.0942 0.1475 0.1492 0.1121 0.1349 0.1167 0.1498 0.0569 0.1259 0.0995 0.1318 0.0757 0.1342 0.1152 0.1093
Eisosomes 0.0008 0.0004 0.0008 0.0004 0.0011 0.0003 0.0009 0.0003 0.0003 0.0003 0.0005 0.0002 0.0004 0.0002 0.0005 0.0003 0.001 0.0001
Endoplasmic Reticulum 0.0077 0.0039 0.0042 0.009 0.0038 0.0018 0.0075 0.0059 0.0075 0.0042 0.0109 0.0077 0.0067 0.0071 0.0084 0.0084 0.0071 0.0049
Endosome 0.1464 0.0982 0.1057 0.1212 0.075 0.0797 0.2068 0.1309 0.1551 0.1492 0.1496 0.1413 0.1179 0.097 0.0907 0.1271 0.0874 0.0883
Golgi 0.0435 0.0292 0.0302 0.0217 0.0175 0.0247 0.0449 0.0353 0.0411 0.0356 0.0492 0.0389 0.0338 0.0445 0.0239 0.0249 0.0398 0.028
Lipid Particles 0.0296 0.0107 0.0103 0.0057 0.0148 0.0097 0.0517 0.0309 0.013 0.0116 0.0089 0.0168 0.0227 0.011 0.0112 0.0037 0.0589 0.0046
Mitochondria 0.1342 0.1565 0.2007 0.2318 0.0987 0.1575 0.0943 0.1214 0.1929 0.1578 0.1911 0.1731 0.0712 0.0922 0.0714 0.0826 0.0501 0.0769
None 0.3177 0.3833 0.3295 0.2439 0.4698 0.3903 0.1961 0.1999 0.22 0.1875 0.2902 0.1785 0.3973 0.3143 0.4375 0.2908 0.4139 0.3362
Nuclear Periphery 0.0034 0.0014 0.002 0.0054 0.014 0.0006 0.0087 0.0028 0.003 0.0017 0.0133 0.0021 0.0042 0.0014 0.0055 0.0018 0.0054 0.0015
Nucleolus 0.0012 0.0012 0.0009 0.001 0.0024 0.0007 0.0021 0.0012 0.0005 0.0008 0.0008 0.001 0.0014 0.0005 0.0012 0.0002 0.0027 0.0002
Nucleus 0.0021 0.0015 0.0016 0.0035 0.0048 0.0012 0.0046 0.0033 0.0018 0.0016 0.0067 0.0026 0.0026 0.0014 0.0059 0.0016 0.0021 0.0014
Peroxisomes 0.0418 0.0161 0.0336 0.0114 0.0136 0.0328 0.0276 0.02 0.0205 0.0241 0.0087 0.0189 0.0129 0.0095 0.0152 0.019 0.0214 0.0184
Punctate Nuclear 0.0083 0.0021 0.0018 0.0041 0.0095 0.0033 0.0128 0.0017 0.0041 0.0038 0.0037 0.0024 0.0108 0.0011 0.0056 0.0025 0.0046 0.0029
Vacuole 0.0142 0.0067 0.0115 0.0112 0.0059 0.0035 0.016 0.0166 0.0114 0.011 0.0078 0.0089 0.0165 0.0097 0.0126 0.0072 0.007 0.0059
Vacuole Periphery 0.0052 0.0018 0.0041 0.0048 0.0045 0.0016 0.0065 0.0037 0.0052 0.0062 0.0049 0.0045 0.0032 0.0029 0.0035 0.0042 0.0035 0.003

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 36.6836 28.4781 19.2109 39.7218 34.4198 35.6985 28.1397 36.2181 43.6518 41.6232
Translational Efficiency 1.6949 1.3642 2.3648 1.5053 1.5344 2.0879 1.8713 1.4574 1.717 1.6938

Hit Data

Dataset Hit
Protein Abundance ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘