Standard name | |
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Human Ortholog | |
Description | 2-enoyl thioester reductase; member of the medium chain dehydrogenase/reductase family; localized to mitochondria, where it has a probable role in fatty acid synthesis; human MECR functionally complements the respiratory growth defect of the null mutant |
ensLOC | DeepLoc | |||||||||||||||||||||||
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Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 2 | 6 | 8 | 5 | 0 | 1 | 9 | 3 | 7 | 10 |
Bud | 2 | 0 | 8 | 10 | 10 | 13 | 39 | 17 | 15 | 29 | 22 | 24 | 4 | 2 | 0 | 4 | 9 | 1 | 1 | 0 | 5 | 1 | 5 | 9 |
Bud Neck | 1 | 0 | 2 | 3 | 5 | 2 | 2 | 2 | 2 | 1 | 0 | 0 | 3 | 4 | 3 | 0 | 1 | 0 | 1 | 0 | 3 | 1 | 3 | 7 |
Bud Site | 0 | 0 | 1 | 0 | 3 | 2 | 8 | 3 | 2 | 8 | 3 | 4 | 0 | 0 | 0 | 2 | 1 | 0 | – | – | – | – | – | – |
Cell Periphery | 0 | 0 | 4 | 5 | 5 | 1 | 5 | 2 | 2 | 3 | 3 | 5 | 4 | 3 | 3 | 8 | 12 | 3 | 1 | 0 | 2 | 0 | 2 | 5 |
Cytoplasm | 106 | 15 | 191 | 233 | 263 | 223 | 222 | 254 | 98 | 144 | 107 | 115 | 388 | 400 | 463 | 25 | 18 | 8 | 46 | 42 | 58 | 27 | 113 | 138 |
Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 1 | 3 | 1 | 0 | 3 | 2 | 2 | 6 | 3 | 5 | 1 | 8 | 4 | 1 | 2 | 0 | 5 | 1 | 2 | 2 |
Endosome | 0 | 0 | 15 | 6 | 24 | 12 | 12 | 25 | 2 | 2 | 9 | 0 | 5 | 7 | 7 | 20 | 25 | 8 | 8 | 0 | 18 | 6 | 16 | 20 |
Golgi | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 8 | 13 | 0 | 1 | 0 | 4 | 0 | 2 | 3 |
Mitochondria | 59 | 4 | 44 | 75 | 139 | 242 | 432 | 386 | 265 | 506 | 336 | 463 | 6 | 1 | 1 | 17 | 13 | 5 | 86 | 18 | 179 | 67 | 179 | 170 |
Nucleus | 0 | 1 | 0 | 1 | 0 | 6 | 10 | 10 | 3 | 7 | 7 | 4 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 3 | 10 | 9 | 13 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 1 | 2 | 0 | 2 | 11 | 14 | 2 | 16 | 12 | 13 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 1 |
Peroxisomes | 0 | 0 | 10 | 5 | 11 | 3 | 10 | 10 | 3 | 3 | 3 | 6 | 4 | 1 | 1 | 1 | 5 | 0 | 0 | 0 | 5 | 0 | 2 | 2 |
SpindlePole | 3 | 0 | 21 | 12 | 31 | 19 | 39 | 50 | 15 | 20 | 14 | 17 | 5 | 6 | 3 | 13 | 14 | 6 | 6 | 1 | 13 | 2 | 9 | 15 |
Vac/Vac Membrane | 1 | 0 | 3 | 3 | 13 | 25 | 26 | 26 | 14 | 29 | 31 | 23 | 2 | 2 | 6 | 9 | 22 | 14 | 7 | 2 | 1 | 4 | 19 | 23 |
Unique Cell Count | 146 | 19 | 270 | 303 | 423 | 466 | 653 | 664 | 350 | 612 | 446 | 575 | 414 | 423 | 484 | 95 | 116 | 45 | 170 | 71 | 317 | 124 | 377 | 425 |
Labelled Cell Count | 174 | 20 | 304 | 355 | 505 | 555 | 820 | 803 | 429 | 780 | 559 | 693 | 430 | 437 | 490 | 128 | 148 | 52 | 170 | 71 | 317 | 124 | 377 | 425 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 3.1 | 3.8 | 4.1 | 4.0 | 4.8 | 3.9 | 3.8 | 3.7 | 3.8 | 3.8 | 4.6 | 4.2 | 5.0 | 5.0 | 5.1 | 7.7 | 7.8 | 7.5 | 4.6 | 5.4 | 5.8 |
Std Deviation (1e-4) | 0.4 | 0.8 | 1.9 | 4.3 | 6.7 | 2.7 | 1.9 | 1.8 | 2.0 | 1.7 | 4.9 | 2.4 | 1.0 | 1.5 | 1.3 | 2.4 | 1.6 | 1.9 | 0.8 | 2.0 | 2.2 |
Intensity Change (Log2) | – | – | – | -0.02 | 0.24 | -0.05 | -0.11 | -0.12 | -0.11 | -0.08 | 0.19 | 0.06 | 0.3 | 0.31 | 0.33 | 0.91 | 0.94 | 0.88 | 0.17 | 0.4 | 0.51 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 6.5 | – |
R1 | R2 | R3 | ||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Pre-START | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Concentration | -0.2052 | -0.0439 | -0.1957 | -0.237 | -0.7275 | -0.0927 | -0.8735 | -0.1856 | -0.7551 | -0.7772 | -0.3779 | -0.5329 | -1.9412 | -1.5342 | -1.6529 | -1.6936 | -1.4853 | -1.7403 |
Actin | 0.0218 | 0.0081 | 0.0034 | 0.0334 | 0.0096 | 0.0026 | 0.0578 | 0.0215 | 0.006 | 0.0084 | 0.0507 | 0.009 | 0.0183 | 0.0063 | 0.0052 | 0.0209 | 0.0359 | 0.0093 |
Bud | 0.0041 | 0.0046 | 0.0019 | 0.0066 | 0.0012 | 0.001 | 0.001 | 0.007 | 0.0028 | 0.0031 | 0.0178 | 0.0015 | 0.0015 | 0.0027 | 0.0015 | 0.0035 | 0.0033 | 0.0005 |
Bud Neck | 0.0024 | 0.0013 | 0.0019 | 0.0013 | 0.0014 | 0.0071 | 0.0024 | 0.0016 | 0.0016 | 0.0033 | 0.0023 | 0.0103 | 0.0014 | 0.0023 | 0.0028 | 0.0039 | 0.0027 | 0.0067 |
Bud Periphery | 0.0037 | 0.018 | 0.0042 | 0.0228 | 0.0049 | 0.0024 | 0.0019 | 0.0107 | 0.0073 | 0.0055 | 0.0253 | 0.0037 | 0.0022 | 0.0043 | 0.0035 | 0.0066 | 0.0072 | 0.0007 |
Bud Site | 0.0072 | 0.018 | 0.0091 | 0.0048 | 0.0015 | 0.0006 | 0.0063 | 0.0431 | 0.0038 | 0.0121 | 0.0052 | 0.0015 | 0.0103 | 0.0215 | 0.0038 | 0.0075 | 0.0061 | 0.0006 |
Cell Periphery | 0.0014 | 0.0013 | 0.0013 | 0.0009 | 0.0007 | 0.0005 | 0.0006 | 0.0009 | 0.0008 | 0.001 | 0.0007 | 0.0007 | 0.0008 | 0.0015 | 0.0028 | 0.0009 | 0.0009 | 0.0002 |
Cytoplasm | 0.0803 | 0.117 | 0.0948 | 0.1608 | 0.0978 | 0.129 | 0.1374 | 0.2065 | 0.1849 | 0.2216 | 0.095 | 0.2507 | 0.1647 | 0.2366 | 0.2116 | 0.2482 | 0.1239 | 0.3003 |
Cytoplasmic Foci | 0.1228 | 0.1189 | 0.1466 | 0.0942 | 0.1475 | 0.1492 | 0.1121 | 0.1349 | 0.1167 | 0.1498 | 0.0569 | 0.1259 | 0.0995 | 0.1318 | 0.0757 | 0.1342 | 0.1152 | 0.1093 |
Eisosomes | 0.0008 | 0.0004 | 0.0008 | 0.0004 | 0.0011 | 0.0003 | 0.0009 | 0.0003 | 0.0003 | 0.0003 | 0.0005 | 0.0002 | 0.0004 | 0.0002 | 0.0005 | 0.0003 | 0.001 | 0.0001 |
Endoplasmic Reticulum | 0.0077 | 0.0039 | 0.0042 | 0.009 | 0.0038 | 0.0018 | 0.0075 | 0.0059 | 0.0075 | 0.0042 | 0.0109 | 0.0077 | 0.0067 | 0.0071 | 0.0084 | 0.0084 | 0.0071 | 0.0049 |
Endosome | 0.1464 | 0.0982 | 0.1057 | 0.1212 | 0.075 | 0.0797 | 0.2068 | 0.1309 | 0.1551 | 0.1492 | 0.1496 | 0.1413 | 0.1179 | 0.097 | 0.0907 | 0.1271 | 0.0874 | 0.0883 |
Golgi | 0.0435 | 0.0292 | 0.0302 | 0.0217 | 0.0175 | 0.0247 | 0.0449 | 0.0353 | 0.0411 | 0.0356 | 0.0492 | 0.0389 | 0.0338 | 0.0445 | 0.0239 | 0.0249 | 0.0398 | 0.028 |
Lipid Particles | 0.0296 | 0.0107 | 0.0103 | 0.0057 | 0.0148 | 0.0097 | 0.0517 | 0.0309 | 0.013 | 0.0116 | 0.0089 | 0.0168 | 0.0227 | 0.011 | 0.0112 | 0.0037 | 0.0589 | 0.0046 |
Mitochondria | 0.1342 | 0.1565 | 0.2007 | 0.2318 | 0.0987 | 0.1575 | 0.0943 | 0.1214 | 0.1929 | 0.1578 | 0.1911 | 0.1731 | 0.0712 | 0.0922 | 0.0714 | 0.0826 | 0.0501 | 0.0769 |
None | 0.3177 | 0.3833 | 0.3295 | 0.2439 | 0.4698 | 0.3903 | 0.1961 | 0.1999 | 0.22 | 0.1875 | 0.2902 | 0.1785 | 0.3973 | 0.3143 | 0.4375 | 0.2908 | 0.4139 | 0.3362 |
Nuclear Periphery | 0.0034 | 0.0014 | 0.002 | 0.0054 | 0.014 | 0.0006 | 0.0087 | 0.0028 | 0.003 | 0.0017 | 0.0133 | 0.0021 | 0.0042 | 0.0014 | 0.0055 | 0.0018 | 0.0054 | 0.0015 |
Nucleolus | 0.0012 | 0.0012 | 0.0009 | 0.001 | 0.0024 | 0.0007 | 0.0021 | 0.0012 | 0.0005 | 0.0008 | 0.0008 | 0.001 | 0.0014 | 0.0005 | 0.0012 | 0.0002 | 0.0027 | 0.0002 |
Nucleus | 0.0021 | 0.0015 | 0.0016 | 0.0035 | 0.0048 | 0.0012 | 0.0046 | 0.0033 | 0.0018 | 0.0016 | 0.0067 | 0.0026 | 0.0026 | 0.0014 | 0.0059 | 0.0016 | 0.0021 | 0.0014 |
Peroxisomes | 0.0418 | 0.0161 | 0.0336 | 0.0114 | 0.0136 | 0.0328 | 0.0276 | 0.02 | 0.0205 | 0.0241 | 0.0087 | 0.0189 | 0.0129 | 0.0095 | 0.0152 | 0.019 | 0.0214 | 0.0184 |
Punctate Nuclear | 0.0083 | 0.0021 | 0.0018 | 0.0041 | 0.0095 | 0.0033 | 0.0128 | 0.0017 | 0.0041 | 0.0038 | 0.0037 | 0.0024 | 0.0108 | 0.0011 | 0.0056 | 0.0025 | 0.0046 | 0.0029 |
Vacuole | 0.0142 | 0.0067 | 0.0115 | 0.0112 | 0.0059 | 0.0035 | 0.016 | 0.0166 | 0.0114 | 0.011 | 0.0078 | 0.0089 | 0.0165 | 0.0097 | 0.0126 | 0.0072 | 0.007 | 0.0059 |
Vacuole Periphery | 0.0052 | 0.0018 | 0.0041 | 0.0048 | 0.0045 | 0.0016 | 0.0065 | 0.0037 | 0.0052 | 0.0062 | 0.0049 | 0.0045 | 0.0032 | 0.0029 | 0.0035 | 0.0042 | 0.0035 | 0.003 |
R1 | R2 | |||||||||
---|---|---|---|---|---|---|---|---|---|---|
G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | G1 Post-START | S/G2 | Metaphase | Anaphase | Telophase | |
Gene Expression | 36.6836 | 28.4781 | 19.2109 | 39.7218 | 34.4198 | 35.6985 | 28.1397 | 36.2181 | 43.6518 | 41.6232 |
Translational Efficiency | 1.6949 | 1.3642 | 2.3648 | 1.5053 | 1.5344 | 2.0879 | 1.8713 | 1.4574 | 1.717 | 1.6938 |
Dataset | Hit |
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Protein Abundance | ✘ |
Protein Localization | ✘ |
Gene Expression | ✘ |
Translational Efficiency | ✘ |