| Standard name | |
|---|---|
| Description | 2-enoyl thioester reductase; member of the medium chain dehydrogenase/reductase family; localized to mitochondria, where it has a probable role in fatty acid synthesis; human MECR functionally complements the respiratory growth defect of the null mutant |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 5 | 5 | 2 | 6 | 8 | 5 | 0 | 1 | 9 | 3 | 7 | 10 |
| Bud | 2 | 0 | 8 | 10 | 10 | 13 | 39 | 17 | 15 | 29 | 22 | 24 | 4 | 2 | 0 | 4 | 9 | 1 | 1 | 0 | 5 | 1 | 5 | 9 |
| Bud Neck | 1 | 0 | 2 | 3 | 5 | 2 | 2 | 2 | 2 | 1 | 0 | 0 | 3 | 4 | 3 | 0 | 1 | 0 | 1 | 0 | 3 | 1 | 3 | 7 |
| Bud Site | 0 | 0 | 1 | 0 | 3 | 2 | 8 | 3 | 2 | 8 | 3 | 4 | 0 | 0 | 0 | 2 | 1 | 0 | – | – | – | – | – | – |
| Cell Periphery | 0 | 0 | 4 | 5 | 5 | 1 | 5 | 2 | 2 | 3 | 3 | 5 | 4 | 3 | 3 | 8 | 12 | 3 | 1 | 0 | 2 | 0 | 2 | 5 |
| Cytoplasm | 106 | 15 | 191 | 233 | 263 | 223 | 222 | 254 | 98 | 144 | 107 | 115 | 388 | 400 | 463 | 25 | 18 | 8 | 46 | 42 | 58 | 27 | 113 | 138 |
| Endoplasmic Reticulum | 1 | 0 | 1 | 0 | 1 | 3 | 1 | 0 | 3 | 2 | 2 | 6 | 3 | 5 | 1 | 8 | 4 | 1 | 2 | 0 | 5 | 1 | 2 | 2 |
| Endosome | 0 | 0 | 15 | 6 | 24 | 12 | 12 | 25 | 2 | 2 | 9 | 0 | 5 | 7 | 7 | 20 | 25 | 8 | 8 | 0 | 18 | 6 | 16 | 20 |
| Golgi | 0 | 0 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 1 | 0 | 8 | 13 | 0 | 1 | 0 | 4 | 0 | 2 | 3 |
| Mitochondria | 59 | 4 | 44 | 75 | 139 | 242 | 432 | 386 | 265 | 506 | 336 | 463 | 6 | 1 | 1 | 17 | 13 | 5 | 86 | 18 | 179 | 67 | 179 | 170 |
| Nucleus | 0 | 1 | 0 | 1 | 0 | 6 | 10 | 10 | 3 | 7 | 7 | 4 | 0 | 0 | 0 | 4 | 0 | 0 | 0 | 0 | 0 | 1 | 2 | 1 |
| Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 2 | 3 | 4 | 3 | 10 | 9 | 13 | 0 | 0 | 0 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | 0 | 0 | 1 | 2 | 0 | 2 | 11 | 14 | 2 | 16 | 12 | 13 | 0 | 0 | 0 | 2 | 2 | 1 | 0 | 0 | 0 | 1 | 0 | 1 |
| Peroxisomes | 0 | 0 | 10 | 5 | 11 | 3 | 10 | 10 | 3 | 3 | 3 | 6 | 4 | 1 | 1 | 1 | 5 | 0 | 0 | 0 | 5 | 0 | 2 | 2 |
| SpindlePole | 3 | 0 | 21 | 12 | 31 | 19 | 39 | 50 | 15 | 20 | 14 | 17 | 5 | 6 | 3 | 13 | 14 | 6 | 6 | 1 | 13 | 2 | 9 | 15 |
| Vac/Vac Membrane | 1 | 0 | 3 | 3 | 13 | 25 | 26 | 26 | 14 | 29 | 31 | 23 | 2 | 2 | 6 | 9 | 22 | 14 | 7 | 2 | 1 | 4 | 19 | 23 |
| Unique Cell Count | 146 | 19 | 270 | 303 | 423 | 466 | 653 | 664 | 350 | 612 | 446 | 575 | 414 | 423 | 484 | 95 | 116 | 45 | 170 | 71 | 317 | 124 | 377 | 425 |
| Labelled Cell Count | 174 | 20 | 304 | 355 | 505 | 555 | 820 | 803 | 429 | 780 | 559 | 693 | 430 | 437 | 490 | 128 | 148 | 52 | 170 | 71 | 317 | 124 | 377 | 425 |
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | 3.1 | 3.8 | 4.1 | 4.0 | 4.8 | 3.9 | 3.8 | 3.7 | 3.8 | 3.8 | 4.6 | 4.2 | 5.0 | 5.0 | 5.1 | 7.7 | 7.8 | 7.5 | 4.6 | 5.4 | 5.8 |
| Std Deviation (1e-4) | 0.4 | 0.8 | 1.9 | 4.3 | 6.7 | 2.7 | 1.9 | 1.8 | 2.0 | 1.7 | 4.9 | 2.4 | 1.0 | 1.5 | 1.3 | 2.4 | 1.6 | 1.9 | 0.8 | 2.0 | 2.2 |
| Intensity Change (Log2) | – | – | – | -0.02 | 0.24 | -0.05 | -0.11 | -0.12 | -0.11 | -0.08 | 0.19 | 0.06 | 0.3 | 0.31 | 0.33 | 0.91 | 0.94 | 0.88 | 0.17 | 0.4 | 0.51 |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | 0 | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | 6.5 | – |
External localization resources
Endocytosis
| Temp | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Vph1-GFP) |
|---|---|---|---|---|
| 37℃ | ||||
| RT |
Cell Cycle Omics
CYCLoPs (Etr1-GFP)
| Gene / Allele | Actin Patch (Sac6-tdTomato) | Cortical Patch (Sla1-GFP) | Late Endosome (Snf7-GFP) | Vacuole (Sac6-tdTomato) |
|---|
| Gene | Images |
|---|
| Gene | Images |
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Images are not yet available
Images are not yet available
