Standard name
Human Ortholog
Description AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner; directly phosphorylated by TORC1; phosphorylates ribosomal protein Rps6a/b (S6), in a TORC-dependent manner; undergoes autophosphorylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.96 1.0 0.97 0.94 0.95 0.94 0.85 0.8 0.75 0.74 0.76 0.69 0.98 0.99 0.99 0.94 0.97 0.95 0.94 0.96 0.87 0.92 0.94 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0.28 0.26 0.36 0.43 0.38 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 3 3 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 2 0 5 2 1 1 6 9 6 9 8 4 7 1 1 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 3 4 4 4 10 7 7 8 14 3 5 15 10 19 7 2 5 0 0 0 0 0 0
Cytoplasm 268 267 259 174 307 293 283 411 246 313 136 107 594 713 743 246 117 99 265 255 249 434 482 410
Endoplasmic Reticulum 1 2 2 3 7 2 2 6 6 7 1 6 6 4 4 10 2 3 4 1 9 13 12 19
Endosome 1 0 0 0 0 0 0 2 1 1 0 0 1 0 0 2 1 1 1 0 0 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 1 4 2
Mitochondria 8 0 4 7 8 52 93 133 117 180 68 75 1 0 0 6 0 1 1 0 2 2 2 3
Nucleus 0 1 8 6 1 1 2 4 2 0 0 0 3 2 4 3 2 1 0 2 0 1 1 0
Nuclear Periphery 0 1 0 0 0 0 3 1 2 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1
Nucleolus 2 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 1 0 1 5 1 1 8 2 4 4 1 6 3 2 10 1 2 2 2 19 11 5 14
Unique Cell Count 279 268 267 186 323 312 331 511 326 422 179 154 607 723 751 261 121 104 283 265 286 472 515 466
Labelled Cell Count 289 275 282 197 333 361 398 583 393 531 220 198 633 733 773 286 125 113 283 265 286 472 515 466


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 9.7 8.4 8.0 7.5 6.3 5.1 5.2 5.0 4.6 4.7 4.4 10.8 10.4 10.5 9.7 10.5 12.4 10.0 9.8 10.5
Std Deviation (1e-4) 1.5 2.0 1.6 1.4 1.4 1.4 1.3 1.2 1.2 1.0 1.0 1.4 2.1 2.1 2.0 2.1 2.1 2.8 1.8 1.7 1.7
Intensity Change (Log2) – – – -0.07 -0.15 -0.42 -0.72 -0.69 -0.74 -0.87 -0.85 -0.93 0.36 0.31 0.32 0.21 0.33 0.57 0.26 0.22 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.3 0 0 0.8 0 0 0 0.9 0 1.0 1.0 0 0
Cytoplasm -1.8 -1.2 -1.8 -4.8 -6.3 -7.3 -7.8 -6.8 -8.1 0.8 1.7 2.2 -1.5 -0.2 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.8 6.2 8.8 8.5 10.3 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0 0 0 0 0 0 0 0 0 0 0 -1.5 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.7395 11.0763 10.1812 9.1608 8.9698 9.7568 3.4761 6.0233 5.481 4.8794 4.2004 5.3093 5.7927 8.7389 7.7979 6.9608 7.6942 7.7508
Actin 0.0131 0.0022 0.0094 0.0011 0.0166 0.0045 0.0257 0.0005 0.0077 0.0559 0.0114 0.0074 0.0139 0.0003 0.0081 0.017 0.0011 0.0023
Bud 0.0007 0.0006 0.0006 0.0008 0.0023 0.0004 0.0009 0.0002 0.0049 0.0021 0.0007 0.0007 0.0003 0.0001 0.0006 0.0007 0.0003 0.0002
Bud Neck 0.0054 0.0003 0.0025 0.0078 0.0014 0.0012 0.006 0.0002 0.0005 0.0006 0.0006 0.0031 0.0011 0.0001 0.0006 0.0036 0.0276 0.0012
Bud Periphery 0.0009 0.0005 0.0011 0.0003 0.0029 0.0003 0.0009 0.0001 0.0014 0.0012 0.0006 0.0005 0.0002 0 0.0004 0.0008 0.0004 0.0003
Bud Site 0.0033 0.0036 0.0022 0.0081 0.0229 0.0002 0.0044 0.0011 0.0144 0.0022 0.0012 0.0003 0.0029 0.0013 0.0133 0.0074 0.0003 0.0003
Cell Periphery 0.0008 0.0006 0.001 0.0003 0.0004 0.0003 0.0003 0.0003 0.0005 0.0003 0.0001 0.0001 0.0003 0.0005 0.0005 0.0007 0.0003 0.0003
Cytoplasm 0.7455 0.9725 0.8783 0.9033 0.7483 0.9435 0.6687 0.9424 0.8626 0.8028 0.7677 0.893 0.819 0.9846 0.9219 0.8233 0.9043 0.9633
Cytoplasmic Foci 0.0313 0.0031 0.0166 0.0198 0.0142 0.0063 0.0575 0.0064 0.0277 0.0171 0.0535 0.0165 0.042 0.0039 0.0128 0.0269 0.0073 0.005
Eisosomes 0.0009 0.0001 0.0001 0 0.0002 0 0.0003 0.0002 0.0001 0.0004 0.0001 0 0.0001 0 0 0.0015 0 0
Endoplasmic Reticulum 0.0576 0.0034 0.0095 0.0059 0.0035 0.0156 0.0165 0.0038 0.0062 0.0021 0.0044 0.0142 0.013 0.0022 0.0065 0.0026 0.0019 0.0119
Endosome 0.0331 0.0029 0.0239 0.0211 0.1006 0.0088 0.0675 0.0043 0.0258 0.0395 0.0293 0.0314 0.0317 0.0017 0.013 0.0281 0.0106 0.0068
Golgi 0.01 0.0006 0.0098 0.0023 0.017 0.0032 0.0306 0.0005 0.0081 0.0137 0.0288 0.0176 0.0129 0.0003 0.0043 0.0125 0.0077 0.0018
Lipid Particles 0.0048 0.0001 0.0045 0.0024 0.0037 0.0033 0.0305 0.0001 0.0077 0.0108 0.0245 0.0012 0.0168 0.0001 0.001 0.0076 0.0147 0.0001
Mitochondria 0.0263 0.0004 0.0055 0.001 0.0237 0.0048 0.0122 0.0001 0.0087 0.0365 0.0513 0.0072 0.0045 0 0.002 0.0289 0.0144 0.0036
None 0.0172 0.0041 0.0098 0.0018 0.0009 0.0022 0.0254 0.0368 0.0113 0.0014 0.0045 0.0023 0.0173 0.0029 0.008 0.0033 0.0022 0.0008
Nuclear Periphery 0.0079 0.0005 0.0062 0.0013 0.0039 0.0007 0.0025 0.0003 0.0017 0.0014 0.0006 0.0009 0.0019 0.0003 0.0007 0.0007 0.0004 0.0004
Nucleolus 0.0011 0 0.0006 0.0009 0.0005 0 0.0009 0.0001 0.0003 0.0002 0.0001 0 0.0004 0 0.0002 0.0007 0.0001 0
Nucleus 0.0215 0.001 0.0042 0.0022 0.0017 0.0009 0.002 0.0004 0.0011 0.0008 0.0006 0.0005 0.0011 0.0004 0.0008 0.0012 0.0006 0.0005
Peroxisomes 0.004 0 0.0021 0.0023 0.003 0.001 0.0311 0 0.0057 0.0011 0.017 0.0008 0.0104 0 0.0012 0.025 0.0023 0.0001
Punctate Nuclear 0.0035 0.0001 0.0013 0.001 0.0006 0.0001 0.0072 0.0001 0.0008 0.0002 0.0007 0.0003 0.0057 0.0001 0.0003 0.0004 0.0001 0
Vacuole 0.0094 0.0027 0.0092 0.0145 0.023 0.002 0.0072 0.0021 0.0024 0.0072 0.0016 0.0014 0.0039 0.0009 0.0033 0.006 0.0027 0.0008
Vacuole Periphery 0.0017 0.0004 0.0015 0.0017 0.0087 0.0006 0.002 0.0001 0.0006 0.0027 0.0007 0.0006 0.0006 0.0001 0.0003 0.0011 0.0007 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.9208 16.2521 14.8428 15.9251 18.132 18.1702 23.8587 22.3949 17.8133 22.1193
Translational Efficiency 0.62 1.1401 0.7011 0.5861 0.8857 0.8895 0.7528 0.6441 0.5704 0.7594

Hit Data

Dataset Hit
Protein Concentration ✘
Protein Localization ✘
Gene Expression ✘
Translational Efficiency ✘

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
1444 1260 1214 1277 1409 1089 492 2219 2853 2349 1706 3496

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 907.84 1032.29 1349.44 1109.85 956.66 1125.03 1173.48 1078.45 931.95 1075.28 1298.69 1089.92
Standard Deviation 112.82 216.10 186.76 162.32 170.19 163.76 147.50 152.39 146.09 199.05 193.52 156.82
Intensity Change Log 2 — 0.185338 0.571851 0.289855 — 0.233885 0.294715 0.172881 — 0.210451 0.436306 0.231022

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.000158 0.000273 0.000273 0.001594 0.000256 0.000202 0.000108 0.000159 0.000206 0.000240 0.000225 0.000683
Bud Neck 0.002642 0.000851 0.000817 0.002075 0.001144 0.000788 0.002346 0.003200 0.001902 0.000822 0.001258 0.002789
Bud Site 0.012347 0.021015 0.006495 0.033903 0.018686 0.018034 0.006815 0.015645 0.015478 0.019633 0.006588 0.022314
Cell Periphery 0.000249 0.000124 0.000145 0.000126 0.000209 0.000090 0.000201 0.000044 0.000229 0.000108 0.000161 0.000074
Cytoplasm 0.678506* 0.831496* 0.813317* 0.720311* 0.746537* 0.810108* 0.502376* 0.683349* 0.712105* 0.821580* 0.723643* 0.696850*
Cytoplasmic Foci 0.200304* 0.079201 0.030552 0.061462 0.143836 0.111805 0.085665 0.029272 0.172416 0.094316 0.046446 0.041030
Eisosomes 0.000058 0.000007 0.000004 0.000012 0.000017 0.000007 0.000173 0.000014 0.000038 0.000007 0.000052 0.000013
Endoplasmic Reticulum 0.004735 0.001987 0.001505 0.000295 0.003212 0.000829 0.001149 0.000300 0.003983 0.001450 0.001402 0.000298
Endosome 0.013638 0.007928 0.004243 0.007561 0.013121 0.007185 0.010306 0.004869 0.013383 0.007583 0.005992 0.005852
Golgi 0.007213 0.005489 0.001799 0.005017 0.007612 0.010260 0.000240 0.001266 0.007410 0.007701 0.001349 0.002636
Lipid Particles 0.002597 0.000845 0.001523 0.000935 0.001432 0.000486 0.001626 0.000629 0.002021 0.000678 0.001553 0.000741
Mitochondria 0.000628 0.000374 0.000469 0.001065 0.001210 0.001145 0.000033 0.000422 0.000916 0.000731 0.000343 0.000656
Mitotic Spindle 0.001805 0.000612 0.001426 0.036890 0.003385 0.003865 0.000269 0.016784 0.002585 0.002120 0.001093 0.024128
None 0.001820 0.002375 0.001279 0.000492 0.000925 0.000502 0.000382 0.001247 0.001378 0.001507 0.001020 0.000971
Nuclear Periphery 0.000067 0.000072 0.000112 0.000145 0.000043 0.000010 0.000208 0.000141 0.000055 0.000043 0.000140 0.000142
Nuclear Periphery Foci 0.001651 0.000872 0.002964 0.001756 0.001028 0.000163 0.002998 0.000847 0.001343 0.000543 0.002974 0.001179
Nucleolus 0.000227 0.000558 0.000100 0.000235 0.000277 0.000065 0.000221 0.000278 0.000252 0.000329 0.000135 0.000262
Nucleus 0.009240 0.006923 0.014883 0.010143 0.007545 0.002584 0.066498 0.023811 0.008403 0.004911 0.029768 0.018819
Peroxisomes 0.000863 0.000833 0.000148 0.002374 0.000614 0.000802 0.000439 0.000953 0.000740 0.000819 0.000232 0.001472
Vacuole 0.057828 0.034102 0.116652 0.111020 0.044182 0.025938 0.317497* 0.215101* 0.051089 0.030317 0.174574 0.177083
Vacuole Periphery 0.003424 0.004064 0.001294 0.002590 0.004728 0.005133 0.000450 0.001668 0.004068 0.004560 0.001051 0.002005

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin -2.56 -2.69 -2.33 -1.96 -1.86 0.95 3.67 3.32 1.62 -0.10 -0.96 -1.05 -1.70 -1.39 -1.30
Bud Neck 9.42 8.87 3.44 -7.51 -6.75 3.90 -4.54 -5.99 -7.40 -1.29 10.07 4.62 -2.53 -9.27 -5.87
Bud Site -5.50 5.84 -4.87 -0.67 -7.65 0.32 7.42 5.05 3.96 -2.52 -3.30 9.74 0.28 3.21 -7.63
Cell Periphery 8.06 5.88 10.27 3.43 5.18 4.38 1.24 6.75 7.16 5.92 7.78 4.26 11.76 9.23 10.43
Cytoplasm -17.79 -13.33 2.45 17.64 13.96 -6.85 17.11 10.16 17.04 -11.05 -17.23 -0.85 8.57 25.16 7.24
Cytoplasmic Foci 21.61 35.60 30.28 7.17 -6.83 5.11 10.48 26.56 18.19 13.40 18.53 35.25 41.45 19.66 5.52
Eisosomes 15.25 16.50 13.57 -5.00 -9.96 3.61 -3.49 2.22 -2.31 3.68 14.00 -0.42 11.65 -4.26 3.08
Endoplasmic Reticulum 2.49 3.41 4.97 2.74 3.51 3.06 2.91 4.03 2.88 3.92 3.69 4.19 6.41 3.47 4.36
Endosome 5.41 12.52 10.43 3.44 -0.42 4.88 3.28 9.62 5.17 9.19 7.17 11.45 14.85 6.34 4.73
Golgi 3.22 13.64 5.92 3.56 -0.80 -2.25 18.16 15.86 8.48 -2.65 -0.52 19.29 15.76 9.97 0.05
Lipid Particles 7.20 3.68 6.70 -1.30 3.83 7.64 0.02 6.43 -0.84 5.29 9.65 2.64 9.51 -0.57 7.19
Mitochondria 1.06 0.45 0.28 -0.87 -0.29 0.07 1.70 1.36 1.54 -5.07 0.41 1.32 1.49 1.27 -0.19
Mitotic Spindle 1.34 0.31 -7.02 -7.61 -7.33 -0.26 2.33 -4.23 -3.91 -7.39 0.45 1.77 -8.24 -8.79 -10.05
None -0.72 1.07 3.29 2.47 1.06 2.09 2.86 -0.39 -2.85 -3.70 -0.30 1.09 2.14 1.58 0.15
Nuclear Periphery -0.12 -2.76 -7.52 -4.36 -5.68 5.15 -7.61 -12.80 -16.85 -0.79 0.59 -6.83 -13.53 -8.60 -6.16
Nuclear Periphery Foci 1.21 -2.36 -0.09 -4.58 5.20 5.34 -6.03 1.55 -2.95 6.99 2.38 -4.81 0.85 -3.68 9.03
Nucleolus -1.04 2.43 0.30 1.11 -2.16 1.94 0.73 0.21 -8.36 -1.97 -0.42 2.03 0.23 0.53 -4.56
Nucleus 2.60 -7.23 -10.31 -10.36 -2.59 5.27 -7.94 -14.03 -24.74 4.78 5.30 -9.72 -17.88 -23.38 1.73
Peroxisomes -0.15 6.28 -1.31 -1.15 -3.27 -2.12 3.00 0.11 1.07 -0.82 -0.95 8.02 -0.72 -0.11 -3.56
Vacuole 7.13 -8.70 -21.48 -24.95 -9.63 6.66 -23.24 -35.84 -40.19 5.79 9.52 -19.76 -41.19 -46.46 -7.56
Vacuole Periphery -1.02 5.48 4.29 4.77 -0.17 -0.38 6.72 5.57 4.59 -2.03 -0.80 8.04 7.23 6.70 -0.23
Standard name
Human Ortholog
Description AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner; directly phosphorylated by TORC1; phosphorylates ribosomal protein Rps6a/b (S6), in a TORC-dependent manner; undergoes autophosphorylation
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Ypk3

Ypk3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)
Molecular Function (Gene Ontology)
Cellular Component (Gene Ontology)

Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypk3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available