Standard name | |
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Description | AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner; directly phosphorylated by TORC1; phosphorylates ribosomal protein Rps6a/b (S6), in a TORC-dependent manner; undergoes autophosphorylation |
ensLOC | DeepLoc | |||||||||||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 | 3 | 3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 1 |
Bud | 2 | 0 | 5 | 2 | 1 | 1 | 6 | 9 | 6 | 9 | 8 | 4 | 7 | 1 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | – | – | – | – | – | – |
Cell Periphery | 5 | 3 | 4 | 4 | 4 | 10 | 7 | 7 | 8 | 14 | 3 | 5 | 15 | 10 | 19 | 7 | 2 | 5 | 0 | 0 | 0 | 0 | 0 | 0 |
Cytoplasm | 268 | 267 | 259 | 174 | 307 | 293 | 283 | 411 | 246 | 313 | 136 | 107 | 594 | 713 | 743 | 246 | 117 | 99 | 265 | 255 | 249 | 434 | 482 | 410 |
Endoplasmic Reticulum | 1 | 2 | 2 | 3 | 7 | 2 | 2 | 6 | 6 | 7 | 1 | 6 | 6 | 4 | 4 | 10 | 2 | 3 | 4 | 1 | 9 | 13 | 12 | 19 |
Endosome | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 1 | 1 | 0 | 0 | 1 | 0 | 0 | 2 | 1 | 1 | 1 | 0 | 0 | 1 | 2 | 2 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2 | 0 | 1 | 0 | 0 | 0 | 1 | 4 | 2 |
Mitochondria | 8 | 0 | 4 | 7 | 8 | 52 | 93 | 133 | 117 | 180 | 68 | 75 | 1 | 0 | 0 | 6 | 0 | 1 | 1 | 0 | 2 | 2 | 2 | 3 |
Nucleus | 0 | 1 | 8 | 6 | 1 | 1 | 2 | 4 | 2 | 0 | 0 | 0 | 3 | 2 | 4 | 3 | 2 | 1 | 0 | 2 | 0 | 1 | 1 | 0 |
Nuclear Periphery | 0 | 1 | 0 | 0 | 0 | 0 | 3 | 1 | 2 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 1 | 0 | 0 | 2 | 0 | 1 |
Nucleolus | 2 | 0 | 0 | 0 | 0 | 1 | 0 | 1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vac/Vac Membrane | 2 | 1 | 0 | 1 | 5 | 1 | 1 | 8 | 2 | 4 | 4 | 1 | 6 | 3 | 2 | 10 | 1 | 2 | 2 | 2 | 19 | 11 | 5 | 14 |
Unique Cell Count | 279 | 268 | 267 | 186 | 323 | 312 | 331 | 511 | 326 | 422 | 179 | 154 | 607 | 723 | 751 | 261 | 121 | 104 | 283 | 265 | 286 | 472 | 515 | 466 |
Labelled Cell Count | 289 | 275 | 282 | 197 | 333 | 361 | 398 | 583 | 393 | 531 | 220 | 198 | 633 | 733 | 773 | 286 | 125 | 113 | 283 | 265 | 286 | 472 | 515 | 466 |
Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Mean Cell GFP Intensity (1e-4) | 9.5 | 9.7 | 8.4 | 8.0 | 7.5 | 6.3 | 5.1 | 5.2 | 5.0 | 4.6 | 4.7 | 4.4 | 10.8 | 10.4 | 10.5 | 9.7 | 10.5 | 12.4 | 10.0 | 9.8 | 10.5 |
Std Deviation (1e-4) | 1.5 | 2.0 | 1.6 | 1.4 | 1.4 | 1.4 | 1.3 | 1.2 | 1.2 | 1.0 | 1.0 | 1.4 | 2.1 | 2.1 | 2.0 | 2.1 | 2.1 | 2.8 | 1.8 | 1.7 | 1.7 |
Intensity Change (Log2) | – | – | – | -0.07 | -0.15 | -0.42 | -0.72 | -0.69 | -0.74 | -0.87 | -0.85 | -0.93 | 0.36 | 0.31 | 0.32 | 0.21 | 0.33 | 0.57 | 0.26 | 0.22 | 0.32 |
Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
Cortical Patches | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Neck | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Bud Site | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Cell Periphery | 0 | 0 | 1.3 | 0 | 0 | 0.8 | 0 | 0 | 0 | 0.9 | 0 | 1.0 | 1.0 | 0 | 0 |
Cytoplasm | -1.8 | -1.2 | -1.8 | -4.8 | -6.3 | -7.3 | -7.8 | -6.8 | -8.1 | 0.8 | 1.7 | 2.2 | -1.5 | -0.2 | -0.9 |
Endoplasmic Reticulum | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 2.4 | 0 | 0 |
Endosome | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Golgi | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Mitochondria | 0 | 0.8 | 6.2 | 8.8 | 8.5 | 10.3 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleus | 0.1 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | -1.5 | 0 | 0 |
Nuclear Periphery | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Nucleolus | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Peroxisomes | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
SpindlePole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |
Vacuole | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 | 0 |