Standard name
Human Ortholog
Description AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner; directly phosphorylated by TORC1; phosphorylates ribosomal protein Rps6a/b (S6), in a TORC-dependent manner; undergoes autophosphorylation

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.96 1.0 0.97 0.94 0.95 0.94 0.85 0.8 0.75 0.74 0.76 0.69 0.98 0.99 0.99 0.94 0.97 0.95 0.94 0.96 0.87 0.92 0.94 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0.28 0.26 0.36 0.43 0.38 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 3 3 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 2 0 5 2 1 1 6 9 6 9 8 4 7 1 1 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 5 3 4 4 4 10 7 7 8 14 3 5 15 10 19 7 2 5 0 0 0 0 0 0
Cytoplasm 268 267 259 174 307 293 283 411 246 313 136 107 594 713 743 246 117 99 265 255 249 434 482 410
Endoplasmic Reticulum 1 2 2 3 7 2 2 6 6 7 1 6 6 4 4 10 2 3 4 1 9 13 12 19
Endosome 1 0 0 0 0 0 0 2 1 1 0 0 1 0 0 2 1 1 1 0 0 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 1 4 2
Mitochondria 8 0 4 7 8 52 93 133 117 180 68 75 1 0 0 6 0 1 1 0 2 2 2 3
Nucleus 0 1 8 6 1 1 2 4 2 0 0 0 3 2 4 3 2 1 0 2 0 1 1 0
Nuclear Periphery 0 1 0 0 0 0 3 1 2 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1
Nucleolus 2 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 1 0 1 5 1 1 8 2 4 4 1 6 3 2 10 1 2 2 2 19 11 5 14
Unique Cell Count 279 268 267 186 323 312 331 511 326 422 179 154 607 723 751 261 121 104 283 265 286 472 515 466
Labelled Cell Count 289 275 282 197 333 361 398 583 393 531 220 198 633 733 773 286 125 113 283 265 286 472 515 466


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 9.7 8.4 8.0 7.5 6.3 5.1 5.2 5.0 4.6 4.7 4.4 10.8 10.4 10.5 9.7 10.5 12.4 10.0 9.8 10.5
Std Deviation (1e-4) 1.5 2.0 1.6 1.4 1.4 1.4 1.3 1.2 1.2 1.0 1.0 1.4 2.1 2.1 2.0 2.1 2.1 2.8 1.8 1.7 1.7
Intensity Change (Log2) -0.07 -0.15 -0.42 -0.72 -0.69 -0.74 -0.87 -0.85 -0.93 0.36 0.31 0.32 0.21 0.33 0.57 0.26 0.22 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.3 0 0 0.8 0 0 0 0.9 0 1.0 1.0 0 0
Cytoplasm -1.8 -1.2 -1.8 -4.8 -6.3 -7.3 -7.8 -6.8 -8.1 0.8 1.7 2.2 -1.5 -0.2 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.8 6.2 8.8 8.5 10.3 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0 0 0 0 0 0 0 0 0 0 0 -1.5 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 8.7395 11.0763 10.1812 9.1608 8.9698 9.7568 3.4761 6.0233 5.481 4.8794 4.2004 5.3093 5.7927 8.7389 7.7979 6.9608 7.6942 7.7508
Actin 0.0131 0.0022 0.0094 0.0011 0.0166 0.0045 0.0257 0.0005 0.0077 0.0559 0.0114 0.0074 0.0139 0.0003 0.0081 0.017 0.0011 0.0023
Bud 0.0007 0.0006 0.0006 0.0008 0.0023 0.0004 0.0009 0.0002 0.0049 0.0021 0.0007 0.0007 0.0003 0.0001 0.0006 0.0007 0.0003 0.0002
Bud Neck 0.0054 0.0003 0.0025 0.0078 0.0014 0.0012 0.006 0.0002 0.0005 0.0006 0.0006 0.0031 0.0011 0.0001 0.0006 0.0036 0.0276 0.0012
Bud Periphery 0.0009 0.0005 0.0011 0.0003 0.0029 0.0003 0.0009 0.0001 0.0014 0.0012 0.0006 0.0005 0.0002 0 0.0004 0.0008 0.0004 0.0003
Bud Site 0.0033 0.0036 0.0022 0.0081 0.0229 0.0002 0.0044 0.0011 0.0144 0.0022 0.0012 0.0003 0.0029 0.0013 0.0133 0.0074 0.0003 0.0003
Cell Periphery 0.0008 0.0006 0.001 0.0003 0.0004 0.0003 0.0003 0.0003 0.0005 0.0003 0.0001 0.0001 0.0003 0.0005 0.0005 0.0007 0.0003 0.0003
Cytoplasm 0.7455 0.9725 0.8783 0.9033 0.7483 0.9435 0.6687 0.9424 0.8626 0.8028 0.7677 0.893 0.819 0.9846 0.9219 0.8233 0.9043 0.9633
Cytoplasmic Foci 0.0313 0.0031 0.0166 0.0198 0.0142 0.0063 0.0575 0.0064 0.0277 0.0171 0.0535 0.0165 0.042 0.0039 0.0128 0.0269 0.0073 0.005
Eisosomes 0.0009 0.0001 0.0001 0 0.0002 0 0.0003 0.0002 0.0001 0.0004 0.0001 0 0.0001 0 0 0.0015 0 0
Endoplasmic Reticulum 0.0576 0.0034 0.0095 0.0059 0.0035 0.0156 0.0165 0.0038 0.0062 0.0021 0.0044 0.0142 0.013 0.0022 0.0065 0.0026 0.0019 0.0119
Endosome 0.0331 0.0029 0.0239 0.0211 0.1006 0.0088 0.0675 0.0043 0.0258 0.0395 0.0293 0.0314 0.0317 0.0017 0.013 0.0281 0.0106 0.0068
Golgi 0.01 0.0006 0.0098 0.0023 0.017 0.0032 0.0306 0.0005 0.0081 0.0137 0.0288 0.0176 0.0129 0.0003 0.0043 0.0125 0.0077 0.0018
Lipid Particles 0.0048 0.0001 0.0045 0.0024 0.0037 0.0033 0.0305 0.0001 0.0077 0.0108 0.0245 0.0012 0.0168 0.0001 0.001 0.0076 0.0147 0.0001
Mitochondria 0.0263 0.0004 0.0055 0.001 0.0237 0.0048 0.0122 0.0001 0.0087 0.0365 0.0513 0.0072 0.0045 0 0.002 0.0289 0.0144 0.0036
None 0.0172 0.0041 0.0098 0.0018 0.0009 0.0022 0.0254 0.0368 0.0113 0.0014 0.0045 0.0023 0.0173 0.0029 0.008 0.0033 0.0022 0.0008
Nuclear Periphery 0.0079 0.0005 0.0062 0.0013 0.0039 0.0007 0.0025 0.0003 0.0017 0.0014 0.0006 0.0009 0.0019 0.0003 0.0007 0.0007 0.0004 0.0004
Nucleolus 0.0011 0 0.0006 0.0009 0.0005 0 0.0009 0.0001 0.0003 0.0002 0.0001 0 0.0004 0 0.0002 0.0007 0.0001 0
Nucleus 0.0215 0.001 0.0042 0.0022 0.0017 0.0009 0.002 0.0004 0.0011 0.0008 0.0006 0.0005 0.0011 0.0004 0.0008 0.0012 0.0006 0.0005
Peroxisomes 0.004 0 0.0021 0.0023 0.003 0.001 0.0311 0 0.0057 0.0011 0.017 0.0008 0.0104 0 0.0012 0.025 0.0023 0.0001
Punctate Nuclear 0.0035 0.0001 0.0013 0.001 0.0006 0.0001 0.0072 0.0001 0.0008 0.0002 0.0007 0.0003 0.0057 0.0001 0.0003 0.0004 0.0001 0
Vacuole 0.0094 0.0027 0.0092 0.0145 0.023 0.002 0.0072 0.0021 0.0024 0.0072 0.0016 0.0014 0.0039 0.0009 0.0033 0.006 0.0027 0.0008
Vacuole Periphery 0.0017 0.0004 0.0015 0.0017 0.0087 0.0006 0.002 0.0001 0.0006 0.0027 0.0007 0.0006 0.0006 0.0001 0.0003 0.0011 0.0007 0.0002

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 26.9208 16.2521 14.8428 15.9251 18.132 18.1702 23.8587 22.3949 17.8133 22.1193
Translational Efficiency 0.62 1.1401 0.7011 0.5861 0.8857 0.8895 0.7528 0.6441 0.5704 0.7594

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency
Standard name
Human Ortholog
Description AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner; directly phosphorylated by TORC1; phosphorylates ribosomal protein Rps6a/b (S6), in a TORC-dependent manner; undergoes autophosphorylation
Localization
Cell Percentages cytoplasm (100%)
Cell Cycle Regulation No
Subcompartmental Group cytoplasm-4

Ypk3

Ypk3


Nearest neighbours ( genes)

correlation threshold

0.6

1.0

0.80


Biological Process (Gene Ontology)

Molecular Function (Gene Ontology)

Cellular Component (Gene Ontology)


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Ypk3-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available