Standard name
Description AGC kinase; phosphorylated by cAMP-dependent protein kinase (PKA) in a TORC1-dependent manner; directly phosphorylated by TORC1; phosphorylates ribosomal protein Rps6a/b (S6), in a TORC-dependent manner; undergoes autophosphorylation
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Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0 0 0 0 0
Cytoplasm 0.96 1.0 0.97 0.94 0.95 0.94 0.85 0.8 0.75 0.74 0.76 0.69 0.98 0.99 0.99 0.94 0.97 0.95 0.94 0.96 0.87 0.92 0.94 0.88
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.17 0.28 0.26 0.36 0.43 0.38 0.49 0 0 0 0 0 0 0 0 0 0 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.07 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 1 1 3 3 0 0 0 0 0 0 0 0 0 0 0 0 1 1
Bud 2 0 5 2 1 1 6 9 6 9 8 4 7 1 1 0 0 0 0 0 0 0 0 1
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 – – – – – –
Cell Periphery 5 3 4 4 4 10 7 7 8 14 3 5 15 10 19 7 2 5 0 0 0 0 0 0
Cytoplasm 268 267 259 174 307 293 283 411 246 313 136 107 594 713 743 246 117 99 265 255 249 434 482 410
Endoplasmic Reticulum 1 2 2 3 7 2 2 6 6 7 1 6 6 4 4 10 2 3 4 1 9 13 12 19
Endosome 1 0 0 0 0 0 0 2 1 1 0 0 1 0 0 2 1 1 1 0 0 1 2 2
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 2 0 1 0 0 0 1 4 2
Mitochondria 8 0 4 7 8 52 93 133 117 180 68 75 1 0 0 6 0 1 1 0 2 2 2 3
Nucleus 0 1 8 6 1 1 2 4 2 0 0 0 3 2 4 3 2 1 0 2 0 1 1 0
Nuclear Periphery 0 1 0 0 0 0 3 1 2 0 0 0 0 0 0 0 0 0 1 0 0 2 0 1
Nucleolus 2 0 0 0 0 1 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 2 1 0 1 5 1 1 8 2 4 4 1 6 3 2 10 1 2 2 2 19 11 5 14
Unique Cell Count 279 268 267 186 323 312 331 511 326 422 179 154 607 723 751 261 121 104 283 265 286 472 515 466
Labelled Cell Count 289 275 282 197 333 361 398 583 393 531 220 198 633 733 773 286 125 113 283 265 286 472 515 466


Cytoplasm

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 9.5 9.7 8.4 8.0 7.5 6.3 5.1 5.2 5.0 4.6 4.7 4.4 10.8 10.4 10.5 9.7 10.5 12.4 10.0 9.8 10.5
Std Deviation (1e-4) 1.5 2.0 1.6 1.4 1.4 1.4 1.3 1.2 1.2 1.0 1.0 1.4 2.1 2.1 2.0 2.1 2.1 2.8 1.8 1.7 1.7
Intensity Change (Log2) – – – -0.07 -0.15 -0.42 -0.72 -0.69 -0.74 -0.87 -0.85 -0.93 0.36 0.31 0.32 0.21 0.33 0.57 0.26 0.22 0.32


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 1.3 0 0 0.8 0 0 0 0.9 0 1.0 1.0 0 0
Cytoplasm -1.8 -1.2 -1.8 -4.8 -6.3 -7.3 -7.8 -6.8 -8.1 0.8 1.7 2.2 -1.5 -0.2 -0.9
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 2.4 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0.8 6.2 8.8 8.5 10.3 0 0 0 0 0 0 0 0 0
Nucleus 0.1 0 0 0 0 0 0 0 0 0 0 0 -1.5 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0