Standard name
Description Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.24
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0.91 0.91 0.92 0.94 0.83 0.73 0.69 0.52 0.5 0.52 0.55 0.62 0.7 0.52 0.68 0.48 0.28
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0.12 0 0 0 0.09 0.08 0
Endosome 0 0 0 0 0 0 0 0 0 0 0.13 0.05 0.05 0.06 0 0.06 0.06
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.14 0.15
Mitochondria 0.1 0 0.13 0.11 0.37 0.53 0.49 0.67 0.66 0.73 0.08 0.18 0.12 0.2 0.16 0 0
Nucleus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0.23 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 1 0 0 0 0 0 0 0 0 2 0 6 7 0 0 0
Bud 2 5 2 2 2 5 8 6 3 8 2 0 3 3 0 0 0
Bud Neck 3 5 2 0 3 0 4 3 2 1 3 0 3 2 0 0 0
Bud Site 0 0 0 0 1 1 2 2 0 3 0
Cell Periphery 0 1 0 0 0 0 0 1 3 0 1 0 1 2 0 0 0
Cytoplasm 335 214 122 175 205 247 281 108 120 171 90 36 270 126 4 2 1
Endoplasmic Reticulum 6 0 1 0 0 0 3 0 0 1 19 0 1 6 0 0 0
Endosome 2 3 0 2 0 1 1 0 0 0 21 2 18 14 0 0 0
Golgi 0 2 0 0 0 0 0 0 0 0 0 1 2 2 0 0 0
Mitochondria 35 10 17 21 92 180 202 139 159 242 13 10 46 49 0 0 0
Nucleus 0 0 0 1 1 0 1 0 1 0 3 0 0 0 0 0 0
Nuclear Periphery 0 0 1 0 0 0 1 0 1 1 1 0 0 0 0 0 0
Nucleolus 0 0 0 0 1 0 0 0 0 0 3 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 1 0 0 0 3 1 3 4 0 0 0
SpindlePole 2 6 2 0 3 4 7 5 2 9 38 1 7 6 0 0 0
Vac/Vac Membrane 2 4 0 1 1 2 2 4 4 2 4 1 10 7 0 0 0
Unique Cell Count 367 234 133 186 248 338 409 209 242 331 163 59 385 242 6 5 4
Labelled Cell Count 387 251 147 202 309 440 513 268 295 438 203 59 385 242 6 5 4


Lipid Particles

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 3.7 3.6 3.1 3.2 2.6 2.4 2.8 2.4 2.5 2.5 4.3 4.4 4.9
Std Deviation (1e-4) 0.6 1.3 0.7 1.2 1.2 0.6 0.7 0.4 0.8 0.5 1.2 0.9 1.5
Intensity Change (Log2) -0.24 -0.19 -0.46 -0.62 -0.39 -0.62 -0.56 -0.56 0.24 0.29 0.44


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Actin
Bud
Bud Neck
Bud Site
Cell Periphery
Cyto
Endoplasmic Reticulum
Endosome
Golgi
Mitochondria
Nuclear Periphery
Nuc
Nucleolus
Peroxisomes
SpindlePole
Vac
Cortical Patches
Cytoplasm
Nucleus
Vacuole