| Standard name | |
|---|---|
| Description | Very long chain fatty acyl-CoA synthetase and fatty acid transporter; activates imported fatty acids with a preference for very long lengths (C20-C26); has a separate function in the transport of long chain fatty acids |
Micrographs
Sub-cellular Localization
Yeast GFP Assignment
Protein Abundance
Localization Change
External localization resources
| ensLOC | DeepLoc | |||||||||||||||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Localization | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | WT1 | WT2 | WT3 | AF100 | AF140 | AF180 |
| Cortical Patches | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | 2 | 0 | 6 | 7 | 0 | 0 | 0 |
| Bud | – | 2 | 5 | 2 | 2 | 2 | 5 | 8 | – | 6 | 3 | 8 | – | – | – | – | – | 2 | 0 | 3 | 3 | 0 | 0 | 0 |
| Bud Neck | – | 3 | 5 | 2 | 0 | 3 | 0 | 4 | – | 3 | 2 | 1 | – | – | – | – | – | 3 | 0 | 3 | 2 | 0 | 0 | 0 |
| Bud Site | – | 0 | 0 | 0 | 0 | 1 | 1 | 2 | – | 2 | 0 | 3 | – | – | – | – | – | 0 | – | – | – | – | – | – |
| Cell Periphery | – | 0 | 1 | 0 | 0 | 0 | 0 | 0 | – | 1 | 3 | 0 | – | – | – | – | – | 1 | 0 | 1 | 2 | 0 | 0 | 0 |
| Cytoplasm | – | 335 | 214 | 122 | 175 | 205 | 247 | 281 | – | 108 | 120 | 171 | – | – | – | – | – | 90 | 36 | 270 | 126 | 4 | 2 | 1 |
| Endoplasmic Reticulum | – | 6 | 0 | 1 | 0 | 0 | 0 | 3 | – | 0 | 0 | 1 | – | – | – | – | – | 19 | 0 | 1 | 6 | 0 | 0 | 0 |
| Endosome | – | 2 | 3 | 0 | 2 | 0 | 1 | 1 | – | 0 | 0 | 0 | – | – | – | – | – | 21 | 2 | 18 | 14 | 0 | 0 | 0 |
| Golgi | – | 0 | 2 | 0 | 0 | 0 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | 0 | 1 | 2 | 2 | 0 | 0 | 0 |
| Mitochondria | – | 35 | 10 | 17 | 21 | 92 | 180 | 202 | – | 139 | 159 | 242 | – | – | – | – | – | 13 | 10 | 46 | 49 | 0 | 0 | 0 |
| Nucleus | – | 0 | 0 | 0 | 1 | 1 | 0 | 1 | – | 0 | 1 | 0 | – | – | – | – | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nuclear Periphery | – | 0 | 0 | 1 | 0 | 0 | 0 | 1 | – | 0 | 1 | 1 | – | – | – | – | – | 1 | 0 | 0 | 0 | 0 | 0 | 0 |
| Nucleolus | – | 0 | 0 | 0 | 0 | 1 | 0 | 0 | – | 0 | 0 | 0 | – | – | – | – | – | 3 | 0 | 0 | 0 | 0 | 0 | 0 |
| Peroxisomes | – | 0 | 0 | 0 | 0 | 0 | 0 | 1 | – | 0 | 0 | 0 | – | – | – | – | – | 3 | 1 | 3 | 4 | 0 | 0 | 0 |
| SpindlePole | – | 2 | 6 | 2 | 0 | 3 | 4 | 7 | – | 5 | 2 | 9 | – | – | – | – | – | 38 | 1 | 7 | 6 | 0 | 0 | 0 |
| Vac/Vac Membrane | – | 2 | 4 | 0 | 1 | 1 | 2 | 2 | – | 4 | 4 | 2 | – | – | – | – | – | 4 | 1 | 10 | 7 | 0 | 0 | 0 |
| Unique Cell Count | 367 | 234 | 133 | 186 | 248 | 338 | 409 | 209 | 242 | 331 | 163 | 59 | 385 | 242 | 6 | 5 | 4 | |||||||
| Labelled Cell Count | 387 | 251 | 147 | 202 | 309 | 440 | 513 | 268 | 295 | 438 | 203 | 59 | 385 | 242 | 6 | 5 | 4 | |||||||
Yeast GFP Assignment
Protein Abundance
| Screen | WT1 | WT2 | WT3 | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 | AF100 | AF140 | AF180 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Mean Cell GFP Intensity (1e-4) | – | 3.7 | 3.6 | 3.1 | 3.2 | 2.6 | 2.4 | 2.8 | – | 2.4 | 2.5 | 2.5 | – | – | – | 4.3 | 4.4 | 4.9 | – | – | – |
| Std Deviation (1e-4) | – | 0.6 | 1.3 | 0.7 | 1.2 | 1.2 | 0.6 | 0.7 | – | 0.4 | 0.8 | 0.5 | – | – | – | 1.2 | 0.9 | 1.5 | – | – | – |
| Intensity Change (Log2) | – | – | – | -0.24 | -0.19 | -0.46 | -0.62 | -0.39 | – | -0.62 | -0.56 | -0.56 | – | – | – | 0.24 | 0.29 | 0.44 | – | – | – |
Localization Change
| Localization | RAP60 | RAP140 | RAP220 | RAP300 | RAP380 | RAP460 | RAP540 | RAP620 | RAP700 | HU80 | HU120 | HU160 | rpd3Δ_1 | rpd3Δ_2 | rpd3Δ_3 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Actin | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Neck | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Bud Site | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cell Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cyto | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endoplasmic Reticulum | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Endosome | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Golgi | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Mitochondria | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuclear Periphery | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nuc | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleolus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Peroxisomes | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| SpindlePole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vac | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cortical Patches | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Cytoplasm | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Nucleus | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
| Vacuole | – | – | – | – | – | – | – | – | – | – | – | – | – | – | – |
External localization resources
