Standard name
Human Ortholog
Description Multidrug transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; has a role in polyamine homeostasis; involved in spore wall asembly; sequence similarity to DTR1 and QDR1, and the triple mutant dtr1 qdr1 qdr3 exhibits reduced dityrosine fluorescence relative to the single mutants; expression is upregulated under polyamine stress; required for resistance to quinidine, barban, cisplatin, and bleomycin

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0.13 0.34 0.18 0.19 0.14 0.3 0.19 0.15 0.12 0.1 0.13 0.07 0.08 0.09 0.05 0 0 0 0 0.05 0
Bud 0 0 0 0 0 0 0 0.05 0 0 0.05 0.05 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.06 0.06
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0.94 0.95 0.88 0.85 0.83 0.63 0.48 0.4 0.36 0.37 0.36 0.42 0.86 0.79 0.83 0.97 0.9 0.91 0.88 0.84 0.82
Cytoplasm 0 0.13 0.09 0.1 0.09 0.14 0.23 0.31 0.37 0.44 0.36 0.46 0.18 0.18 0.17 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0.12 0.29 0.34 0.24 0.27 0.19 0.22 0.15 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 0 0 0 0 0.11 0.15 0 0 0 0 0 0.05
Nucleus 0 0 0 0 0 0 0 0 0 0.06 0.06 0.06 0 0 0 0 0 0 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.08 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0.05 0.13 0.1 0.13 0.13 0.12 0 0 0 0 0 0 0 0 0
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 27 64 40 31 35 88 76 61 31 30 41 24 21 14 10 0 4 4 0 1 0
Bud 3 5 0 1 3 9 11 20 8 13 17 17 2 2 3 1 1 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 0 0 1 0
Bud Site 0 0 0 0 0 0 1 0 0 0 0 0 0 0 0
Cell Periphery 193 181 197 139 206 184 194 165 93 111 112 143 213 128 162 224 202 239 7 17 8
Cytoplasm 8 24 21 17 23 42 93 129 97 131 112 156 46 30 34 2 5 2 0 0 0
Endoplasmic Reticulum 4 2 4 3 29 84 137 100 71 56 69 53 6 2 5 0 2 6 0 0 0
Endosome 0 0 0 0 0 1 0 0 0 0 1 0 2 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 1 2 0 0 0 3 0 0 1 0 0 0 0 0 0
Mitochondria 0 1 3 1 0 2 3 4 1 3 8 3 8 18 29 1 4 5 0 0 0
Nucleus 0 0 1 0 0 2 13 15 8 19 18 19 1 1 2 0 1 0 0 0 0
Nuclear Periphery 0 0 0 1 1 1 1 1 1 2 1 2 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 1 0 0 1 3 4 2 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 2 1 0 0 0 0 0 0 1 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 1 5 3 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 1 0 1 0 0 5 22 52 27 39 42 41 0 1 1 0 0 1 0 0 0
Unique Cell Count 206 191 225 163 247 292 407 412 259 300 312 342 249 163 196 231 226 263 9 21 11
Labelled Cell Count 236 277 267 193 297 422 558 552 338 407 425 463 299 197 247 231 226 263 9 21 11


Cell Periphery

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 34.0 26.5 30.7 31.1 28.1 22.4 21.4 20.5 20.5 19.4 20.0 21.0 16.6 18.6 24.1
Std Deviation (1e-4) 7.2 6.2 7.9 7.8 7.7 8.1 7.5 6.5 6.6 6.2 6.3 5.9 4.6 5.4 6.4
Intensity Change (Log2) 0.02 -0.13 -0.45 -0.52 -0.58 -0.58 -0.66 -0.62 -0.55 -0.88 -0.72 -0.35


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0.3 -1.1 3.2 0.3 -1.0 -1.8 -2.6 -1.5 -4.0 -3.0 -2.6 -4.0
Bud 0 0 0 0 3.4 0 3.2 3.6 3.4 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery -0.7 -1.3 -6.3 -9.9 -11.6 -11.6 -11.6 -11.9 -10.9 -0.6 -2.4 -1.4
Cytoplasm 0.4 0 1.7 4.2 6.2 7.2 8.6 7.0 9.1 2.9 2.6 2.4
Endoplasmic Reticulum 0 4.2 8.1 9.2 7.3 7.8 6.0 6.8 5.3 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0 0 0 0 1.4 4.2 5.2
Nucleus 0 0 0 0 2.5 0 3.5 3.3 3.2 0 0 0
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0 0 0 0 0 0 0 0 0 0 0 0
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 3.2 5.3 4.7 5.4 5.5 5.1 0 0 0

Images

Circle plot not available

Protein Concentration and Protein Localization Data

R1 R2 R3
G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Pre-START G1 Post-START S/G2 Metaphase Anaphase Telophase
Concentration 2.9922 4.3912 3.6005 3.6327 2.992 4.0719 2.5409 3.4639 3.4565 3.2287 2.321 4.1186 3.4751 4.8007 3.8987 3.3311 3.0846 4.0224
Actin 0.0314 0.0011 0.0256 0.0551 0.0687 0.0092 0.006 0.0119 0.0005 0.0004 0.0286 0.0026 0.0612 0.0062 0.0247 0.0016 0.0305 0.0069
Bud 0.0024 0.0002 0.001 0.0009 0.0046 0.0009 0.0003 0.001 0.0003 0.0003 0.0013 0.0004 0.0013 0.0002 0.0004 0.0004 0.0024 0.0014
Bud Neck 0.01 0.0023 0.0325 0.0081 0.0113 0.0263 0.0008 0.0023 0.0149 0.0139 0.0109 0.0475 0.007 0.0027 0.0121 0.037 0.0153 0.0106
Bud Periphery 0.0134 0.001 0.0067 0.0111 0.0163 0.011 0.0006 0.0055 0.0032 0.0041 0.0184 0.0068 0.0085 0.0018 0.0022 0.0066 0.0162 0.0057
Bud Site 0.0272 0.0614 0.0461 0.005 0.0093 0.0023 0.0052 0.034 0.0243 0.0026 0.0088 0.0013 0.0199 0.0799 0.0457 0.0132 0.0178 0.0016
Cell Periphery 0.5565 0.5337 0.5615 0.5733 0.4736 0.6736 0.2798 0.5001 0.6564 0.7399 0.4818 0.7309 0.5928 0.5886 0.6938 0.6815 0.5967 0.7814
Cytoplasm 0.2239 0.3647 0.2699 0.2738 0.2002 0.2235 0.5069 0.4245 0.2758 0.2315 0.2337 0.168 0.2253 0.2874 0.1891 0.2509 0.2164 0.1662
Cytoplasmic Foci 0.0102 0.001 0.0045 0.0021 0.0142 0.0014 0.0155 0.0007 0.0004 0.0004 0.0362 0.0003 0.0048 0.0051 0.0032 0.0003 0.0063 0.0071
Eisosomes 0.0063 0.0018 0.0059 0.0013 0.0219 0.0026 0.002 0.0028 0.0009 0.0002 0.0071 0.0004 0.021 0.0017 0.0043 0.0007 0.0034 0.0003
Endoplasmic Reticulum 0.0112 0.0094 0.0047 0.0097 0.0143 0.0063 0.0327 0.006 0.0025 0.0019 0.0068 0.002 0.0077 0.0032 0.0068 0.0031 0.0066 0.0026
Endosome 0.014 0.001 0.0049 0.0017 0.0259 0.0014 0.055 0.0003 0 0 0.0376 0.0005 0.0051 0.0032 0.0011 0 0.021 0.0006
Golgi 0.0052 0.0003 0.0045 0.0027 0.0166 0.0015 0.0118 0.0009 0.0001 0.0001 0.0479 0.0009 0.0067 0.0034 0.003 0.0002 0.0089 0.0011
Lipid Particles 0.0066 0.0027 0.0036 0.0097 0.0235 0.0044 0.0083 0.0041 0.0006 0.0004 0.0516 0.002 0.0056 0.0056 0.0021 0.0005 0.0056 0.0017
Mitochondria 0.0044 0 0.0037 0.0079 0.0131 0.0029 0.0058 0.0001 0 0 0.0113 0.027 0.0032 0.0006 0.001 0 0.0085 0.0002
None 0.0386 0.0069 0.0054 0.0038 0.0383 0.0129 0.0399 0.001 0.0167 0.0013 0.0031 0.0018 0.009 0.0024 0.0015 0.0011 0.0039 0.0024
Nuclear Periphery 0.0128 0.0005 0.0031 0.0004 0.0114 0.003 0.0055 0.0004 0.0002 0.0001 0.0004 0.0001 0.0085 0.0003 0.0002 0.0001 0.0117 0.0001
Nucleolus 0.0006 0.0001 0.0014 0.0005 0.0009 0.0009 0.0002 0.0002 0.0001 0 0.0001 0.0001 0.0004 0.0001 0.0001 0.0001 0.0011 0.0001
Nucleus 0.0085 0.0003 0.0047 0.0005 0.0038 0.0013 0.0022 0.0002 0.0002 0.0002 0.0002 0.0002 0.0017 0.0002 0.0002 0.0002 0.0053 0.0002
Peroxisomes 0.0037 0 0.0017 0.0045 0.0072 0.0008 0.0006 0.0001 0 0 0.0076 0.0007 0.0037 0.0021 0.0055 0 0.0014 0.0012
Punctate Nuclear 0.0019 0.0001 0.0021 0.0003 0.0093 0.0028 0.0031 0.0001 0 0 0.0004 0 0.0014 0.0001 0.0002 0 0.0116 0.0013
Vacuole 0.0097 0.0114 0.006 0.0274 0.0141 0.0109 0.017 0.0039 0.003 0.0027 0.0058 0.0064 0.0046 0.0053 0.0027 0.0024 0.0081 0.0072
Vacuole Periphery 0.0013 0.0001 0.0004 0.0003 0.0016 0.0002 0.001 0.0001 0 0 0.0005 0.0002 0.0006 0 0 0 0.0013 0

Sequencing Data

R1 R2
G1 Post-START S/G2 Metaphase Anaphase Telophase G1 Post-START S/G2 Metaphase Anaphase Telophase
Gene Expression 11.9935 25.255 21.2155 21.239 17.6235 20.7773 29.2693 27.803 27.1588 26.4123
Translational Efficiency 0.3444 0.3361 0.3271 0.2952 0.3049 0.2796 0.3045 0.2507 0.2331 0.2306

Hit Data

Dataset Hit
Protein Concentration
Protein Localization
Gene Expression
Translational Efficiency

Micrographs

Cell Count

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
158 769 160 707 1737 1250 923 174 1895 2019 1083 881

Protein Abundance

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Mean 997.32 1858.52 1297.13 1096.46 2132.64 1847.18 1341.69 966.20 2037.98 1851.50 1335.11 1070.73
Standard Deviation 150.15 469.24 183.74 181.79 505.35 376.04 250.23 104.16 578.34 414.05 242.08 177.07
Intensity Change Log 2 0.898026 0.379195 0.136725 -0.207316 -0.668589 -1.142247 0.243602 -0.246251 -0.601638

Localization Score (DeepLoc)

R1 R2 R1 & R2
WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14 WT UBP2 UBP14 UBP2UBP14
Actin 0.006743 0.002253 0.031677 0.048170 0.000431 0.000618 0.007719 0.044390 0.000957 0.001241 0.011258 0.047424
Bud Neck 0.027441 0.003963 0.037494 0.077703 0.000597 0.000646 0.024800 0.047693 0.002835 0.001910 0.026675 0.071776
Bud Site 0.041278 0.047374 0.153142* 0.274644* 0.008320 0.009850 0.077003 0.137664* 0.011068 0.024142 0.088252 0.247590*
Cell Periphery 0.060025 0.899162* 0.384518* 0.235349* 0.952340* 0.970903* 0.819939* 0.179475* 0.877941* 0.943578* 0.755611* 0.224314*
Cytoplasm 0.252144* 0.029306 0.108687* 0.185051* 0.019229 0.002296 0.043157 0.323392* 0.038649 0.012583 0.052838 0.212374*
Cytoplasmic Foci 0.036806 0.000957 0.020380 0.010239 0.000280 0.001454 0.000461 0.042764 0.003326 0.001265 0.003403 0.016663
Eisosomes 0.014887 0.001299 0.004734 0.013823 0.000599 0.000593 0.004800 0.032827 0.001790 0.000862 0.004791 0.017576
Endoplasmic Reticulum 0.017282 0.002648 0.035253 0.013977 0.001637 0.002638 0.005954 0.012057 0.002941 0.002642 0.010283 0.013598
Endosome 0.009960 0.000719 0.011659 0.001625 0.000398 0.000774 0.000378 0.005056 0.001195 0.000753 0.002045 0.002303
Golgi 0.009462 0.001449 0.003863 0.001069 0.000977 0.001374 0.000509 0.005970 0.001684 0.001403 0.001004 0.002037
Lipid Particles 0.002883 0.001858 0.021976 0.003445 0.000584 0.000215 0.001380 0.006125 0.000776 0.000840 0.004423 0.003974
Mitochondria 0.004232 0.001230 0.000049 0.000121 0.000308 0.000486 0.000266 0.000100 0.000635 0.000770 0.000234 0.000117
Mitotic Spindle 0.002602 0.000343 0.000410 0.000146 0.000283 0.000477 0.000399 0.000764 0.000477 0.000426 0.000400 0.000268
None 0.000164 0.000069 0.000017 0.000054 0.000061 0.000015 0.000031 0.000055 0.000070 0.000035 0.000029 0.000054
Nuclear Periphery 0.000283 0.000197 0.000245 0.000082 0.000561 0.000660 0.000241 0.000085 0.000537 0.000484 0.000242 0.000083
Nuclear Periphery Foci 0.002152 0.000347 0.006992 0.002722 0.000221 0.000123 0.000490 0.003463 0.000382 0.000208 0.001451 0.002869
Nucleolus 0.000055 0.000031 0.000025 0.000023 0.000136 0.000028 0.000022 0.000055 0.000129 0.000029 0.000022 0.000029
Nucleus 0.044953 0.000227 0.003870 0.012089 0.000722 0.000063 0.001313 0.025506 0.004410 0.000125 0.001691 0.014739
Peroxisomes 0.000741 0.000113 0.001229 0.000545 0.000058 0.000100 0.000180 0.001541 0.000115 0.000105 0.000335 0.000742
Vacuole 0.464781* 0.005047 0.173219 0.119025 0.009227 0.005564 0.010641 0.130886 0.047210 0.005367 0.034660 0.121368
Vacuole Periphery 0.001128 0.001410 0.000559 0.000097 0.003032 0.001122 0.000317 0.000130 0.002873 0.001232 0.000353 0.000103

Localization Changes (T-score)

R1 R2 R1 & R2
UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14 UBP2_WT UBP14_WT UBP2UBP14_WT UBP2UBP14_UBP2 UBP2UBP14_UBP14
Actin 2.49 -3.99 -12.73 -14.84 -2.31 -1.09 -6.58 -7.06 -7.03 -5.77 -1.52 -7.65 -16.95 -16.79 -11.75
Bud Neck 2.84 -1.61 -6.22 -14.82 -5.39 -0.43 -10.89 -7.32 -7.31 -3.28 0.94 -11.33 -16.43 -16.72 -9.51
Bud Site -1.21 -6.32 -19.58 -19.51 -5.74 -0.73 -12.86 -10.09 -9.92 -4.27 -5.34 -14.57 -26.21 -24.17 -15.13
Cell Periphery -60.86* -10.54 -11.82 52.10* 4.81 -3.97 14.31 48.73* 50.00* 36.09* -8.78 10.48 60.62 77.01* 41.02*
Cytoplasm 9.78 5.31 2.52 -18.56 -5.10 7.40 -5.58 -19.11 -20.35 -17.29 7.35 -3.18 -23.06 -28.06 -20.17
Cytoplasmic Foci 7.82 2.15 5.46 -8.25 1.74 -2.06 -1.86 -8.66 -8.38 -8.63 3.57 -0.19 -9.37 -10.90 -8.28
Eisosomes 2.79 1.90 -0.32 -11.37 -6.90 0.05 -7.03 -9.10 -9.10 -7.78 1.97 -5.18 -13.42 -14.64 -10.14
Endoplasmic Reticulum 2.40 -2.04 0.20 -6.83 2.50 -1.13 -4.42 -5.95 -5.00 -3.08 0.19 -4.77 -7.62 -7.80 -1.73
Endosome 4.04 -0.70 3.59 -3.47 4.11 -1.63 0.23 -7.93 -6.89 -8.01 1.58 -2.09 -4.17 -6.21 -0.71
Golgi 1.50 0.93 1.60 1.44 4.15 -0.78 2.56 -7.97 -5.93 -9.06 0.28 1.37 -1.08 -1.69 -5.15
Lipid Particles 1.51 -3.73 -1.22 -2.95 3.61 2.37 -1.42 -9.48 -10.30 -5.92 -0.28 -3.96 -11.48 -10.05 0.42
Mitochondria 0.92 1.44 1.42 1.74 -5.25 -0.88 0.70 3.64 2.03 5.33 -0.57 1.65 2.24 2.42 4.48
Mitotic Spindle 2.24 2.14 2.44 7.40 4.39 -3.45 -4.48 -0.74 -0.43 -0.55 0.53 0.79 1.41 1.27 1.13
None 1.39 2.06 1.65 0.63 -3.45 3.22 2.08 0.35 -4.05 -2.40 2.09 2.83 1.12 -1.59 -3.31
Nuclear Periphery 1.97 1.20 3.57 8.12 5.50 -1.12 3.92 5.92 14.25 9.07 0.75 4.01 6.22 15.36 10.64
Nuclear Periphery Foci 6.13 -4.06 -1.60 -10.90 3.60 3.03 -4.90 -9.72 -10.06 -8.87 4.16 -5.60 -13.13 -14.28 -5.69
Nucleolus 2.84 2.72 3.43 1.90 0.62 1.54 1.62 1.15 -5.13 -6.33 1.57 1.66 1.55 -0.36 -2.44
Nucleus 5.61 5.11 4.10 -10.44 -5.59 1.35 -1.45 -8.27 -8.59 -7.88 5.13 2.55 -7.33 -13.28 -10.63
Peroxisomes 5.09 -1.46 1.28 -9.77 1.98 -0.72 -3.80 -8.07 -7.46 -7.30 0.16 -3.76 -12.19 -10.21 -5.29
Vacuole 16.99 9.22 12.22 -16.93 2.52 4.44 -1.52 -11.31 -11.64 -11.16 11.19 2.68 -11.03 -19.91 -13.44
Vacuole Periphery -0.88 0.73 1.72 13.40 1.83 5.24 7.92 8.48 7.57 7.66 4.97 7.85 8.70 12.61 5.90