Standard name
Description RNA polymerase I enhancer binding protein; DNA binding protein that binds to genes transcribed by both RNA polymerase I and RNA polymerase II; required for termination of RNA polymerase I transcription; Reb1p bound to DNA acts to block RNA polymerase II readthrough transcription

Micrographs

ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 0 0 0 0 0.05 0 0 0 0 0 0 0 0.05 0.1 0.08 0.06 0 0 0 0 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 0 0 0 0.05 0 0 0.07 0.08 0 0 0 0 0.05 0 0 0 0 0 0 0 0
Nucleus 0.94 0.93 0.93 0.94 0.84 0.77 0.73 0.62 0.63 0.66 0.68 0.92 0.89 0.8 0.78 0.8 0.75 0.9 0.91 0.9 0.74 0.6 0.55
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus 0.14 0.1 0.07 0.07 0.15 0.26 0.3 0.46 0.42 0.46 0.42 0.16 0.16 0.23 0.1 0.16 0.16 0.05 0 0 0.16 0.23 0.22
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vac/Vac Membrane 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0.05 0 0.05 0 0 0 0.07 0.12 0.14
ensLOC DeepLoc
Localization WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 WT1 WT2 WT3 AF100 AF140 AF180
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 1 3 3 2 3 5 1 1 0 0 1 0 3 5 1 0 3 3
Bud Neck 0 0 1 0 2 1 3 6 4 5 2 0 0 0 0 0 0 0 0 0 1 0 1
Bud Site 0 0 0 0 0 4 5 8 5 10 9 0 1 2 0 0 0
Cell Periphery 0 1 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1 4 7 11 20 6 12 6 10 10 8 11 23 42 10 7 6 0 0 2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 3 0 0 1 0 1 1
Endosome 0 2 1 0 1 0 0 1 0 0 1 1 1 0 1 0 3 0 2 3 1 2 5
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0 0 1 1 1 0 1 0 1 2
Mitochondria 0 8 0 4 4 16 17 19 31 42 19 3 2 2 6 2 2 1 0 1 0 4 11
Nucleus 257 245 302 277 363 236 323 276 277 360 353 334 378 346 97 94 100 248 250 327 237 254 248
Nuclear Periphery 0 0 0 3 3 4 7 3 6 9 2 2 2 1 1 2 1 0 0 0 0 0 6
Nucleolus 37 25 24 20 64 80 132 206 182 250 220 59 67 100 13 19 22 13 6 13 50 97 102
Peroxisomes 0 1 1 0 0 0 0 0 0 0 0 0 0 3 0 0 0 0 0 0 0 1 0
SpindlePole 0 1 0 0 1 0 2 0 2 1 3 1 2 4 0 1 1 3 1 2 1 0 4
Vac/Vac Membrane 3 0 2 1 1 1 1 2 3 2 6 0 4 1 6 2 7 2 4 5 24 51 64
Unique Cell Count 272 263 324 295 431 307 440 444 437 546 522 364 425 435 125 117 134 275 276 366 321 424 455
Labelled Cell Count 298 287 338 316 459 349 505 530 522 692 628 412 481 502 135 130 146 275 276 366 321 424 455


Nucleus

(Huh et al. Nature 2003 425:686)
Screen WT1 WT2 WT3 RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3 AF100 AF140 AF180
Mean Cell GFP Intensity (1e-4) 15.9 15.3 12.9 12.4 13.0 10.1 10.7 10.8 11.0 10.1 11.4 13.8 13.6 12.9 15.3 17.7 14.9 13.0 13.7 13.8
Std Deviation (1e-4) 6.4 5.7 3.8 6.2 4.6 3.9 3.9 3.6 4.0 3.9 4.3 4.6 5.1 4.7 8.5 7.4 6.0 4.5 4.8 4.0
Intensity Change (Log2) -0.06 0.01 -0.36 -0.27 -0.26 -0.23 -0.36 -0.18 0.09 0.07 -0.01 0.24 0.45 0.21 0.01 0.08 0.1


Localization RAP60 RAP140 RAP220 RAP300 RAP380 RAP460 RAP540 RAP620 RAP700 HU80 HU120 HU160 rpd3Δ_1 rpd3Δ_2 rpd3Δ_3
Cortical Patches 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Neck 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Bud Site 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cell Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Cytoplasm 1.2 1.8 -0.2 0.5 -0.9 0.1 -0.3 -0.7 0.7 2.2 4.2 0 0 0
Endoplasmic Reticulum 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Endosome 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Golgi 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Mitochondria 0 0 4.2 3.6 3.8 4.9 5.1 3.5 0 0 0 0 0 0
Nucleus 0.3 -3.8 -5.8 -7.0 -9.8 -9.5 -9.1 -8.7 -0.7 -2.0 -5.3 -4.7 -3.9 -5.5
Nuclear Periphery 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Nucleolus -0.3 3.2 6.3 7.7 11.7 10.5 11.8 10.8 3.5 3.5 5.7 1.0 2.8 2.9
Peroxisomes 0 0 0 0 0 0 0 0 0 0 0 0 0 0
SpindlePole 0 0 0 0 0 0 0 0 0 0 0 0 0 0
Vacuole 0 0 0 0 0 0 0 0 0 0 0 0 0 0


Endocytosis
Temp Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Vph1-GFP)
37℃
RT
Cell Cycle Omics

CYCLoPs (Reb1-GFP)
Gene / Allele Actin Patch (Sac6-tdTomato) Cortical Patch (Sla1-GFP) Late Endosome (Snf7-GFP) Vacuole (Sac6-tdTomato)
Gene Images
Gene Images
Images are not yet available
Images are not yet available